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All Journal Padjadjaran Journal of Dentistry Paediatrica Indonesiana Molecular and Cellular Biomedical Sciences (MCBS) Scientific Dental Journal Jurnal Abdimas Kesehatan Terpadu

Erni Erfan, Erni

Universitas Trisakti

Author-ID : 614433

Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Articles

Squamous cell carcinoma of the tongue in Sprague-Dawley rats using 4-nitroquinoline 1-oxide model Erfan, Erni; N., Nafrialdi; Ekawuyung, Puspita
Padjadjaran Journal of Dentistry Vol 28, No 1 (2016): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Background. 4-nitroquinoline 1-oxide (4NQO) is a water soluble carcinogen which is often used to induce cancer in oral cavity. Objective. The purpose of this study was to know the incidence of histopathological lesions in the tongue of rats after administration of 4-nitroquinoline 1-oxide (4NQO) for a model of oral carcinogenesis in the animal experimental laboratory. Materials and Methods. This study was conducted in the Department ofÂ Pathological Anatomy, Faculty of Medicine, University of Indonesia (FMUI). 4NQO (30 ppm) was administered by drinking water ad libitum to the Sprague Dawley (SD) rats during different time inductions (4-9 and 36-46 weeks). The present of tumor in oral cavity of the 4NQO-treated rats were observed macroscopically and microscopically. Tumor volume were measured based on the formula established by G. Carlsson. The degree of histological changes was determined according to observation towards their Hematoxylin and Eosin (HE) staining results.Â Results. Dysplasia and invasive squamous cell carcinoma (SCC) were respectively found in the tongue of the group given 4NQO for 4-9 and 36-46 weeks. The tongue from rats (4NQO for 36-46 weeks) exhibited whitish protuberant nodular lesions with the longest diameter was 17 mm. The histopathological lesion incidences for dysplasia and invasive SCC respectively were 25% (5/20) and 75% (15/20). The rats had tumor in their tongues with variation of degree alteration which proportional to the induction time.Â The extension of 4NQO induction time increasing the degree of change in the tumor. Conclusion. 4NQO represents a good model of carcinoma of tongue and gives consistence results with histological changes degree being related to duration of exposure.

Inhibiting ability of benzathine penicillin G towards group A Streptococcus Î² -hemolyticus in 21 and 28 days after a single intramuscular injection Bambang Madiyono; Mulyadi M Djer; Sudigdo Sastroasmoro; Amin Subandrio; Erni Erfan
Paediatrica Indonesiana Vol 43 No 4 (2003): July 2003
Publisher : Indonesian Pediatric Society

Background The effectiveness of a single intramuscularbenzathine penicillin G as a secondary prevention of acute rheu-matic fever after 28 days is still controversial. The latest studyshowed that serum penicillin concentration was below protectivelevel in 4 weeks after a single intramuscular injection.Objective This study was a preliminary study to compare the in-hibiting ability of benzathine penicillin G towards group A Strepto-coccus Î² -hemolyticus in 21 and 28 days after a single IM injectionby using Mueller-Hinton broth method.Methods The inhibiting ability was measured by the serum in-hibitory concentration and serum bactericidal concentration aftera single intramuscular injection of benzathine penicillin G 1.2 mil-lion units.Results The subjects were 28 patients, with mean age of 15.5years (SD 3.4). The numbers of male and female were equal. Meanserum inhibitory concentration in 21 days after a single injectionwas 926.4 (SD 3080.1) and after 28 days was 1540.9 (SD 4275.1),which was not significantly different (p=0.0662). Mean serum bac-tericidal concentration in 21 days after IM injection was 1579.6(SD 4265.7) and in 28 days was 2417 (SD 6849.4) and it was notstatistically different (p=0.2276).Conclusion This study concluded that there was no significantdifference in the inhibiting ability of benzathine penicillin G to-wards group A Streptococcus Î² -hemolyticus between 21 and 28days after a single intramuscular injection

Choline-deficient High-fat Diet-induced Steatohepatitis in BALB/c Mice Saut Horas Hatoguan Nababan; Seruni Tyas Khairunissa; Erni Erfan; Nafrialdi Nafrialdi; Ening Krisnuhoni; Irsan Hasan; Rino Alvani Gani
Molecular and Cellular Biomedical Sciences Vol 5, No 2 (2021)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v5i2.193

Background: Non-alcoholic steatohepatitis (NASH) is an expanding cause of chronic liver disease worldwide, including Indonesia, with higher risk progression to cirrhosis and hepatocellular carcinoma. Preclinical experiments using several mice models have been conducted to clarify its complex pathogenesis. This study was designed to investigate whether BALB/c mice on a choline-deficient high-fat diet can be used as a model for NASH. Materials and Methods: BALB/c male mice were fed choline-deficient L-amino acid-defined high-fat diet (CDAHFD) or a standard diet for six weeks. The body and liver weights, liver histology, and plasma biochemistry were analyzed. The relative expression levels of tumor necrosis factor (TNF)α, transforming growth factor (TGF)β1, collagen-1α1 (COL1α1), glutathione peroxidase 1 (GPx1), and uncoupling protein 2 (UCP2) genes in the livers were analyzed using a two-step real time-polymerase chain reaction. Liver fatty acids composition was analyzed using gas chromatography with flame ionization detector (GC-FID). Results: CDAHFD induced steatohepatitis in BALB/c mice with increased plasma levels of alanine aminotransferase. The liver of CDAHFD-fed BALB/c mice showed upregulated relative expression levels of TNFα, TGFβ1, COL1α1, GPx1, and UCP2 genes. The liver fatty acid analysis showed a significant accumulation of saturated fatty acids (SFAs) and an increased ratio of n-6/n-3 polyunsaturated fatty acids (PUFAs) in the livers of CDAHFD-fed BALB/c mice. Conclusion: This study suggests that CDAHFD can induce steatohepatitis in BALB/c mice and therefore may be used as NASH mice model.Keywords: steatohepatitis, fatty liver, choline-deficient high fat diet, BALB/c

Enzymatic Activity of Bromelain Isolated Pineapple (Ananas comosus) Hump and Its Antibacterial Effect on Enterococcus faecalis Dewi Liliany; Armelia Sari Widyarman; Erni Erfan; Janti Sudiono; Melanie Sadono Djamil
Scientific Dental Journal Vol. 2 No. 2 (2018): May
Publisher : Faculty of Dentistry, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26912/sdj.v2i2.2540

Background: Enterococcus faecalis is the frequent cause of oral infections, such as periodontitis, infected root canals, and peri-radicular abscesses. Pineapple (Ananas comosus) fruit contains bromelain, one of proteolytic enzymes associated with several health benefits. Bromelain has been shown to promote healthy digestion, stimulate the immune system, improve cardiovascular conditions, and accelerate wound healing. Bromelain compounds possess anti-inflammatory and anticancer properties and exhibit antibacterial activity. Objective: To analyze the enzymatic activity of bromelain extracted from pineapple hump and investigate the antibacterial effect of bromelain against E. faecalis. Methods: Pineapple hump was dried and extracted with maceration technique. Further purification was obtained by ammonium sulfate fractionation, dialysis and ion exchange chromatography. Minimum inhibitory concentration (MIC) tests using diffusion and dilution techniques tested the antibacterial activity of the bromelain extract on E. faecalis. A one-way analysis of variance (ANOVA) test analyzed the significance of the differences in the E. faecalis inhibition zones after treatment with a range of bromelain extract concentrations. Differences were considered statistically significant if p < 0.05. Results: The specific activity of bromelain in the crude extract was 62.89 U/mg. Furthermore, bromelain activity using ammonium sulfate fractionation was 50.99 U/mg, dialysis was 54.59 U/mg, and ion exchange chromatography was 152.38 U/mg. The bromelain extract showed effective inhibitory and bactericidal activity against E. faecalis. The results of the inhibition test using a bromelain extract purified by ion exchange chromatography demonstrated that a concentration as small as 12.5% was effective in inhibiting the growth of E. faecalis (p < 0.05). Conclusion: The highest enzymatic activity of bromelain was found after purification with ion exchange chromatography. Bromelain exerted an antibacterial effect against a potent endodontic pathogen, but further studies are needed to explore this effect.

Penyuluhan Tatalaksana Awal Trauma Gigi pada Anak-anak Guru dan Wali Murid RA Al-Muttaqin Jeddy; Dwimega, Arianne; Tegar Badruzzaman, Idham; Erfan, Erni; Ihsan Rizal, Muhammad; Anggraeni, Rezky
JURNAL ABDIMAS KESEHATAN TERPADU Vol. 2 No. 2 (2023): Jurnal Abdimas Kesehatan Terpadu
Publisher : Fakultas Kedokteran Gigi, Universitas Trisakti bekerjasama dengan Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jakt.v2i2.18791

Anterior tooth trauma often occurs in children because children are more active than adults and their coordination and assessment of conditions are not good enough, so they often fall while learning to walk, run, play and exercise. Trauma that occurs to a child's teeth can interfere speech, chewing, aesthetics and the eruption of permanent teeth, thereby disrupting the growth and development of the teeth and jaw. Therefore, it is important to pay attention to the approach regarding the first treatment of trauma to primary teeth for parents and children. The choice of Community Service target at RA Al-Muttaqin was due to a request from the school principal as community leader. Education and training activities were carried out on July 27 2023 for a total of 20 teachers and parents. The aim of this activity is to increase knowledge and motivate changes in attitudes and behavior in maintaining oral health and regarding first care for primary tooth trauma. The initial assessment of knowledge and attitudes regarding primary care for trauma to primary teeth and the mouth of teachers was carried out through a pre-test. Counseling on how to maintain healthy teeth and mouth, including dental and oral health problems, prevention methods, as well as first care for primary tooth trauma by the presenter as well as educational videos. As an evaluation, a post test was carried out. The evaluation of the post test results showed an increase in scores. Recommendations for continuing this training activity in other elementary schools, especially for teachers in the school environment.

Background. 4-nitroquinoline 1-oxide (4NQO) is a water soluble carcinogen which is often used to induce cancer in oral cavity. Objective. The purpose of this study was to know the incidence of histopathological lesions in the tongue of rats after administration of 4-nitroquinoline 1-oxide (4NQO) for a model of oral carcinogenesis in the animal experimental laboratory. Materials and Methods. This study was conducted in the Department of Pathological Anatomy, Faculty of Medicine, University of Indonesia (FMUI). 4NQO (30 ppm) was administered by drinking water ad libitum to the Sprague Dawley (SD) rats during different time inductions (4-9 and 36-46 weeks). The present of tumor in oral cavity of the 4NQO-treated rats were observed macroscopically and microscopically. Tumor volume were measured based on the formula established by G. Carlsson. The degree of histological changes was determined according to observation towards their Hematoxylin and Eosin (HE) staining results. Results. Dysplasia and invasive squamous cell carcinoma (SCC) were respectively found in the tongue of the group given 4NQO for 4-9 and 36-46 weeks. The tongue from rats (4NQO for 36-46 weeks) exhibited whitish protuberant nodular lesions with the longest diameter was 17 mm. The histopathological lesion incidences for dysplasia and invasive SCC respectively were 25% (5/20) and 75% (15/20). The rats had tumor in their tongues with variation of degree alteration which proportional to the induction time. The extension of 4NQO induction time increasing the degree of change in the tumor. Conclusion. 4NQO represents a good model of carcinoma of tongue and gives consistence results with histological changes degree being related to duration of exposure.

Co-Authors Amin Subandrio Anggraeni, Rezky Arianne Dwimega Bambang Madiyono Dewi Liliany Margaretta, Dewi Liliany Ening Krisnuhoni Ihsan Rizal, Muhammad Irsan Hasan Janti Sudiono Jeddy Melanie Sadono Djamil Mulyadi M Djer Nafrialdi Nafrialdi Puspita Ekawuyung, Puspita Rino Alvani Gani Saut Horas Hatoguan Nababan Seruni Tyas Khairunissa Sudigdo Sastroasmoro Tegar Badruzzaman, Idham Widyarman, Armelia Sari

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