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Teknik Kultur Embrio Jagung (Zea mays L.) melalui Modifikasi Media Kultur Jaringan Aziza, Elea Nur; Khoiriyah, Annisa; Rimartin, Geraldo Adinugra; Megawati, Sari
Jurnal Agroteknologi (Agronu) Vol 5 No 01 (2026): Jurnal Agroteknologi
Publisher : Universitas Ma'arif Nahdlatul Ulama Kebumen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53863/agronu.v5i01.2141

Abstract

This study explores the use of embryo culture as a strategic approach to rescue maize (Zea mays L.) embryos derived from interspecific crosses that fail to develop normally. Not all crosses result in viable seeds; in many cases, embryos remain immature or undergo developmental arrest, which increases the risk of embryo abortion before they can develop into complete plants. Embryo development failure in maize crosses, especially in combinations involving genetically distant genotypes, represents a major constraint in plant breeding programs. Without appropriate intervention, the resulting hybrid genotypes may be lost and consequently slow down the development of new superior varieties. To address this limitation, immature embryo culture represents an effective in vitro technique. Embryo culture involves the excision of young or incompletely developed plant embryos from seeds or ovaries and their subsequent cultivation on nutrient-rich artificial media to support further growth and plant regeneration. The objective of this study was to identify the optimal Murashige and Skoog (MS)-based culture medium composition for immature maize embryo growth using a factorial completely randomized design with three replications. Treatments consisted of combinations of agar concentration (4, 6, 8, and 10 g L⁻¹) and medium strength (0.5 MS, 0.75 MS, and 1 MS). The results indicated no significant interaction between MS medium strength and agar concentration. 1 MS medium produced the best results for shoot height, root length, and root number, whereas 0.75 MS medium resulted in the highest germination percentage. No significant differences were observed among treatments for days to germination and leaf number. An agar concentration of 6 g L⁻¹ yielded the highest germination percentage and shoot height, while other variables were not significantly affected by agar concentration. In conclusion, 1 MS medium or an agar concentration of 6 g L⁻¹ can be recommended for the culture of immature maize embryos
Evaluasi Pengaruh Teknologi Pruning pada Berbagai Usia Pertanaman terhadap Mutu Benih Padi (Oryza sativa L.) Khoiriyah, Annisa; Aziza, Elea Nur; Rimartin, Geraldo Adinugra
Jurnal Agroteknologi (Agronu) Vol 5 No 01 (2026): Jurnal Agroteknologi
Publisher : Universitas Ma'arif Nahdlatul Ulama Kebumen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53863/agronu.v5i01.2163

Abstract

This study aimed to analyze the effects of pruning at different crop ages on the physical, physiological, and biochemical quality of rice seeds, as well as to identify seed responses to variations in pruning timing. The experiment was conducted using a non-factorial Completely Randomized Design on the rice variety Inpari 32. Four pruning treatments were applied, namely no pruning (P0), pruning at 25 days after transplanting (DAT) (P1), 35 DAT (P2), and 45 DAT (P3), with three replications for each treatment. Observations focused on physical seed quality parameters, including moisture content, seed purity, and 1000-seed weight, as well as physiological seed quality parameters, namely germination percentage, vigor index, growth rate, rapid emergence (RE), percentage of normal seedlings, percentage of non-emerged fresh weight, and seed biochemical composition (total sugars, starch, and carbohydrates). Data were analyzed using analysis of variance (ANOVA) at a 5% significance level, followed by Duncan’s Multiple Range Test. The results revealed that pruning treatments did not significantly affect moisture content, seed purity, growth rate, germination percentage, vigor index, rapid emergence, percentage of abnormal seedlings, non-emerged fresh weight, starch content, or carbohydrate content. However, the highest 1000-seed weight was observed in the non-pruned treatment. In contrast, pruning at 35 DAT resulted in the highest total sugar content compared to other treatments, indicating its potential contribution to improved seed physiological quality. These findings suggest that pruning at an appropriate growth stage may influence specific seed quality attributes, particularly biochemical characteristics.
Technology-Based Utilisation of Liquid Organic Fertiliser Derived from Herbal Medicine Waste and Rabbit Urine to Enhance Turmeric (Curcuma longa L.) Growth Annisa Khoiriyah; Aziza, Elea Nur; Rimartin, Geraldo Adinugra
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 10 No. 1 (2026)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29165/ajarcde.v10i1.876

Abstract

The excessive use of synthetic fertilizers and pesticides in biopharmaceutical crop production has raised increasing concerns regarding environmental sustainability and food safety. This study investigated the potential of liquid organic fertilizer (LOF) derived from herbal medicine waste and rabbit urine as an alternative nutrient source for turmeric (Curcuma longa L.), focusing on plant growth, micronutrient uptake, and curcumin accumulation. A factorial randomized complete block design was implemented using varying concentrations of herbal medicine waste LOF (0–200 mL L?¹) and rabbit urine LOF (0–20 mL L?¹). Rhizome biomass, leaf iron (Fe) and boron (B) concentrations, and rhizome curcumin content were evaluated. The results demonstrated that moderate application rates of herbal medicine waste LOF (100 mL L?¹) combined with rabbit urine LOF (20 mL L?¹) effectively enhanced curcumin content without significantly increasing rhizome biomass. Elevated boron accumulation was observed at higher LOF concentrations. These findings highlight the potential of organic waste-derived LOF to improve turmeric quality while promoting sustainable and environmentally responsible cultivation practices. Contribution to Sustainable Development Goals (SDGs):SDG 2   : Zero Hunger SDG 12: Responsible Consumption and Production SDG 13: Climate ActionSDG 15: Life on Land