Article Per Year (5 Year)
p-Index From 2021 - 2026
1.713
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences VALENSI Sarwahita : Jurnal Pengabdian Kepada Masyarakat Jurnal Riset Sains dan Kimia Terapan JSMARTech : Journal of Smart Bioprospecting and Technology Makara Journal of Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Kesehatan Masyarakat Jurnal Bioteknologi & Biosains Indonesia
Putri, Gladys Indira
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

Published : 11 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 11 Documents
Search

 1   2 
Development of Shigella flexneri Detection Method by Real-Time Polymerase Chain Reaction Targeting the sfmD Gene Nurjayadi, Muktiningsih; Azizah, Puan Aqila; Setiawan, Agus; Sihombing, Ananda Indah Putri; Fitriyanti, Anisa; Grace, Grace; Putri, Gusti Angieta; Angelina, Helzi; Musie, Royna Rahma; Declan, Jefferson Lynford; Putri, Gladys Indira; Juliansyah, Dandy Akbar; Fatimah, Siti; Fahriza, Tiara; Kartika, Irma Ratna; Kurniadewi, Fera; Novitasari, Novitasari; Abomoelak, Bassam; El Enshasy, Hesham Ali; Chen, Shyi-Tien
Makara Journal of Science Vol. 29, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Shigella flexneri can cause shigellosis, which results in high fever, vomiting, diarrhea, or death. This study developed a detection method based on real-time polymerase chain reaction that targets the sfmD gene of S. flexneri given that the virulence factor encoded by this gene is thought to facilitate adhesion to a host surface. The proposed method involves primer design, DNA isolation, the optimization of primer annealing temperature, and confirmation, specificity, and sensitivity tests. A sfmD primer with an amplicon length of 155 bp was annealed to the target DNA at an optimized temperature of 60 ℃ (range 54 °C–62 °C). This primer pair amplified the target sequences at a cycle threshold (Ct) of 15.11 ± 0.38 and a melting temperature of 82.41 ± 0.01 ℃. The primer specificity test showed that the primer distinguished S. flexneri from nontarget bacteria. The findings also revealed that the primer detected S. flexneri down to a limit of 16 pg/μL at a Ct of 26.68, equivalent to 2.79×102 CFU. Overall, the sfmD primer can effectively amplify S. flexneri DNA. Future research can be directed toward the detection of S. flexneri in artificially contaminated food samples to validate the real-food applicability and strengthen its potential use in food safety monitoring and clinical diagnostics.
Co-Authors Aboemolak, Bassam Abomoelak, Bassam Abumoelak, Bassam Akbar, Dandy Angelina, Helzi Anggraeni, Rosita Gio Azizah, Puan Aqila Azzahra, Maharanianska Berkahingrum, Ayu Chen, Shyi-Tien Dalia Sukmawati El Enshasy, Hesham El Enshasy, Hesham Ali El-Enshasy, Hesham Ali Elenshasy, Hesham Ali Enshasy, Hesham Ali El Fahriza, Tiara Fitriyanti, Anisa Grace, Grace Hairishah, Nazrisya Jefferson Lynford Declan Juliansyah, Dandy Akbar Kartika, Irma Ratna Krisdawati, Ismaya Kurniadewi, Fera Maulana, Irvan Muktiningsih Nurjayadi Musie, Royna Rahma NOVITASARI Novitasari Novitasari Putri, Adinda Myra Amalia Putri, Gusti Angieta Rahmawati, Atikah Nur Saamia, Vira Saamia4, Vira Saputro, Dwi Anna Oktaviani Shangkara, Muhammad Arkent Sihombing, Ananda Indah Putri Siti Fatimah SRI RAHAYU Wiranatha, I Made