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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Natur Indonesia Jurnal Agrista Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Prosiding Seminar Biologi Jurnal Sumberdaya Hayati BERITA BIOLOGI Jurnal Penelitian Sains Jurnal Penelitian Pertanian Tanaman Pangan Menara Perkebunan Prosiding Seminar Nasional Biologi, Teknologi dan Kependidikan (Biotik) Makara Journal of Science Jurnal Kedokteran Hewan Journal Of Biology Education Research (JBER)
Lisdar Idwan Sudirman
Department Of Biology, Faculty Of Mathematics And Natural Science, Bogor Agricultural University, IPB Dramaga Campus, Bogor, West Java
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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The Initial Antibacterial Activity Tests of Pliek U Oil and Pliek U: an Acehnese Traditional Food Nurliana Nurliana; Mirnawati Sudarwanto; Lisdar I. Sudirman; A. W. Sanjaya
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i2.9781

Abstract

This initial research was intended to detect antibacterial activity of pliek u oil and pliek u. Pliek u oil consist of minyeuk simplah (MS) and minyeuk brok (MB), Pliek u consist of two kinds of solid waste namely wet pliek u (Ap1) and dry pliek u (Ap2). Pliek u was methanol extracted at concentration 10% (w/v). Pliek u oil and methanol extract of pliek u were evaluated for their antibacterial activity, against Bacillus subtilis and four strains of Enterophatogenic Escherichia coli (EPEC) employing agar disc diffusion method. No antibacterial activity was shown by MS. The MB exhibited a little effect 1-2 mm against bacterial tests. The results demonstrated that the higher antibacterial activity was shown by dry pliek u compare with wet pliek u with the inhibition zones from 6.67-10.33 mm and 6.00-7.33 mm respectively.
Antimicrobial Activity and LC Determination of Ethanol Crude Extract of 50 Pliek U, an Achehnese Traditional Fermented Food Nurliana Nurliana; Mirnawati Sudarwanto; Lisdar I. Sudirman; Angelina W. Sanjaya
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9795

Abstract

Antimicrobial activity of pliek u crude extracts was evaluated against five bacterial species (Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella enterica serovar Enteritidis and Pseudomonas aeruginosa,)and one fungal species (Candida albicans). Pliek u has been consumed as spices and hot sauce and poultry feed. These foods were collected from household industry at Reudep village in Aceh Besar, Province of Aceh. The ethanol extract of pliek u were obtained by standard method. The antimicrobial activity was detected using paper disc method. The concentration of ethanol crude extract of pliek u (EEP) was determined with the dilution method. The lethality initial test has been detected by using Artemia salina L. bioassay to determine the toxic concentration based on the LC value of EEP. The ethanol crude extract (EEP) was active against all microbial strains. EEP showed 50 antimicrobial activity at a minimum inhibitory concentration (MIC) and a minimum microbicidal concentration (MMC) at 2.5-10 mg/ml and 10-20 mg/ml, respectively. The lethality concentration EEP gave the LC value of 3.36 50 mg/ml. It was concluded that ethanol crude extract (EEP) shows significant antimicrobial activity and it is not toxic.
The Potential of Endophytic Bacteria as the Biocontrol Agents of Fusarium proliferatum Ernia, Rima; Mubarik, Nisa Rachmania; Sudirman, Lisdar Idwan
Makara Journal of Science Vol. 27, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Fusarium proliferatum decreases the productivity of shallot (Allium cepa L.). Endophytic bacteria are potential biocontrol agents in controlling the growth of F. proliferatum. This study aimed to select and characterize endophytic bacteria producing bioactive compounds that inhibit the growth of F. proliferatum. The endophytic bacteria used were isolated from the roots, stems, leaves, and soil of shallot plants from healthy and diseased shallot farming fields. The endophytic bacteria were selected using the dual culture method and extracted using ethyl acetate solvent. The results of the 16S rRNA identification suggested that isolate ABP5.2 is similar to Pseudomonas aeruginosa, while isolates BBP5.2 and DBP4.1 are similar to Myroides profundi. Isolate BBP5.2 was found to have the highest ability (68.15%) to inhibit the growth of F. proliferatum by using its antifungal compounds through a direct mechanism. In addition, the results of the Gas Chromatography-Mass Spectrophotometry analysis suggested that the compounds produced by the crude extracts of BBP5.2 isolate are hexadecanoic acid, 9-octadecenoic acid, linoleic acid, and piperine.
Kandungan Gizi Jamur Tiram pada Substrat Kayu Sengon dan Klaras Pisang: Nutrition Contents of Oyster Mushroom on Sengon Wood and Banana Leaves Substrates Azizah, Malika; Sudirman, Lisdar Idwan; Arifin, Sukarya Zaenal; Setianingsih, Ika; Larasati, Afina; Zulfiqri, Aldy Muhammad
Jurnal Sumberdaya Hayati Vol. 8 No. 2 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.2.57-64

Abstract

The study was conducted to observe the growth, fruiting body production and nutritional contents of white oyster mushroom (Pleurotussp., wild HS isolate) grown on 300 g of mixed substrate between sengon sawdust (Albizia chinensis) (SGKS) and dried banana leaves or klaras (Musa sp.) and compared with SGKS, the commonly used single substrate. Both substrates were added with 15% bran, 1.5% lime and 1.5% gypsum. The production of mushroom fruiting bodies on mixed substrates was higher than that of single substrate with biological efficiency of 27.24% and 22.00%, respectively. Likewise, the protein content of the mixed substrate was much higher than that of the single substrate, 19.6% and 10.9%, respectively, while the crude fiber and carbohydrate contents were slightly higher in the single substrate, respectively 8.76% and 54 ,37%. Further research needs to be carried out on a mixed substrate of at least 500 g to get a more complete pattern of the production and biological efficiency.
Inventarisasi Awal Liken Langka, Sensitif dan Toleran sebagai Bioindikator Polutan di Kabupaten Belitung Barat : Preliminary Inventory of Rare, Sensitive and Tolerant Lichens as Bioindicators of Pollutants in West Belitung Regency I Sudirman, Lisdar; Fitri, Devi Risvia; Purnama, Meggi Rhomadona
Jurnal Sumberdaya Hayati Vol. 10 No. 2 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.2.102-107

Abstract

The existence and population conditions of the rare, sensitive or tolerant lichens of Lobaria, Coccocarpia, Coenogonium, Dictyonema, Pseudocyphellaria, Sticta, Usnea, Leptogium, and Parmotrema tinctorum are not yet known in West Belitung Regency, so this research needs to be carried out. The method used in this research was exploratory descriptive with survey techniques and purposive sampling. The number of target lichen thallus was calculated from the entire area of the tree trunk with a height of 0-1.5 m. The lichens found in the Gunung Tajam area were Coccocarpia sp. with the highest number of thallus and the highest frequency of thallus (0.8%), followed by Coenogonium sp., Leptogium sp., and Usnea sp. with the same frequency of thallus (0.4%) while in the Batu Mentas area lichens were not found as the research target, but was dominated by fruticose lichen. Parmotrema tinctorum, the tolerant lichen, was not found at either location. The presence of sensitive lichens and the absence of tolerant lichens does not indicate that the locations have good air quality. It is recommended that the lichen population, especially P. tinctorum, be studied further so that air quality can be easily confirmed through the diversity and population of the lichens.
Produksi Jamur Merang (Volvariella volvacea (Bull. ex. Fr) Sing). pada Dua Jenis Limbah Kapas: Production of Straw Mushrooms (Volvariella volvacea (Bull. ex. Fr) Sing). on Two Types of Cotton Wastes Sudirman, Lisdar Idwan; Rosita, Iros; Dharmaputra, Okky Setyawati
Jurnal Sumberdaya Hayati Vol. 10 No. 4 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.4.229-237

Abstract

One of the substrates for the growth of straw mushrooms is cotton waste. Based on the cellulose content, trash cotton and dust cotton were selected as substrates. This research was conducted twice at the dry season. The 3,000 g production substrates were a mixture of trash cotton and straw in a ratio of 2:1 (T2J1) and 1:1 (T1J1), dust cotton and straw in a ratio of 2:1 (D2J1) and 1:1 (D1J1). Previously, cotton and straw with the addition of 8% rice bran and 3% lime were composted separately, then mixed, pasteurized and inoculated with 2.5% of spawn. The D2J1 substrate produced higher number and bigger of fruit bodies, with the biological efficiency (BE) of 21.22 and 23.03% for the first and the second experiments, while the BE of T1J1 were only 16.76% and 5.84% and those of D1J1 were 15.63% and 12.45%, those of T2J1 were 16.76% and 0.22% for the first and second experiment respectively. The lowest BE value in these experiments due to incomplete vegetative phase, along with the contamination of Trichoderma sp. In conclusion, the production of straw mushroom fruiting bodies depends on the type of cotton and its ratio to straw.
Seleksi dan Pengujian Bakteri Biokontrol terhadap Cendawan Patogen Fusarium sp. yang Diisolasi dari Buah Tomat (Solanum Lycopersicum L.): Selection and Testing of Biocontrol Bacteria against Pathogenic Fungi Fusarium sp. Isolated from Tomato Fruits (Solanum lycopersicum L.) Ayni, Qurrotu; Mubarik, Nisa Rachmania; Sudirman, Lisdar I
Jurnal Sumberdaya Hayati Vol. 11 No. 1 (2025): 2025
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.11.1.23-30

Abstract

Fusarium oxysporum is one of the primary pathogens in tomato plants that causes plant wilting. Pathogenic fungi have been controlled by spraying synthetic fungicides. The negative impact of excessive use of synthetic chemicals can be reduced by using biocontrol agents that produce antifungal compounds. Natural pest control efforts use endophytic bacteria and rhizosphere as biocontrol agents for fungal pathogens on tomato plants (Solanum lycopersicum L). This study aims to select and test biocontrol bacteria against pathogenic fungi Fusarium sp. isolated from tomato fruits. Fusarium successfully isolated from tomato fruits which was characterized based on observations of colony and cell morphology. A total of 15 rhizosphere bacterial isolates were tested against Fusarium sp., and 11 bacterial isolates were able to inhibit the growth of the fungus. Isolates TTSG 2.2, TTSG 2.7, TTSG 3.2, TTSG 3.5, TTSG 3.6, and TCS 3.1 had fungal inhibition of 45%. The results of the hemolysin test showed that one isolate, TTSG 3.6 isolate, was negative, which did not form a clear zone around the bacterial streak. Isolate TTSG 3.6 still showed a few brown spots and rotted tomato fruit, so this bacteria needs to be tested for hypersensitivity on tobacco leaves to show its pathogenicity in plants.
Kemampuan jamur pelapuk kayu isolat JPA dan Trichoderma Sp. S2-2 dalam mendegradasi tandan kosong kelapa sawit untuk menghasilkan selulosa The capability of wood rot fungus JPA isolate and Trichoderma sp. S2-2 in degradation of oil palm empty fruit bunches to produce cellulose . ALHIDAYATULLAH; Lisdar I SUDIRMAN1; Okky Setyawati DHARMAPUTRA
Menara Perkebunan Vol. 82 No. 2: 82 (2), 2014
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v82i2.17

Abstract

Abstract  Oil palm empty fruit bunches (OPEFB) are the ligno-cellulosic wastes from palm oil processing. They can be used to produce raw materials for value-added products. The purpose of this study was to determine the degradation capacity of JPA wood rot fungi and Trichoderma sp. S2-2 on OPEFB. The 500 g of substrates consisted of 81% of OPEFB, 15% bran, 1.5% lime and 1.5% gypsum were used for growing. The substrates were inoculated with five treatments i.e without isolate (K); with JPA isolate (JPA); with Trichoderma sp. S2-2 (T); with the two isolates (JPA + T); and with JPA isolate and after four weeks of incubation inoculated with Trichoderma sp. S2-2 [(JPA)+T]. All treatments were incubated for eight weeks. The results showed that JPA+T was the best treatment which the two isolates must be inoculated simultaneously for degradation of OPEFB. Lignin and cellulose content on JPA+T treatment respectively were 20.83% and 33.77%. C/N ratio of OPEFB degraded with JPA+T was lower than the C/N ratio of TKKS degraded with Trichoderma harzianum and TKKS degraded with EM4 in previous study. AbstrakTandan kosong kelapa sawit (TKKS) merupakan limbah lignoselulosa dari pengolahan minyak kelapa sawit. TKKS dapat dimanfaatkan untuk memperoleh bahan baku untuk produk bernilai tambah. Tujuan penelitian ini adalah untuk mengetahui kemampuan degradasi jamur pelapuk kayu isolat JPA dan Trichoderma sp. S2-2 pada TKKS. Sebanyak 500 g substrat terdiri dari 81% TKKS, 15% dedak, 1,5% kapur, dan 1,5% gypsum digunakan untuk per-tumbuhan. Substrat diinokulasi dengan lima perlakuan yaitu tanpa isolat (K); dengan isolat JPA (JPA); dengan Trichoderma sp. S2-2 (T); dengan isolat JPA dan setelah empat minggu inkubasi, diinokulasi dengan Trichoderma sp. S2-2 [(JPA+T)]. Semua perlakuan diinkubasi selama delapan minggu. Hasil percobaan menunjukkan bahwa perlakuan JPA+T adalah perlakuan terbaik yaitu kedua isolat tesebut harus diinokulasi secara bersamaan untuk mendegradasi TKKS. Kandungan lignin dan selulosa TKKS dengan  perlakuan  JPA+T   masing-masing  adalah  20,83% dan 33,77%. Rasio C/N TKKS hasil degradasi dengan JPA+T lebih  rendah  daripada  rasio C/N pada TKKS yang didegradasi dengan Trichoderma harzianum dan TKKS yang didegradasi dengan EM4 pada penelitian sebelumnya.
Potensi fungi pelapuk putih asal lingkungan tropik untuk bioremediasi herbisida The potential white-rot fungi native of tropical environment for herbicides bioremediation Laksmita Prima SANTI; Lisdar Idwan SUDIRMAN; Didiek Hadjar GOENADI
Menara Perkebunan Vol. 75 No. 1: 75 (1), 2007
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v75i1.153

Abstract

SummaryFungal treatment by using white-rot fungito reduce a wide variety of herbicide com-pounds is a specialized bioremediation pro-cess. A laboratory experiment was conductedto determine the ability of Phanerochaetechrysosporium, Ceriporiopsis subvermispora,and Pleurocybella porrigens and seven white-rot fungi isolated from a native of tropicalenvironment to grow on yeast malt extractglucose (YMG) agar containing highconcentration of (I) 2,4-dichlorophenoxy aceticacid, (R) glyphosate, and (G) paraquat. Thedata indicated that P. chrysosporium couldgrow on YMG media containing 5000 ppm of(I) 2,4-D, whereas BPBPI 02/04 isolate onYMG 250 ppm of (R) glyphosate or (G)paraquat. Relative values of growth inhibitionof these fungi are 81.1; 27.8; and 50.0%respectively. Biodegradation capability ofherbicides by candidate inoculants in soil-sandmedia was also determined in greenhouseexperiment by using peanut, sorghum, corn,and Borreria alata as bio-indicators. Peanutand B. alata were found to be the bestresponsive seedlings as bio-indicator on thepresence of (I) 2,4-D herbicide in soil-sandmedia.RingkasanTeknologi bioremediasi dengan fungipelapuk putih (FPP) digunakan untuk me-reduksi sejumlah senyawa herbisida. Kegiatanpenelitian yang dilakukan di laboratoriumbertujuan untuk mengetahui kemampuan tum-buh Phanerochaete chrysosporium, Ceripo-riopsis subvermispora, dan Pleurocybellaporrigens serta tujuh isolat FPP yang diperolehdari lingkungan tropik secara in vitro padamedium agar yeast malt extract glucose(YMG) yang mengandung (I) 2,4-dikloro-fenoksi asam asetat, (R) glifosat, dan (G)parakuat konsentrasi tinggi. Dari data yangdiperoleh, diketahui bahwa Ph. chrysosporiummemiliki kemampuan tumbuh dalam mediumpadat YMG yang mengandung 5000 ppm (I)2,4-D dan isolat BPBPI 02/04 pada 250 ppm(R) glifosat dan (G) parakuat dengan nilaihambatan pertumbuhan relatif terhadap kontrol(HPR) masing-masing 81,1; 27,8; dan 50,0%.Pengujian isolat terpilih terhadap kemampuanmendegradasi herbisida di dalam mediumtanah dan pasir juga dilakukan di rumah kacadengan menggunakan kacang tanah, sorgum,jagung, dan Boreria alata sebagai bioindikator.Kacang tanah dan B. alata memberikan responterbaik terhadap keberadaan herbisida (I) 2,4-Ddi dalam medium tanah dan pasir .
Lichen Pseudocyphellaria Mapping in Cibodas Botanical Garden Conservation Park Supratman, Lilis; Sudirman, Lisdar; Suryadharma Putra, Okky
Journal Of Biology Education Research (JBER) Vol. 6 No. 1 (2025): JBER (Journal Of Biology Education Research), Vol. 6 No. 1 (2025)
Publisher : Program Studi Pendidikan Biologi FKIP Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55215/jber.v6i1.31

Abstract

Lichens are symbiotic organisms between fungi and algae. In Indonesia, lichen inventory research is very limited. Several Lichen families have entered critical levels such as Lobariaceae. The purpose of this study was to: record and collect the Lobariaceae family as a rare lichen, analyze population data of the Lobariaceae family, growth percentage and development of Lobariaceae family transplants. This research method used exploration with calculations based on the surface area of ​​tree trunks, the number and coverage of thallus, and thallus coverage per square meter for each tree. The results of this study found 340 Pseudocyphellaria thallus consisting of Pseudocyphellaria sp. P. aurata, P. crocata and P. argyracea. During one year of Pseudocyphellaria transplantation, there was no growth in terms of increasing thallus cover. The development that occurred was that the transplant was able to attach well to the substrate. It is estimated that Pseudocyphellaria thallus takes longer to adapt well to the substrate to grow and develop. The conclusion of this study is that the transplanted lichen showed satisfactory growth, which indicates that this method can be used for the conservation of threatened lichen species.    
Co-Authors . Nurliana . RINDITA A. W. Sanjaya A.A. Ketut Agung Cahyawan W Alex Hartana Alhidayatullah Angelina W. Sanjaya Arifin, Sukarya Zaenal Ayni, Qurrotu Azizah Nurmalinda Azizah, Malika Budi Tjahjono Dede Setiadi Dian Syahfitri Didiek Hadjar Goenadi Evarini Anna Ratnaningsih Fahrizal Hazra Fitratul Aini Fitratul Aini, Fitratul Fitri, Devi Risvia HENNY SULISTANY Henny Sulistiany HENNY SULISTIANY, HENNY Ika Setianingsih, Ika Kartosuwondo, Utomo Laksmita Prima Santi Larasati, Afina Maria M. Misnawati Melly Fitriani Mersi Kurniati Mirnawati Sudarwanto Mirnawati Sudarwanto Munara, Yayi Nabila Tsoerayya Gustia Pudjas NISA RACHMANIA MUBARIK Nurliana Nurliana NURLIANA NURLIANA Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Okky Setyawati Dharmaputra Prayogo, Yusmani Purnama, Meggi Rhomadona RAUF, AUNU Rika Indri Astuti RIMA ERNIA Rosita, Iros Ruly Anwar Samingan Samingan Santoso, Teguh SEMPURNA GINTING SEMPURNA GINTING Sempurna Ginting Siti Azzira Rahma Siti Nikmatin Soemartono Sosromarsono Supratman, Lilis Suryadharma Putra, Okky Teguh Santoso Trizelia . Yayi Munara Yonny Koesmaryono Yunilda Rosa YUNIMAR YUNIMAR Yusmani Prayogo Zulfiqri, Aldy Muhammad