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Induksi Mutasi Kalus Embriogenik Gandum (Triticum aestivum L.) melalui Iradiasi Sinar Gamma untuk Toleransi Suhu Tinggi Ryan Budi Setiawan; Nurul Khumaida; Diny Dinarti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (438.095 KB) | DOI: 10.24831/jai.v43i1.9589

Abstract

Mutation techniques through gamma ray irradiation is useful to support breeding programs for genetic improvement of wheat. Genetic improvement on tolerance to high temperatures is necessary for development of wheat in Indonesia. The purpose of this study was to determine the level of radiosensitivity to be used as the basis for the induction of mutations by gamma ray irradiation on embryogenic callus to obtain putative mutants with high temperature tolerance. Explants used were embryogenic callus cultured on MS medium containing 30 g L-1 sukrosa, 2 g L-1 gelrite, 2 mg L-1 2.4D and 1 mg L-1 picloram. Culture incubated for 6 weeks with temperature 20±4 oC in the room culture. Five irradiation doses (10, 20, 30, 40 and 50 Gy) were used in radiosensitivity testing. A factorial, completely randomized design was applied to the experiment. The first factor was selection temperature with three levels (25, 30 and 35 oC), and the second factor was doses of gamma ray iradiation with three levels (10, 20, and 30 Gy). The result showed that radiosensitivity levels varied among varieties, LD20: 7.79 to 18.96 Gy and LD50: 24.29-33.63 Gy. Selayar variety which has highest sensitivity value compared with Dewata and Nias. Increasing doses of iradiation and temperature decrease survival of embryogenic callus, number of embryos, and percentage of germinated plantlets. Based on in vitro selection using high temperature (25, 30, and 35 oC), the obtained 19 putative mutants were derived from embryos that appear on the surface of embryogenic callus survival after irradiation and high temperature selection.Keywords: in vitro selection, putative mutant, radiosensitivity, somatic embryo
PENGARUH BEBERAPA KONSENTRASI Benzile Amino Purin (BAP) TERHADAP MULTIPLIKASI TUNAS NILAM (Pogostemon cablin Benth) Ryan Budi Setiawan; Dea Hayu Nastiti; Nanda Nur’aini; Ummi Hajra Dias; Teguh Saputra; Cecep Taupik Hidayat; Kelvin Rinaldi Siregar
Jurnal Sains Agro Vol 8, No 1 (2023): Jurnal Sains Agro
Publisher : Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/jsa.v8i1.1035

Abstract

Nilam (Pogostemon cablin Benth.) merupakan tanaman penghasil minyak atsiri potensial di Indonesia. Tingginya permintaan minyak nilam tidak diikuti oleh produksinya. Produktivitas nilam yang rendah salah satunya disebabkan oleh kurangnya ketersedian bibit bermutu. Salah satu metode alternatif perbanyakan bibit unggul dalam waktu relatif singkat dapat dilakukan melalui kultur jaringan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian beberapa konsentrasi zat pengatur tumbuh Benzile Amino Purin (BAP) terhadap multiplikasi tunas nilam secara in vitro. Penelitian telah dilaksanakan pada bulan Maret sampai Mei 2022 di Laboratorium Kultur Jaringan Departemen Agronomi, Fakultas Pertanian Universitas Andalas, Padang. Penelitian disusun berdasarkan rancangan acak lengkap dengan konsentrasi BAP sebagai perlakuannya yang terdiri dari empat taraf, yaitu 0 ppm; 0.5 ppm; 1 ppm dan 1.5 ppm pada media MS (Murashige dan Skoog). Hasil penelitian menunjukkan bahwa konsentrasi BAP sebanyak 0.5 ppm merupakan perlakuan terbaik yang mampu menghasilkan tunas sebanyak 134.6 tunas.
Demonstrasi Plot Pupuk Organik Cair (POC) Sabut Kelapa dan Batang Pisang di KWT Banda Langik Nalwida Rozen; Nurwanita Ekasari Putri; Aries Kusumawati; Ryan Budi Setiawan; Fitri Ekawati
Warta Pengabdian Andalas Vol 30 No 1 (2023)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.30.1.144-152.2023

Abstract

The community service took place for the Women Farming Group (WFG) of Banda Langik in the Sungai Bangek, Balai Gadang, Koto Tangah District, Padang City, from July to November 2022. It was held in demonstration plots (dump lots) of Liquid Organic fertilizers (LOF) with coconut coir, and banana stems as raw materials. These raw materials were straightforward to find in this area. Plots were made of 2 beds, and each was planted with pull-out Kale from seeds. Each bed was divided into two parts: without LOF and given LOF. It helped the WFG members differentiate the growth and yield pull-out Kale between being given LOF and without LOF. LOF was made by fermenting coconut coir separately and banana stems in a closed bucket for two weeks. The fermented products were sprinkled on the plants every week, starting at the age of two weeks after planting until they were close to harvest. The results showed that the plants given LOF were higher than those without LOF. The plant has given LOF more leaves. Their length and width of leaves were more significant than those without LOF. LOF is very useful given to vegetable plants, especially pull-out Kale.
Konservasi Ex Situ Kantong Semar (Nepenthes sumatrana (Miq) Beck) pada Beberapa Media Tanam Menggunakan Metode Split Anakan Ryan Budi Setiawan; Rizah Rizwana Wahyuni; Arif Kurniawan
JURNAL AGROTEKNOLOGI UNIVERSITAS ANDALAS Vol 1 No 1 (2017)
Publisher : Program Studi Agroteknologi Fakultas Pertanian Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jagur.1.1.45-49.2017

Abstract

Nepenthes is a unique plant in Indonesia whose existence is endangered and almost extinct, so that conservation efforts are required both in situ and ex situ. Vegetative propagation by separating tiller is one method that can be done, using a porous planting medium resam form of roots, husk, cocopeat and moss. The purpose of this study is to obtain the best media for breeding puppies Nepenthes (Nepenthes Sumatrana (Miq) Beck). The experiment is based on a randomized block design (RAK) to use the media's treatment plant that consists of 5 level and 3 experimental groups, the level of treatment trials include: M1 = root resam, M2 = husk, M3 = cocopeat, M4 = moss, M5 = combination of charcoal husk, cocopeat, resam roots and moss with a volume ratio of 1: 1: 1: 1. The observations are the following variables: plant height increment, increment the number of leaves, the length of leaves, leaf width increase, in the number of pitcher, pitcher the length and the length of roots. The results showed the best medium that can increase the number of leaves and number of pockets of most plant Nepenthes Sumatrana (Miq) Beck is the root media resam by increments of 4.55 strands and 2.77 pitcher. While the best medium that can enhance the long pitcher of Nepenthes sumatrana (Miq) Beck is a media combination with increasing 2.02 cm.
PAMERAN DISEMINASI HASIL RISET PADA PEKAN NASIONAL PETANI DAN NELAYAN (PENAS) XVI DI KOTA PADANG, SUMATERA BARAT Ryan Budi Setiawan; Indra Dwipa; Yaherwandi Yaherwandi; Reflinaldon Reflinaldon; Afrima Sari; Doni Hariandi; Juniarti Juniarti; Meisilva Erona S; Dede Suhendra; Awang Al Hamdi; Agil Syah Putra; Shinta Mulyana; Siti Rahmah; Nadila Aulia Ardi; Aries Novita Putri; Nandita Samaralya Tori
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 4 No. 3 (2023): Volume 4 Nomor 3 Tahun 2023
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v4i3.18422

Abstract

Hasil temuan suatu penelitian harus diketahui oleh masyarakat, baik secara spesifik dalam kelompok tertentu maupun kepada kalangan umum untuk menghasilkan dampak terhadap pembangunan. Diseminasi hasil riset dapat dilakukan melalui pameran pada kegiatan Pekan Nasional (PENAS) Petani Nelayan XVI. PENAS menjadi wadah tukar menukar informasi, pengalaman serta pengembangan kemitraan dan jejaring kerjasama antara para petani nelayan dan petani hutan, peneliti, penyuluh, pihak swasta dan pemerintah sehingga dapat membangkitkan semangat, tanggung jawab serta kemandirian sebagai pelaku utama pembangunan pertanian, perikanan dan kehutanan. Kegiatan PENAS dilaksanakan pada tanggal 10-15 Juni 2023 di Kota Padang, Sumatera Barat. Metode yang digunakan adalah pameran hasil penelitian, sosialisasi ketahanan pangan dan urban farming, diskusi dengan pengujung, pembagian benih dan leaflet gratis, serta pemasaran produk UKM hasil binaan dosen. Kegiatan pameran dan diseminasi hasil riset serta kerjasama pada PENAS ke XVI mendapat sambutan baik dan antusias yang tinggi oleh masyarakat dan peserta PENAS. Pengunjung mengetahui tentang hasil riset yang telah dilaksanakan oleh dosen, mendapatkan informasi tentang tanaman dan pertanian, memperoleh benih yang nanti akan ditanam untuk memenuhi kebutuhan rumah tangga.
The Efficacy Of Isopropyl Amine Glyphosate 165 Sl Herbicide Effect On Weed Control Of Coconut Cultivation Doni Hariandi; Ryan Budi Setiawan; Khairur Rizki
Jurnal Riset Perkebunan Vol. 4 No. 2 (2023): Jurnal Riset Perkebunan (JRP)
Publisher : Jurusan Budidaya Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrp.4.2.95-104.2023

Abstract

Coconut plants are an important commodity for Indonesian people. In the cultivation process, coconut plants need a suitable environment for growth and production. One of the problems in cultivation is weeds. Weeds have a negative impact on cultivated plants, therefore appropriate control measures are needed. So far, the most effective weed control is chemical methods using herbicides. One of the herbicides that can be used is the herbicide with isopropyl amine glyphosate 165 SL. The aim of the experiment was to determine the efficacy of the herbicide isopropyl amine glyphosate 165 SL for controlling weeds in coconut cultivation was conducted from February to June 2022 at Pariaman City, West Sumatra Province. The experimental units were laid out according to a Randomized Block Design with 7 treatments and 3 groups as replications. The treatment was herbicide isopropyl amine glyphosate 165 SL at doses of 3.50 l ha-1, 5.25 l ha-1, 7.00 l ha-1, 8.75 l ha-1, 10.00 l ha-1, manual weeding and control (without weeding). The results of the research show that (1) The herbicide isopropyl amine glyphosate 165 SL can generally control weeds in coconut cultivation up to 12 weeks after application because the weed biomass in the treatment plot is relatively the same as manual weeding and is lighter than the control; (2) Herbicide isopropyl amine glyphosate 165 SL with a dose range of 3.50 l/ha – 10.50 l ha-1 up to 6 weeks after application does not show symptoms of phytotoxicity in coconut plants.
Effect of the Combination of the Growth Regulators and Putrescine on the Somatic Embryogenesis of Wheat (Triticum Aestivum L.) on Some Types of Explants Yusniwati Yusniwati; Ryan Budi Setiawan
Journal of Applied Agricultural Science and Technology Vol. 7 No. 4 (2023): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v7i4.158

Abstract

Plant breeding programs need to be carried out in order to improve the genetics of wheat that is able to adapt to tropical environments through hybridization, mutation induction, tissue culture, and genetic transformation.  In vitro culture through somatic embryogenesis pathways plays an important role in genetic improvement and its integration with other breeding programs can positively affect the improvement of wheat quality, quantity, and development in Indonesia. The purpose of this study was to obtain an embryogenic callus induction method from the Dewata variety using five different types of explants, namely mature Seeds, immature embryos, immature seeds, leaf,  stem, and to obtain combination of plant growth regulators and putrescine on somatic embryogenesis of wheat.   The experimental design was prepared based on a complete randomized design with a combination treatment of embryogenic callus induction media consisting of 9 levels, namely: 1 ppm 2.4- D, 1 ppm 2.4-D + 1 ppm Picloram, ppm 2.4-D + 1 mg / L Picloram, 1 ppm 2.4-D + 10-4  M Putrescine, 1 ppm 2.4-D + 1 ppm Picloram +10-4  M Putrescine, 2 ppm 2.4-D + 1 ppm Picloram + 10-4  M Putrescine, 1 ppm 2.4 D + 10-3 M Putrescine, 1 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine, 2 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine. The results showed that the media used was able to induce embryogenic callus using mature seed and immature embryo, but  immature seed and leaf were not able to produce embryogenic callus. The best media  that produced the highest percentage of embryogenic callus was 2 ppm 2.4-D + 1 ppm Picloram + 10-4  M Putrescine with as much as 85.9% in young embryo explants.
SELEKSI IN VITRO KALUS KEDELAI (Glycine max L.) HASIL INDUKSI MUTASI MENGGUNAKAN ETIL METAN SULFONAT TOLERAN KEKERINGAN DAN ALUMINIUM Gustian Gustian; Muhammad Rafli Ibrahim; Benni Satria; Ryan Budi Setiawan; Silvia Permata Sari
Agros Journal of Agriculture Science Vol 26, No 2 (2024): Edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v26i2.4695

Abstract

Soybean (Glycine max L.) is a type of nutritious food crop because soybean seeds have high protein amino acid content. Increasing domestic production for soybean self-sufficiency needs to be done by expanding the planting area. The objective of this research is to examine the effect of ethyl methane sulfonate concentration and obtain soybean plants tolerant to drought and aluminum stress through in vitro selection. This research was conducted from November 2023 to January 2024 at the Tissue Culture Laboratory, Faculty of Agriculture, Andalas University. The research was arranged in a Completely Randomized Design consisting of 12 treatment levels of the combination of concentrations of abiotic selection agents PEG 4000 and AlCl3 induced by EMS mutation and 12 treatment levels of the combination of concentrations of abiotic selection agents PEG 4000 and AlCl3 not induced by EMS mutation with 3 replications. Data analysis was performed using independent sample t-test at a significance level of 5% and interpreted descriptively. The results showed that mutation induction using ethyl methane sulfonate (EMS) did not produce soybean somatic embryo mutants tolerant to drought and aluminum stress. Meanwhile, results from non-mutated callus produced somatic embryos suspected to be potential putative somaclonal candidates tolerant to drought and aluminum after double in vitro selection using 10% PEG 4000 and 150 ppm AlCl3 selection agents.
Expedition and Characterization of the Corpse Flower (Amorphophallus titanum (Becc.)) in West Sumatra Yusniwati, Yusniwati; Setiawan, Ryan Budi; Handayani, Mellyyana; Nanda, Alifma Rahimmi; Sukma, Dian; Rahmi, Alfazlul; Syahputra, Agil; Bosma, Pandu Arif Laksana; Baiturrahman, Atzim
Jurnal Manajemen Hutan Tropika Vol. 30 No. 2 (2024)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7226/jtfm.30.2.258

Abstract

The corpse flower (Amorphophallus titanum Becc.) is an endemic flora of Indonesia that is naturally found only in Sumatra. The status of A. titanum is endangered because of several factors: deforestation, tuber exploitation, long flowering time, and protogyne. Until now, there has been no research about the population and distribution of A. titanum in West Sumatra. This study aimed to obtain information about the population and distribution of A. titanum in West Sumatra, so this research is essential to learn. The research used roaming and snowball sampling methods in Solok Selatan and Sijunjung Regencies in September-October 2022. The research was conducted by recording the coordinates where A. titanum was discovered, marked using GPS, and collecting morphological characteristics. The results showed that exploration in the Solok Selatan District found 19 corpse flower individuals (18 species of A. titanum and one species of A. gigas). In contrast, exploration in Sijunjung Regency managed to found 25 species of A. titanum. The corpse flowers found were in vegetative, dormant, flowering, and fruiting phases. The results help record the number and distribution of A.titanum in West Sumatra. The ex-situ conservation program will use germplasm as propagation material in the future.
PENGARUH HERBISIDA ISOPROPIL AMINA GLIFOSAT 555 g/l TERHADAP PENGENDALIAN GULMA PADA TANAMAN KAKAO Hariandi, Doni; Setiawan, Ryan Budi
Agros Journal of Agriculture Science Vol 26, No 1 (2024): Edisi APRIL
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v26i1.4469

Abstract

Cocoa is a plant that is quite important for the national economy. However, in the production process there are several obstacles faced, such as the presence of weeds. Therefore, control measures need to be taken. An effective way to control weeds is to use herbicides. One of the herbicides that can be used is the herbicide isopropyl amine glyphosate 555 g/l. The aim of the experiment was to test the effect of the herbicide for weed control in cocoa cultivation. The experiment was carried out in Padang Pariaman Regency, West Sumatra Province from August to December 2020. The design used was a Randomized Block Design consisting of 7 treatments and 3 replications. Isopropyl amine glyphosate herbicide treatment 555 g/l with doses of 0.75, 1.00, 1.25, 1.50, 1.75 l/ha, manual weeding and control. The research results show that: (1) The herbicide isopropyl amine glyphosate 555 g/l can generally control weeds in cocoa cultivation for up to 12 weeks after application; (2) The effective dose to control weeds in cocoa cultivation is 0.75 – 1.50 l/ha because it has relatively the same results as manual weeding and a dose of 1.75 l/ha; (3) Isopropyl amine glyphosate herbicide 555 g/l with a dose range of 0.75 – 1.75 l/ha until observation 6 weeks after application did not show any symptoms of poisoning on cocoa plants. Keywords : cocoa, efficacy, herbicide, weed INTISARIKakao merupakan salah satu komoditi pertanian yang cukup penting bagi perekonomian nasional. Namun dalam proses produksinya terdapat beberapa kendala yang dihadapi seperti adanya gulma. Oleh karena itu, tindakan pengendalian perlu dilakukan. Cara efektif untuk mengendalikan gulma adalah dengan menggunakan herbisida. Salah satu herbisida yang dapat digunakan adalah herbisida isopropil amina glifosat 555 g/l. Tujuan percobaan adalah untuk menguji pengaruh herbisida terhadap pengendalian gulma pada budidaya kakao. Percobaan dilaksanakan di Kabupaten Padang Pariaman, Provinsi Sumatera Barat pada bulan Agustus sampai Desember 2020. Rancangan yang digunakan adalah Rancangan Acak Kelompok yang terdiri dari 7 perlakuan dan 3 ulangan. Perlakuan herbisida isopropil amina glifosat 555 g/l dengan dosis 0,75, 1,00, 1,25, 1,50, 1,75 l/ha, penyiangan manual dan kontrol. Hasil penelitian menunjukkan bahwa: (1) Herbisida isopropil amina glifosat 555 g/l secara umum dapat mengendalikan gulma pada budidaya kakao hingga 12 minggu setelah aplikasi; (2) Dosis efektif untuk mengendalikan gulma pada budidaya kakao adalah 0,75 – 1,50 l/ha karena mempunyai hasil yang relatif sama dengan penyiangan manual dan dosis 1,75 l/ha; (3) Herbisida isopropil amina glifosat 555 g/l dengan kisaran dosis 0,75 – 1,75 l/ha hingga pengamatan 6 minggu setelah aplikasi tidak menunjukkan gejala keracunan pada tanaman kakao. Kata Kunci: efikasi, gulma,  herbisida, kakao