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ANALYSIS OF AMINO ACID PROFILE OF ANCHOR SAUCE (Stolephorus sp.) WITH THE ADDITION OF PAPAIN ENZYME Ariansyah Ariansyah; Mutemainna Karim; Tri Widayati Putri; Zul Khairiyah
Jurnal Riset Diwa Bahari (JRDB) Volume 4, Issue 1, 2026
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Institut Teknologi dan Bisnis Maritim Balik Diwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63249/jrdb.v4i1.133

Abstract

Kecap ikan teri merupakan produk fermentasi yang kaya protein sehingga berpotensi menghasilkan kandungan asam amino yang tinggi. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan enzim papain terhadap total asam amino serta mutu organoleptik kecap ikan teri dan membandingkannya dengan kecap tanpa enzim. Metode penelitian menggunakan dua perlakuan, yaitu A (0g enzim papain) sebagai kontrol dan B (10g enzim papain). Analisis komposisi asam amino dilakukan menggunakan kromatografi cair kinerja tinggi (HPLC), sedangkan evaluasi organoleptik dilakukan melalui uji hedonik. Data diolah dengan uji non-parametrik menggunakan Mann-Whitney U. Hasil analisis menunjukkan bahwa total asam amino sampel A adalah 19.49% w/w, sedangkan sampel B adalah 11.54% w/w, yang menunjukkan rendahnya kadar asam amino akibat penambahan enzim papain. Hasil uji organoleptik menunjukkan bahwa penambahan enzim papain menurunkan nilai rasa pada sampel B 2.6 (cukup suka) dan nilai warna pada sampel B 3.1 (cukup suka), sedangkan sampel tanpa penambahan enzim papain meningkatkan nilai rasa pada sampel A 2.7 (cukup suka) dan nilai warna pada sampel A 3.3 (cukup suka), sedangkan sampel tanpa penambahan enzim papain menunjukkan penurunan nilai bau pada sampel A 2.5 (kurang suka) dan nilai tekstur pada sampel A 2.8 (cukup suka), sedangkan pada sampel dengan penambahan enzim papain menunjukkan peningkatan nilai bau pada sampel B 2.6 (cukup suka) dan nilai tekstur pada sampel B 3.0 (cukup suka). Hasil uji Mann-Whitney U menunjukkan bahwa penambahan enzim papain berpengaruh negatif terhadap asam amino dan nilai organoleptic rasa dan warna kecap ikan teri.
ANALYSIS OF THE DEGRADABILITY OF AZO DYES AND MICROBES BIOPLASTICS FROM CELLULOSE Kappaphycus alvarezii Zhaleha Zhaleha; Tri Widayati Putri; Awaluddin Awaluddin; Zul Khairiyah
Jurnal Riset Diwa Bahari (JRDB) Volume 4, Issue 1, 2026
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Institut Teknologi dan Bisnis Maritim Balik Diwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63249/jrdb.v4i1.136

Abstract

The increasing accumulation of non-biodegradable plastic waste has encouraged the development of environmentally friendly alternatives derived from renewable natural resources. One promising material is cellulose extracted from the red seaweed Kappaphycus alvarezii, which possesses favorable characteristics for bioplastic production. This study aimed to evaluate the degradability of azo dyes and microbial activity in bioplastics produced from K. alvarezii cellulose with different cellulose-to-cassava starch ratios. The research was conducted from July to October 2025 using five formulations: F1 (5:5), F2 (8:2), F3 (2:8), F4 (0:10), and F5 (10:0). Cellulose extraction was performed through alkaline and acid hydrolysis methods, while bioplastic films were produced through solution casting with glycerol as a plasticizer. Azo dye degradation was analyzed using UV–Visible spectrophotometry, whereas microbial activity was evaluated through Total Plate Count (TPC) analysis. The UV–Vis results revealed no characteristic absorption peaks of azo dyes in the 300–500 nm wavelength range, indicating the absence or successful degradation of azo chromophores in all formulations. Cellulose-rich formulations exhibited higher optical transparency and greater film homogeneity than starch-dominated formulations. TPC values ranged from 0 to 420 CFU g⁻¹ and remained substantially below the maximum limit established by SNI 7388:2009 (4,400 CFU g⁻¹), confirming excellent microbiological quality and product safety. Statistical analysis using the Kruskal–Wallis test showed no significant differences among formulations (p > 0.05). The combined findings demonstrate that cellulose-based bioplastics derived from Kappaphycus alvarezii possess good biodegradation potential, are free from detectable azo dye contamination, and maintain low microbial contamination levels. Therefore, these bioplastics have strong potential as sustainable alternatives to conventional petroleum-based plastics for food packaging, cosmetic packaging, and other environmentally sensitive applications
Uji Mikrobakteri dan Iritasi Sabun Padat Rumput Laut Gracilaria Verrucosa Nurmaida Nurmaida; Harianti Harianti; Tri Widayati Putri
Jurnal Riset Diwa Bahari (JRDB) Volume 1, Issue 2, 2023
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Institut Teknologi dan Bisnis Maritim Balik Diwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63249/jrdb.v1i2.12

Abstract

This study aims to determine the irritant properties of human skin to Gracilaria verrucosa solid soap preparations and organoleptic values. the method used was a pre-experimental design type one group pretestposttest and the data were described descriptively. The results showed that ALT and pH were highest in the control sample with values of 1.9 x 104 colonies/gram and 9.2, respectively. The highest organoleptic value is soap preparation without the addition of Gracilaria verrucosa with a value of 3.2 because it has an attractive appearance with a shiny clear colour. The conclusion of the results showed that the preparation of Gracilaria verrucosa solid soap with the composition of base soap, Gracilaria verrucosa, and essential oil did not irritate the skin of volunteers and organoleptically, the value of the level of Panelists' liking for Gracilaria verrucosa solid soap preparations was highest in soap preparations without the addition of Gracilaria verrucosa compared to soap preparations with the addition of Gracilaria verrucosa. soap preparations with the addition of Gracilaria verrucosa.
Karasteristik Serbuk Buah Mangrove Rhizophora sp. Sri Wulandari; Ziana Karmila; Tri Widayati Putri; Zul Khairiyah
Jurnal Riset Diwa Bahari (JRDB) Volume 1, Issue 2, 2023
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Institut Teknologi dan Bisnis Maritim Balik Diwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63249/jrdb.v1i2.17

Abstract

Mangroves have various roles for human life, one of which is as an alternative food ingredient, namely coffee raw material. Mangrove propagules, especially the fruit, can be processed and produce powders that can be brewed like coffee in general so this study aims to analyze the characteristics of mangrove fruit powder by calculating ash content, water content, caffeine, followed by sensory and hedonic testing of Rhizophora sp. This study used a laboratory-scale quantitative method followed by panelists' assessment of two different samples. Sample one is Rhizophora sp. mangrove fruit powder, while sample two is a mixture of Rhizophora sp. mangrove fruit powder with the addition of Arabica coffee beans. The test results of ash content, moisture content, caffeine, sensory, and hedonic testing respectively showed values of 2.48 and 1.49; 2.94 and 3.03; 1.36 and 0.92; 23.3 and 25.1; and 27.1 and 28.3. So it can be concluded that the water content and ash content of mangrove fruit powder with the addition of arabica coffee beans are higher than mangrove fruit powder. Although the caffeine content of mangrove fruit powder is higher than those that get additional arabica coffee. Mangrove fruit powder with the addition of Arabica coffee beans has a higher value in sensory and hedonic testing, panellists prefer and feel more satisfied when consuming mangrove fruit powder with the addition of Arabica coffee.
Analisis Kimia Telur Ikan Tuna Mata Besar (Thunnus obesus) dari Berbagai Tingkat Kematangan Gonad Tri Widayati Putri; Nurulhikmah Kamaruddin; Zul Khairiyah
Jurnal Riset Diwa Bahari (JRDB) Volume 3, Issue 1, 2025
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Institut Teknologi dan Bisnis Maritim Balik Diwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63249/jrdb.v3i1.39

Abstract

Bigeye tuna (Thunnus obesus) is a type of large pelagic fish belonging to the Scombriade family that can spawn repeatedly during the spawning season. Bigeye tuna eggs have several phases of gonad maturity, including gonad maturity level IV and gonad maturity level V. This study aims to determine the chemical composition and mineral composition of bigeye tuna eggs (Thunnus obesus) from two levels of gonad maturity, namely gonad maturity level IV and gonad maturity level V. The parameters analyzed were water content, ash content, protein content, fat content, iron and zinc with three repetitions for each parameter. This study was conducted from June to August 2024. Sampling was carried out at two different locations with samples in the form of bigeye tuna eggs (Thunnus obesus). The results of the study showed that bigeye tuna eggs from gonad maturity level IV had a water content of 69.94%, ash content of 4.04%, protein content of 20.43%, fat content of 0.94%, iron content of 34.42% and zinc content of 142.58%. While the gonad maturity level V had a water content of 72.01%, ash content of 3.22%, protein content of 19.82%, fat content of 1.55%, iron content of 14.83% and zinc content of 172.50%. Based on the statistical results, it showed that bigeye tuna eggs had significant differences (significantly different) in water content, ash content, protein, fat, iron and zinc at two gonad maturity levels IV and gonad maturity level V.
Karakteristik Mekanik Selulosa Dari Rumput Laut Kappaphycus alvarezii di Kabupaten Takalar Tri Widayati Putri; Reski Ardini Yusra; Awaluddin Awaluddin
Jurnal Riset Diwa Bahari (JRDB) Volume 3, Issue 1, 2025
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Institut Teknologi dan Bisnis Maritim Balik Diwa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.63249/jrdb.v3i1.71

Abstract

Kandungan selulosa dan karakteristik mekanik selulosa dalam menentukan kelayakan rumput laut sebagai bahan baku biomaterial ramah lingkungan, selain sifat fisikokimia selulosa diperlukan untuk menunjang pemanfaatan limbah rumput laut secara optimal. Sehubungan dengan hal tersebut, penelitian ini bertujuan untuk menganalisis kadar dan karakteristik mekanik selulosa dari rumput laut Kappaphycus alvarezii yang berasal dari Kabupaten Takalar. Penelitian ini dilaksanakan dari bulan September hingga Januari 2025 di Makassar, dengan menggunakan pendekatan deskriptif melalui metode kualitatif. Hasil penelitian menunjukkan bahwa kadar selulosa meningkat dari 12,31% pada sampel mentah menjadi 20,04% setelah proses ekstraksi. Analisis FTIR mengidentifikasi gugus fungsi khas selulosa seperti –OH, C=O, dan C–O–C, sementara SEM menunjukkan perubahan morfologi menjadi lebih berpori dan homogen setelah perlakuan kimia. Temuan ini mengindikasikan bahwa Kappaphycus alvarezii berpotensi tinggi sebagai sumber bahan selulosa yang dapat dikembangkan untuk keperluan bioplastik atau biomaterial lainnya.