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All Journal Biology, Medicine, & Natural Product Chemistry BIOMA Jurnal Ilmiah Kedokteran Wijaya Kusuma Jurnal Abdidas Jurnal Pengabdian Masyarakat Kedokteran Calvaria Medical Journal
akhmad sudibya, akhmad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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USE OF THE BACTERIAL NAMES ANAYSIS ACCORDING TO HISTORICAL AND ETYMOLOGICAL ASPECTS TO FACILITATE THE MEMORIZATION sudibya, akhmad
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 3, No 1 (2014): Edisi Maret 2014
Publisher : Universitas Wijaya Kusuma Surabaya

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Abstract

Medical students should know two aspects of every bacterial names. The two aspects are etymological aspect and historical aspect. Knowledge of these aspects would facilitate the students in memorizing bacterial names.
THE THREE OF DYES TO SEE METACROMATIC GRANULA Sudibya, Akhmad
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 1, No 2 (2012): Edisi Oktober 2012
Publisher : Universitas Wijaya Kusuma Surabaya

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Abstract

There are two stains in addition to  Neisser’s Stain to detect metachromatic granules. The two stains are Albert’s Diphtheria Stain and Loeffler’s Alkalin Methylene Blue Stain for Metachromatic Granules. They should be taught to medical students. 
Uji Widal (Widal Test) Sudibya, Akhmad
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 1, No 1 (2012): Edisi Juli 2012
Publisher : Universitas Wijaya Kusuma Surabaya

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Abstract

Widal Test is one of the application of diagnostic immunology in the diagnosis of typhoid fever. Widal Test must be taught at the school of medicine although some physicians opines that this test is quite obsolete. In real life there are stillmany physicians in developing countries who consider this test as very instrumental in the diagnosis of typhoid fever.
THE POTENCY OF Candida albicans PROTEIN EXTRACT AS BIORESEPTOR ON IMMUNOCENCOR TO DIAGNOSE CANDIDIASIS Masfufatun, Masfufatun; Sudibya, Akhmad; Kumala, Nur
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 3, No 2 (2014): edisi Oktober 2014
Publisher : Universitas Wijaya Kusuma Surabaya

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Abstract

So far, diagnosing on candidiasis has still been limited in standard blood culture. The traditional method of this microbiology culture is less sensitive, many patients with candidasis infection have negative blood culture, and need a long time. This case encourage many researchers to use another alternative methode to diagnose. The purpose of this research is to examine the potency of Candida albicans protein extract as bioreseptor on immunocencor to  detect  Candida albicans and its biofilm in the blood of candidiasis patients. The research method which is used is descriptive with the following steps : (1) isolating enzim from the liquid of digestive gland of snail (Achatina fulica); (2) extraction of protein candida albicans  through  enzimatis and mechanic methods and (3) Analyzing the protein extract as bioreseptor through immunodot assay.The research result shows that the snail enzyme has the protein content 1.35 mg/ml and the specific activity 1.96 unit/mg. This snail enzyme can hydrolyze  the cell wall of Candidia albicans  and, with the help oh sonication, produce extract of planktonic extracel protein (PEP) and biofilm (PEB), extract of planktonic intracell protein (PIP), and biofilm (PIB), with each protein content 1.44; 1.29; 1.29 and 1.21 mg/ml. The characeristic of biofilm intracell protein (PIB) is antigenic on antibody anti-candida (positive control) with giving red spot on immunodot assay. Immunodot assay can distinguish negative control serum (health man) and positive Candidiasis control by using antigen 1 ng/nl and 50nl serum.
Penyuluhan Efektivitas Pemanfaatan Daun Kelor (Moringa oleifera Lamk.) terhadap Imunitas Tubuh di Puskesmas Dukuh Kupang Surabaya Listyawati, Agusniar Furkani; Sudibya, Akhmad; Soekanto, Ayly; Tania, Putu Oky Ari; Al-Aska, Andra Agnez; Hardiyono, Hardiyono; Simamora, Dorta; Tjandra, Lusiani
Jurnal Abdidas Vol. 5 No. 4 (2024): August, Pages 301 - 449
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdidas.v5i4.965

Abstract

Tanaman herbal memiliki khasiat untuk menyembuhkan atau mencegah berbagai gangguan kesehatan. Peningkatan imunitas tubuh dapat  dilakukan dengan memanfaatkan tanaman herbal. Salah satunya adalah daun kelor (Moringa oleifera Lamk.) yang memiliki kandungan fenol yang dapat meningkatkan sistem imun tubuh. Metode pengabdian masyarakat ini dengan memberikan penyuluhan kepada warga di wilayah Pukesmas Dukuh kupang Surabaya dengan memberikan ceramah dan informasi yang benar tentang manfaat daun kelor sebagai sistim imunitas yang alami. Kuisioner awal didapatkan persentase jumlah peserta yang mengerti tentang pemanfaatan daun kelor sebanyak 20% dari 30 orang peserta yang hadir dalam kegiatan ini. Setelah dilakukan penyuluhan peserta diberikan kuisioner akhir, sebanyak 98% dari total 30 peserta memahami tentang pemanfaatan daun kelor untuk kesehatan dan obat tradisional sehingga diharapkan seluruh peserta penyuluhan dapat mengaplikasikan di lingkungan tempat tinggalnya mengenai manfaat mengkonsumsi daun kelor sebagai salah satu makanan/obat yang dapat meningkatkan imunitas tubuh. Kesimpulan pengabdian masyarakat kali ini adalah kegiatan berjalan baik dan lancar serta antusias peserta pengabdian masyarakat sangat tinggi.
Prevalence of Enterobacteriaceae Producing Extended Spectrum Beta-Lactamase (ESBL) in Broiler Meat (Gallus domesticus) Sold in Regional Markets of West Surabaya Risnanda, Kadek Rio; Masfufatun, Masfufatun; Listyawati, Agusniar Furkani; Kuntaman, Kuntaman; Sudibya, Akhmad
Biology, Medicine, & Natural Product Chemistry Vol 12, No 2 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.122.607-610

Abstract

Broiler meat is a foodstuff that is easily damaged by bacterial growth. Several pathogenic bacteria from the Enterobacteriaceae family have also been found as contaminants in chicken meat. While Extended Spectrum Beta-Lactamases (ESBL) are enzymes that have the ability to hydrolyze antibiotics of the penicillin class, first, second, and third generation cephalosporins as well as the monobactam group. Based on this phenomenon, the researchers wanted to examine the prevalence of the ESBL-producing Enterobacteriaceae family in broiler chicken (Gallus domesticus) meat sold in the West Surabaya market. This research is a descriptive observational type using a cross-sectional approach. The population in this study was broiler chicken meat taken from broiler traders in several markets in West Surabaya which had met the inclusion and exclusion criteria with a large sample of 50 samples. Then the data were analyzed using the chi-square test. The results of the analysis showed that 13 samples produced ESBL. The prevalence of Enterobacteriaceae bacteria producing ESBL in broiler chicken (Gallus domesticus) meat in several markets in West Surabaya is 26% of the 50 samples used. The results of the chi-square test showed that there was no significant difference between locations where ESBL was found.
Pola Hidup Bersih untuk Meminimalkan Diare Akibat Escherichia Coli di Kelurahan Tembok Dukuh Surabaya Listyawati, Agusniar Furkani; Tania, Putu Oky Ari; Soekanto, Ayly; Rianti, Emillia Devi Dwi; Hardiyono, Hardiyono; Sudibya, Akhmad; Azka, Andra Agnes Al; Simamora, Dorta
Jurnal Pengabdian Masyarakat Kedokteran Vol 3, No 2 (2024): Mei
Publisher : Fakultas Kedokteran, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/abdimasku.3.2.68-73

Abstract

Pola hidup bersih adalah salah satu bagian dari menjaga kesehatan dan kebersihan tubuh yang dapat diukur dari kebersihan tangan. Kebersihan tangan dapat menjadi tolak ukur dalam kesehatan tubuh terutama untuk mencegah terjadinya diare dikarenakan bakteri Escherichia coli sebagai bakteri indikator terjadinya diare dapat dengan mudah menginfeksi tubuh apabila kebersihan tangan tidak terjaga. Tujuan kegiatan pengabdian masyarakat ini adalah untuk memberikan edukasi pola hidup bersih dan  meminimalkan kejadian diare terutama di musim hujan atau pancaroba ini. Setelah mendapatkan penyuluhan pada kegiatan pengabdian masyarakat ini tentang pola hidup bersih pada warga masyarakat di wilayah kelurahan Tembok Dukuh, menjadi mengerti pentingnya cuci tangan dan untuk selalu menerapkan pentingnya hidup bersih. Dari data hasil sampel swab tangan sebelum dan sesudah cuci tangan tidak menunjukkan adanya pertumbuhan Escherichia coli, hasil ini menunjukan bahwa pola hidup bersih telah menjadi kebiasaan bagi masyarakat Kelurahan Tembok Dukuh Surabaya.A clean lifestyle is one part of maintaining body health and cleanliness which can be measured by hand hygiene. Hand hygiene can be a benchmark for body health, especially in preventing diarrhea because Escherichia coli bacteria as an indicator of bacteria for diarrhea can easily infect the body if hand hygiene is not maintained. As an indicator bacteria for diarrhea can easily infect the body if hand hygiene is not maintained. A clean lifestyle is one part of maintaining body health and cleanliness which can be measured by hand hygiene. Hand hygiene can be a benchmark for body health, especially in preventing diarrhea because Escherichia coli bacteria community service activity aims to provide education on clean lifestyles and minimize the incidence of diarrhea, especially in this rainy or transition season. After receiving counseling during this community service activity about clean living patterns, residents in the Tembok Dukuh became aware of the importance of washing their hands and always implementing the importance of clean living. From the data from hand swab samples before and after washing hands, it did not show any growth of Escherichia coli. These results show that a clean lifestyle has become a habit for the people ward of Tembok Dukuh Subdistrict, Surabaya.
Hubungan Antara Perilaku Cara Membersihkan Daerah Urogenitalia Wanita Setelah Berkemih Dengan Kejadian Infeksi Saluran Kemih Pada Mahasiswi Angkatan 2021 di Salah Satu Universitas di Surabaya: Relationship Between Cleaning Behavior Urogenital Area After Urination With Events Urinary Tract Infections in Female Students Class of 2021 at a University in Surabaya Satriadewi, Ni Komang Aswindari Satriadewi; Ishartadiati, Kartika; Sudibya, Akhmad; Widyaningsih, Indah
Calvaria Medical Journal Vol 3 No 1 (2025): Edisi Juni 2025
Publisher : Institute for Research and Community Services (LPPM) of University of Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/cmj.v3i1.204

Abstract

Latar Belakang: Berdasarkan epidemiologi, Infeksi Saluran Kemih merupakan infeksi paling sering terjadi pada wanita. Infeksi Saluran Kemih (ISK) adalah penyakit yang disebabkan karena ada mikroorganisme yang berkembang biak di saluran kemih. Tujuan: untuk mengetahui hubungan perilaku higienitas genetalia dan kejadian infeksi saluran kemih pada mahasiswa di surabaya. Metode: Penelitian ini merupakan jenis penelitian analitik komparatif dengan menggunakan metode analisis data yaitu uji Chi-square. Pengumpulan data menggunakan kuesioner. Pengolahan data penelitian menggunakan bantuan SPSS. Hasil: Hasil penelitian ini menunjukkan bahwa dari 69 responden yang menerapkan cara pembersihan daeraj urogenitalia dengan benar dan terinfeksi saluran kemih adalah sebanyak 36 mahasiswi (58%) sedangkan responden yang membersihkan daerah urogenitalia dengan benar tetapi tidak terinfeksi saluran kemih sebanyak 26 orang (41,93%). Kondisi responden yang penerapan cara pembersihan daerah urogenitalia yang salah dan terinfeksi saluran kemih sebanyak 3 orang (42,85%). Dan responden yang penerapan cara pembersihan daerah urogenitalia salah tetapi tidak terinfeksi saluran kemih sebesar 4 orang (57,14%). Penelitian ini menggunakan uji Chi-square dengan uji alternatif yaitu uji Fisher. Karena nilai p- value α ≥ 0,05. Kesimpulan: Dari hasil data yang didapatkan bahwa tidak terdapat hubungan antara perilaku cara membersihkan daerah urogenitalia wanita dengan kejadian Infeksi Saluran Kemih pada mahasiswi angkatan 2021 di salah satu Universitas di Surabaya.
Detection of Enterobacteriaceae Lactose Fermenter Bacteria Producing Extended Spectrum Beta-Lactamase (ESBL) in Food Samples at Surabaya Parawidnyaningsih, Putu Ayu Aryanda; masfufatun, masfufatun; Listyawati, Agusniar Furkani; Kuntaman, Kuntaman; Sudibya, Akhmad
Bioma : Jurnal Ilmiah Biologi Vol 12, No 2: October 2023
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v12i2.16618

Abstract

Snacks sold on the side of the road are often purchased because they are practical and cheap. The condition of food sold in the open causes a decrease in food hygiene, so bacteria will easily contaminate, both due to flies infesting it and direct contact with the hands of people around it. One group of bacteria that can cause infection and often contaminate food is Enterobacteriaceae. These bacteria can produce Extended Spectrum Beta Lactamase (ESBL) enzymes that cause resistance to various antibiotics (MDR). This research aims to detecting ESBL-producing Enterobacteriaceae lactose fermenter bacteria in street food sold on the side of the road in the Dukuh Kupang market area, Surabaya. This study is descriptive observational research with a cross-sectional research design. A total of 50 snack food samples were grown on MacConkey agar containing cefotaxime at four micrograms per milliliter. Then an ESBL confirmation test was carried out using the double disk synergy test (DDST). The Result showed that 13 (26%) samples of snack foods were contaminated. ESBL-producing Enterobacteriaceae Lactose Fermenter bacteria are ubiquitous. The most contaminated snack food was cassava, while the non-contaminated snack food was stuffed tofu.
Co-Authors Andra Agnez Al Aska Ayly Soekanto Azka, Andra Agnes Al Dorta Simamora Emillia Devi Dwi Rianti, Emillia Devi Dwi Indah Widyaningsih Kartika Ishartadiati Kuntaman Kuntaman Listyawati, Agusniar Furkani Lusiani Tjandra Masfufatun Masfufatun Nur Kumala, Nur Parawidnyaningsih, Putu Ayu Aryanda Putu Oky Ari Tania Risnanda, Kadek Rio Satriadewi, Ni Komang Aswindari Satriadewi