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All Journal Biology, Medicine, & Natural Product Chemistry BIOMA Jurnal Ilmiah Kedokteran Wijaya Kusuma Briliant: Jurnal Riset dan Konseptual The Indonesian Journal of Infectious Diseases Jurnal Abdidas Berkala Penelitian Hayati Jurnal Pengabdian Masyarakat Kedokteran Gema Lingkungan Kesehatan Calvaria Medical Journal Prosiding Seminar Nasional COSMIC Kedokteran
Listyawati, Agusniar Furkani
Universitas Wijaya Kusuma Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Growth Pattern of Pseudomonas sp. by Using D-glucose concentration Variation in Growth Media Against Incubation Time Listyawati, Agusniar Furkani
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 5, No 2 (2016): Edisi September 2016
Publisher : Universitas Wijaya Kusuma Surabaya

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Abstract

This study was to determine the growth pattern of Pseudomonas sp. by administering various concentration of D-glucose to the growth medium and to see its effect on various incubation times. Addition of D-Glucose in the growth medium as a source of bacterial carbon that can support the cell growth process. The results showed that, the use of D-glucose with various concentration in growth media of Pseudomonas sp. affect the formation of bacterial cell growth patterns. This study showed that Pseudomonas sp. using D-glucose substrate for it’s cell growth. While the longer incubation time didn’t had effect for the cell growth pattern due to the ability of bacteria in consuming substrates that are added to the growth medium.
Kuat medan listrik AC dalam menghambat pertumbuhan koloni Staphylococcus aureus dan Escherichia coli Emilia Devi Dwi Rianti; Putu Oky Ari Tania; Agusniar Furkani Listyawati
Bioma : Jurnal Ilmiah Biologi Vol. 11 No. 1: April 2022
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v11i1.9561

Abstract

ABSTRAKStaphylococcus aureus adalah bakteri Gram positif yang merupakan  patogen penyebab peningkatan jumlah penyakit dan kematian setiap tahun. Bakteri ini berkolonisasi di hidung dan kulit manusia sehingga menyebabkan berbagai penyakit. Resistensi bakteri patogen terhadap antibiotik merupakan satu permasalahan besar yang membutuhkan strategi khusus dalam penanganannya. Salah satunya dengan penggunaan medan listrik AC digital sebagai pengendali pertumbuhan bakteri patogen S. aureus dan E. coli. Penelitian merupakan penelitian eksperimental dengan biakan bakteri S. aureus dan E. coli yang masing-masing dibagi menjadi 4 kelompok yaitu K0, K1, K2 dan K3 berdasarkan variasi kuat medan listrik AC yaitu 200 V/m, 400 V/m dan 600 V/m dengan waktu paparan 5 menit. Data diambil berupa jumlah koloni bakteri. Hasil penelitian menunjukkan adanya pengaruh kuat medan listrik terhadap pertumbuhan bakteri S. aureus dan E. coli. Semakin besar kuat medan magnet, maka pertumbuhan kedua bakteri semakin terhambat ditandai dengan kecilnya jumlah koloni yang hidup. Hasil uji statistik menunjukkan nilai p < 0,001 dengan uji ANOVA pada E. coli dan p < 0,001 menggunakan uji Kruskal-Wallis pada S. aureus. Kata Kunci: Escherichia coli; jumlah koloni; medan listrik AC; Staphylococcus                        aureus  ABSTRACTStrength of AC electricity for inhibiting of  Staphylococcus aureus and Escherichia coli colony growth. Staphylococcus aureus is a gram-positive bacteria as human pathogen, causing an increase in the number of diseases and deaths every year. The bacteria growing in the nose and skin of humans and can cause some illnesses. Resistance of pathogenic bacteria to antibiotics is one of the biggest problems and the typhical strategies are necessary, such as the use of AC electricity to inhibit the growth of S. aureus and E. coli. This research was an experimental study with S. aureus and E. coli bacteria is divided into 4 groups, each, there are K0, K1, K2 and K3 based on variations in the AC electric field, 200 V/m, 400 V/m and 600 V/m for 5 minutes. The study showed that there is effect of AC electricity to S. aureus and E. coli growth. The stronger AC electricity, the growth of those two bacteria is more inhibited. It is marked with smaller viable colony. Statistical test results obtained p value < 0.001 with ANOVA test on Escherichia coli and p < 0.001 using Kruskal-Wallis test on Staphylococcus aureus. Keywords : AC electricity; colony number; Escherichia coli; Staphylococcus aureus
Antibacterial and antibiofilm effect of silver and gold nanoparticles in Uropathogenic Escherichia coli Rini Purbowati; Sugiharto; Agusniar Furkani Listyawati; Masfufatun; Lusiani Tjandra; Noer Kumala Indahsari
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 27 No 2 (2022): June 2022
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.27.2.20222

Abstract

Nosocomial infections of the urinary tract are the most common nosocomial infections. Infectious diseases currently pose a life threatening due to the increasing phenomenon of infectious agent resistance. One of the factors known to cause resistance to broad-spectrum antimicrobials agent is the susceptibility to biofilm formation. The use of silver nanoparticles (NPs) is now regarded as one of the most promising strategies to target infections associated with biofilms. This study aims to determine the application of nanotechnology in the form of metal nanoparticles in targeting the formation of biofilms on infectious agents. The antibacterial and antibiofilm activity test was performed on a 96-well microtiter plate on uropathogenic Escherichia coli (UPEC) at concentrations of 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 ppm. The results showed the addition of silver and gold nanoparticles at different concentrations affected and inhibited the growth of UPEC and prevented the formation of biofilms, as indicated by a decrease in the OD value. In conclusion, silver and gold nanoparticles have antibacterial and antibiofilm effects on UPEC.
Dispersion of Carbon Monoxide Pollutant and The Effect of Health (Case Study on Frontage Road Surabaya by Gaussian Line Source Equation Model) Nurachmawati Meindah Sari; R. Azizah; Lilis Sulistyorini; Endrayana Putut Laksminto Emanuel; Emillia Devi Dwi Rianti; Fuad Ama; Sukma Sahadewa; Agusniar Furkani Listyawati; Ayly Soekanto; Hardiyono Hardiyono
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 11, No 2 (2022): SEPTEMBER 2022
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (406.797 KB) | DOI: 10.30742/jikw.v11i2.2416

Abstract

Air pollution was being a very important problem and danger for human life. This was related to diseases that arise due to motor vehicle emissions, especially carbon monoxide. Simulation of air dispersion models is the one way to study about air quality that is needed in this regard. This study aims to determine the distribution of carbon monoxide pollutants in Ahmad Yani's frontage and to anticipate the dangers of these pollutants to the health of the people living around the research location. This research discussed about the mathematical model of the dispersion of CO that emitted from cars that passed through the frontage road on the Ahmad Yani Street, Surabaya. The method used is direct observation in the field and numerical simulation using a mathematical model, Gaussian Line Source Equation Model (GLSEM). GLSEM had prepared based on the mechanism of transport of pollutants in dispersion, diffusion and advection. With GLSEM we calculated CO gas concentration values for certain heights downwind. We validated the model by comparing numerical results and measurements of CO concentration. We used the R2 test and we got an R2 close to one. We simulated GLSEM by used Fortran programming language and visualized it with Surfer. The results of the visualization in June showed that the pattern of CO gas dispersion was influenced by the direction and speed of the wind. The results obtained are that the distribution of CO pollutants in the Ahmad Yani frontage is horizontal/downwind. CO concentrations at night are higher than during the daytime. From the CO dispersion pattern, we had known that there were dangerous of air around the frontage for people health. We conclude that around the frontage road of the Ahmad Yani highway there is sufficient open air space so that the danger of CO pollutants being emitted can be minimized so that the health of the community, namely pedestrians, motorcycle drivers and the community around the location can be protected.
Pola Pertumbuhan Pseudomonas sp. dengan Menggunakan Variasi Konsentrasi D-glukosa dalam Media Pertumbuhan terhadap Waktu Inkubasi Agusniar Furkani Listyawati
Jurnal Ilmiah Kedokteran Wijaya Kusuma Vol 5, No 2 (2016): Edisi September 2016
Publisher : Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.018 KB) | DOI: 10.30742/jikw.v5i2.339

Abstract

This study was to determine the growth pattern of Pseudomonas sp. by administering various concentration of D-glucose to the growth medium and to see its effect on various incubation times. Addition of D-Glucose in the growth medium as a source of bacterial carbon that can support the cell growth process. The results showed that, the use of D-glucose with various concentration in growth media of Pseudomonas sp. affect the formation of bacterial cell growth patterns. This study showed that Pseudomonas sp. using D-glucose substrate for it’s cell growth. While the longer incubation time didn’t had effect for the cell growth pattern due to the ability of bacteria in consuming substrates that are added to the growth medium.
Penyuluhan Efektivitas Pemanfaatan Daun Kelor (Moringa oleifera Lamk.) terhadap Imunitas Tubuh di Puskesmas Dukuh Kupang Surabaya Listyawati, Agusniar Furkani; Sudibya, Akhmad; Soekanto, Ayly; Tania, Putu Oky Ari; Al-Aska, Andra Agnez; Hardiyono, Hardiyono; Simamora, Dorta; Tjandra, Lusiani
Jurnal Abdidas Vol. 5 No. 4 (2024): August, Pages 301 - 449
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdidas.v5i4.965

Abstract

Tanaman herbal memiliki khasiat untuk menyembuhkan atau mencegah berbagai gangguan kesehatan. Peningkatan imunitas tubuh dapat  dilakukan dengan memanfaatkan tanaman herbal. Salah satunya adalah daun kelor (Moringa oleifera Lamk.) yang memiliki kandungan fenol yang dapat meningkatkan sistem imun tubuh. Metode pengabdian masyarakat ini dengan memberikan penyuluhan kepada warga di wilayah Pukesmas Dukuh kupang Surabaya dengan memberikan ceramah dan informasi yang benar tentang manfaat daun kelor sebagai sistim imunitas yang alami. Kuisioner awal didapatkan persentase jumlah peserta yang mengerti tentang pemanfaatan daun kelor sebanyak 20% dari 30 orang peserta yang hadir dalam kegiatan ini. Setelah dilakukan penyuluhan peserta diberikan kuisioner akhir, sebanyak 98% dari total 30 peserta memahami tentang pemanfaatan daun kelor untuk kesehatan dan obat tradisional sehingga diharapkan seluruh peserta penyuluhan dapat mengaplikasikan di lingkungan tempat tinggalnya mengenai manfaat mengkonsumsi daun kelor sebagai salah satu makanan/obat yang dapat meningkatkan imunitas tubuh. Kesimpulan pengabdian masyarakat kali ini adalah kegiatan berjalan baik dan lancar serta antusias peserta pengabdian masyarakat sangat tinggi.
Prevalence of Enterobacteriaceae Producing Extended Spectrum Beta-Lactamase (ESBL) in Broiler Meat (Gallus domesticus) Sold in Regional Markets of West Surabaya Risnanda, Kadek Rio; Masfufatun, Masfufatun; Listyawati, Agusniar Furkani; Kuntaman, Kuntaman; Sudibya, Akhmad
Biology, Medicine, & Natural Product Chemistry Vol 12, No 2 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.122.607-610

Abstract

Broiler meat is a foodstuff that is easily damaged by bacterial growth. Several pathogenic bacteria from the Enterobacteriaceae family have also been found as contaminants in chicken meat. While Extended Spectrum Beta-Lactamases (ESBL) are enzymes that have the ability to hydrolyze antibiotics of the penicillin class, first, second, and third generation cephalosporins as well as the monobactam group. Based on this phenomenon, the researchers wanted to examine the prevalence of the ESBL-producing Enterobacteriaceae family in broiler chicken (Gallus domesticus) meat sold in the West Surabaya market. This research is a descriptive observational type using a cross-sectional approach. The population in this study was broiler chicken meat taken from broiler traders in several markets in West Surabaya which had met the inclusion and exclusion criteria with a large sample of 50 samples. Then the data were analyzed using the chi-square test. The results of the analysis showed that 13 samples produced ESBL. The prevalence of Enterobacteriaceae bacteria producing ESBL in broiler chicken (Gallus domesticus) meat in several markets in West Surabaya is 26% of the 50 samples used. The results of the chi-square test showed that there was no significant difference between locations where ESBL was found.
Pola Hidup Bersih untuk Meminimalkan Diare Akibat Escherichia Coli di Kelurahan Tembok Dukuh Surabaya Listyawati, Agusniar Furkani; Tania, Putu Oky Ari; Soekanto, Ayly; Rianti, Emillia Devi Dwi; Hardiyono, Hardiyono; Sudibya, Akhmad; Azka, Andra Agnes Al; Simamora, Dorta
Jurnal Pengabdian Masyarakat Kedokteran Vol 3, No 2 (2024): Mei
Publisher : Fakultas Kedokteran, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/abdimasku.3.2.68-73

Abstract

Pola hidup bersih adalah salah satu bagian dari menjaga kesehatan dan kebersihan tubuh yang dapat diukur dari kebersihan tangan. Kebersihan tangan dapat menjadi tolak ukur dalam kesehatan tubuh terutama untuk mencegah terjadinya diare dikarenakan bakteri Escherichia coli sebagai bakteri indikator terjadinya diare dapat dengan mudah menginfeksi tubuh apabila kebersihan tangan tidak terjaga. Tujuan kegiatan pengabdian masyarakat ini adalah untuk memberikan edukasi pola hidup bersih dan  meminimalkan kejadian diare terutama di musim hujan atau pancaroba ini. Setelah mendapatkan penyuluhan pada kegiatan pengabdian masyarakat ini tentang pola hidup bersih pada warga masyarakat di wilayah kelurahan Tembok Dukuh, menjadi mengerti pentingnya cuci tangan dan untuk selalu menerapkan pentingnya hidup bersih. Dari data hasil sampel swab tangan sebelum dan sesudah cuci tangan tidak menunjukkan adanya pertumbuhan Escherichia coli, hasil ini menunjukan bahwa pola hidup bersih telah menjadi kebiasaan bagi masyarakat Kelurahan Tembok Dukuh Surabaya.A clean lifestyle is one part of maintaining body health and cleanliness which can be measured by hand hygiene. Hand hygiene can be a benchmark for body health, especially in preventing diarrhea because Escherichia coli bacteria as an indicator of bacteria for diarrhea can easily infect the body if hand hygiene is not maintained. As an indicator bacteria for diarrhea can easily infect the body if hand hygiene is not maintained. A clean lifestyle is one part of maintaining body health and cleanliness which can be measured by hand hygiene. Hand hygiene can be a benchmark for body health, especially in preventing diarrhea because Escherichia coli bacteria community service activity aims to provide education on clean lifestyles and minimize the incidence of diarrhea, especially in this rainy or transition season. After receiving counseling during this community service activity about clean living patterns, residents in the Tembok Dukuh became aware of the importance of washing their hands and always implementing the importance of clean living. From the data from hand swab samples before and after washing hands, it did not show any growth of Escherichia coli. These results show that a clean lifestyle has become a habit for the people ward of Tembok Dukuh Subdistrict, Surabaya.
Perbedaan Daya Hambat Ekstrak Propolis dan Kunyit Putih terhadap Bakteri e. Coli pada Konsentrasi Minimum Putu Oky Ari Tania; Agusniar Furkani Listyawati; Emilia Devi Dwi Rianti; Ayly Soekanto
Prosiding Seminar Nasional COSMIC Kedokteran Vol 1 (2023): Prosiding Seminar Nasional COSMIC Kedokteran
Publisher : Lembaga Penelitian Dan Pengabdian Kepada Masyarakat Universitas Wijaya Kusuma Surabaya

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Abstract

Resistensi antibiotik akibat e. coll berkembang beberapa tahun belakangan. resistensi antibiotik ini dapat menjadi penyebab kematian akibat infeksi. amoksisilin dilaporkan memiliki penurunan aktivitas antibiotik terhadap e. coll. senyawa flavonoid diketahui mengandung fenol yang dapat mengganggu dan merusak membran sel (indryani et al). Senyawa ini didapatkan pada propolis maupun kunyit putih. penelitian ini bertujuan untuk melihat perbedaan data hambat antara ektsrak propolis dan kunyit putih pada konsentrasi hambat minumum sebesar 5%. Penelitian ini menggunakan penelitian deskriptif analitik dengan 2 kelompok perlakuan yaitu ekstrak propolis dan kunyit putih, dan 2 kelompok kontrol. Kelompok kontrol negatif menggunakan antibiotik amoksisilin. Penelitian ini menggunakan metode dilusi cakram dengan Hasil penelitian menunjukkan zona hambat pada ekstrak proplis bertipe radikal atau membentuk zona bening, dibandingkan ekstrak kunyit putih. Rata-rata diameter zona bening pada ekstrak propolis dan ekstrak kunyit putih adalah 8,93 mm dan 7,92 mm. Dari hasil penelitian menunjukkan bahwa ekstrak propolis memiliki potensi daya hambat lebih baik dibandingkan dengan ekstrak kunyit putih terhadap bakteri e. coli. Dibandingkan dengan kontrol positif yaitu pemberian antibiotik amoksisilin, terbukti ekstrak propolis maupun kunyit putih menunjukkan daya hambat yang lebih baik
Pengaruh Probiotik Dan Metabolit Lactiplantibacillus plantarum Dad-13 Terhadap Pertumbuhan dan Pembentukan Biofilm E. coli ATCC 8739 dan S. aureus ATCC 33591 Purbowati, Rini; Listyawati, Agusniar Furkani; Masfufatun, Masfufatun; Tjandra, Lusiani; Indahsari, Noer Kumala
The Indonesian Journal of Infectious Diseases Vol. 10 No. 2 (2024): The Indonesian Journal of Infectious Diseases
Publisher : Rumah Sakit Penyakit Infeksi Prof Dr. Sulianti Saroso

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32667/ijid.v10i2.291

Abstract

Latar belakang: Infeksi bakteri banyak terjadi di negara-negara berkembang, dengan angka kesakitan dan kematian yang meningkat. E. coli dan S. aureus merupakan bakteri penyebab infeksi serius pada berbagai penyakit yang terdapat di masyarakat serta di rumah sakit. Penggunaan antimikroba yang tidak tepat untuk mengobati infeksi bakteri telah menjadi penyebab utama timbulnya resistensi antibiotik pada banyak pathogen. Probiotik dan juga postbiotik dapat digunakan sebagai alternatif pengganti antibiotik. Tujuan: untuk mengetahui pengaruh probiotik dan postbiotic Lactiplantibacillus plantarum Dad-13 terhadap pertumbuhan dan pembentukan biofilm E. coli ATCC 8739 dan S. aureus ATCC 33591. Metode: penelitian ini merupakan penelitian eksperimental laboratories. Uji aktivitas antibakteri dan antibiofilm dari probiotik dan postbiotik L. plantarum subsp. plantarum Dad-13 terhadap E. coli ATCC 8739 dan S. aureus ATCC 33591 dilakukan dengan konsentrasi 25, 50, 75 dan 100 %.  Analisis terhadap perubahan pertumbuhan dan biofilm yang terbentuk diukur melalui serapan OD pada panjang gelombang 490 nm menggunakan microplates reader Hasil: Uji aktivitas antibakteri probiotik L. plantarum subsp. plantarum Dad-13 hanya sedikit menunjukkan penurunan pertumbuhan atau OD, kecuali pada konsentrasi 25 %  terhadap E. coli ATCC 8739. Uji aktivitas antibiofilm probiotik L. plantarum subsp. plantarum Dad-13 menunjukkan penurunan biofilm yang nyata baik terhadap E. coli ATCC 8739 maupun S. aureus ATCC 33591. Uji aktivitas antibakteri postbiotik L. plantarum subsp. plantarum Dad-13 menunjukkan hanya sedikit penurunan pertumbuhan atau OD, terhadap E. coli ATCC 8739 maupun S. aureus ATCC 33591. Uji aktivitas antibiofilm postbiotik L. plantarum subsp. plantarum Dad-13 juga hanya sedikit menunjukkan penurunan biofilm baik terhadap E. coli ATCC 8739 maupun S. aureus ATCC 33591 Kesimpulan: Probiotik L. plantarum subsp. plantarum Dad-13 menunjukkan pengaruh sebagai antibakteri yang lemah, namun menunjukkan antibiofilm yang kuat terhadap terhadap E. coli ATCC 8739 dan S. aureus ATCC 33591. Postbiotik L. plantarum subsp. plantarum Dad-13 menunjukkan pengaruh sebagai antibakteri yang lemah dan juga antibiofilm terhadap E. coli ATCC 8739 dan S. aureus ATCC 33591.
Co-Authors Abrori, Amiril akhmad sudibya, akhmad Al-Aska , Andra Agnez Andra Agnez Al Aska Ayly Soekanto Azka, Andra Agnes Al Dorta Simamora Emanuel, Endrayana Putut Laksminto Emilia Devi Dwi Rianti Emilia Devi Dwi Rianti Emillia Devi Dwi Rianti, Emillia Devi Dwi Fatimatuz Zahra, Fatimatuz Febtarini Rahmawati Fuad Ama Hardiyono , Hardiyono Hardiyono Hardiyono Kuntaman Kuntaman Lilis Sulistyorini Lusiani Tjandra Masfufatun Masfufatun Masfufatun Noer Kumala Indahsari, Noer Kumala Nurachmawati Meindah Sari Parawidnyaningsih, Putu Ayu Aryanda Putu Oky Ari Tania R. Azizah Rini Purbowati Risnanda, Kadek Rio Sugiharto Sukma Sahadewa, Sukma