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IDENTIFIKASI SENYAWA ANTIMIKROBA EKSTRAK MIKROALGA LAUT TETRASELMIS CHUII (KAJIAN METODE EKSTRAKSI MASERASI, JENIS PELARUT, DAN WAKTU EKSTRAKSI) Maligan, Jaya Mahar; Widayanti, Vindhya Tri; Zubaidah, Elok
Jurnal Teknologi Pertanian Vol 16, No 3 (2015)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1162.386 KB)

Abstract

Tetraselmis chuii dikenal sebagai mikroorganisme photoautotroph yang mampu menghasilkan senyawa antimikroba sebagai metabolisme atau senyawa alelopati. Berkenaan dengan metabolit yang berharga, penelitian ini dilakukan untuk mendapatkan informasi tentang ekstraksi dan identifikasi senyawa antimikroba oleh T. chuii menggunakan metode maserasi. Pada penelitian, rancangan acak dikerjakan untuk mengekstrak dan mengidentifikasi senyawa antimikroba oleh T. chuii. Dua faktor dan tiga tingkat yang diperlukan dalam penelitian ini. Faktor pertama adalah jenis pelarut (metanol (L1), kloroform (L2), dan aseton (L3)), kedua adalah waktu ekstraksi (24 (T1), 48 (T2), dan 72 (T3) jam). Hasil penelitian menunjukkan bahwa interaksi antara jenis pelarut dan waktu ekstraksi memberikan pengaruh yang signifikan (P<0.05) pada ekstrak hasil senyawa antimikroba. Hasil tertinggi dan terendah ekstrak senyawa antimikroba yang diperoleh di Runs L3T3 dan L2T1 sekitar, 29.92 dan 2.01%. Hasil menunjukkan bahwa diameter tertinggi zona bening diperoleh di Run L2T3, sekitar 14.30; 16.30; 13.33; 14.33 mm E. coli, S. aureus, C. albicans, dan A. flavus. GC-MS digunakan sebagai alat bantu analisa senyawa antimik-roba T.chuii yang meliputi asam lemak (asam 9-hexadecanoic, asam heksadekanoat (asam palmitat), asam 9-octadecenoic), alcane (Docosane, Tricosane, Eicosane, Nonadecena), cycloalcene (Cyclohexene), senyawa ester (asam heksadekanoat (etil ester), asam 1,2-benzenedicarboxylic), senyawa alkohol (Benzil alkohol), dan di-terpenoide (fitol dan 2,6,10 trimetil). Oleh karena itu, didapatkan hasil bahwa perlakuan terbaik dicapai ketika kloroform digunakan sebagai pelarut dengan waktu ekstraksi 72 jam (L2T3) dan menyebabkan diameter zona bening.
Manajemen Budidaya Lebah Madu Klanceng (Trigona sp.) di Kelompok Tani Hutan (KTH) Telaga Lestari, Desa Ngebel, Kab. Ponorogo, Jawa Timur Al Awwally, Khotibul Umam; Mustaniroh, Siti Asmaul; Andriani, Ria Dewi; Yulianingsih, Rini; Widayanti, Vindhya Tri; Hermawan, Rizky; Sunyoto, Nimas Mayang Sabrina; Sundari, Shafira Arini
Journal of Innovation and Applied Technology Vol 9, No 2 (2023)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiat.2023.9.2.12

Abstract

Lebah klanceng (Trigona sp) merupakan lebah yang tidak bersengat dan berasal dari negara yang memiliki kawasan iklim tropis maupun subtropics.  Budidaya lebah klanceng memiliki prospek tang tinggi dan dapat menjadi ekonomi alternative untuk masyarakat di Desa Ngebel. Tujuan kegiatan ini adalah untuk mengetahui manajemen pemeliharaan lebah madu Trigona sp mulai dari pengadaan koloni, produksi, pakan dan pengolahan madu. Dalam pembudayaan lebah madu Trigona sp beberapa yang perlu disiapkan adalah lokasi budidaya, setup untuk budidaya, koloni, pakaian kerja, dan peralatan lain yang diperlukan. Metode yang digunakan pada kegiatan ini adalah observasi, partisipasi, dan wawancara dengan peternak lebah madu klanceng di wilayah KTH Telaga Sari. Dari hasil kegiatan didapatkan bahwa manajemen budidaya lebah madu klanceng harus ditingkatkan untuk meningkatkan kualitas madu klanceng yang dihasilkan, yang dapat membawa Desa Ngebel sebagai salah satu sentra produksi madu klanceng di Indonesia.
Effect of concentration of sugar and dried Dayak onion (Eleutherine palmifolia) on the quality of Dayak onion kombucha Permatasari, Vitta Rizky; Widayanti, Vindhya Tri; Falasifah, Ratu; Sunyoto, Nimas Mayang Sabrina; Suhartini, Sri
Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Vol 7, No 3 (2024)
Publisher : Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.afssaae.2024.007.03.2

Abstract

Kombucha is one example of a health drink product that falls under the category of functional drinks. Dayak onions are one of the ingredients with many bioactive components. This study aimed to determine the optimal combination of treatments to yield the highest quality Dayak onion kombucha by assessing the effects of variations in sugar concentration and dried Dayak onion concentration on the quality of Dayak onion kombucha (i.e., pH, total acid, and organoleptic). The study employed a randomized block design (RBD) with three replications to produce 27 experimental units. The two factors were dried Dayak onion concentrations of 2%, 4%, and 6% (w/v) and sugar concentrations of 5%, 7.5%, and 10% (w/v). The data was then subjected to a two-way ANOVA using IBM SPSS Statistics 26 software, and the Multiple Attribute method was used to determine the best treatment. The results demonstrated that the concentration of sugar and dried Dayak onions had a significant effect (α<0.05) on the pH value, total acid.  the higher the concentration of sugar, the higher the total acid content with a lower pH, and the higher the concentration of dried Dayak onions, the lower the total pH. The best treatment combination was using dried Dayak onion concentration of 6% (w/v) and sugar concentration of 7.5% (w/v). The test results obtained were pH 3.173, total acid 0.105%, organoleptic color 3.867, and organoleptic aroma 2.867.
Analysis of the pretreatment process of gonggong shell flour (Laevistrombus turturella) on a scale up Diyah Dewanti, Beauty Suestining; Rumandhani, Nuvia Nurlaily; Febrianto, Arie; Widayanti, Vindhya Tri; Rucitra, Andan Linggar; Rohmah, Wendra Gandhatyasri; Septifani, Riska; Ikasari, Dhita Morita
Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Vol 8, No 1 (2025)
Publisher : Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.afssaae.2025.008.01.8

Abstract

The gonggong shell (Laevistrombus turturella) is a species of sea snail found in the waters surrounding Bintan, Riau Islands. The gonggong consists of both meat and shell, with the shell constituting the largest portion, approximately 60%. The high proportion of shells materials compared to  the meat results in abundant gonggong shell waste. One way to utilize gonggong shells is by converting the shells into shell flour, which can be used in health supplements and as feed for shrimp, particularly in shrimp farms in Bintan Regency. The hard texture of gonggong shells (13.1 MPa) requires pretreatment before milling. The pretreatment processes include autoclaving, drying, and burning. This study aimed to assess the quality difference between the scaled-up  and laboratory-scale processes, and to identify the material requirements and utilities for the large-scale pretreatment process of gonggong shell to produce shell flour. The scale-up was conducted with a 1 : 50 ratio from the laboratory scale. The quality results of gonggong shell flour in the large-scale study showed a moisture content of 0.46%, an ash content of 56.42%, and a calcium content of 54.31%. These results were compared with those obtained at the laboratory scale using an unpaired t-test, and no significant differences were found for all tested parameters.
Physicochemical properties of yogurt synbiotic enriched with bawang Dayak (Eleutherine palmifolia (L) Merr) extract Andriani, Ria Dewi; Sawitri, Manik Eirry; Widayanti, Vindhya Tri; Manab, Abdul; Rahayu, Premy Puspitawati; Prasasti, Finna; Saleha, Rischa Amalia
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 2 (2024): August 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.02.1

Abstract

Yogurt is a synbiotic yogurt made from a combination of probiotics and prebiotics, so the addition of bawang dayak extract (Eleutherine palmifolia (L) Merr) improved the functional properties of the synbiotic yogurt. The research objective was to analyse the physicochemical properties of yogurt synbiotic enriched with bawang dayak extract. The physicochemical properties, pH, acidity, syneresis and water holding capacity were analysed. This research used a completely randomized design (CRD), which consisted of 4 treatments and 3 replications with various concentrations of bawang dayak extract (T0 = 0%, T1 = 1%, T2 = 4% and T3 = 6%). The main ingredients used were skim milk, fresh milk, yogurt starter (Lactobacillus bulgaricus, Streptococcus thermophilus) and bawang dayak extract. The results of this study showed that the pH, acidity and water holding capacity were not significantly different (p>0.05) among the treatments, while syneresis was highly significantly different (p<0.01) at the T3 (6%) concentration.