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All Journal International Journal of Public Health Science (IJPHS) At-Taqaddum THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Biologi Tropis Conference on Innovation and Application of Science and Technology (CIASTECH) Klinikal Sains (Jurnal Analis Kesehatan) JURNAL KESEHATAN PERINTIS Jurnal Pelayanan dan Pengabdian Masyarakat (Pamas) JURNAL ANALIS LABORATORIUM MEDIK Jurnal Peduli Masyarakat Indonesian Journal of Global Health research Medicra (Journal of Medical Laboratory Science/Technology) International Journal of Health Science and Technology
Muhammad Taufiq Qurrohman, Muhammad Taufiq
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Profile of MYH9 SNP RS3752462 Gene Polymorphism in Chronic Kidney Disease (CKD) Patients with Hypertension Heryanti, Nabiilah Nuur Ainii; Qurrohman, Muhammad Taufiq
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11711

Abstract

Chronic kidney disease (CKD) and hypertension are closely related conditions and can be influenced by genetic factors, including polymorphisms in the Myosin Heavy Chain 9 (MYH9) gene. The SNP rs3752462 in the MYH9 gene is known to be associated with impaired kidney function. This study aims to describe the genotype pattern of the MYH9 rs3752462 SNP polymorphism in patients with chronic kidney disease and hypertension at UNS Hospital. This is a descriptive study with a molecular analysis approach. A total of 10 blood samples were collected. Polymorphism detection was performed using the Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR-RFLP) method with the RsaI restriction enzyme. The results showed that all samples were successfully amplified, producing a PCR product of approximately 421 bp. The DNA bands observed in all samples did not form a consistent combination and did not match the expected genotype reference standard. In addition, the appearance of additional bands outside the target fragment size, therefore the results of this study do not adequately represent the MYH9 SNP rs3752462 gene polymorphism in the studied samples. Conclusion: Further examination is needed using other modern methods such as DNA sequencing and larger sample sizes to obtain appropriate results.
Overview of MYH9 Gene Polymorphism SNP RS3752462 in Chronic Kidney Disease (CKD) Patients with Diabetic Nephropathy Maulana, Ahmad Akmal; Qurrohman, Muhammad Taufiq
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11712

Abstract

Diabetic nephropathy is a major cause of chronic kidney disease and is influenced by both environmental and genetic factors. One of the genes implicated in the pathogenesis of diabetic nephropathy is MYH9, particularly the Single Nucleotide Polymorphism (SNP) rs3752462. This study aimed to describe the polymorphism of the MYH9 gene SNP rs3752462 in patients with chronic kidney disease associated with diabetic nephropathy. A descriptive study design was applied, involving five patients who met the inclusion criteria. Blood samples were analyzed using the Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR-RFLP) method with the RsaI restriction enzyme. The Results showed that the DNA band patterns observed in all samples did not form consistent combinations and did not correspond to the expected genotype classification standards. In addition, extra bands outside the target fragment sizes and variations in band intensity among samples were observed. Based on these findings, the resulting band patterns could not be reliably used to accurately determine genotypes, and therefore did not adequately represent the polymorphism of the MYH9 gene SNP rs3752462 in the studied samples. Accordingly, further optimization of the PCR-RFLP method is required, along with validation using more accurate approaches, such as DNA sequencing, and a larger sample size to obtain more reliable results.
Co-Authors Annisa, Mariana Suryanti Nur Cahyanti, Eva Dewangga, Vector Stephen Dewangga, Vector Stephen Dhiya, Salsabila Shafa Exzora, Helmalia Bilqis Fadhilla, Nadyah Saffana Faizha, Naela Dinda Fitri, Syifa Aurelya Hastuti, Novita Dwi Heryanti, Nabiilah Nuur Ainii Khasanah, Novita Uswatun Maharani, Raras Maulana, Ahmad Akmal Mega Fatimah Rosana Muhammad Thoriq Aulia Khamim Murwani, Antik Ningrum, Anik Puspita Noviana Dewi Priyandari, Damar Ajeng Pujianti, Yunita Putri Putra, Muhammad Andriyansyah Sanky Putri, Adila Purnama Putri, Alya Ernita Rahmawati, Desinta Ramadhan, Intan Nur Rasid, Bryan Aditya Nur Renatasya Silviana Herningtyas Rizki, Andita Ainal Sanita, Hanifah Velda Sari, Monica Ayu Retno Sawitri, Debora Sayekti, Fitria Diniah Janah Shaleha, Annisa Azizah Sitohang, Dahlan Srikandini, Chris Tambunan, Yohana Nathalia Tanaya, Angabaya Dera Sumba Wijayanto, Mustofa Tri Yasinta, Tia Yoga Pratama Putra