Irene E. Rieuwpassa, Irene E.
Department Of Oral Biology, Faculty Of Dentistry, Hasanuddin University, Makassar, Indonesia

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Deteksi Mutasi Gen Gyrase A Porphyromonas Gingivalis Resisten terhadap Ciprofloxacin berdasarkan teknik Polymerase Chain Reaction E.Rieuwpassa, Irene; Hatta, Mochammad
Jurnal Kedokteran YARSI Vol 17, No 1 (2009): JANUARI - APRIL 2009
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (753.675 KB) | DOI: 10.33476/jky.v17i1.192

Abstract

One of resistance mechanisms to ciprofloxacin shown by bacterium Porphyromonas gingivalis isolated from periodontitis patients is mutations of genes through changes in DNA topoisomerase. Ciprofloxacin is an effective antimicrobial for Gram-negative bacteria effectively used for clinical infections treatment. The purpose of this research was to determine the gene mutation of P. gingivalis on periodontitis patients in relation to the resistance to ciprofloxacin by using disc diffusion and, followed by RFLP-PCR on P.gingivalis samples. Based on sensitivity tests by diffusion method, it was shown that the sensitive samples (n =13) had the highest inhibition zone diameter (mean= 25 mm), while the intermediate samples (n=1) was 19 mm. Examination using RFLP-PCR for 14 samples did not reveal any mutation of gyrase A gene.
Mouth preparation pasien hemangioma bibir pada anak sebelum tindakan radioterapi Irene Edith Rieuwpassa; Harun Achmad
Journal of Dentomaxillofacial Science Vol. 9 No. 2 (2010): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v9i2.237

Abstract

Hemangioma is a vascular benign tumor frequently found in childhood and about30% manifests itself in head and neck areas. This lesion is frequently found in lip,tongue and buccal mucosal areas. Its etiology is conjectured to be related to theproliferation of abnormalities of endothelium cells. According to its type, hemangiomais divided into capillary, cavernous, and mixed hemangioma. Surgery is indicated incases that cause complications. A case of hemangioma was reported in the lower lipof an eight-year-old girl. The patient accompanied by her parents visited the SpecialDental Care Clinic, Department of Oral Surgery, Hasan Sadikin Hospital, Bandung,with the complaint of having reddish bruises on the lower lip, which impeded thespeaking and masticating function. Before radiotherapy was performed at theRadiology Polyclinic, mouth preparation was provided for all his teeth first, for thepurpose of improving oral hygiene and preventing focal infection present in theintraoral condition.
Mahasiswa tingkat kepaniteraan Universitas Hasanuddin Makassar, Indonesia Irene Edith Rieuwpassa; Rahmat Rahmat; Karlina Karlina
Journal of Dentomaxillofacial Science Vol. 10 No. 2 (2011): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v10i2.256

Abstract

The aim of this study was to determine the effectiveness of Aloe vera extract in inhibiting the growth ofStaphylococcus aureus and to measure the minimum inhibitory concentration (MIC) on the growth of the bacteria.The method using the manufacture of aloe extract, extract dilution, purified Staphylococcus aureus, MIC test andview the zone of inhibition. The results show that the (MIC) of Aloe extract was at concentration of 0.25. After astatistical test with ANOVA test on survey data (α<0.05), then the result obtained 0.000. This shows significantdifferences between various concentrations of Aloe vera extract in inhibiting the growth of Staphylococcus aureusbacteria. The higher the concentrationof Aloe vera extract, the more expand diameter of inhibition zone. Thusextracts of Aloe vera may be used to inhibit the growth of Staphylococcus aureus.
Identifikasi Pseudomonas aeruginosa dan tes sensitivitas siprofloksasin pada abses periodontal Identification of Pseudomonas aeruginosa and sensitivity test of ciprofloxacin on periodontal abcess Irene E. Rieuwpassa; Muliaty Yunus; I Wayan Suka Arsana
Journal of Dentomaxillofacial Science Vol. 10 No. 3 (2011): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v10i3.274

Abstract

Periodontitis is a common type of periodontal disease caused by expansion of the early stages of gingivalinflammation. Expansion of inflammation to the tissue structures supporting the teeth can be modified by thepathogenic ability of plaque or host resistance factors. A total of 200 different bacteria have been identified on theplaque. Resistance to antimicrobials can be natural because the microbes develop mechanisms to defendthemselves. Ciprofloxacin is a synthetic drug of the second generation quinolones derivatives. Mechanism of itsaction is to inhibit the activity of bacterial DNA gyrase, which is bactericidal with a broad spectrum against Grampositiveor negative. This observational study identified P. aeruginosa and sensitivity test was performed tociprofloxacin in periodontal abscesses. Study conducted in 23 patients with periodontal abscess. Of those,Pseudomonas was acquired for 8 samples and 4 of them was resistant to ciprofloxacin.
Perbedaan ukuran dan bentuk lengkung gigi antara laki-laki dan perempuan suku Bugis, Makassar, dan Toraja Difference of size and shape of dental arch between male and female of Buginese, Makassarese, and Toraja Irene Edith Rieuwpassa; Syamsiar Toppo; Sri Dian Haerawati
Journal of Dentomaxillofacial Science Vol. 11 No. 3 (2012): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v11i3.330

Abstract

Factors which influence the dental arch changes including genetic and environment. This study aims to determine thedifferences in size and shape of dental arch between men and women of Buginese, Makassarese and Torajanese. Thisstudy is an observational study, with a cross-sectional study design. The sampling method was purposive sampling, andnumber of samples were 90 samples obtained from 90 people with 30 people for each tribe, consist of 15 men and 15women, aged 18-25 years, with full dentition. Data were obtained through tcasting models of the maxillary andmandibular samples then were measured by using a ruler and caliper, then calculated using formula to determine,register, and analyze the size and shape of dental arch. Using multiple statistics, including t-test, ANOVA, chi-squaretest, and marginal of homogenity test there are significant differences regarding the size and shape of dental archbetween men and women of the Buginese, Makassarese, and Torajanese. There is only significant difference ofmaxillary dental arch for Buginese, Makassarese, and Torajanese
Ukuran mesiodistal dan servikoinsisal gigi insisivus sentralis suku Bugis, Makassar, dan Toraja tidak menunjukkan perbedaan yang bermakna Size of mesiodistal and cervicoincisal maxillary central incisors between Buginese, Makassarese, and Torajanese showed no significant difference Irene Edith Rieuwpassa; Nurlindah Hamrun; Fitrian Riksavianti
Journal of Dentomaxillofacial Science Vol. 12 No. 1 (2013): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v12i1.339

Abstract

Every tribe and race has size of tooth mesiodistal and cervicoincisal different from each other. The most obviousdifference is the size of the teeth between female and male. This study aimed to determine differences in size ofmesiodistal and cervicoincisal maxillary central incisors between male and female of Buginese, Makassarese andTorajanese. The sample consisted of 30 Buginese, 30 Makassarese, and 30 Torajanese aged frem 18-25 years-old.After impression of the upper and lower jaw was taken, and size of mesiodistal and cervicoincisal tooth weremeasured. After the data were analyzed by t-test and ANOVA test, it appears that males have a larger size thanfemales. Makassarese has the longest mesiodistal size (8.41 mm), Buginese has the longest cervicoincisal size (9.83mm), whereas Torajanese has the smallest size of mesiodistal and cervicoincisal among the other tribes. It was concluded that the average sizes of mesiodistal and cervicoincisal of maxillary central incisors showed no significantdifference. While there are significant differences in the average size of the mesiodistal and cervicoincisal maxillarycentral incisors between the males and females, and based on tribe lines.
Ekstrak buah kaktus pir berduri menghambat pertumbuhan Staphylococcus aureus, Streptococcus mutans, dan Candida albicans Extract of cactus prickly pear inhibits the growing of Staphylococcus aureus, Streptococcus mutans, and Candida albicans Irene Edith Rieuwpassa; Nurlindah Hamrun; St. Rahma Lukman; Reski Y. S; Soelistia Ramadhani
Journal of Dentomaxillofacial Science Vol. 12 No. 3 (2013): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v12i3.367

Abstract

The study aimed to determine the effect of prickly pear cactus extract in inhibiting the growth of Staphylococcusaureus, Streptococcus mutans and Candida albicans. The laboratory experimental study using diffusion method. Theconcentration of the prickly pear cactus fruit extract used was 25%, 50%, 75%, 100%, whereas bacterial samplesderived from the laboratory of Microbiology, Faculty of Medicine, University of Hasanuddin. The observation ofseveral concentrations showed a decrease in the number of colonies contained S.aureus, S.mutans, C.albicanssignificant with increasing concentrations of the prickly pear cactus fruit extract. From these observations, thelargest inhibitory concentration present in a concentration of 100%. The conclusion is the fruit of the prickly pearcactus extract can inhibit the growth of S.aureus, S.mutans, and C.albicans. The higher the concentration of theextract, the more reduced the growth of S.aureus colonies, S.mutans, and C.albicans and vice versa.
Gambaran sidik bibir mahasiswa pada Fakultas Kedokteran Gigi Universitas Hasanuddin (Lip print imaging of students at Faculty of Dentistry Hasanuddin University) Syamsiar Toppo; Irene Edith Rieuwpassa; J. I. Lisal; Ummi Salmiah Sari
Journal of Dentomaxillofacial Science Vol. 13 No. 1 (2014): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v13i1.380

Abstract

Cheiloscopy is the study of lip print used to identify a person. Like finger print, lip print patterns recently are proven tohave differences in each individual. Groove on the lip is fixed and cannot be changed. Following Suzuki method, lipprint pattern is divided into 6 types, namely complete vertical groove, incomplete vertical groove, branched groove,groove intersected, groove reticular, and undifferianted. The purpose of this study was to determine the lip printpattern of Indonesian people, especially the students of the Faculty of Dentistry, University of Hasanuddin. Thisresearch was conducted at the Faculty of Dentistry, Hasanuddin University. Using random sampling technique, thesample of the study consisted of 50 subjects that include men and women in the age group 20-24 years. The lips thathave been smeared with lipstick are open on its normal position, smile and the kiss is record. Masking tape is attachedat various positions on the lips and then the tape is attached to the paper to be observed. Data are analysed with amagnifying glass and classified according to the "Classification Tsuchihashi and Suzukis. The study found all lip printsshow different patterns. The pattern of lip prints is not only made up of only one type of groove, but appears as amixture of various types. The pattern of lip prints is generally found in type II and type V is the least found. The studyconcludes that no difference in the pattern of individual lip prints on a wide range of positions in the normal, open,smile and kiss positions.
Comparison of enamel hardness after dental bleaching agent application strawberry gel and carbamide peroxide 10% Asmawati Asmawati; Irene E. Rieuwpassa
Journal of Dentomaxillofacial Science Vol. 3 No. 1 (2018): (Available online: 1 April 2018)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v3i1.626

Abstract

Objective: Carbamide peroxide 10% is commonly used in home bleaching procedure. Effectiveness of carbamide peroxide bleaching process as a home bleaching has no successor yet, but its use is still being debated due to the effects on oral cavity. Strawberries are one of natural ingredients that currently used to whiten decolorized teeth because it contains ellagic acid and malic acid. On contrary, malic acid is said to be involved in the process of erosion. The aim of this study was to compare tooth enamel hardness after application of dental bleaching agent carbamide peroxide 10% and strawberry gel.Material and Methods: Sample consists of 30 maxillary incisors were divided into 3 groups. The first group as control soaked in aquades. The second treatment group were applied with carbamide peroxide 10%  and a third group applied with strawberry gel. Before and after the sample application, tests were performed to determine the hardness of tooth enamel as measured using Universal Hardness Tester.Results: Based on Friedman test, p-value = 0,000 (p <0,05; significant). This means that there was a significant difference in tooth enamel surface hardness of each group and treatment group using bleaching carbamide peroxide 10% and strawberry gel.Conclusion: There was a decrease on tooth enamel hardness after application of carbamide peroxide 10%  compared strawberry gel.
The role of cow milk in reducing the number of streptococcus mutans Irene E. Rieuwpassa; Donald R. Nahusona; Indah Ramadhani
Journal of Dentomaxillofacial Science Vol. 3 No. 3 (2018): (Available online: 1 December 2018)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (709.247 KB) | DOI: 10.15562/jdmfs.v3i3.695

Abstract

Objective: This research aims to provide an overview of chewing gum casein effect on the number of bacteria streptococcus mutans and profile saliva. Casein is one of the elements in cow milk that has 70-80% of the total protein and function to prevent bacterium and other saliva sticking on the enamel surface of teeth. Consuming free sugar gum after eating can help to remineralise enamel, increase saliva and decrease the number of streptococcus mutans. Mouth work on mastication increases salivary flow rate. Therefore saliva production will increase and will change its acidity (pH).Material and Methods: The research design used was experimental research conducted in two groups where the control group by giving xylitol gum and the second group was given chewing casein gum. A total of 20 participants who match the criteria of research subjects chewed gum for 9 consecutive days and calculated the amount of streptococcus mutans, volume and pH of saliva.Results: The number of streptococcus mutans bacteria experienced a decline mainly in the group 69.60 in the first day to 22.80 in the ninth day. Thus, independent examination was done and p<0.005 was indicated, meaning that there was significant difference of casein gum in terms of decreasing streptococcus mutans bacteria, compared with xylitol gum.Conclusion: Casein gum made from cow milk can reduce the number of streptococcus mutans bacteria.
Co-Authors Al Fikriyani Amani Fajri Khairunnisa Amin., Zalzabila M. Amraida Khusnul Khatimah Amru, Muh Akmal Andi M. Farhan Andi M. Rizal Andi Muhammad Fahruddin Andi Muhammad Irfan M. Amir Andi Zaky Hanifan Ansyari Muis Anwar, Vina Maulydia Arsyad, Muhammad Aryadi Asmawati Asmawati Asmawati Asmawati Astriani Benyamin, Roland Deavid Budiawan, Utami Putri Chanifah T. Maulita Dian Rafika Sari D Donald R. Nahusona Dwi P. Wulansari Dyan Megasari Eddy Heriyanto Habar Ema Yusuf Erwin Gunawan Fadhlan Isnan Makkawaru Fajriani Fatimah Azzahra Febriansyah M. Ashar Fitrian Riksavianti Fransiske Tatengkeng Fuad Husain Akbar Gabriel SA. Matongan Gunawan, Muh. Chaerul Hamka, Dinda Cindrahati Harun Achmad Harun H. Lala Hasanuddin Thahir, Hasanuddin I Wayan Suka Arsana Ika, Khaerunnisa Ilhamsyah, Salsabyla Tamara Putri Indah Ramadhani Izzah, Agnes Dea Ugie Wihdatul J. I. Lisal Karlina Karlina Khatimah, Amraida Khusnul Lalu Novan Maulana Lenni Indriani M. Iqbal Basri Makkawaru, Fadhlan Isnan Marhamah Masruroh, Bihaeri Itsna Maulana, Lalu Novan Megatriani Matandung Megumi, A. Apriliiqa Melinda Herman Meuthia Narissa Azzahra Mochammad Hatta Muh. Chaerul Gunawan Muliaty Yunus N, Nur Syabina Octavia Gamal Nur Ain latjompoh Nurlindah Hamrun Nurul Fitri Nusaly, Tiara Rezqi Megautama Pharadiba Pharadiba Purnamasari, Daranisa Wulan Putri, Nabilah Atikah Rafikah Hasyim Rahmat Rahmat Ramadhan, Bagas Karunia Reski Y. S Rifqiyanti Ismi Risnanda Thamrin Sa, Salsabyila Wahyuni Salamah, Ummi Salsabyila Wahyuni Sa Sasmita Sherly Horax Siti Rachmatiara Sitti Rafiah Soelistia Ramadhani Sri Dian Haerawati St. Rahma Lukman Sumintarti Sumintarti Surianti Alimin Syamsiar Toppo Syamsiar Toppo Uleng Bahrun Ummi Salmiah Sari Wahyu Aji Ramadhan Wahyuningrat, Mitha Wijaya Yu-Ri Kim Zakiyah, A. Fadhilah Putri Zalzabila M. Amin