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ISOLASI DAN KARAKTERISASI LIPASE DARI KECAMBAH BIJI ALPUKAT (Persea americana Mill) Sya'bani, Nurillah; Astuti, Winni; Pratiwi, Djihan Ryn
JURNAL ATOMIK Vol 2 No 2 (2017)
Publisher : Jurusan Kimia FMIPA UNMUL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.716 KB)

Abstract

Isolation and determination of optimum lipase conditions from Avocado seeds germination (Persea americana Mill) have been done. The purpose of this study is to determine the optimum working conditions and lipase specific activity of avocado seeds germination. Lipase was isolated using homogenization and centrifugation techniques. The crude extract of the enzyme containing the lipase produced from avocado seeds germination was used for the determination of total protein concentration and tested its activity on the pH conditions, temperature, and optimum substrate concentration with different with various variations. Determination of total concentration was done by Bradford method, while the determination of optimum working conditions and lipase specific activity was performed by titrimetric method. The results showed that total protein concentration in avocado seeds germination was 458 μg / mL. The optimum working conditions on avocado seed sprouts are the degree of acidity (pH) 6, temperature 35 oC, and 1.5% v/v substrate concentration. The lipase specific activity of avocado seeds germination is 144,1 U / mg.
Dampak Rasio Guru terhadap Prestasi Belajar Siswa di RA Yaspi Muneng 2 Salsabila, Ervina; Kesuma, Yonanda Meisya; Astuti, Winni; Panuti, Putri Rahayuning
JURNAL INDRIA (Jurnal Ilmiah Pendidikan Prasekolah dan Sekolah Awal) Vol 10 No 2 (2025): September
Publisher : Universitas Muhammadiyah Ponorogo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24269/jin.v10i2.12250

Abstract

Early childhood education is the main foundation for children to develop in the future. Optimal interaction with the learning environment is essential to support overall growth and development. One crucial factor in supporting this process is the ratio between educators and students. This study aims to investigate the impact of the teacher-student ratio on student academic performance at RA Yaspi Muneng 2. The research approach used is qualitative descriptive with a case study method. Data collection techniques include classroom observation, in-depth interviews with teachers and parents, and documentation of student learning outcomes. The research findings indicate that a single teacher guiding two age groups in one classroom faces difficulties in providing individual attention, limitations in evaluating student progress, and administrative challenges. Interviews with parents also revealed that students' development differs from that of their siblings attending different schools, with parents citing the limited number of educators as a contributing factor. An unfavorable teacher-student ratio significantly impacts the effectiveness of learning and student academic performance.