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All Journal Jurnal Bahan Alam Terbarukan Sainteknol : Jurnal Sains dan Teknologi Majalah Farmaseutik Jurnal Farmasi dan Sains Indonesia (JFSI) Journal of Science and Technology Research for Pharmacy Media Farmasi Indonesia Parapemikir Jurnal Ilmiah Farmasi
Buanasari, Buanasari
Akademi Farmasi Nusaputera
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Other

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EXTRACTION OF PHENOLIC COMPOUNDS FROM PETAI LEAVES (PARKIA SPECIOSA HASSK.) USING MICROWAVE AND ULTRASOUND ASSISTED METHODS Buanasari, Buanasari; Eden, Willy Tirza; Sholichah, Ayu Ina
Jurnal Bahan Alam Terbarukan Vol 6, No 1 (2017): June 2017 [Nationally Accredited]
Publisher : Semarang State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v6i1.7793

Abstract

The antioxidant has an activity to neutralize free radical compound that the body needs to avoid damage cells and tissues. Phenolic is one of the compounds that have an antioxidant activity. The influences of ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE) conditions on phenolic compounds of Parkia speciosa Hassk. leaves were investigated. The effects of temperature (40°C, 50°C, 60°C and 70°C), time (10, 30 and 50 minutes) and material-solvent ratio (1:10, 1:13, 1:15 ) were evaluated based on the yield, total phenolic content (TPC) and antioxidant activity. The result showed that the highest yield (15.82%) was obtained at 1:15 (w/w) of material-solvent ratio, 50°C of temperature and 50 minutes of extraction time for MAE. The highest yield of UAE is 15.53% that sample was obtained at 1:13 (w/w) of material-solvent ratio, 60°C of optimal temperature and 30 minutes extraction time. The highest IC50 of UAE method extract was 52.55 ppm, while the extract obtained using MAE method was 50.44 ppm. UAE is more stable at higher temperatures. Time and solvent which was used more efficient than MAE. Extract of petai leaves (Parkia speciosa Hassk.) were very potential to be used as a source of natural antioxidants because they have IC50 values from 41.39 to 66.00 ppm. Its antioxidants capacity is ranged from strong to very strong capacity.
THE PROCESS OF ENHANCED OIL RECOVERY (EOR)USING ALKALINE SURFACTANT (AS) FLOODING ON SUKOWATI CRUDE OIL: A COMPARISON STUDY OF MIXED SURFACTANT Buanasari, Buanasari; Pramudono, Bambang
Sainteknol : Jurnal Sains dan Teknologi Vol 15, No 1 (2017): Juni 2017
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v15i1.12386

Abstract

We investigated six mixed surfactants for effectiveness in enhanced oil recovery (EOR). The selected surfactant formulation were tested for EOR using coreflood tests beneath sandstones. There are four objectives in this research. The first objective would examine synergism of surfactant mixtures, i.e., MES-SLS; MES- LAS; MES-DEA; MES-Tween 80; MES-BAC; and BAC-Tween 80. The next study examines the optimal ratio of the best surfactant mixture. The next step is to determine the best alkali between NaOH and Na2CO3. The final objective would determine the optimal concentration of alkaline in this formulation. Surfactant formulation would use brine 5000 ppm. This formulation injected with a flow rate of 10 ml/s at temperature of 70oC for 3 minutes beneath sandstones. Oil recovery results and the IFT value are observed response in this study. This research has found MES-DEA (½: 1) and alkali sodium carbonate 1% wt/v as the best formulations. This formulation has given 32,88% wt oil recovery and IFT value of 3,29 x 10-1 mN/m.
Potential of Chitosan From Local Crab (Portunus Pelagicus) to Enhance Storability of Musa Paradisiaca L. Buanasari, Buanasari; Sugiyo, Warlan; Fitriani, Nur; Suryaningsih, Suryaningsih
Jurnal Bahan Alam Terbarukan Vol 8, No 1 (2019): June 2019 [Nationally Accredited - Sinta 2]
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v8i1.16423

Abstract

Potential waste of local crab carapace (Portunus pelagicus) as a source of chitosan as an active layer that can protect bananas has been studied. The process in this study consists of three stages. The first stage was the isolation of chitin through deproteinization process using 2.0 N NaOH solution with a ratio of 1:6 w/v and demineralization process using 1.5 N HCl solution with a ratio of 1:12 w/v. The second stage is the deacetylation stage using 50% NaOH solution with a ratio of 1:20 w/v. Fourier Transform Infra-Red (FTIR) Spectroscopy is used to determine the degree of deacetylation. The third stage is the banana coating application using chitin solution to determine the shelf life of bananas with variations in levels of 2, 2.5, 3 and 3,5 % w/v by immersion method for one hour. It was found that carapace crab, a part that was underutilized from crab, gave rise to chitin deacetylation with a deacetylation rate of 62.11%; pH 8.9 and water content of 7.677%. Chitosan-based coatings are applied to fresh bananas and are found to increase fruit firmness, and inhibit browning. The results show that chitosan-coated bananas have a longer storage time. The application of chitin deacetylated (chitosan) as fruit banana coater found that higher coater levels extend the shelf life of bananas with the best coater content is 3% b/v. It results in a shelf life of bananas for up to 12 days, this is longer than bananas without chitosan layer which only has a shelf life of four days. Increased coating rates have a positive effect on the shelf life of bananas. This study shows that waste from carapace crabs can be used to form active layers that can preserve fruit.
EXTRACTION OF PHENOLIC COMPOUNDS FROM PETAI LEAVES (PARKIA SPECIOSA HASSK.) USING MICROWAVE AND ULTRASOUND ASSISTED METHODS Buanasari, Buanasari; Eden, Willy Tirza; Sholichah, Ayu Ina
Jurnal Bahan Alam Terbarukan Vol 6, No 1 (2017): June 2017 [Nationally Accredited]
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v6i1.7793

Abstract

The antioxidant has an activity to neutralize free radical compound that the body needs to avoid damage cells and tissues. Phenolic is one of the compounds that have an antioxidant activity. The influences of ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE) conditions on phenolic compounds of Parkia speciosa Hassk. leaves were investigated. The effects of temperature (40°C, 50°C, 60°C and 70°C), time (10, 30 and 50 minutes) and material-solvent ratio (1:10, 1:13, 1:15 ) were evaluated based on the yield, total phenolic content (TPC) and antioxidant activity. The result showed that the highest yield (15.82%) was obtained at 1:15 (w/w) of material-solvent ratio, 50°C of temperature and 50 minutes of extraction time for MAE. The highest yield of UAE is 15.53% that sample was obtained at 1:13 (w/w) of material-solvent ratio, 60°C of optimal temperature and 30 minutes extraction time. The highest IC50 of UAE method extract was 52.55 ppm, while the extract obtained using MAE method was 50.44 ppm. UAE is more stable at higher temperatures. Time and solvent which was used more efficient than MAE. Extract of petai leaves (Parkia speciosa Hassk.) were very potential to be used as a source of natural antioxidants because they have IC50 values from 41.39 to 66.00 ppm. Its antioxidants capacity is ranged from strong to very strong capacity.
POTENSI METODE ULTRASONIC-ASSISTED EXTRACTION (UAE) DALAM MENGEKSTRAK SENYAWA AKTIF DARI BAHAN ALAM Buanasari; Yahya Febrianto; Cholifah; Abdul Chakim
Jurnal Farmasi & Sains Indonesia Vol 2 No 1 (2019)
Publisher : LPPM Sekolah Tinggi Ilmu Farmasi Nusaputera

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Teknik ekstraksi konvensional yang digunakan selama bertahun-tahun yang lalu membutuhkan banyak waktu dan pelarut, sehingga memiliki tingkat efisiensi yang rendah. Telah ditemukan beberapa metode alternatif baru, seperti ekstraksi fluida superkritik, ekstraksi solven aselerasi, ekstraksi ultrasonik dan microwave untuk mengekstrak senyawa fitokimia dari tanaman. Sejumlah hasil penelitian menunjukkan bahwa penerapan ultrasonik mampu mengekstrak senyawa fitokimia, seperti alkaloid, flavonoid, polisakarida, protein dan minyak esensial dari berbagai bagian tanaman dan bibit tanaman. Penelitian ini bertujuan untuk mengkaji potensi metode Ultrasonik-assited extraction (UAE) untuk beberapa tanaman (daun jambu air (Syzygium samarangense) dan daun melinjo (Gnetum gnemon L.) ). Ekstraksi dilkukan dengan memvariasikan rasio padatan:pelarut (1: 5, -1:25 g/mL), waktu ekstraksi (10-50 menit) dan suhu (10-50ºC) serta kondisi tetap pelarut etanol 70%V dan ultrasonik daya 200 W dan frekuensi 14 kHz. Respon yang diamati adalah kandungan total flavonoid dan aktivitas antioksidan dengan metode DPPH (2,2-difenil-1-pikrillhidrazil)-SA. Hasil menunjukkan metode ini sangat efektif untuk mengekstrak kandungan zat aktif tanaman. Aktifitas antioksidan dan kandungan total flavonoid dari daun jambu air didapatkan pada kondisi operasi rasio padatan:pelarut (1:10 g/mL), waktu ekstraksi (30 menit) dan suhu (40ºC) yaitu 86,17 ± 0,00 % dan 4,80 ± 0,01 %B. Sedangkan untuk daun melinjo pada kondisi operasi rasio padatan:pelarut (1:10 g/mL), waktu ekstraksi (10 menit) dan suhu (50ºC) yaitu 55.29± 0,02% dan 3,99 ± 0,01 %B. Kebutuhan solven didapatkan rata-rata 1:10 g/mL, waktu 10-30 menit, dan suhu 40-50 ºC sudah mampu memberikan hasil optimal. Metode UAE ini sangat efektif dari segi waktu dan kebutuhan pelarut dalam mengekstrak zat aktif dari tanaman.
Effect of Ultrasonic Assisted on The Degree of Deacetylation of Chitosan Extracted from Portunus Pelagicus Buanasari, Buanasari; Sugiyo, Warlan; Rustaman, Heri
Jurnal Bahan Alam Terbarukan Vol 10, No 1 (2021): June 2021 [Nationally Accredited - SINTA 2]
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v10i1.27648

Abstract

The technology for extracting chitin from shell and other materials needs to be continuously improved, including its conversion to chitosan. Chitosan is a biocompatible polymer, biodegradable, non-toxic, water-soluble at pH below 6.5, and it has protonated amino groups. The benefits of chitosan in industry, food and medicine make it necessary to fully study an efficient chitosan synthesis method and the results can be applied on an industrial scale. This study examined the effect of ultrasonic-assisted in increasing the degree of deacetylation of chitosan produced from Portunus pelagicus shell waste. The production process of chitosan goes through the stages of deproteination, demineralization and deacetylation. All these steps are ultrasound assisted processes with a frequency of 40 kHz through a digital ultrasonic cleaner. Ultrasonic-assisted chitin and chitosan were examined using FTIR spectrometry. The results showed that the ultrasonic method was able to increase the deacetylation degree of chitin with a value of 68.45±0.11% compared to 62.52±0.08% without ultrasonic. Application of ultrasonic assisted deacetylation gave a deacetylation degree of 85.35 ± 0.20%, higher than without ultrasonic 80.24 ± 0.19%.  Physically, ultrasonic-assisted chitosan is smoother and brighter in color. The ultrasonic-assisted chitosan manufacturing method could increase the deacetylation degree and produce high grade chitosan.
Effect of Variation Conditions of The Extraction Process of Morinda Citrifolia L Leaves Using Ultrasound-Assisted Extraction Method (Uae) Buanasari, Buanasari; Dhamayanti, Sonia Murniana Sri; Suryaningsih, Suryaningsih
Journal of Science and Technology Research for Pharmacy Vol 1 No 1 (2021)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jstrp.v1i1.44348

Abstract

Background: Morinda citrifolia L. is widely used as traditional medicine for various diseases. The benefits of noni are studied from the seeds, fruit, leaves and root bark. This leaf active compound is rich in flavonoids, so an effective extraction process is needed to extract it. Conventional extraction generally takes a long time and involves a thermal process that can damage the compound, so it requires extraction with the latest methods, one of which is the use of ultrasonic waves. Aim: This study aims to examine the effect of variations in extraction process conditions on present yield, DPPH scavenging activity, flavonoid content and phenol content of Morinda citrifolia L leaves by varying the solids-solvent ratio (1:10, 1:20, 1:30, dan 1:40 g/mL), and extraction temperature (25, 35, 45, and 55ºC). Method: The process uses the ultrasonic assisted extraction method with 50 %V ethanol for 60 minutes. Result: The highest yield was obtained in the extraction with a solids-solvent ratio of 1:40 g/mL, at an extraction temperature of 55ºC, which was 32.29±0.066%. The highest flavonoid content (173.41±0.615 mg quercetin equivalent/g extract), phenol content (197.00±0.148 mg gallic acid equivalents/g extract) and DPPH scavenging activity (97.65±0.912%) was obtained in the extraction with a solids-solvent ratio of 1:30 g/mL, at an extraction temperature of 25ºC. The best extract measured antioxidant activity and IC50 values obtained with 23.21 µg/mL. Conclusion: The use of the ultrasonic assisted extraction method by selecting the optimal operating conditions greatly increases the amount of active compound uptake required.
Effect of Ultrasonic Assisted on The Degree of Deacetylation of Chitosan Extracted from Portunus Pelagicus Buanasari, Buanasari; Sugiyo, Warlan; Rustaman, Heri
Jurnal Bahan Alam Terbarukan Vol 10, No 1 (2021): June 2021 [Nationally Accredited - Sinta 2]
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v10i1.27648

Abstract

The technology for extracting chitin from shell and other materials needs to be continuously improved, including its conversion to chitosan. Chitosan is a biocompatible polymer, biodegradable, non-toxic, water-soluble at pH below 6.5, and it has protonated amino groups. The benefits of chitosan in industry, food and medicine make it necessary to fully study an efficient chitosan synthesis method and the results can be applied on an industrial scale. This study examined the effect of ultrasonic-assisted in increasing the degree of deacetylation of chitosan produced from Portunus pelagicus shell waste. The production process of chitosan goes through the stages of deproteination, demineralization and deacetylation. All these steps are ultrasound assisted processes with a frequency of 40 kHz through a digital ultrasonic cleaner. Ultrasonic-assisted chitin and chitosan were examined using FTIR spectrometry. The results showed that the ultrasonic method was able to increase the deacetylation degree of chitin with a value of 68.45±0.11% compared to 62.52±0.08% without ultrasonic. Application of ultrasonic assisted deacetylation gave a deacetylation degree of 85.35 ± 0.20%, higher than without ultrasonic 80.24 ± 0.19%.  Physically, ultrasonic-assisted chitosan is smoother and brighter in color. The ultrasonic-assisted chitosan manufacturing method could increase the deacetylation degree and produce high grade chitosan.
THE PROCESS OF ENHANCED OIL RECOVERY (EOR)USING ALKALINE SURFACTANT (AS) FLOODING ON SUKOWATI CRUDE OIL: A COMPARISON STUDY OF MIXED SURFACTANT Buanasari, Buanasari; Pramudono, Bambang
Sainteknol : Jurnal Sains dan Teknologi Vol 15, No 1 (2017): June 2017
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v15i1.12386

Abstract

We investigated six mixed surfactants for effectiveness in enhanced oil recovery (EOR). The selected surfactant formulation were tested for EOR using coreflood tests beneath sandstones. There are four objectives in this research. The first objective would examine synergism of surfactant mixtures, i.e., MES-SLS; MES- LAS; MES-DEA; MES-Tween 80; MES-BAC; and BAC-Tween 80. The next study examines the optimal ratio of the best surfactant mixture. The next step is to determine the best alkali between NaOH and Na2CO3. The final objective would determine the optimal concentration of alkaline in this formulation. Surfactant formulation would use brine 5000 ppm. This formulation injected with a flow rate of 10 ml/s at temperature of 70oC for 3 minutes beneath sandstones. Oil recovery results and the IFT value are observed response in this study. This research has found MES-DEA (½: 1) and alkali sodium carbonate 1% wt/v as the best formulations. This formulation has given 32,88% wt oil recovery and IFT value of 3,29 x 10-1 mN/m.
Blood Glucose Level Lowering Effect Of Alkaline Reduced Water (ARW) In Streptozotocin-Induced Diabetic Rats Poppy Diah Palupi; Metrikana Novembrina; Buanasari Buanasari; Fita Rahmawati; Tri Murti Andayani
Majalah Farmaseutik Vol 16, No 1 (2020)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.367 KB) | DOI: 10.22146/farmaseutik.v16i1.45050

Abstract

Alkaline Reduced Water (ARW) is beneficial to prevent oxidative stress disease such as diabetes. The aim of the study is to determine the effect of ARW on reducing blood glucose level in diabetic rats induced streptozotocin. Fifteen male Wistar rats were divided into 3 groups. Group 1 (normal) nondiabetic rats, group 2 diabetic rats (negative control) received single intraperitoneal of streptozotocin (65mg/kg), group 3 was diabetic rats treated with ARW 20 mL/kg/day, orally for 28 days. Blood samples were collected through retro-orbital method before and after the experiment. Measured parameter were body weight and blood glucose concentration. Data were analyzed using one-way analysis of variance test followed by Dunnet multiple comparisons post hoc test.  Administration of ARW for 28 days resulted in significant (p< 0.05) lowering of blood glucose. In conclusion, the results show that ARW can lower elevated blood glucose against streptozotocin-induced diabetes in rats. 
Co-Authors -, Shihabuddin Abdul Chakim Arini Chyntia Apriyanti Ayu Ina Sholichah, Ayu Ina Ayu Ina Solichah Badahdah, Nilam Kencana Bambang Pramudono Cholifah Dhamayanti, Sonia Murniana Sri Felisia Bani Ban Fita Rahmawati Metrikana Novembrina NUR FITRIANI PALUPI, Poppy Diah Pramudono, Bambang Rustaman, Heri Sandi Mahesa Yudhantara Sri Suwarni Suryaningsih Suryaningsih Tri Murti Andayani Warlan Sugiyo Warlan Sugiyo Willy Tirza Eden, Willy Tirza Yahya Febrianto