Article Per Year (5 Year)
p-Index From 2021 - 2026
2.48
P-Index
This Author published in this journals
All Journal Indonesian Journal of Pharmaceutical Science and Technology Pharmacon Journal La Lifesci Usadha Journal of Pharmacy
Cita Hanif Muflihah
Fakultas Farmasi, Universitas Muhammadiyah Surakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Published : 19 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : Pharmacon

 1 
Cytotoxic Assay of Semipolar Fraction Of Ethanolic Extract From Sugar Apple (Annona Squamosa L.) Stem Bark on T47D Cells Muflihah, Cita Hanif; Haryoto, Haryoto; Indrayudha, Peni
Pharmacon: Jurnal Farmasi Indonesia Vol 17, No 2 (2020)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v17i2.12268

Abstract

Previous research has shown that some compounds in leaves and seeds of sugar apple have a cytotoxic activity. The aim of this research was to determine the cytotoxicity of semipolar fraction of ethanolic extract from sugar apple stem bark (Annona squamosa L.) on T47D cancer cells. The semipolar fraction of ethanolic extract from sugar apple stem bark was collected by fractionation using Vacuum Liquid Chromatography (VLC) with hexane:ethyl acetic (9:1, 8:2, 7:3, and  6:4)  as  mobile  phase.  Cytotoxicity from the fractions of five different concentration namely; 25, 50, 100, 150, and 250, µg/mL was measured by MTT assay. The potency of the cytotoxicity was defined by the ability of the fraction to inhibit the growth of T47D cells indicated by the value of IC50. Qualitative analysis of contained compounds in the fraction was done by Thin Layer Chromatography (TLC) method using silica gel F 254 as a stationary phase and hexane:ethyl acetic (7:3) as a mobile phase. UV 254 and 366 nm lamp also Dragendorff, citroboric, and FeCl3 spray reagents were used to visualize the spots of the secondary metabolites. The result proved that the semipolar fraction of ethanolic extract from sugar apple stem bark showed potential cytotoxicity on T47D cancer cells with IC50value of 70,77 µg/mL. Qualitative analysis showed that the fraction contained flavonoids and alkaloids which is presumably responsible for its cytotoxic activity. 
Cytotoxic Assay of Semipolar Fraction Of Ethanolic Extract From Sugar Apple (Annona Squamosa L.) Stem Bark on T47D Cells Cita Hanif Muflihah; Haryoto Haryoto; Peni Indrayudha
Pharmacon: Jurnal Farmasi Indonesia Vol 17, No 2 (2020)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v17i2.12268

Abstract

Previous research has shown that some compounds in leaves and seeds of sugar apple have a cytotoxic activity. The aim of this research was to determine the cytotoxicity of semipolar fraction of ethanolic extract from sugar apple stem bark (Annona squamosa L.) on T47D cancer cells. The semipolar fraction of ethanolic extract from sugar apple stem bark was collected by fractionation using Vacuum Liquid Chromatography (VLC) with hexane:ethyl acetic (9:1, 8:2, 7:3, and  6:4)  as  mobile  phase.  Cytotoxicity from the fractions of five different concentration namely; 25, 50, 100, 150, and 250, µg/mL was measured by MTT assay. The potency of the cytotoxicity was defined by the ability of the fraction to inhibit the growth of T47D cells indicated by the value of IC50. Qualitative analysis of contained compounds in the fraction was done by Thin Layer Chromatography (TLC) method using silica gel F 254 as a stationary phase and hexane:ethyl acetic (7:3) as a mobile phase. UV 254 and 366 nm lamp also Dragendorff, citroboric, and FeCl3 spray reagents were used to visualize the spots of the secondary metabolites. The result proved that the semipolar fraction of ethanolic extract from sugar apple stem bark showed potential cytotoxicity on T47D cancer cells with IC50value of 70,77 µg/mL. Qualitative analysis showed that the fraction contained flavonoids and alkaloids which is presumably responsible for its cytotoxic activity. 
Combination of Ketepeng Cina (Cassia alata Linn) And Lidah Buaya Buaya (Aloe vera) As Antibacterial Agent Against Staphylococcus aureus And Pseudomonas aeruginosa Cita Hanif Muflihah; Az Zahra Miftahul Husna; Aminah Fitriana Hamka
Pharmacon: Jurnal Farmasi Indonesia Vol 20, No 1 (2023)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v20i1.22304

Abstract

Infection is one of the biggest health problems in Indonesia. Infection can be caused by pathogenic microorganisms such as Staphylococcus aureus and Pseudomonas aeruginosa. Treatment with antibiotics is the primary choice to manage infections caused by bacteria. Previous research showed that many plants have been tested for their antibacterial activity such as ketepeng cina (Cassia alata) and lidah buaya (Aloe vera). Cassia alata and Aloe vera alkaloids, flavonoids, anthraquinones, saponins, tannins contain similar active compounds as antimicrobials. Based on this, the purpose of this study was to determine the antibacterial activity of the combination of methanol extracts of C. alata and A. vera against S. aureus and P. aeruginosa bacteria. The antibacterial activity test was carried out using the Kirby-Bauer method. The single extract of C. alata and A. vera had the greatest antibacterial activity against S. aureus at a concentration of 10 mg/disk with zones of inhibition of 29.5±0.7 mm and 21.5±1.4 mm, respectively. The single antibacterial activity test of both extracts against P. aeruginosa bacteria showed no inhibition zone. The test results showed that the combination of 10 mg/disk C. alata methanol extract and 10 mg/disk Aloe vera could inhibit the growth of S. aureus with an average inhibition zone of 30.75±1.7 mm. However, the lower ratio of both extracts resulted in a likely antagonistic effect against S. aureus. Phytochemical screening tests showed that these two extracts had the same compounds, namely phenolics, flavonoids, anthraquinones. Contact bioautography showed that mainly phenolics, flavonoids, and anthraquinones were responsible in the antibacterial properties.
Immunomodulatory Activity of Kasumba Turate (Carthamus tinctorius L.) Extract Against Non-Specific and Specific Immune Responses in Mice Arini Fadhilah; Anisa Dwi Parwati; Cita Hanif Muflihah; Arifah Sri Wahyuni
Pharmacon: Jurnal Farmasi Indonesia Vol 20, No 1 (2023)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v20i1.22629

Abstract

Immunomodulators are compounds that can regulate the immune system with the objective of normalizing or helping to optimize the immune system. People tend to back to nature by utilizing various plants to be used as medicine, one of which is kasumba turate (Carthamus tinctorius L.) which is thought to be efficacious as an immunomodulator in increasing the activity and capacity of macrophages. This study aims to determine the immunomodulatory activity of kasumba turate ethanol (CTEE) extract against non-specific and specific immune responses in mice. Non-specific immune response tests using the carbon clearance method were carried out on mice with 3 dose ratings. Specific immune response tests were carried out by the hemagglutination test method to determine antibody titers. The results of the carbon clearance test showed that the CTEE dose 50 mg/KgBW was a moderate immunostimulant with a phagocytic index of 1.41, while doses of 100 and 200 mg/KgBW were strong immunostimulants with phagocytic index of 1.57 and 1.82 respectively. While the results of the hemagglutination test method, the antibody titers value is determined from the highest serum dilution value that still indicates hemagglutination. The results show an increase in humoral immune response with the highest primary and secondary antibody titer values of 1:256 and 1:1024 for doses of 100 mg/KgBW, and 1:512 and 1:1024 for doses of 200 mg/KgBW. Phytochemical screening results of CTEE proved to contain alkaloid group compounds, flavonoids, saponins, tannins and triterpenoids. Based on the results obtained, it was concluded that the administration of CTEE showed immunostimulant activity.
Co-Authors Aminah Fitriana Hamka Aminah Fitriana Hamka Anisa Dwi Parwati Anita Puji Maharani Arifah Sri Wahyuni Arini Fadhilah Astika, Fera Az Zahra Miftahul Husna Bakhtiar, Muhammad Fadhilah, Arini Fera Astika Hamka, Aminah Fitriana Haryoto Haryoto Indah Tri Puspita Maharani, Anita Puji Nindi Desinta Laila Putri Ningrum, Nur Faizah Setya Oksaputra, Amar Khoiri Peni Indrayudha Prastika, Rizky Wanda Pratiwi, Sekar Suci Puspaningrum, Devi Puspita, Indah Tri Putri, Nindi Desinta Laila Sikanaa, Arinaa Manaa