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All Journal Jurnal Biologi Udayana Bulletin of Electrical Engineering and Informatics Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease Journal of Agromedicine and Medical Sciences Journal of Public Health for Tropical and Coastal Region MIND (Multimedia Artificial Intelligent Networking Database) Journal NERSMID: Jurnal Keperwatan dan Kebidanan Biomolecular and Health Science Journal
Rohmah, Etik Ainun
Faculty Of Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Electrical & Electronics Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

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Mobile sensing in Aedes aegypti larva detection with biological feature extraction Dia Bitari Mei Yuana; Wahjoe Tjatur Sesulihatien; Achmad Basuki; Tri Harsono; Akhmad Alimudin; Etik Ainun Rohmah
Bulletin of Electrical Engineering and Informatics Vol 9, No 4: August 2020
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656.868 KB) | DOI: 10.11591/eei.v9i4.1993

Abstract

According to WHO, Dengue fever is the most critical and most rapidly mosquito-borne disease in the world over 50 years. Currently, the presence and detection of Aedes aegypti larvae (dengue-mosquitoes vector’s) are only quantified by human perception. In large-scale data, we need to automate the process of larvae detection and classification as much as possible. This paper introduces the new method to automate Aedes larvae. We use Culex larva for comparison. This method consists of data acquisition of recorded motion video, spatial movement patterns, and image statistical classification. The results show a significant difference between the biological movements of Aedes aegypti and Culex under the same environmental conditions. In 50 videos consisting of 25 Aedes larvae videos and 25 Culex larvae videos, the accuracy was 84%.
Kerentanan larva Aedes aegypti di Jawa Timur terhadap Temephos komersial dengan dosis berbeda Firas Khaleyla; Etik Ainun Rohmah; Kris Cahyo Mulyatno
Jurnal Biologi Udayana Vol 25 No 2 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i02.p08

Abstract

Control of Ae. Aegypti population is performed as an effort to suppress the transmission of dengue virus, one of the methods is sprinkling of temephos larvicide. Operational dose of temephos used in Indonesia (1 mg/L) is higher from recommended dose of WHO (0.012 mg/L). In East Java, commercial temephos are found to have different application doses of 8 g/L (8G) and 10 g/L (10 G). This study was designed to know the susceptibility of Ae. aegypti population from Jember (JEM), Surabaya (SBY), and Entomology Laboratory of Universitas Airlangga collection (LAB) towards temephos 8G and 10G starting at high (1 mg/L) to low concentrations(0.015625 mg/L). Larvae mortality test was performed based on WHO standard method with 4 times replication. Data was analyzed statistically. Results showed that the three groups of Ae. aegypti larvae had mortality level at >90% in the lowest concentration applied (0.015625 mg/L) at time threshold of 240 minutes, for both temephos8 G and 10 G. Significant difference of larvae mortality towards highest (1 mg/L) and lowest dose (0.015625 mg/L) was found in JEM, at both 8 G and 10 G doses, and LAB at 8 G dose. JEM and SBY groups were found to have resistance ratio 95 (RR95) to temephosat low category (<5) compared to LAB. All groupstill retained susceptibility towards commercial temephos larvicide at low dose (0.015625 mg/L). Based on results, we recommendedlowering the operational dose of larvicide temephos used for population control of Ae. aegypti vector population in East Java.
Breeding Preference and Bionomics of Anopheles spp. at the Malarial Endemic Area, Runut Village, East Nusa Tenggara Province, Indonesia Gery Morsales Munthe; David Nugraha; Gabriel Pedro Mudjianto; Etik Ainun Rohmah; Arnoldina Dolfina Dua Weni; Zukhaila Salma; Lynda Rossyanti; Fitriah; Suhintam Pusarawati; Budi Utomo; ukmawati Basuki; Haruki Uemura
Biomolecular and Health Science Journal Vol. 5 No. 1 (2022): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v5i1.35278

Abstract

Introduction: Anopheles mosquito is transmitting malaria, one of the health problems in Indonesia. Understanding Anopheles mosquito behaviour and its breeding preference is one of the crucial keys to prevent malaria transmission. This study aimed to identify the breeding place distribution and bionomics of Anopheles spp. in Runut village, Sikka district, East Nusa Tenggara. Methods: A descriptive observational study was conducted in Runut village, Waigete sub- district, in April 2018. Anopheles spp. larvae were collected in 7 suspected breeding places consisting of 2 rice fields, 3 fish ponds, and 2 puddles. Mosquitos behaviour was observed using bed-net traps located inside and outside the house from 7.15 PM to 1.15 AM after obtaining informed consent. Mosquito collection using bed-net trap were performed for 40 minutes then followed by resting mosquito collection for 10 minutes. Results: Anopheles spp. larvae were found in most of the suspected aquatic habitats, presenting different densities and together with larvae of the other mosquito species. Relatively high number of Anopheles spp. larvae was obtained from a puddle. Only one female mosquito of Anopheles spp. resting on the wall inside house was found around 00.55 – 01.05 AM and resulted in low mosquito density determination. Conclusion: Anopheles spp. larvae were harbouring in most of the aquatic habitats and one puddle contained moderately abundant larvae of Anopheles in Runut village, Sikka district, East Nusa Tenggara, Indonesia. Even only one Anopheles spp. mosquito was detected inside the house, residents in Runut village should regular use insecticide-treated bed nets and continuous observation of mosquito breeding places especially puddles to prevent malaria and other mosquito-borne diseases.
A Preliminary Study of Ocimum basilicum Essential Oil as a Repellent against Aedes aegypti in Surabaya Wahyuni, Afrinda Dwi; Zahra, Siti Fatimatuz; Putri, Balqis Qonita; Pramudya, Manikya; Rohmah, Etik Ainun; Mulyatno, Kris Cahyo
Journal of Public Health for Tropical and Coastal Region Vol 5, No 2 (2022): Journal of Public Health for Tropical and Coastal Region
Publisher : Faculty of Public Health, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jphtcr.v5i2.13734

Abstract

Introduction: Aedes aegypti is the main dengue vector in Indonesia. One of the dengue controls is through insecticides use. The plant-based insecticide is one alternative that can be used to minimize the impact of chemical insecticides. Ocimum basilicum is one of the plants that have the potency to be used as insecticides. The aims of this study were to analyze the insecticide activity of Ocimum basilicum essential oil against Aedes aegypti as well as its Lethal Concentration (LC50 and LC90) and Knockdown Time ( KT50 and KT90).Methods: Treatment groups were given Ocimum basilicum essential oil of 100 ppm, 50 ppm, and 25 ppm. The tests were performed four times with 10 mosquitoes per test.Results: The mortality rate of the mosquito on the extract with a concentration of 100 ppm was 100%, 50 ppm was 10% and 25 ppm was 0% and the value of LC50 = 62.9 ppm and LC90 = 78.7 ppm. KT50 of this study was 19.4 minutes and KT90 was 8.07 minutes.Conclusion: Ocimum basilicum essential oil has a mosquitocidal effect on Aedes aegypti. Further studies to investigate the substances of Ocimum basilicum against Aedes aegypti are needed.
The Longevity of Aedes aegypti Larvae in Several Water Sources in Surabaya Antonio Ayrton Widiastara; Gabriel Pedro Mudjianto; Etik Ainun Rohmah; Hengki Anggara Putra; Martha Indah Widia Ningtyas; Sri Wijayanti Sulistyawati; Suhintam Pusarawati; Fitriah; Kasiyama Desi Indriyani; Alpha Fardah Athiyyah; Basuki, Sukmawati
Indonesian Journal of Tropical and Infectious Disease Vol. 10 No. 1 (2022)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v10i1.32209

Abstract

Aedes aegypti transmits the dengue virus that causes Dengue Viring the high number of DVI cases is the existing breeding places of Ae. aegypti. The water sources used by the community and the surrounding environment are essential media for living Ae. aegypti larvae. This recent study aimed to detect the longevity of Ae. aegypti larvae in diff erent water sources in Surabaya and the killing effect of temephos. An analytical observational and experimental study was conducted in August-September 2021. Twenty-instar III Ae. aegypti larvae were put in each 100 ml beaker glass containing diff erent water sources, such as rain, well, mineral, new and used bath water, and antiseptic soapy water. Fungi in water sources were examined. Two groups were set with and without temephos, the final temephos concentration was of 0.00001 ppm. Live Ae. aegypti larvae, pupae, mosquitoes were observed every 24 hours for seven days without feeding. Living larvae were still found on Day 7 in all water sources with and without temephos. There were more larvae live in soapy water without temephos, particularly on Day 2 to Day 6, compared to other water sources either without or with temephos. In contrast, many larvae died in mineral water with temephos. Some larvae turned into pupae, started on Day 1. Pupae and mosquitoes were mostly found in rain water with temephos. Ae. aegypti larvae survived better in soapy water either with or without temephos. Temephos seemed to be effective to kill Ae. aegypti larvae in mineral water, and might induce larvae in turning to pupae and mosquitoes quickly at low concentration.
Skrining Fitokimia Polifenol Ekstrak Etanol Daun Ubi Jalar Kuning (Ipomea batatas) dari Madiun Terza Aflika Happy; Vivin Indrianita; Etik Ainun Rohmah
NERSMID : Jurnal Keperawatan dan Kebidanan Vol. 6 No. 1 (2023): Mei
Publisher :

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55173/nersmid.v6i1.151

Abstract

Yellow sweet potato (Ipomoea batatas L.) leaves are an easy source of polyphenols because they are often not used after harvest. This leaf began to be used as an herbal ingredient or alternative treatment for the community, empirically showing improvement in conditions and symptoms in Dengue Fever patients. This study determined the content of secondary metabolites from yellow sweet potato leaves. Methods: Research using laboratory testing methods was carried out at the Herbal Materia Medika Laboratory in Batu. Yellow-green sweet potato leaves, as much as 200 grams were collected from the Madiun, East Java. It was extracted by maceration using 2L of ethanol 96% for 2 days and evaporated in a vacuum rotary evaporator to thick extract. Furthermore, phytochemical screening tests were carried out. Results: The results of this study indicated that the yellow sweet potato leaves contained of Alkaloids, Flavonoids, Tannins, Saponins, Terpenoids, Triterpenoids, and Phenols. So the yellow sweet potato leaves are very representative as herbal products.
Toxicity of Ceremai (Phyllanthus Acidus) and Zodia (Evodia Suaveolens) Leaf Extracts on Mortality of Aedes Aegypti Larvae Imtiyaz, Andi Hilman; Armiyanti, Yunita; Ulfa, Evi Umayah; Rohmah, Etik Ainun
Journal of Agromedicine and Medical Sciences Vol 10 No 3 (2024)
Publisher : Faculty of Medicine, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v10i3.48674

Abstract

Aedes aegypti is the vector that carries Dengue Hemorrhagic Fever (DHF). Various strategies have been implemented to control the spread of the Aedes aegypti, one of which is using temephos larvicide. The use of temephos causes Aedes aegypti resistance and environmental pollution. Therefore, it is necessary to develop a bio-larvicide using ceremai leaf (Phyllanthus acidus) and zodia leaf (Evodia suaveolens). This study aimed to determine the toxicity of ceremai leaf and zodia leaf extracts on the mortality of Aedes aegypti larvae. This type of research is a pure experiment using a Completely Randomized Design. The research sample was third instar Aedes aegypti larvae. There were 7 groups consisting of 1 negative control (aquadest and tween20), 1 positive control (temephos 0.008%) and 5 treatment groups (ceremai leaf extract and zodia leaf extract). Larval mortality data were observed after 24 hours of exposure. The results of the Kruskal Wallis test showed that there was a difference in mortality between groups with a value of p : 0.020 (p < 0.05). Zodia leaf extract at 0.2% has the highest toxicity to the mortality of Aedes aegypti larvae. The probit test results of zodia leaf extract showed LC50 and LC90 values of 0.075% and 0.121% within 24 hours of exposure. Zodia leaf ethanol extract has effectiveness as larvicide of Aedes aegypti. Keywords: Aedes aegypti, larvicide, extract, ceremai, zodia
ISOLATION OF Streptomyces sp. FROM LAPINDO MUD SOIL, SIDOARJO, EAST JAVA PROVINCE, INDONESIA AS A LARVICIDE CANDIDATE AGAINST Aedes aegypti Yotopranoto, Subagyo; Kurnijasanti, Rochmah; Rohmah, Etik Ainun
Folia Medica Indonesiana Vol. 53 No. 2 (2017): JUNE 2017
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.549 KB) | DOI: 10.20473/fmi.v53i2.6355

Abstract

Streptomyces sp. is a bacterium that can live in many kind of habitations e.i. marine, sea sponge, coastal area, soil, desert soil, river bank etc. Many antibiotics and secondary metabolites are produced by this bacterium. Several secondary metabolites of the bacterium can be used as an insecticide against insects including mosquito larva.The purpose of this study was to search and isolate until species of Streptomyces sp. from Sidoarjo Lapindo mud soil in East Java province that can be used as a larvicide against Aedes aegypti larva, the dengue haemorrhagic fever vector.The method of study was to collect several mud soil samples from Sidoarjo Lapindo. The collected samples were cultured in ISP-4 media for producing mix-cultures. Then, to isolate the suspected colony of Streptomyces sp. by culturing again in several replications on ISP-4 media in petri-dish. The pure isolates were cultured in ISP-4 slant media. There were could be obtained eight pure isolates of Streptomyces sp.The characterization of 16S rRNA of Streptomyces sp. was done in order to determine the species. DNA isolation was done and followed by DNA sequencing, then compared to Gene Bank with BLAST program. The results showed that the sequence nucleotide bases of Streptomyces Sp-D6 had high similarity to Streptomyces sp. 171524, beside the sequence nucleotide bases of Streptomyces Sp-D7 and Sp-D9 had high similarity to Strepto-myces sp. ACT-01578 and ACT-175695. These three species can be used as larvicide candidate against Ae. aegypti.
Sarana Pelaporan Angka Bebas Jentik dan Deteksi Jentik Nyamuk menggunakan Deep Learning YUANA, DIA BITARI MEI; AGASTA, IRA AMELIA; SAPUTRO, MUHAMMAD ADI; ROHMAH, ETIK AINUN
MIND (Multimedia Artificial Intelligent Networking Database) Journal Vol 10, No 1 (2025): MIND Journal
Publisher : Institut Teknologi Nasional Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26760/mindjournal.v10i1.89-98

Abstract

AbstrakDemam Berdarah Dengue (DBD) masih menjadi masalah kesehatan utama di Indonesia. Kabupaten Jember mencatat 1.627 kasus pada tahun 2024, dengan Angka Bebas Jentik (ABJ) hanya mencapai rata-rata 92%, di bawah standar nasional >95%. Penelitian ini mengembangkan sistem deteksi jentik nyamuk otomatis menggunakan metode Deep Learning berbasis CNN dan GRU. Fitur visual diekstraksi melalui model InceptionV3, kemudian dianalisis secara sekuensial oleh GRU untuk klasifikasi larva. Hasil menunjukkan model mencapai akurasi pelatihan dan pengujian dengan performa optimal pada epoch ke-20 sebesar 99.19%, loss 0.0419. Jika dibandingkan dengan metode sebelumnya (AOA) yang hanya mencapai 84%, pendekatan ini terbukti lebih akurat dan tahan terhadap variasi kondisi data.Kata kunci: Demam Berdarah Dengue, Aedes aegypti, Angka Bebas Jentik, Deep Learning, Gated Recurrent Unit, Deteksi OtomatisAbstractDengue Hemorrhagic Fever (DHF) remains a major public health issue in Indonesia. In 2024, Jember Regency recorded 1,627 cases, with the Larvae Free Index (LFI) averaging only 92%, below the national standard of >95%. This study developed an automatic mosquito larvae detection system using a Deep Learning approach based on CNN and GRU. Visual features were extracted using the InceptionV3 model and then analyzed sequentially by the GRU for larval classification. The results showed that the model achieved optimal training and testing performance at the 20th epoch with 99.19% accuracy and a loss of 0.0419. Compared to the previous method AOA, which achieved only 84% accuracy, this approach proved to be more accurate and robust against variations in data conditions.Keywords: Dengue Hemorrhagic Fever, Aedes aegypti, Larvae-Free Rate, Deep Learning, Gated Recurrent Unit, Automated Detection
Co-Authors Achmad Basuki AGASTA, IRA AMELIA Alimudin, Akhmad Alpha Fardah Athiyyah Antonio Ayrton Widiastara Arnoldina Dolfina Dua Weni BUDI UTOMO David Nugraha Dia Bitari Mei Yuana Evi Umayah Ulfa Fitriah Gabriel Pedro Mudjianto Gery Morsales Munthe Haruki Uemura Hengki Anggara Putra Imtiyaz, Andi Hilman Kasiyama Desi Indriyani Khaleyla, Firas Kris Cahyo Mulyatno Kris Cahyo Mulyatno, Kris Cahyo Manikya Pramudya Martha Indah Widia Ningtyas Muhammad Adi Saputro Putri, Balqis Qonita Rochmah Kurnijasanti Rossyanti, Lynda Sriwijayanti Sulistyawati Subagyo Yotopranoto Suhintam Pusarawati Sukmawati Basuki Terza Aflika Happy Tri Harsono ukmawati Basuki Vivin Indrianita Wahjoe Tjatur Sesulihatien Wahyuni, Afrinda Dwi Yuana, Dia Bitari Mei Yunita Armiyanti Zahra, Siti Fatimatuz Zukhaila Salma