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All Journal Musamus Fisheries and Marine Journal Jurnal Kolaboratif Sains INDONESIAN JOURNAL OF URBAN AND ENVIRONMENTAL TECHNOLOGY JURNAL RISET DIWA BAHARI Jurnal Perikanan Indonesian Journal of Coastal and Marine Studies Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) MARFIG: Journal of Fisheries and Marine Resource Management
Tri Widayati Putri
Department Of Fishery Technology, Institute Of Technology And Business Maritime Balik Diwa, Makassar, South Sulawesi, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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ANALYSIS OF AMINO ACID PROFILE OF ANCHOR SAUCE (Stolephorus sp.) WITH THE ADDITION OF PAPAIN ENZYME Ariansyah Ariansyah; Mutemainna Karim; Tri Widayati Putri; Zul Khairiyah
Jurnal Riset Diwa Bahari (JRDB) Volume 4, Issue 1, 2026
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Institut Teknologi dan Bisnis Maritim Balik Diwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63249/jrdb.v4i1.133

Abstract

Kecap ikan teri merupakan produk fermentasi yang kaya protein sehingga berpotensi menghasilkan kandungan asam amino yang tinggi. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan enzim papain terhadap total asam amino serta mutu organoleptik kecap ikan teri dan membandingkannya dengan kecap tanpa enzim. Metode penelitian menggunakan dua perlakuan, yaitu A (0g enzim papain) sebagai kontrol dan B (10g enzim papain). Analisis komposisi asam amino dilakukan menggunakan kromatografi cair kinerja tinggi (HPLC), sedangkan evaluasi organoleptik dilakukan melalui uji hedonik. Data diolah dengan uji non-parametrik menggunakan Mann-Whitney U. Hasil analisis menunjukkan bahwa total asam amino sampel A adalah 19.49% w/w, sedangkan sampel B adalah 11.54% w/w, yang menunjukkan rendahnya kadar asam amino akibat penambahan enzim papain. Hasil uji organoleptik menunjukkan bahwa penambahan enzim papain menurunkan nilai rasa pada sampel B 2.6 (cukup suka) dan nilai warna pada sampel B 3.1 (cukup suka), sedangkan sampel tanpa penambahan enzim papain meningkatkan nilai rasa pada sampel A 2.7 (cukup suka) dan nilai warna pada sampel A 3.3 (cukup suka), sedangkan sampel tanpa penambahan enzim papain menunjukkan penurunan nilai bau pada sampel A 2.5 (kurang suka) dan nilai tekstur pada sampel A 2.8 (cukup suka), sedangkan pada sampel dengan penambahan enzim papain menunjukkan peningkatan nilai bau pada sampel B 2.6 (cukup suka) dan nilai tekstur pada sampel B 3.0 (cukup suka). Hasil uji Mann-Whitney U menunjukkan bahwa penambahan enzim papain berpengaruh negatif terhadap asam amino dan nilai organoleptic rasa dan warna kecap ikan teri.
ANALYSIS OF THE DEGRADABILITY OF AZO DYES AND MICROBES BIOPLASTICS FROM CELLULOSE Kappaphycus alvarezii Zhaleha Zhaleha; Tri Widayati Putri; Awaluddin Awaluddin; Zul Khairiyah
Jurnal Riset Diwa Bahari (JRDB) Volume 4, Issue 1, 2026
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Institut Teknologi dan Bisnis Maritim Balik Diwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63249/jrdb.v4i1.136

Abstract

The increasing accumulation of non-biodegradable plastic waste has encouraged the development of environmentally friendly alternatives derived from renewable natural resources. One promising material is cellulose extracted from the red seaweed Kappaphycus alvarezii, which possesses favorable characteristics for bioplastic production. This study aimed to evaluate the degradability of azo dyes and microbial activity in bioplastics produced from K. alvarezii cellulose with different cellulose-to-cassava starch ratios. The research was conducted from July to October 2025 using five formulations: F1 (5:5), F2 (8:2), F3 (2:8), F4 (0:10), and F5 (10:0). Cellulose extraction was performed through alkaline and acid hydrolysis methods, while bioplastic films were produced through solution casting with glycerol as a plasticizer. Azo dye degradation was analyzed using UV–Visible spectrophotometry, whereas microbial activity was evaluated through Total Plate Count (TPC) analysis. The UV–Vis results revealed no characteristic absorption peaks of azo dyes in the 300–500 nm wavelength range, indicating the absence or successful degradation of azo chromophores in all formulations. Cellulose-rich formulations exhibited higher optical transparency and greater film homogeneity than starch-dominated formulations. TPC values ranged from 0 to 420 CFU g⁻¹ and remained substantially below the maximum limit established by SNI 7388:2009 (4,400 CFU g⁻¹), confirming excellent microbiological quality and product safety. Statistical analysis using the Kruskal–Wallis test showed no significant differences among formulations (p > 0.05). The combined findings demonstrate that cellulose-based bioplastics derived from Kappaphycus alvarezii possess good biodegradation potential, are free from detectable azo dye contamination, and maintain low microbial contamination levels. Therefore, these bioplastics have strong potential as sustainable alternatives to conventional petroleum-based plastics for food packaging, cosmetic packaging, and other environmentally sensitive applications
Co-Authors A Virati Syamda Abdul Karim Abdul Wahid Wahab Adriani Adriani Agus Darmawan Andi Fahri Faisal Angreni, Husni Annisa Wahdani Arfiani Nur Ariansyah Ariansyah Arie Hijriyah Aryanti Susilowati Audrey Zahra Awaluddin Awaluddin Bismirahma Abbas Dafiuddin Salim Eid Abdalrazaq Fadliah Fadliah Fadliah, Fadliah Fadly Yashari Soumena Ghanima Yasmaniar Hasri Hasri Iin Octaviana Hutagaol Ilham Indah Raya Indah Raya Jawiana Saokani Sofyan Jawiana Saokani Sofyan Jawiana Saokani Sofyan Sofyan Mardiana Muhammad Burhannudinnur Muhammad Ikbal Mutemainna Karim Nur Fajriani Nursida Nurul Khumairah Nurul Safirahmawadda Paulina Taba Ridha Husla Riskawati Riskawati Samsuddin Shivaa Prasad Kollur Subandrio Sufyaningsi, Ulfa Sukmatica Slamet Sulistiani Jarre Syahruddin Kasim Wiwin Anggriani Zhaleha Zhaleha Zul Khairiyah