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BIOSURFAKTAN BAKTERI HIDROKARBONOKLASTIK DALAM PROSES BIOTRANSFORMASI POLYAROMATIC HYDROCARBONS (PAHS) : POTENSI AKSELERASI BIODEGRADASI PAHS Hidayati, Nuning Vita; Syakti, Agung Dhamar; Darmayati, Yeti
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Abstract

Capability of Rhodobacterium bacteraceae in producing biosurfactant were investigated. Biosurfactant has been produced using medium of Mineral Salt Medium with two sources of carbon, which is crude oil (RB-CO) and waste coconut oil (RB-WCO) as carbon sources. Biosurfactant extent was determined by the yield of biosurfactant and the surface tension reduction. Biosurfactant were investigated for their efectiveness on PAHs biodegradation. The results showed that R. bacteraceae can produce biosurfactant, and showed the highest biosurfactant yield (2,791 g/l) when grown on RB-WCO, while we found 2,122 g/l yield on MSM-CO after 72 hours. The best biosurfactant activity was obtained when using waste coconut oil as carbon source, resulted in surface tension reduction up to 29,17 mN/m. Addition of 50 mg of biosurfactant per 200 ml of the mineral salt medium containing 50 ppm PAHs significantly enhanced the PAHs degradation indicating that the organism utilized crude oil as carbon source. Our concluding remarks suggest that biosurfactant from R. bacteraceae when grown on waste coconut oil substrate can be used as an effective agent to be applied on site contaminated with PAHs by enhancing biodegradation rate.
KAPASITAS ASIMILASI BEBAN PENCEMARAN DI PERAIRAN TELUK RIAU Idris, Fadhliyah; Koenawan, Chandra Joei; Jumsurizal, Jumsurizal; Syakti, Agung Dhamar
Dinamika Maritim Vol 6 No 2 (2018): Dinamika Maritim, Vol. 6 No. 2, February 2018
Publisher : Coastal and Marine Resources Research Center, Raja Ali Haji Maritime University, Tanjungpinang, Indonesia

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Abstract

Riau bay, Tanjungpinang City has been subject for domestic wastes contamination due to the high number of population living in the coastal area. This study aims to determine water quality condition including the pollutant load and the level of assimilation capacity in Riau bay waters. Using a survey method by purposive sampling strategy, the research was conducted by analyzing several physic-chemicals parameters. The results showed the measured physical parameters was still below the threshold level stipulating Minister Decree No. No 51 of 2004. Pollutant loads represented by BOD5, TSS  and ammonia were 12,71 mg/L, 239,95  mg/L and 5,33 mg/L respectiely. Our finding showed the assimilation capacity level for BOD5, TSS  and ammonia were 159,26 ton/year, 530,98 ton/year and 0,86 ton/year, respectively.
Isolation, Selection and Identification of Polyaromatic Hydrocarbons (PAHs) Degrading Bacteria from Heavy Oil Waste (HOW)-Contaminated Soil Yani, Mohamad; Charlena, Charlena; Mas?ud, Zainal Alim; Anas, Iswandi; Setiadi, Yadi; Syakti, Agung Dhamar
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.935 KB) | DOI: 10.4308/hjb.27.2.142

Abstract

The heavy oil waste (HOW) containing polyaromatic hydrocarbon (PAHs) is a persistent organic pollutants (POPs) that difficult to degrade. The new PAH degrading consortium was investigated from HOW contaminated soil in North Sumatera of Indonesia. The isolation, selection and identification of polyaromatic hydrocarbon degrading bacteria from soil contaminated by HOW was conducted to solve a bioremediation process. The isolation microbes from soil contaminated by HOW was performed using a minimum ONR7a media and followed on marine agar media for purification purposes. From the performed isolation results, 11 isolates were able to degrade PAHs compounds, such as phenanthrene, dibenzothiophene, or fluorene compounds. They grew at pH range of 4.8-8.2 and performed on emulsification activity in paraffin from 0.150-0.662. Three of them showed the best performance on HOW biodegradation capability and then successfully selected and identified as Salipiger sp., Bacillus altitudinis, and Ochrobactrum anthropi. using 16S rDNA. The HOW biodegradation as TPH-degradation were 38.66%, 59.60%, and 47.16%, respectively. Those isolated bacteria could potentially be as bioremediation agents to develop on bioremediation process for soils contaminated by HOW.
KAPASITAS ASIMILASI BEBAN PENCEMARAN DI PERAIRAN TELUK RIAU Idris, Fadhliyah; Koenawan, Chandra Joei; Jumsurizal, Jumsurizal; Syakti, Agung Dhamar
Dinamika Maritim Vol 6 No 2 (2018): Dinamika Maritim, Vol. 6 No. 2, February 2018
Publisher : Pusat Penelitian Sumberdaya Pesisir dan Laut, Universitas Maritim Raja Ali Haji, Indonesia

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Abstract

Riau bay, Tanjungpinang City has been subject for domestic wastes contamination due to the high number of population living in the coastal area. This study aims to determine water quality condition including the pollutant load and the level of assimilation capacity in Riau bay waters. Using a survey method by purposive sampling strategy, the research was conducted by analyzing several physic-chemicals parameters. The results showed the measured physical parameters was still below the threshold level stipulating Minister Decree No. No 51 of 2004. Pollutant loads represented by BOD5, TSS and ammonia were 12,71 mg/L, 239,95 mg/L and 5,33 mg/L respectiely. Our finding showed the assimilation capacity level for BOD5, TSS and ammonia were 159,26 ton/year, 530,98 ton/year and 0,86 ton/year, respectively.
Isolation, Selection and Identification of Polyaromatic Hydrocarbons (PAHs) Degrading Bacteria from Heavy Oil Waste (HOW)-Contaminated Soil Mohamad Yani; Charlena Charlena; Zainal Alim Mas’ud; Iswandi Anas; Yadi Setiadi; Agung Dhamar Syakti
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.935 KB) | DOI: 10.4308/hjb.27.2.142

Abstract

The heavy oil waste (HOW) containing polyaromatic hydrocarbon (PAHs) is a persistent organic pollutants (POPs) that difficult to degrade. The new PAH degrading consortium was investigated from HOW contaminated soil in North Sumatera of Indonesia. The isolation, selection and identification of polyaromatic hydrocarbon degrading bacteria from soil contaminated by HOW was conducted to solve a bioremediation process. The isolation microbes from soil contaminated by HOW was performed using a minimum ONR7a media and followed on marine agar media for purification purposes. From the performed isolation results, 11 isolates were able to degrade PAHs compounds, such as phenanthrene, dibenzothiophene, or fluorene compounds. They grew at pH range of 4.8-8.2 and performed on emulsification activity in paraffin from 0.150-0.662. Three of them showed the best performance on HOW biodegradation capability and then successfully selected and identified as Salipiger sp., Bacillus altitudinis, and Ochrobactrum anthropi. using 16S rDNA. The HOW biodegradation as TPH-degradation were 38.66%, 59.60%, and 47.16%, respectively. Those isolated bacteria could potentially be as bioremediation agents to develop on bioremediation process for soils contaminated by HOW.
Manajemen Kualitas Air Dengan Teknologi Bioflok: Studi Kasus Pemeliharan Ikan Lele (Clarias Sp.) Nadya Adharani; Kadarwan Soewardi; Agung Dhamar Syakti; Sigid Hariyadi
Jurnal Ilmu Pertanian Indonesia Vol. 21 No. 1 (2016): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.093 KB) | DOI: 10.18343/jipi.21.1.35

Abstract

Biofloc technology is one of the alternative to overcome the problem of water quality of cultivation environment which was adapted from conventional waste management techniques. The purpose of this study was to assess the young catfish culture by applying bioflocs of some consortium products, such as Bacillus megaterium (BM), Supernit (SP), Depok 165 (DP165), Kayajaga (KJ) compared to non biofloc application for improving the water quality. The results of this study and statistical test showed that the lowest concentration of TAN by probiotic KJ was about 2.56 mg L-1, while the control was about 5.47 mg L-1, and the consortium of bacteria gave effect to  TAN value (p<0.05). The Lowest concentration of amonia by probiotic KJ was about 0.0001853 mg L-1, while control was about 0.0003973 mg L-1, and the consortium of bacteria did not give effect to amonia value (p>0.05). The lowest concentration of nitrite by probiotic BM was about 0.065 mg L-1, while the control  was about 0.124 mg L-1, and the consortium of bacteria gave effect to the nitrite value (p<0.05). The lowest concentration of nitrate by probiotic BM was about 1.203 mg L-1, while the control was about 3.437 mg L-1, and the consortium of bacteria gave effect to the nitrate value (p<0.05). Consortium of bacteria gave effect to the COD value (p<0.05), but all bioflocs treatments had an average value of COD higher than the control. The conclusion that the bioflocs aplication was able to improve the water quality shown by decreasing parameter values of TAN concentrations, ammonia, nitrite, and nitrate.
The Distribution of Lead Accumulation (Soil, Water and Mangrove Vegetation) to Conserve Segara Anakan Lagoon Endang Hilmi; Asrul Sahri Siregar; Agung Dhamar Syakti
Journal Omni-Akuatika Vol 13, No 2 (2017): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (880.688 KB) | DOI: 10.20884/1.oa.2017.13.2.83

Abstract

Segara Anakan Lagoon  is a  specific lagoon as dispose industry waste area, i.e. lead waste.  Distribution of lead accumulation in vegetation, soil and water can explain pollution status in Segara Anakan Lagoon.  This study aims to analysis lead accumulation distribution  (soil, water and vegetation),  bioaccumulation factor (BAF), translocation factor (TF), leaf morphometric (the lead effect for mangrove vegetation), and to develop pre-design a mangrove  zoning based on lead accumulation. The results showed that lead accumulation in Segara Anakan Lagoon (SAL) was 0.177 – 0.233 mg/l (water),  0.320 – 0.780 mg/kg (soil),  4.80–8.67 mg/kg (mangrove roots), for stem between 2.48–6.96 mg/kg (mangrove stem), and 1.48–4.76 mg/kg (mangrove leaf).  BAF of mangrove vegetation scored between 22.2–40.1 and TF between 0.9–1.3. The lead accumulation of soil depths were 8.89 mg/kg (0-50 cm) to 0.56 mg/kg (150-200 cm). The effect of  lead accumulation was observed as leaf damage between 2-60 % of surface leafs. And based on best lead accumulation of mangrove vegetation showed that the first mangrove zoning was Rhizophora apiculata, Bruguiera sexangula, Aegiceras corniculatum and  Sonneratia caseolarisKeyword: lead accumulation; mangrove capacity; bioaccumulation factor; translocation factor;  lead effect
The Pattern of Herbivorous Fish Assemblages in The In Western and Eastern Outermost Island Indonesia Risandi Dwirama Putra; Rikoh Manogar Siringoringo; Muhammad Abrar; Ni Wayan Purnamasari; Agung Dhamar Syakti
Journal Omni-Akuatika Vol 16, No 2 (2020): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2020.16.2.805

Abstract

The herbivorous fishes have been considered as a critical functional group and have capability maintaining coral reef resilience and avoiding coral-algal phase-shifts. The present condition shown, almost in tropical reef location, alga has dominated coral, even in the small outer island. The requirement to conduct comprehensive basic research in studying the patterns and composition of herbivorous fish, especially on the small outer islands. Twelve coral reef sites in eastern Indonesia (Liki Islands) and western Indonesia (Natuna Island) used as a research location for comparing the structure patterns of herbivorous fish communities (diversity, density, and body size) using the Underwater Visual Census (UVC) method. There was different pattern of herbivorous fishes families in Liki Island and Natuna Islands, where Acanthuridae is dominant in eastern Indonesia (Liki Islands), including Ctenochaetus striatus (41,00 ± 11,72 se) individuals/350m2, A. maculiceps (23,33 ± 13,61 se) individuals/350m2, Naso hexacanthus (18,67 ± 6,34 se) individuals/350m2 while Scaridae is dominant in western Indonesia (Natuna island), including Scarus rivulatus (31,67 ± 10,61 se) individuals/350m2, Chlorurus sordidus (30,00 ± 8,52 se) individuals/350m2 and Scarus quoyi (19,00 ± 9,73 se) individuals/350m2. Based on herbivore fishes composition Liki Island has a higher density and biomass compared to Natuna Island.Keywords: herbivore, fish, coral, small outer island, Indonesia
Front-Matter for 13 (2): 1-198 Agung Dhamar Syakti
Journal Omni-Akuatika Vol 13, No 2 (2017): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

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Abstract

Marine Bioremediation in Indonesia : Die Before Blossom Agung Dhamar Syakti
Journal Omni-Akuatika Vol 14, No 3 (2018): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.097 KB) | DOI: 10.20884/1.oa.2018.14.3.584

Abstract

This critical review highlighted the need to booster an application of the environment biotechnology in order to reduce petroleum hydrocarbons contamination in marine environment.  Marine bioremediation can be promisingly applied when the spills occurred. However, absence of policy support from Indonesian government to endorse the private actor’s liabilities when complying the rehabilitation action was no significance while academic research was still far from establishment of fundamental knowledge in marine bioremediation. Thus, if so, it very likely that bioremediation disciplines and application might “die before blossom” in a country that often confronted by the maritime risk contamination of petroleum hydrocarbons on their marine and coastal area.