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All Journal Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Pontianak Nutrition Journal (PNJ) Journal of Community Empowerment for Health International Journal of Social Science, Educational, Economics, Agriculture Research, and Technology (IJSET) Pharmademica: Jurnal Kefarmasian dan Gizi Svasta Harena: Jurnal Ilmiah Gizi Jurnal Abdi Inovatif : Pengabdian kepada Masyarakat Pontianak Nutrition Journal (PNJ) Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS)
Arindra Nirbaya
Universitas Negeri Malang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Other

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ANTIBACTERIAL ACTIVITY OF USED COOKING OIL-BASED LIQUID SOAP AGAINST ESCHERICHIA COLI AND STAPHYLOCOCCUS AUREUS Rokhmad Fajar Setiyo; Zahra Anggita Pratiwi; Arindra Nirbaya; Puji Putri Isnaini
International Journal of Social Science, Educational, Economics, Agriculture Research and Technology (IJSET) Vol. 5 No. 10 (2026): SEPTEMBER
Publisher : RADJA PUBLIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.23135149

Abstract

Used cooking oil can be turned into liquid soap by saponification, but soap made only from oil and KOH contains no added antibacterial agent. This study aimed to analyze the antibacterial activity of used cooking oil-based liquid soap with added lemongrass (Cymbopogon citratus) against Escherichia coli and Staphylococcus aureus. A laboratory experiment with a completely randomized design used four formulations: F0 (no lemongrass), F1 (1%), F2 (2%), and F3 (3%). Activity was tested by well diffusion, with chloramphenicol and distilled water as positive and negative controls and pure lemongrass essential oil as a comparison; inhibition zones (mm) were measured in triplicate. All formulations inhibited E. coli, with mean zones increasing from 4.95 ± 0.80 mm (F0, weak) to 6.13 ± 0.5 mm (F3, moderate), indicating a dose-response pattern. Against S. aureus, all formulations showed only weak activity (3.03–3.83 mm) without a clear concentration trend. The soap base alone showed some inhibition, and responses differed between bacteria, possibly because of interactions between surfactants and lemongrass essential oil in the soap matrix.
Co-Authors Afinatul Jannah Agung Witjoro Ajeng Daniarsih Aprinia Dian Nurhayati Aprinia Dian Nurhayati Cassandra Permata Nusa Choirunisa, Ratnani Cindy Elysia Elora Dewi Ima Fatmawati Dwi Oktavia Wahyuningsih Prastiwi Fatmawati, Dewi Ima Hanna Eklesia Manik Kholidah Nur Aini Lelitawati, Mardiana Muhammad Andry Prio Utomo Noer Sholeh Krisna Puji Putri Isnaini Rizka Romadona Rokhmad Fajar Setiyo Rudianto, Rony Setyaningrum, Yahmi Siti Imroatul Maslikah Sri Hapsari Suhartono Putri Sri Rahayu Lestari SUPRIYONO Vivi Viranda Sania Putri Wildan Agatha Saputra Yahmi Ira Setyaningrum Yahmi Ira Setyaningrum Yahmi Ira Setyaningrum Yunita Rakhmawati Zahra Alifia Yuwaida Zahra Anggita Pratiwi