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All Journal Jurnal Teknologi Laboratorium Medical Laboratory Technology Journal Meditory : The Journal of Medical Laboratory Jurnal Skala Kesehatan JCPS (Journal of Current Pharmaceutical Sciences) Jurnal Riset Kefarmasian Indonesia Indian Journal of Forensic Medicine & Toxicology Tropical Health and Medical Research Journal JPEMAS: Jurnal Pengabdian Kepada Masyarakat

leka lutpiatina, leka

Poltekkes Kemenkes Banjarmasin

Author-ID : 616250

Religion Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Other

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Lime Soap as Bacteria Reducer Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 4 No. 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Some people in South Kalimantan have used river water to fulfill their daily needs include washing the utensils. Wash the cutlery use river water can cause disease transmission. Squeezed lime has an antibacterial effect in inhibiting bacterial growth. Antibacterial dishwashing soap is effective in reducing pathogenic bacteria. This research is to know the difference of bacterial amount on the variation of contact time with soap. Know the difference of bacteria on the variation of concentration of lime soap and soap without lime. The study used the experimental method with pretest and posttest with control design. The independent variables are a lime soap with a concentration of 40%, 50%, 60% and variable of contact time 30 second, 60 second and 90 seconds. The dependent variable is the number of bacteria on the dinnerware of a dinner plate. The data obtained in this study is the data of measurement of the number of bacteria on the dinner plate. Data analyzed by Kruskal-Wallis test. MPN coliform examination of river water showed results >2400 CFU/ml sample. There were no significant differences in treatment with contact time variation of 30 seconds, 60 seconds and 90 seconds. The significance value is 0.796 (> 0.05). There were significant differences in the treatment of lime soap with concentration variations of 40%, 50%, 60%, and soap without lime. The value of significance is 0,000 (<0.05).

Potensi Ekstrak Rimpang Kencur (Kaempferia galanga L.) Menghambat Pertumbuhan Candida albicans Annisa Rahmi; Erpan Roebiakto; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 2 No. 2 (2016): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Candida albicans infection is the cause of candidiasis. Candidiasis treatment can be done with a variety of antifungal drugs, one of them is rhizome of kencur (Kaempferia galanga L.). The Rhizome of kencur is selected as a traditional medicine because it contains chemical compounds such as flavonoids, tannins, saponins and essential oil that serves as an antifungal. This study aimed to determine the minimal inhibitory and minimal killing power and also an influence of kencur rhizome extract on the growth of Candida albicans in vitro. This research was true experimental design with posttest only control group design with tube dilution method. Results of Minimal Inhibitory Concentrations (MICs) research showed there was no clarity at concentration of 20 mg/mL, 30 mg/mL, 40 mg/mL, and it shows clarity at concentration of 50 mg/mL and 60 mg/mL. Results of Minimum Bactericidal Concentrations (MBCs) showed the number of colonies at concentration of 20 mg/mL were 84 colonies, concentration of 30 mg/mL were 48 colonies, concentration of 40 mg/mL were 27 colonies, concentration of mg/mL were 12 colonies and concentration of 60 mg/mL were 0 colony. Based on linear regression test, the result showed significance value of 0.000

Mutu Bakteriologis Saus Tomat Pentol di Banjarbaru Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 2 No. 1 (2016): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Abstract: The food was used as a complementary flavor sauces include snacks bulb. Dressing materials according to SNI 01-7388-2009 must comply with the requirements of the parameter Staphylococcus aureus, MPN (Most probable number) Coliform, TPC (total plate count) and molds. This study aims to determine the percentage of tomato sauce snacks bulb in the area around the intersection of four Banjarbaru who do not qualify SNI 01-7388-2009 of parameters MPN Coliform and Staphylococcus aureus. The results showed MPN Coliform tomato sauce ranges from 0-240 and Staphylococcus aureus 5 x 101 to 6.5 x 103. Concluded percentage tomato sauce snacks bulb that does not qualify SNI 01-7388-2009 parameter is 60% MPN Coliform and Staphylococcus aureus 80%. Suggestions to determine the value of TPC (total plate count) and molds and other chemical parameters of tomato sauce.

Pewarnaan Gram Buffy Coat untuk Deteksi Awal Pasien Bakteremia leka lutpiatina
Medical Laboratory Technology Journal Vol. 1 No. 1 (2015): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Abstract: Bacteremia is one public health problem. Bacteremia was ranked as the tenth leading cause of death in the United States. Gram staining of buffy coat can be used for early detection of patients with bacteremia. Rapid diagnostic tests with these Gram staining can be interpreted within an hour and the research that has been conducted Richmond et al. 2002, have a sensitivity of 75% and specificity of 79%. This study aimed to study the sensitivity and specificity of Gram buffy coat with the gold standard blood culture media biphasic fever suspected patients. This study was an observational study with cross sectional study. The study population was suspected fever patients in hospitals Ratu Zalecha Martapura months from March to May 2013. The samples were suspect fever patients in hospitals Queen Zalecha Martapura March-May 2013 were taken by purposive with the inclusion criteria: Patients District General Hospital Ratu Zalecha Martapura, aged ≥10 years, had fever ≥ 5 days, had symptoms of abdominal pain, willing to follow the study. Gram staining microscopic results were analyzed by application epicalc. Microscopic results buffy coat Gram staining Gram-negative rods are found as many as seven (26%) and were not found Gram-negative rods as many as 20 (74%). Culture results of this study are Gram negative bacterial growth as much as 6 (22%) and no growth as many as 21 (78%). Conclusion The results of microscopic Gram stain buffy coat has a value of 67% sensitivity and 86% specificity.

Pengaruh Variasi Suhu Awal Reagen terhadap Kadar Glukosa Darah Metode Enzimatik Yayuk Kustiningsih; Nastiti Megawati; Jasmadi Joko Kartiko; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 3 No. 1 (2017): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Pre-analysis phase is the first step that determines the quality of the examination results, in this case, is the preparation of glucose work reagent. Glucose is one of the clinical examination clinical parameters that are often done in the laboratory. This glucose examination uses enzymatic methods. Enzyme activity is influenced by several factors, namely temperature, pH, substrate level, enzyme level, and inhibitor. Glucose examination performed at a temperature of 20-25oC or 37oC, but there are still laboratories in the field that is less attention to the initial temperature conditions of this reagent. This study aims to determine the effect of initial temperature variations of reagents on blood glucose levels of enzymatic methods. The research used Quasi Experimental research method with Posttest-Only Control Group Design design. The sample of this research used serum of a respondent who had no history of Diabetes Mellitus disease with 4 replication at each treatment. The results of the study with the initial temperature of 10oC, 13oC, 16oC, 19oC, 22oC and 25oC (control) respectively of 62.71 mg / dL; 65,74 mg / dL; 72.45 mg / dL; 76.91 mg / dL; 89.12 mg / dL and 97.19 mg / dL, the results of this study showed an increase in glucose levels in each treatment. Based on the results of linear regression analysis obtained a significance value of 0.000 with α = 0.05, which means Ho is rejected, so it can be concluded that there is an influence of initial temperature variation of reagent on blood glucose level. For the next researcher can do research about the effect of the initial temperature of reagent 22-38 oC on blood glucose enzymatic method. And the influence of incubation time on blood glucose level of enzymatic method.

Staphylococcus aureus and Methicillin resistant Staphylococcus aureus From The Diabetic Ulcer Leka Lutpiatina; Nisa Nur Agistni Eriana
Medical Laboratory Technology Journal Vol. 4 No. 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Infections of diabetic ulcers may occur because patients with diabetes mellitus have a weak immune system and the presence of high blood sugar into a strategic place for bacterial growth one of them is Staphylococcus aureus. Increased resistance of Staphylococcus aureus to various antibiotics, causing problems for the treatment of this infection. As by Methicillin-resistant Staphylococcus aureus (MRSA) This study aims to assess the presence of Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) at diabetic ulcer patient at Banjarmasin city hospital. This research uses descriptive observational research type. The study sample was patients with grade 2 and three diabetic ulcers with a total of 30 samples. The result of the research found 14 Staphylococcus aureus bacteria, Staphylococcus epidermidis nine, Staphylococcus saprophyticus two and 5 Gram-negative bacteria. Of the 14 samples of Staphylococcus aureus, there were eight samples of cefoxitin-resistant samples. The conclusion was 47% of Staphylococcus aureus in patients with a diabetic ulcer at Banjarmasin City Hospital. There was a 57% Methicillin-resistant Staphylococcus aureus in the isolated Staphylococcus aureus found. Need further research on the resistance of antibiotics oxacillin and cefoxitin to other Staphylococcus sp bacteria derived from patient diabetic ulcers.

Improved Mycology Media Formula of Corn and Cow’s Hair For Accelerate Superficial Mycosis Growth Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol. 7 No. 2 (2021): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Potato Dextrose Agar is growth and identification media of fungi that cause superficial mycoses, including Trichophyton rubrum and Trichophyton mentagrophytes with 10–14 days growth period. Modification media is needed to accelerate the growth of the fungus. Zea mays saccharata has a high carbohydrate, and cow’s hair contains high keratin, which can be used as a nutrient to replace fungal growth media. The study aimed to obtain a modified media formula containing Zea mays saccharata as a substitute for dextrose and cow's hair as a source of keratin to accelerate the growth of Trichophyton rubrum and Trichophyton mentagrophytes fungi. The modified media formula is mixing of Zea mays saccharata 15% to 25%, and cow's hair 20mg to 35mg. Acceleration of growth time based of when found microconidia and macroconidia, diameter measurements, and observations of texture and colonies color of Trichophyton rubrum and Trichophyton mentagrophytes. The study results are Zea mays saccharata media with the addition of cow hair for Trichophyton rubrum grew 11 days with 10%+20mg formula and grew only seven days with 25%+35mg formula. Modified media for Trichophyton mentagrophytes grew seven days for 10%+20mg formula, while it was only three days with 25%+35mg formula. The media formula with the fastest growth rate for Trichophyton rubrum and Trichophyton mentagrophytes was at a concentration of 25%+35mg. The Advanced Modified Media can become a patent medium for faster growth of superficial fungi at a lower cost. Further research needs to be done on how long this media can be stored for long-term use.

Sputum Quality of The Anytime And Outset for Examination Acid-Resistance Bacilli Leka Lutpiatina; Wahidah Wahidah; Nurhilaliah Nurhilaliah; Dinna Rakhmina; Rifqoh Rifqoh
Medical Laboratory Technology Journal Vol. 4 No. 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Diagnosis Tuberculosis (TB) can establish by microscopic examination of acid-resistant bacilli in the patient's sputum. Sputum quality greatly affects the diagnosis of TB, but the sputum collected by patients at community health centers sometimes does not meet the sputum quality requirements. The objective of this study was to assess the quality of sputum anytime and outset sputum on acid-resistant bacilli examination, from volume, color and viscosity parameters. The type of research used is descriptive observational. Respondents were suspected TB patients at the Marabahan and Aluh-aluh Public Health Centers of South Kalimantan province, Indonesia. Sputum samples taken were sputum anytime the first and outset sputum. The variables in this study are volume, color, and viscosity of sputum. The coloring of acid-resistant bacilli method Ziehl Nielsen. The results of the acid-resistant bacilli examination on sputum (86 specimens) found positive one (13%), positive three (2%), negative (85%). In morning sputum (86 specimens) found positive one (14%), positive three (2%), negative (84%). Sputum quality when with the good category as much as 36% and the quality of morning sputum with the good category as much as 55%. Conclusion Sputum outset has better quality than sputum at the anytime.

Produk Urikase dari Bacillus sp. Kontaminan Laboratorium leka lutpiatina
Medical Laboratory Technology Journal Vol. 1 No. 2 (2015): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Urease is an enzyme catalyst in the reaction of oxidation of uric acid into Allantoin. Oxidation of uric acid by the enzyme has become a basic principle of measurement of uric acid levels in the human body. This research to know urikase derived from the bacterium Bacillus sp that contaminate the air Laboratory of Microbiology Department of Health Analyst Banjarmasin. The research is descriptive survey. Airborne bacteria were isolated and identified for Bacillus sp. The identification is done by examination of macroscopic, microscopic and biochemical tests subsequently performed tests using nutrient agar medium containing 0.2% of uric acid. Results of the study showed there were two isolates of Bacillus sp and obtained clear zone on both these isolates. Conclusions of research there are contaminants Bacillus sp. as much as 40% in the laboratory. 100% of the yield urikase Bacillus sp. Urikase advice from Bacillus sp can be used as an alternative reagent uric acid probes spectophotometric method.

Antithrombotic Activity and Hemolysis Pattern of Fibrinolytic Protease-Producing Bacterial Isolates from the Coast of Tanjung Dewa, South Kalimantan Lutpiatina, Leka; Dwiyanti, Ratih Dewi; Insana, Aima; Rizal, Muhammad; Mufid, Muhammad
Medical Laboratory Technology Journal Vol. 11 No. 2 (2025): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v11i2.689

Cardiovascular disease is a leading cause of death worldwide, with thrombosis playing a key role in the pathological process. Conventional antithrombotic therapy often causes side effects, necessitating alternatives based on biological resources. Marine microorganisms are known to produce fibrinolytic protease enzymes that can degrade fibrin and prevent blood clot formation. This study aimed to evaluate the differences in antithrombotic activity and hemolysis patterns of fibrinolytic protease-producing bacterial isolates obtained from the coast of Tanjung Dewa Beach, South Kalimantan. A total of 15 isolates were obtained from seawater, beach sand, mollusks, crabs, and barnacles, and then subjected to colony morphology identification, Gram staining, and spore observation. Proteolytic activity testing using Skim Milk Agar media showed that all isolates had proteolytic activity with varying indices, with PSR1 showing the highest index (3.4). Furthermore, fibrinolytic testing using the fibrin plate assay method showed that 10 isolates were capable of degrading fibrin, with AL8 and SP2 showing the highest fibrinolytic indices (3.12 and 3.11, respectively). Antithrombotic testing using the clot lysis method revealed that AL7 and SP1 exhibited the highest lysis percentages (82.05% and 88.88%, respectively). Anticoagulant activity, as determined by the Lee-White method, showed that SP2 significantly prolonged the coagulation time (142 seconds, 49.65%). Hemolysis pattern testing revealed variations in activity, with AL8 and PSR1 classified as gamma-hemolytic and therefore potentially safer. Statistical analysis showed no significant differences between isolates in terms of antithrombotic activity, anticoagulant activity, or hemolysis pattern (p > 0.05). These findings suggest that coastal bacterial isolates from Tanjung Dewa Beach, particularly AL8 and SP2, have the potential to serve as safe, natural antithrombotic agents, supporting the development of biomedical therapies for cardiovascular disease.

Co-Authors Abdul Khair Abdul Khair Ahmad Muhlisin Ahmad Muhlisin Annisa Nur Yulianti Annisa Rahmi Anny Thuraidah Dinna Rakhmina Elfira Maulidah Erpan Roebiakto Fadia, Fadia Farah Indah Swari Gina Alia Mawaddah Habibah, Siti Noor hana nailah Helmiah, Tini Elyn Insana, Aima jasmadi Joko Kartiko Mufid, Muhammad Muhammad Ma'ruf MUHAMMAD RIZAL Nastiti Megawati Nisa Nur Agistni Eriana Noraida Noraida Noraida Noraida, Noraida Nur Rizqi Amaliah Nurhilaliah Nurhilaliah Nurlailah Nurlailah, Nurlailah Putri, Noorlaily Raimunah Raimunah Ratih Dewi Dwiyanti Ratih Dewi Dwiyanti, Ratih Dewi Rifqoh Rifqoh Sa'adah, Sa'adah Syaidatul Aslamiyah wahdah norsiah Wahidah Wahidah Widiyawati Widiyawati Yayuk Kustiningsih

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