Article Per Year (5 Year)
p-Index From 2020 - 2025
2.862
P-Index
This Author published in this journals
All Journal Planta Tropika AGRARIS: Journal of Agribusiness and Rural Development Research Jurnal Penelitian Pendidikan IPA (JPPIPA) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Sains dan Teknologi Pertanian AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Prosiding Seminar Nasional Program Pengabdian Masyarakat Tropical Plantation Journal Jurnal Ilmiah Multidisiplin Indonesia
Bambang Heri Isnawan
Agrotechnology Department, Faculty Of Agriculture, Universitas Muhammadiyah Yogyakarta, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences

Published : 31 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 31 Documents
Search

 1   2   3   4 
The Effect of Sample Weigh, Incubation Duration And Isolation Methods on The Quality of Phalaeonopsis amabilis root DNA Handayani, Etty; Rineksane, Innaka Ageng; Isnawan, Bambang Heri; Setiawan, Haris Anggita; Manto, Agus
Tropical Plantation Journal Vol 4, No 2 (2025): TROPICAL PLANTATION JOURNAL
Publisher : Akademi Komunitas Perkebunan Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56125/tpj.v4i2.63

Abstract

Orchid Ph. amabilis is one of the plants designated as "Indonesian National Flower" and designated as "Puspa Pesona Indonesia". Orchid Ph. amabilis has a diversity that is not widely known, so that it is necessary to identify it molecularly. DNA isolation is the first step in molecular testing. This study aims to determine the sample weight, incubation time and the appropriate isolation method for DNA isolation from samples of Ph.amabilis orchid roots. In this study, the DNA isolation stage used two experimental methods, namely the CTAB method (Murray and Thompson) and the mini kit. The research method used was a single factor experiment with a combination of sample weight and incubation time consisting of 6 treatments with 3 replications, so 18 experimental units were obtained. The experimental treatment used was from the roots of the orchid Ph. amabilis which has a sample weight of 0.25 g, 0.5 g, and 0.75 g and each sample weight treatment was incubated for 30 minutes and 60 minutes. In addition, DNA isolation using the kit method had a sample weight of 0.25 g, 0.5 g and 0.75 g and an incubation period of 10 minutes. The results showed that the DNA isolation of Ph. amabilis with a sample weight of 0.75g, incubation time of 30 minutes using the CTAB method showed the best treatment in producing DNA with a concentration of 384 ng/µl and a purity of 1.9. This study is expected to provide information on sample weight, incubation time and the appropriate method for DNA isolation of Ph. amabilis orchid roots.
Co-Authors - Hariyono Agung Astuti Aksan, Muhammad Anggi Rama Putra Dewi, Sukuriyati Susilo Dwi Purwana Emmy Hamidah Endri Ridwan Saleh Etty Handayani Gatot Supangkat Gumilar, Aldi Gunawan Budiyanto Handayani, Etty Hariyono Hariyono Hariyono Idum Satia Santi Iffa Nur Adillah Innaka Ageng Rineksane Jamhari Jamhari Khusna, Farida Khusnul Mubarok, Khusnul Lis Noer Aini Lis Noer Aini, Lis Noer Manto, Agus Mubarok, Khusnul Mulyono Nur Rahmawati Purwana, Dwi Ratri Sekarsari Saleh, Endri Ridwan Sekarsari, Ratri Setiawan, Chandra Kurnia Setiawan, Haris Anggita Sriyadi Sriyadi Tasya Putri Timami Nur Septiana Triyono, Triyono Utama, Nafi Ananda Utari, Lilik Zulfa Abida