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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Natur Indonesia Jurnal Agrista Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Prosiding Seminar Biologi Jurnal Sumberdaya Hayati BERITA BIOLOGI Jurnal Penelitian Sains Jurnal Penelitian Pertanian Tanaman Pangan Menara Perkebunan Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik) Makara Journal of Science Jurnal Kedokteran Hewan Journal Of Biology Education Research (JBER)

Lisdar Idwan Sudirman

Department Of Biology, Faculty Of Mathematics And Natural Science, Bogor Agricultural University, IPB Dramaga Campus, Bogor, West Java

Author-ID : 624776

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Komunitas Mikrofungi pada Lapisan Horizon Serasah Acacia mangium Samingan Samingan; Lisdar I. Sudirman; Dede Setiadi; Alex Hartana; Budi Tjahjono
Jurnal Agrista Vol 12, No 2 (2008): Volume 12 Nomor 2 Agustus 2008
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1003.952 KB)

Microfungal Community on Litter Horizon Layer of Acacia mangiumABSTRACT. Fungal diversity on litter horizon layer of Acacia mangium were investigated to examined fungal species and fungal community on each litter horizon layer, and also to examined relationship between organic content of litter and fungal community. Twenty two species were isolated from three litter horizon layer with dilution method. Total fungal population on five years old A. mangium standing was higher than two years old, whereas on logging former area was low. Total fungal population on standing two and five years old were highest on L layer follow by F and H layer, but on logging former area were highest F layer followed by L and H layer. Aspergillus was dominate on H layer in almost of sampling collection area, beside that Aspergillus and Penicillium were found also on L and F layer. Generally L and F layer dominated by Sp7, Sp5, Sp20, and Sp22. The highest diversity indice on two years old standing was found at different layer.; L and H on health and Genoderma attacked standing, whereas on standing five years old, highest diversity indices was found at L layer, but on logging former area highest diversity indices was found at H layer.

Peran Makhluk Tersembunyi dan Terabaikan bagi Kesehatan dan Lingkungan Lisdar I Sudirman
Prosiding Seminar Biologi Vol 1 No 1 (2015): Prosiding Seminar Nasional Mikrobiologi Kesehatan dan Lingkungan
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v1i1.2100

Cendawan yang termasuk dalam fungal kingdom adalah organisme unik dan bukan termasuk dalam dunia tumbuhan. Cendawan dapat bersifat mikroskopis dan makroskopis (makrofungi, jamur) yang dapat dilihat oleh mata telanjang. Cendawan mempunyai kemampuan berasosiasi dengan organisme lain seperti dengan ganggang dan atau sianobakteri sehingga terbentuk organisme lain yang dinamakan liken. Makrofungi dan liken mempunyai peran menguntungkan bagi kesehatan dan lingkungan. Daerah tropis, termasuk Indonesia kaya akan keragaman ke dua organisme tersebut tetapi kurang dieksplorasi dan diteliti sehingga pemanfaatannya jauh tertinggal dari negara di dunia, bahkan dengan negara di Asean. Pada makalah ini akan dipaparkan potensi makrofungi atau jamur terutama jamur pelapuk kayu putih (white rot fungi) dan liken dalam bidang kesehatan dan lingkungan dalam mengatasi polutan.

Karakterisasi α-Amilase dari Aspergillus versicolor 3a1 yang Diproduksi pada Media Limbah Cair Tapioka Fitratul Aini; Nisa Rachmania Mubarik; Lisdar A. Manaf
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2581

The aim of this experiment was to characterize A. versicolor 3a1 α-amylase produced on cassava liquid waste media. Two types of media, base and combination media, were used as a comparison. Cassava liquid waste media contains 1% cassava starch, 1% yeast extract, 0.13% KH2PO4, and 0.05% MgSO4 diluted in cassava liquid waste. Base media contains same composition but using aquadest as a solvent, and combination media using mixture of aquadest and cassava liquid waste. A. versicolor 3a1 α-amylase showed its maximum specific activity in cassava liquid waste, base, and combination media after 3, 7, and 4 days incubation, respectively. Crude extract of α-amylase fromA. versicolor 3a1 was precipitated in 20-80% (w/v) ammonium sulphate. Precipitation of A. versicolor 3a1 α-amylase with 70% (w/v) ammonium sulphate on cassava liquidwaste, 60% on base media, and 60% on combination media will increase its specific activity 16.6, 4.28, and 5.65 times, respectively, compared to the specific activities ofcrude before precipitation. α-Amylase crude extract from A. versicolor 3a1 from all media showed its highest specific activity at 70oC and pH 5.0, and addition of FeSO4 increased the specific activity. Precipitated A. versicolor 3a1 α-amylase from all media showed its highest specific activity at 70oC and pH 6.0. Addition of FeSO4 precipitated 3a1 α-amylase from base and combination media will increase its specific activity, while MgSO4 will increase its specific activity in cassava liquid waste media. Thermostability assay revealed that the crude and the precipitated 3a1 α-amylase were relatively stable at 70oC up to 180 minutes incubation, except for precipitated3a1 -amylase on cassava waste media. Crude α-amylase 3a1 was relatively stable at pH 5-9 up to 1 hour incubation with wide pH ranges, while the precipitated with narrow pH ranges.

Pertumbuhan dan Produksi Jamur Lentinus sajor-caju isolat LSC9 pada Media Serbuk Gergajian Kayu Sengon (Paraserianthes falcataria) dan Tandan Kosong Kelapa Sawit HENNY SULISTANY; LISDAR IDWAN SUDIRMAN
Jurnal Sumberdaya Hayati Vol. 1 No. 2 (2015)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.1.2.41-46

Paraserianthes falcataria sawdust (SGS) and oil palm empty fruit bunch (TKKS) are by-product of forestry and oil palm industries. SGS is commonly substrates for mushroom cultivation. TKKS is expected to be an alternative substrates for mushroom cultivation besides SGS. This study was conducted to determine the growth and fruiting body production of Lentinus sajor-caju LSC9 isolate on SGS, TKKS and mixtures of both substrates (C) with proportion 1:1 respectively. Each substrates were added with 15% rice bran, 1.5% gypsum and 1.5% CaCO3 with a total weight of 500 g/bag. The result showed that the growth and fruiting body production of Lentinus sajor-caju LSC9 isolate on SGS was better than TKKS and C substrates with biological efficiency on SGS substrates (50.88%) higher than TKKS substrates (34.42%) and C substrates (29.51%), with vegetative phase (16 days), generative phase (100 days) and growth and development phase (115 days) on TKKS substrates were shorter than SGS and C substrates. The greatest pileus number found on SGS substrates (12 pieces), while the largest pileus diameter found on C substrates (10.17 cm). Nevertheless, TKKS can be used as alternative substrates for fruiting body production of Lentinus sajor-caju LSC9 isolate.

PATOGENISITAS CENDAWAN Lecanicillium sp. PTN01 TERHADAP PENGGEREK TONGKOL JAGUNG Helicoverpa armigera (HUBNER) (LEPIDOPTERA: NOCTUIDAE) Sempurna Ginting; Teguh Santoso; Yayi Munara; Ruly Anwar; Lisdar Sudirman
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i1.3378

Helicoverpa armigera is one of the most important agricultural pests because it has a high reproductive rate and resulted in economic losses. One of the H. armigera control techniques that is in accord with IPM principle is the utilizing of entomopathogenic fungus. The aim of this research was to test the pathogenicity of Lecanicillium (Lecanicillium sp. PTN01) against H. armigera. Pathogenicity test was conducted by conidia application of Lecanicillium sp. PTN01 on both the larvae and eggs at density of 105, 106, 107 conidia/ml. The control group was only treated with steril water. Polymerase chain reaction (PCR) was performed for molecular identification Lecanicillium sp. PTN01. The results showed that Lecanicillium sp. PTN01 able to inhibit egg hatching 13.75%, and cause mortality of first instar larval survival 98.75%. The result of virulence test to the highest larval mortality at 107 conidia/ml density was 41,25%, with the values of LT25, 50, 75 were 3.95, 7.12, 12.82 (days) and LC25, 50, 75 were 4.6 x 105, 1.7 x 106, 4.6 x 109 (conidia/ml). The DNA sequence analysis of ITS 1 and ITS 4 primers showed that Lecanicillium sp. PTN01 was similar to fungus species L. kalimantanense strain BTCC F23 with 94% homology.

Potensi fungi pelapuk putih asal lingkungan tropik untuk bioremediasi herbisida The potential white-rot fungi native of tropical environment for herbicides bioremediation Laksmita Prima SANTI; Lisdar Idwan SUDIRMAN; Didiek Hadjar GOENADI
E-Journal Menara Perkebunan Vol 75, No 1: Juni 2007
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

SummaryFungal treatment by using white-rot fungito reduce a wide variety of herbicide com-pounds is a specialized bioremediation pro-cess. A laboratory experiment was conductedto determine the ability of Phanerochaetechrysosporium, Ceriporiopsis subvermispora,and Pleurocybella porrigens and seven white-rot fungi isolated from a native of tropicalenvironment to grow on yeast malt extractglucose (YMG) agar containing highconcentration of (I) 2,4-dichlorophenoxy aceticacid, (R) glyphosate, and (G) paraquat. Thedata indicated that P. chrysosporium couldgrow on YMG media containing 5000 ppm of(I) 2,4-D, whereas BPBPI 02/04 isolate onYMG 250 ppm of (R) glyphosate or (G)paraquat. Relative values of growth inhibitionof these fungi are 81.1; 27.8; and 50.0%respectively. Biodegradation capability ofherbicides by candidate inoculants in soil-sandmedia was also determined in greenhouseexperiment by using peanut, sorghum, corn,and Borreria alata as bio-indicators. Peanutand B. alata were found to be the bestresponsive seedlings as bio-indicator on thepresence of (I) 2,4-D herbicide in soil-sandmedia.RingkasanTeknologi bioremediasi dengan fungipelapuk putih (FPP) digunakan untuk me-reduksi sejumlah senyawa herbisida. Kegiatanpenelitian yang dilakukan di laboratoriumbertujuan untuk mengetahui kemampuan tum-buh Phanerochaete chrysosporium, Ceripo-riopsis subvermispora, dan Pleurocybellaporrigens serta tujuh isolat FPP yang diperolehdari lingkungan tropik secara in vitro padamedium agar yeast malt extract glucose(YMG) yang mengandung (I) 2,4-dikloro-fenoksi asam asetat, (R) glifosat, dan (G)parakuat konsentrasi tinggi. Dari data yangdiperoleh, diketahui bahwa Ph. chrysosporiummemiliki kemampuan tumbuh dalam mediumpadat YMG yang mengandung 5000 ppm (I)2,4-D dan isolat BPBPI 02/04 pada 250 ppm(R) glifosat dan (G) parakuat dengan nilaihambatan pertumbuhan relatif terhadap kontrol(HPR) masing-masing 81,1; 27,8; dan 50,0%.Pengujian isolat terpilih terhadap kemampuanmendegradasi herbisida di dalam mediumtanah dan pasir juga dilakukan di rumah kacadengan menggunakan kacang tanah, sorgum,jagung, dan Boreria alata sebagai bioindikator.Kacang tanah dan B. alata memberikan responterbaik terhadap keberadaan herbisida (I) 2,4-Ddi dalam medium tanah dan pasir .

Kemampuan jamur pelapuk kayu isolat JPA dan Trichoderma Sp. S2-2 dalam mendegradasi tandan kosong kelapa sawit untuk menghasilkan selulosa The capability of wood rot fungus JPA isolate and Trichoderma sp. S2-2 in degradation of oil palm empty fruit bunches to produce cellulose . ALHIDAYATULLAH; Lisdar I SUDIRMAN1; Okky Setyawati DHARMAPUTRA
E-Journal Menara Perkebunan Vol 82, No 2: Desember 2014
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Abstract Oil palm empty fruit bunches (OPEFB) are the ligno-cellulosic wastes from palm oil processing. They can be used to produce raw materials for value-added products. The purpose of this study was to determine the degradation capacity of JPA wood rot fungi and Trichoderma sp. S2-2 on OPEFB. The 500 g of substrates consisted of 81% of OPEFB, 15% bran, 1.5% lime and 1.5% gypsum were used for growing. The substrates were inoculated with five treatments i.e without isolate (K); with JPA isolate (JPA); with Trichoderma sp. S2-2 (T); with the two isolates (JPA + T); and with JPA isolate and after four weeks of incubation inoculated with Trichoderma sp. S2-2 [(JPA)+T]. All treatments were incubated for eight weeks. The results showed that JPA+T was the best treatment which the two isolates must be inoculated simultaneously for degradation of OPEFB. Lignin and cellulose content on JPA+T treatment respectively were 20.83% and 33.77%. C/N ratio of OPEFB degraded with JPA+T was lower than the C/N ratio of TKKS degraded with Trichoderma harzianum and TKKS degraded with EM4 in previous study. AbstrakTandan kosong kelapa sawit (TKKS) merupakan limbah lignoselulosa dari pengolahan minyak kelapa sawit. TKKS dapat dimanfaatkan untuk memperoleh bahan baku untuk produk bernilai tambah. Tujuan penelitian ini adalah untuk mengetahui kemampuan degradasi jamur pelapuk kayu isolat JPA dan Trichoderma sp. S2-2 pada TKKS. Sebanyak 500 g substrat terdiri dari 81% TKKS, 15% dedak, 1,5% kapur, dan 1,5% gypsum digunakan untuk per-tumbuhan. Substrat diinokulasi dengan lima perlakuan yaitu tanpa isolat (K); dengan isolat JPA (JPA); dengan Trichoderma sp. S2-2 (T); dengan isolat JPA dan setelah empat minggu inkubasi, diinokulasi dengan Trichoderma sp. S2-2 [(JPA+T)]. Semua perlakuan diinkubasi selama delapan minggu. Hasil percobaan menunjukkan bahwa perlakuan JPA+T adalah perlakuan terbaik yaitu kedua isolat tesebut harus diinokulasi secara bersamaan untuk mendegradasi TKKS. Kandungan lignin dan selulosa TKKS dengan perlakuan JPA+T masing-masing adalah 20,83% dan 33,77%. Rasio C/N TKKS hasil degradasi dengan JPA+T lebih rendah daripada rasio C/N pada TKKS yang didegradasi dengan Trichoderma harzianum dan TKKS yang didegradasi dengan EM4 pada penelitian sebelumnya.

EFEKTIVITAS PENGHAMBATAN BAKTERI KITINOLITIK TERHADAP Fusarium proliferatum Siti Azzira Rahma; Nisa Rachmania Mubarik; Lisdar A Manaf
Prosiding Seminar Nasional Biotik Vol 9, No 2 (2021): PROSIDING SEMINAR NASIONAL BIOTIK IX 2021
Publisher : Prosiding Seminar Nasional Biotik

Fusarium spp. merupakan penyebab penyakit pada beberapa jenis tanaman salah satunya bawang merah sehingga dapat menurunkan produktivitas tanaman bawang merah (Allium cepa L.). Bakteri kitinolitik diketahui memiliki sifat antagonis terhadap Fusarium spp. Penelitian bertujuan mengetahui kemampuan isolat bakteri ABP5.1, ABP5.2.2, ABS4.1.2, BBP5.2.2 yang berasal dari pertanian bawang merah dalam menghambat pertumbuhan F. proliferatum. Sebanyak empat isolat bakteri terpilih hasil isolasi dari penelitian sebelumnya diuji kemampuannya dalam menghambat pertumbuhan Fusarium proliferatum. Tahapan penelitian terdiri atas karakterisasi isolat, uji aktivitas kitinolitik, uji antagonis antar isolat bakteri, dan uji antagonis isolat bakteri terhadap cendawan Fusarium proliferatum. Keempat isolat bakteri ABP5.1, ABP5.2.2, ABS4.1.2, dan BBP5.2.1 merupakan bakteri gram negatif. Uji kualitatif kitinolitik menunjukkan keempat isolat menghasilkan zona bening dengan indeks kitinolitik antara 0,04-1,0. Isolat bakteri ABS4.1.2 memiliki nilai indeks kitinolitik terbesar yaitu 1,09. Uji antagonis antar isolat bakteri menunjukkan hasil keempat isolat bakteri tidak bersifat saling antagonis. Uji antagonis bakteri terhadap cendawan Fusarium proliferatum dengan metode dual culture menunjukkan bahwa sebanyak dua dari empat isolat mampu menghambat pertumbuhan cendawan F.proliferatum. Isolat ABP5.2.2 memiliki daya hambat terbesar yaitu 41%, sedangkan isolat ABS4.1.2 memiliki daya hambat sebesar 40%.

The Initial Antibacterial Activity Tests of Pliek U Oil and Pliek U: an Acehnese Traditional Food Nurliana Nurliana; Mirnawati Sudarwanto; Lisdar I. Sudirman; A. W. Sanjaya
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): September
Publisher : Universitas Syiah Kuala

This initial research was intended to detect antibacterial activity of pliek u oil and pliek u. Pliek u oil consist of minyeuk simplah (MS) and minyeuk brok (MB), Pliek u consist of two kinds of solid waste namely wet pliek u (Ap1) and dry pliek u (Ap2). Pliek u was methanol extracted at concentration 10% (w/v). Pliek u oil and methanol extract of pliek u were evaluated for their antibacterial activity, against Bacillus subtilis and four strains of Enterophatogenic Escherichia coli (EPEC) employing agar disc diffusion method. No antibacterial activity was shown by MS. The MB exhibited a little effect 1-2 mm against bacterial tests. The results demonstrated that the higher antibacterial activity was shown by dry pliek u compare with wet pliek u with the inhibition zones from 6.67-10.33 mm and 6.00-7.33 mm respectively.

Antimicrobial Activity and LC Determination of Ethanol Crude Extract of 50 Pliek U, an Achehnese Traditional Fermented Food Nurliana Nurliana; Mirnawati Sudarwanto; Lisdar I. Sudirman; Angelina W. Sanjaya
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Antimicrobial activity of pliek u crude extracts was evaluated against five bacterial species (Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella enterica serovar Enteritidis and Pseudomonas aeruginosa,)and one fungal species (Candida albicans). Pliek u has been consumed as spices and hot sauce and poultry feed. These foods were collected from household industry at Reudep village in Aceh Besar, Province of Aceh. The ethanol extract of pliek u were obtained by standard method. The antimicrobial activity was detected using paper disc method. The concentration of ethanol crude extract of pliek u (EEP) was determined with the dilution method. The lethality initial test has been detected by using Artemia salina L. bioassay to determine the toxic concentration based on the LC value of EEP. The ethanol crude extract (EEP) was active against all microbial strains. EEP showed 50 antimicrobial activity at a minimum inhibitory concentration (MIC) and a minimum microbicidal concentration (MMC) at 2.5-10 mg/ml and 10-20 mg/ml, respectively. The lethality concentration EEP gave the LC value of 3.36 50 mg/ml. It was concluded that ethanol crude extract (EEP) shows significant antimicrobial activity and it is not toxic.

Co-Authors . Nurliana . RINDITA A. W. Sanjaya A.A. Ketut Agung Cahyawan W Alex Hartana Alhidayatullah Angelina W. Sanjaya Arifin, Sukarya Zaenal Ayni, Qurrotu Azizah Nurmalinda Azizah, Malika Budi Tjahjono Dede Setiadi Dian Syahfitri Didiek Hadjar Goenadi Evarini Anna Ratnaningsih Fahrizal Hazra Fitratul Aini Fitratul Aini, Fitratul Fitri, Devi Risvia HENNY SULISTANY Henny Sulistiany HENNY SULISTIANY, HENNY Ika Setianingsih, Ika Kartosuwondo, Utomo Laksmita Prima Santi Larasati, Afina Maria M. Misnawati Melly Fitriani Mersi Kurniati Mirnawati Sudarwanto Mirnawati Sudarwanto Munara, Yayi Nabila Tsoerayya Gustia Pudjas NISA RACHMANIA MUBARIK NURLIANA NURLIANA Nurliana Nurliana Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Prayogo, Yusmani Purnama, Meggi Rhomadona RAUF, AUNU Rika Indri Astuti RIMA ERNIA Rosita, Iros Ruly Anwar Samingan Samingan Santoso, Teguh SEMPURNA GINTING SEMPURNA GINTING Sempurna Ginting Siti Azzira Rahma Siti Nikmatin Soemartono Sosromarsono Supratman, Lilis Suryadharma Putra, Okky Teguh Santoso Trizelia . Yayi Munara Yonny Koesmaryono Yunilda Rosa YUNIMAR YUNIMAR Yusmani Prayogo Zulfiqri, Aldy Muhammad

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