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All Journal PROSIDING SEMINAR KIMIA JURNAL INDRIA (Jurnal Ilmiah Pendidikan Prasekolah dan Sekolah Awal) JURNAL ATOMIK
Winni Astuti, Winni
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Energy Environmental Science

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ISOLASI DAN KARAKTERISASI LIPASE DARI KECAMBAH BIJI ALPUKAT (Persea americana Mill) Sya'bani, Nurillah; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 2 No 2 (2017)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.716 KB)

Abstract

Isolation and determination of optimum lipase conditions from Avocado seeds germination (Persea americana Mill) have been done. The purpose of this study is to determine the optimum working conditions and lipase specific activity of avocado seeds germination. Lipase was isolated using homogenization and centrifugation techniques. The crude extract of the enzyme containing the lipase produced from avocado seeds germination was used for the determination of total protein concentration and tested its activity on the pH conditions, temperature, and optimum substrate concentration with different with various variations. Determination of total concentration was done by Bradford method, while the determination of optimum working conditions and lipase specific activity was performed by titrimetric method. The results showed that total protein concentration in avocado seeds germination was 458 μg / mL. The optimum working conditions on avocado seed sprouts are the degree of acidity (pH) 6, temperature 35 oC, and 1.5% v/v substrate concentration. The lipase specific activity of avocado seeds germination is 144,1 U / mg.
Dampak Rasio Guru terhadap Prestasi Belajar Siswa di RA Yaspi Muneng 2 Salsabila, Ervina; Kesuma, Yonanda Meisya; Astuti, Winni; Panuti, Putri Rahayuning
JURNAL INDRIA (Jurnal Ilmiah Pendidikan Prasekolah dan Sekolah Awal) Vol 10 No 2 (2025): September
Publisher : Universitas Muhammadiyah Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24269/jin.v10i2.12250

Abstract

Early childhood education is the main foundation for children to develop in the future. Optimal interaction with the learning environment is essential to support overall growth and development. One crucial factor in supporting this process is the ratio between educators and students. This study aims to investigate the impact of the teacher-student ratio on student academic performance at RA Yaspi Muneng 2. The research approach used is qualitative descriptive with a case study method. Data collection techniques include classroom observation, in-depth interviews with teachers and parents, and documentation of student learning outcomes. The research findings indicate that a single teacher guiding two age groups in one classroom faces difficulties in providing individual attention, limitations in evaluating student progress, and administrative challenges. Interviews with parents also revealed that students' development differs from that of their siblings attending different schools, with parents citing the limited number of educators as a contributing factor. An unfavorable teacher-student ratio significantly impacts the effectiveness of learning and student academic performance.
SKRINING BAKTERI DARI AIR SUNGAI KARANG MUMUS SEBAGAI PENGHASIL ENZIM PROTEASE, SELULASE DAN LIPASE Amantha, Dhita Novalia; Astuti, Winni; Marliana, Eva
JURNAL ATOMIK Vol 10 No 2 (2025)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v10i2.1402

Abstract

Bacteria from polluted waters have the potential to produce extracellular hydrolase enzymes, namely protease enzymes, cellulase and lipase. This study was conducted to isolate bacteria from the water of the Karang Mumus River around Segiri Market, Samarinda as a producer of hydrolase enzymes. The bacteria were isolated in a nutrient-dense agar (NA) medium by the streak method. The results of bacterial isolation were obtained from 14 single colonies of bacteria. Single colonies of bacteria are selected to produce hydrolase enzymes in the form of proteases, cellulases and lipases. The results of the selection of hydrolase enzyme-producing bacteria were 3 protease-producing isolates, 10 cellulase-producing isolates, and 5 lipase-producing isolates.
SELEKSI AMILASE PENDEGRADASI PATI MENTAH BAKTERI DARI AIR GALIAN BEKAS TAMBANG BATU BARA DI DESA LOA BAHU Akbar, Akbar; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 10 No 2 (2025)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/ja.v10i2.1546

Abstract

Raw starch-degrading amylase (RSDA) is a type of hydrolase enzyme that can degrade raw starch into glucose without going through the heating and gelatinization process. This study was conducted to select RSDA-producing bacteria from ex-coal mine water samples in Loa Bahu Village, Sungai Kunjang, Samarinda, with various types of raw starch such as rice, wheat, corn, potatoes, sago, and cassava. The selection of bacteria-producing RSDA enzymes was carried out using agar nutrient media containing raw starch, the positive test was marked by the formation of a clear zone around the bacterial colony after the addition of iodine solution. A total of 13 bacterial isolates (AT 1 - AT 13) were tested qualitatively by RSDA. The selection results showed that 4 bacterial isolates were capable of degrading raw rice starch, 4 bacterial isolates were capable of degrading raw corn starch, 2 bacterial isolate was capable of degrading raw cassava starch, 1 bacterial isolate was capable of degrading raw potato starch, 1 isolate was capable of degrading raw wheat starch, and 2 bacterial isolates were able to degrade raw sago starch. The bacterial isolate with the code AT3 is capable of degrading raw starch of rice, corn, potatoes, wheat and sago.
Co-Authors A. A. R, Muhammad Taufik Admaja, Junior Try Afandi, Irvan Ahmad Suryadi, Ahmad Akbar Akbar Alawiyah, Dessy Noor Amalia, Elfiza Amantha, Dhita Novalia Anggriani, Yusnia Wati Aristina, Reza Fauziah Arwidhiah, Nanda Az-zahra, Deannisa Khoiru Chairul Saleh Djihan Ryn Pratiwi, Djihan Ryn Erwin Erwin Eva Marliana, Eva Fahmi, Ismi Haeruddin Haeruddin Hairani, Rita Kesuma, Yonanda Meisya Kusumawardani, Niken Marliani, Nelly Mikdariawan, Adha Mutma Inna Nasution, Safridah Hannum Noor Hindryawati, Noor Nur, Istianti Irfa'a Nursanti, Oktavia Widya Panuti, Putri Rahayuning puah, Siti Mar Purnamasari, Ade Rezki Putri, Amanda Aulia Rabiatul Adawiyah Rahimah, Priliza Rahmaniah, Rahmaniah Rakasiwi, Dea Rudi Kartika, Rudi Ruga, Ritbey Saibun Sitorus Salsabila, Ervina Shafarini, Eno Julia Siti Nurjanah Sya'bani, Nurillah Trisnawati, Eka Elly Usdin, Muhammad Sadam Utami, Nanda Putri Vera, Hendrica Mini Wahyu Pratama