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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Hortikultura Indonesia (JHI) Kultivasi Agrosains: Jurnal Penelitian Agronomi Jurnal Pertanian Agros Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Sains Agro Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Andalasian International Journal of Agricultural and Natural Sciences Jurnal Riset Perkebunan (JRP) Journal of Applied Agricultural Science and Technology JATIMAS : Jurnal Pertanian dan Pengabdian Masyarakat Jagur: Jurnal Agroteknologi Indonesian Journal of Forestry Research
Ryan Budi Setiawan
Universitas Andalas
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Medicine & Pharmacology Public Health Social Sciences Other

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Antioxidant activity (2-2-diphenil-1-picrilhydrazil radical-scavenging assay) and phytochemicals of Mimosa invisa Colla and Mimosa pigra L. grown at different terrestrial habitats. Chaniago, Irawati; Setiawan, Ryan Budi; Ardi, Ardi; Hariandi, Doni; Sari, Winda Purnama
Kultivasi Vol 24, No 1 (2025): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v24i1.62359

Abstract

Giant false sensitive plants (Mimosa invisa Colla) and giant sensitive plants (Mimosa pigra L.) are invasive plant species in the tropics and native to America. They are widely distributed in different ecosystems and soil types. Apart from being considered woody shrub weeds, these plant species can be used as herbal medicine for their antioxidant activities. An experiment to study the antioxidant activities and phytochemicals of M. invisa and M. pigra grown at 200 m from the coastline and close to the riverbank (riparian abandoned land) was carried out from March to November 2023. Weed leaves were collected as purposive randomized sampling from different terrestrial habitats in the City of Padang, West Sumatra. Antioxidant activity was identified according to a 2-2-diphenyl-1-picrilhydrazil (DPPH) radical-scavenging assay, and phytochemical compounds were identified qualitatively. Results demonstrate that M. invisa had mild antioxidant activity and M. pigra had strong antioxidant activity. The IC50 values for M. invisa grown at 200 m from the coastline and at the riverbank were 121.3 ± 11.5 and 105.6 ± 2.90 ppm, respectively. The IC50 values of M. pigra grown at 200 m from the coastline and at the riverbank were 80.6 ± 15.9 and 85.1 ± 9.03 ppm, respectively. Phenolic, saponin, and steroid were detected in all weeds grown at different habitats. However, alkaloids and terpenoids were not detected. Interestingly, flavonoid was not detected in the leaves of M. pigra grown at the riverbank.
Conservation Comprehensive Approach: Study on Exploration, Habitat Analysis, Propagation, and Reintroduction of the Indonesian Endemic Endangered Titan Arum (Amorphophallus titanum Becc.) Setiawan, Ryan Budi; Yusniwati, Yusniwati; Rahma Wati, Dela; Purnama Sari, Indah; Rahmah, Siti; Haryanti, Sindi; Arief Laksana Bosma, Pandu
Jurnal Manajemen Hutan Tropika Vol. 31 No. 3 (2025)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7226/jtfm.31.3.261

Abstract

Titan arum (Amorphophallus titanum Becc.) is an endemic species of Indonesia that is found naturally only in Sumatra and is classified as endangered, with an estimated 303 mature individuals remaining in their natural habitat. The population is decreasing due to deforestation, tuber exploitation, long flowering times, and protandry. Therefore, both in-situ and ex-situ conservation programs are necessary to preserve this species. The research aims to explore natural populations, analyze habitat conditions, develop propagation methods, and conduct reintroduction efforts to support the conservation of A. titanum. This study was conducted from 2023 to 2024 in Palupuah, Agam Regency, the Agriculture Faculty of Universitas Andalas, and several locations for the reintroduction of A. titanum. The research found that A. titanum habitat consists of forests rich in humus and litter, as well as banana cultivation areas. During the exploration, 7 individuals were identified: one in dormancy, one fruiting, and 5 in the vegetative phase. Seed germination experiments categorized seeds into seven classes based on their weight. The most dominant seed class weighed between 2.9 g and 3.2 g, comprising 38 seeds or 26.9% of the total. The germination rate and seedling growth were high, as all seeds exhibited 100% and produced seedlings of relatively uniform size. Propagation was carried out using in vitro culture techniques with petiole explants. The results showed that 2 mg L-1 benzyl aminopurine (BAP) successfully induced callus formation with a 100% induction rate, while a concentration of 1.5 mg L-1 achieved the highest shoot induction rate at 58.3%, with an average of 1.3 shoots and 7.9 roots per explant. The propagated seedlings were subsequently replanted in several locations for reintroduction activities.
Effect of the Combination of the Growth Regulators and Putrescine on the Somatic Embryogenesis of Wheat (Triticum Aestivum L.) on Some Types of Explants Yusniwati, Yusniwati; Setiawan, Ryan Budi
Journal of Applied Agricultural Science and Technology Vol. 7 No. 4 (2023): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v7i4.158

Abstract

Plant breeding programs need to be carried out in order to improve the genetics of wheat that is able to adapt to tropical environments through hybridization, mutation induction, tissue culture, and genetic transformation.  In vitro culture through somatic embryogenesis pathways plays an important role in genetic improvement and its integration with other breeding programs can positively affect the improvement of wheat quality, quantity, and development in Indonesia. The purpose of this study was to obtain an embryogenic callus induction method from the Dewata variety using five different types of explants, namely mature Seeds, immature embryos, immature seeds, leaf,  stem, and to obtain combination of plant growth regulators and putrescine on somatic embryogenesis of wheat.   The experimental design was prepared based on a complete randomized design with a combination treatment of embryogenic callus induction media consisting of 9 levels, namely: 1 ppm 2.4- D, 1 ppm 2.4-D + 1 ppm Picloram, ppm 2.4-D + 1 mg / L Picloram, 1 ppm 2.4-D + 10-4  M Putrescine, 1 ppm 2.4-D + 1 ppm Picloram +10-4  M Putrescine, 2 ppm 2.4-D + 1 ppm Picloram + 10-4  M Putrescine, 1 ppm 2.4 D + 10-3 M Putrescine, 1 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine, 2 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine. The results showed that the media used was able to induce embryogenic callus using mature seed and immature embryo, but  immature seed and leaf were not able to produce embryogenic callus. The best media  that produced the highest percentage of embryogenic callus was 2 ppm 2.4-D + 1 ppm Picloram + 10-4  M Putrescine with as much as 85.9% in young embryo explants.
Penggunaan Indole Butirat Acid (IBA) untuk Induksi Akar Setek Amorphophallus titanum dan Amorphophallus gigas Handayani, Mellyyana; Setiawan, Ryan Budi; Yusniwati; Jumsalia
Jurnal Hortikultura Indonesia (JHI) Vol. 14 No. 2 (2023): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.14.2.87-92

Abstract

Amorphophallus titanum dan Amorphophallus gigas merupakan flora endemik Sumatera yang terancam punah. Perbanyakan tanaman melalui setek dapat digunakan untuk menunjang kegiatan konservasi. Keberhasilan setek ditentukan oleh konsentrasi zat pengatur tumbuh (ZPT) untuk menginduksi terbentuknya akar, corm dan tunas, sehingga kajian tentang konsentrasi ZPT penting untuk dipelajari. Penelitian ini bertujuan untuk mendapatkan konsentrasi IBA terbaik untuk menginduksi akar dan corm pada A. titanum dan A. gigas. Penelitian telah dilakukan dari bulan Juli-Oktober 2022. Penelitian disusun berdasarkan rancangan acak lengkap dengan perlakuan konsentrasi IBA yang terdiri dari 5 taraf yaitu: 5, 10, 15, 20 dan 25 mg L-1. Setek pada A. titanum menggunakan rachis dan petiole, sedangkan pada A. gigas hanya menggunakan rachis. Hasil penelitian menunjukkan bahwa setek petiole menunjukkan respons yang lebih baik dibandingkan dengan rachis dalam menginduksi terbentuknya akar pada A. titanum. Konsentrasi IBA 15 mg L-1 menghasilkan persentase berakar sebesar 80% pada setek petiole A. titanum dan 20% pada setek rachis A. gigas.Kata Kunci : biodiversitas, endemik, konservasi, punah, zat pengatur tumbuh
Penggunaan Indole Butirat Acid untuk Setek Pucuk Kantong Semar (Nepenthes ampullaria Jack.) Hijau Polos: Application of Indole Butirat Acid to Cuttings of Plain Green Pitcher Plant (Nepenthes ampullaria Jack.) Handayani, Mellyyana; Setiawan, Ryan Budi; Jumsalia
Jurnal Hortikultura Indonesia (JHI) Vol. 15 No. 3 (2024): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.15.3.155-162

Abstract

Kantong semar (Nepenthes ampullaria Jack.) merupakan spesies endemik yang tersebar di Sumatera dan Kalimantan. Spesies ini berstatus least concern karena minimnya data penelitian terbaru mengenai kelimpahan dan penyebarannya. Eksploitasi untuk perdagangan dan deforestasi menyebabkan penurunan populasi. Selain itu, waktu yang lama untuk mencapai fase generatif dan tipe pembungaan dioecious juga berkontribusi terhadap penurunan populasi Nepenthes sp. Program konservasi baik in situ maupun ex situ sangat penting untuk menjaga kelestarian N. ampullaria. Perbanyakan tanaman melalui setek dapat mendukung kegiatan konservasi. Keberhasilan setek ditentukan oleh konsentrasi zat pengatur tumbuh (ZPT) untuk menginduksi pembentukan akar dan tunas, sehingga kajian tentang konsentrasi ZPT penting untuk dipelajari. Penelitian ini bertujuan untuk menentukan konsentrasi IBA terbaik untuk menginduksi akar dan tunas pada N. ampullaria. Penelitian disusun berdasarkan rancangan acak lengkap dengan perlakuan konsentrasi IBA yang terdiri dari 5 taraf yaitu: 0, 5, 10, 15, dan 20 mg L-1. Hasil penelitian menunjukkan bahwa konsentrasi IBA tidak berpengaruh nyata terhadap pertumbuhan stek pucuk N. ampularia dengan persentase hidup setek berkisar 70-100 %, pertambahan tinggi tunas berkisar 5.01-9.53 cm, jumlah akar primer berkisar 5.89-9.56 helai dan jumlah akar primer berkisar 3.52-6.29 cm. Semua perlakuan mampu meransang terbentuknya akar dan tunas yang mengindikasikan bahwa kandungan fitohormon endogen telah mencukupi untuk pertumbuhan setek pucuk pada N. ampullaria. Kata kunci: biodiversitas, endemik, konservasi, punah, zat pengatur tumbuh
Proliferasi Kalus Embriogenik dan Embrio Somatik Tanaman Gandum (Triticum aestivum L.) Setiawan, Ryan Budi; Khumaida, Nurul; Dinarti, Diny
Jurnal Sains Agro Vol 9, No 1 (2024): Jurnal Sains Agro
Publisher : Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/jsa.v9i1.1361

Abstract

Dalam rangka mengurangi tingginya nilai impor gandum perlu dilakukan upaya penanaman dengan tetap memperhatikan faktor kesesuaian lingkungan. Program pemuliaan tanaman penting dilakukan untuk perbaikan genetik dan karakter sehingga berpengaruh positif terhadap peningkatan kualitas, kuantitas dan pengembangan gandum di Indonesia. Embriogenesis somatik memainkan peranan yang sangat besar dalam perbanyakan tanaman. Integrasi dengan program pemuliaan konvensional maupun teknik molekular/bioteknologi memungkinkan embriogenesis somatik menjadi suatu alat untuk meningkatan laju perbaikan genetik tanaman. Tujuan penelitian ini adalah mendapatkan metode proliferasi kalus embriogenik dan embrio somatik tanaman gandum. Media terbaik untuk proliferasi kalus embriogenik pada varietas Dewata dan Nias adalah 1.0  mg L-1 2.4D + 1.0 mg L-1 picloram dan pada varietas Selayar adalah 1.0  mg L-1 2.4D. Aplikasi media cair pada proliferasi embrio somatik menunjukkan hasil tertinggi sebanyak 28.88 embrio pada Dewata dan 15.13 embrio pada Nias.
The Efficacy Of Isopropyl Amine Glyphosate 165 Sl Herbicide Effect On Weed Control Of Coconut Cultivation Hariandi, Doni; Setiawan, Ryan Budi; Rizki, Khairur
Jurnal Riset Perkebunan Vol. 4 No. 2 (2023): Jurnal Riset Perkebunan (JRP)
Publisher : Jurusan Budidaya Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrp.4.2.95-104.2023

Abstract

Coconut plants are an important commodity for Indonesian people. In the cultivation process, coconut plants need a suitable environment for growth and production. One of the problems in cultivation is weeds. Weeds have a negative impact on cultivated plants, therefore appropriate control measures are needed. So far, the most effective weed control is chemical methods using herbicides. One of the herbicides that can be used is the herbicide with isopropyl amine glyphosate 165 SL. The aim of the experiment was to determine the efficacy of the herbicide isopropyl amine glyphosate 165 SL for controlling weeds in coconut cultivation was conducted from February to June 2022 at Pariaman City, West Sumatra Province. The experimental units were laid out according to a Randomized Block Design with 7 treatments and 3 groups as replications. The treatment was herbicide isopropyl amine glyphosate 165 SL at doses of 3.50 l ha-1, 5.25 l ha-1, 7.00 l ha-1, 8.75 l ha-1, 10.00 l ha-1, manual weeding and control (without weeding). The results of the research show that (1) The herbicide isopropyl amine glyphosate 165 SL can generally control weeds in coconut cultivation up to 12 weeks after application because the weed biomass in the treatment plot is relatively the same as manual weeding and is lighter than the control; (2) Herbicide isopropyl amine glyphosate 165 SL with a dose range of 3.50 l/ha – 10.50 l ha-1 up to 6 weeks after application does not show symptoms of phytotoxicity in coconut plants.
Induksi Mutasi Kalus Embriogenik Gandum (Triticum aestivum L.) melalui Iradiasi Sinar Gamma untuk Toleransi Suhu Tinggi Setiawan, Ryan Budi; Khumaida, Nurul; Dinarti, Diny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 43 No. 1 (2015): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (438.095 KB) | DOI: 10.24831/jai.v43i1.9589

Abstract

Mutation techniques through gamma ray irradiation is useful to support breeding programs for genetic improvement of wheat. Genetic improvement on tolerance to high temperatures is necessary for development of wheat in Indonesia. The purpose of this study was to determine the level of radiosensitivity to be used as the basis for the induction of mutations by gamma ray irradiation on embryogenic callus to obtain putative mutants with high temperature tolerance. Explants used were embryogenic callus cultured on MS medium containing 30 g L-1 sukrosa, 2 g L-1 gelrite, 2 mg L-1 2.4D and 1 mg L-1 picloram. Culture incubated for 6 weeks with temperature 20±4 oC in the room culture. Five irradiation doses (10, 20, 30, 40 and 50 Gy) were used in radiosensitivity testing. A factorial, completely randomized design was applied to the experiment. The first factor was selection temperature with three levels (25, 30 and 35 oC), and the second factor was doses of gamma ray iradiation with three levels (10, 20, and 30 Gy). The result showed that radiosensitivity levels varied among varieties, LD20: 7.79 to 18.96 Gy and LD50: 24.29-33.63 Gy. Selayar variety which has highest sensitivity value compared with Dewata and Nias. Increasing doses of iradiation and temperature decrease survival of embryogenic callus, number of embryos, and percentage of germinated plantlets. Based on in vitro selection using high temperature (25, 30, and 35 oC), the obtained 19 putative mutants were derived from embryos that appear on the surface of embryogenic callus survival after irradiation and high temperature selection.Keywords: in vitro selection, putative mutant, radiosensitivity, somatic embryo
Diversity of orchids (Orchidaceae) and host trees at Padang, West Sumatra, Indonesia: A preliminary step towards germplasm conservation Setiawan, Ryan Budi; Handayani, Mellyyana; Rahimmi Nanda, Alifma; Sukma, Dian; Rahmi, Alfazlul; Syahputra, Agil
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 2 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i2.56159

Abstract

Orchid exploration brings numerous benefits, including the conservation of rare species, ecological insights, medicinal and horticultural applications, economic opportunities, cultural appreciation, and scientific advancement. These benefits underscore the importance of continued exploration and conservation efforts to safeguard orchid diversity. This study aimed to explore the diversity of orchids at Universitas Andalas, Padang, West Sumatra. The research involved a comprehensive survey of orchid species in various habitats, documenting their distribution and abundance. We used a systematic sampling method to collect plant specimens and record relevant ecological data. The research indicated a remarkable diversity of orchid species at Universitas Andalas. We found 21 species of orchids, including 19 epiphytes and two terrestrial species. Filicium desipiens was the most dominant host, with 13 species of orchids found on 15 trees. Dendrobium crumenatum was the most common orchid found on 13 host trees. The results highlighted the importance of preserving these unique orchid populations. This research contributes to the understanding of orchid diversity at Universitas Andalas. The findings can inform policymakers, conservationists, and local communities in developing effective strategies for the preservation and sustainable use of orchid resources. Future studies should focus on further exploration, taxonomic studies, and conservation initiatives to ensure long-term survival. Keywords: conservation; diversity; epiphytes; exploration; terrestrial
IBA-induced rooting in Nepenthes reinwardtiana Miq. cuttings for germplasm conservation Setiawan, Ryan Budi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 1 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i1.60254

Abstract

Nepenthes reinwardtiana Miq. is an endemic species distributed in Sumatra and Kalimantan and classified as least concern due to the lack of recent research data on its abundance and distribution. Excessive exploitation, deforestation, the long time required to reach the generative phase, and the dioecious flowering type have caused a population decline. Plant propagation through cuttings can support conservation activities. The success of cuttings is determined by the concentration of plant growth regulators (PGR), making studies on PGR concentration important. This research aimed to determine the best indole-3-butyric acid (IBA) concentration for inducing roots and shoots in N. reinwardtiana. The study was conducted from February to October 2023. The research was arranged based on a completely randomized design with IBA concentration treatments consisting of 5 levels: 0, 5, 10, 15, and 20 mg L-1. The results showed that there was no significant effect of IBA on all variables. However, 5 mg L-1 IBA gave the best responses on shoot height increase (10.9 cm), leaf number increase (5.0 leaves), and root length (8.7 cm). These findings suggest that the growth of N. reinwardtiana cuttings may rely heavily on endogenous auxin levels, reducing the observable effects of exogenous IBA application. The non-significant results highlight the potential of this species to thrive without external auxin under certain conditions, which could simplify propagation protocols. Keywords: biodiversity; conservation; endemic species; extinct; plant growth regulator
Co-Authors . Reflinaldon Adhira Prameswari Ranayudha Afrima Sari Agil Syah Putra Aprizal Zainal Ardi Ardi Arief Laksana Bosma, Pandu Aries Kusumawati Aries Novita Putri ARIF KURNIAWAN Awang Al Hamdi Baiturrahman, Atzim Benni Satria Bosma, Pandu Arif Laksana Cecep Taupik Hidayat Dea Hayu Nastiti Dian Sukma, Dian Diny Dinarti Doni Hariandi Ekasari Putri, Nurwanita ekawati, fitri G Gustian Gustian Gustian Handayani, Mellyyana Haryanti, Sindi Hayati, PK Dewi I Imelinda Indra Dwipa Irawati Chaniago Jumsalia Juniarti Juniarti Kelvin Rinaldi Siregar Khairur Rizki Meisilva Erona Sitepu Muhammad Rafli Ibrahim Nadila Aulia Ardi Nalwida Rozen Nanda Nur’aini Nanda, Alifma Rahimmi Nandita Samaralya Tori Nurul Khumaida Purnama Sari, Indah Putri Salsa Afifah Rahimmi Nanda, Alifma Rahma Wati, Dela Rahmi, Alfazlul Rizah Rizwana Wahyuni Rizki, Khairur Sari, Winda Purnama Sembiring, Rinawati Shinta Mulyana Silvia Permata Sari Siti Rahmah Syahputra, Agil Teguh Saputra Ummi Hajra Dias Yaherwandi Yusniwati Yusniwati Yusniwati Zulfadly Syarif