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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Agrikultura Bionatura Cropsaver : Journal of Plant Protection Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Perlindungan Tanaman Indonesia AGROLOGIA : Jurnal Ilmu Budidaya Tanaman Agrikultura Masyarakat Tani: Jurnal Pengabdian kepada masyarakat
Ceppy Nasahi
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health

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Resistance of Nilaparvata lugens to Fenobucarb and Imidacloprid and Susceptibility to Neem Oil Insecticides Danar Dono; Neneng S Widayani; Safri Ishmayana; Yusup Hidayat; Fitri Widiantini; Ceppy Nasahi
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.234-244

Abstract

This study aims to monitor and determine the level of resistance of N. lugens to fenobucarb and imidacloprid, to analyse of the specific activities of asetylcholinesterase, Glutation S-Transferase, esterase, and to determine sensitivity of resistant N. lugens to the neem oil. Resistance tests were conducted on a field population from Cipunagara, Subang, West Java, Indonesia, and a standard population originating from Banyuwangi, East Java, that was obtained from the Indonesian Center for Rice Research. Synthetic and neem Insecticides were applied using the leaf-stem dipping method. The resistance ratio obtained by comparing the LC50 value of the field population with the standard. The test insects were considered to be resistant to synthetic insecticide if they had a resistance ratio (RR) of more than 4, indicated resistance if RR ≥1, and susceptible to neem if has RR <1. The results of the research showed that the N. lugens population of Cipunagara indicated resistance to fenobucarb (RR = 2.43), while it was clearly resistance to imidacloprid (RR = 13.95). Specific activities of the acetylcholinesterase and Glutathione S-Transferase of the N. lugens Cipunagara populations were higher than the standard, while the esterase specific activity was lower. These two enzymes are responsible for the resistance of N. lugens to fenobucarb and imidacloprid. Nevertheless, the N. lugens population of Cipunagara was susceptible to neem (RR <1) that never been reported before. Therefore, it is important to know the change in the sensitivity of the target and the metabolic pathway of the insecticide used to manage insect resistance.
Potensi Metabolit Sekunder Asal Bakteri Endofit dalam Menekan Pertumbuhan Miselium Ganoderma boninense Fitri Widiantini; Ceppy Nasahi; Endah Yulia; Serly Noviyawati
Jurnal Fitopatologi Indonesia Vol. 14 No. 3 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (598.628 KB) | DOI: 10.14692/jfi.14.3.104

Abstract

Potency of Endophytic Bacterial Secondary Metabolite to Inhibit Mycelium Growth of Ganoderma boninense Endophytic bacteria have a close relationship with their host plants. The bacteria are living inside their host without causing any symptom. Endophytic bacteria are well known for their ability to produce compounds that can be used to protect plants from deleterious microorganisms. The research was conducted to determine the ability of endophytic bacteria in producing antifungal compounds that are able to suppress the growth of Ganoderma boninense, a causal agent of basal stem rot disease of oil palm. Previously isolated endophytic bacteria were grown on international streptomyces project 2 (ISP2) liquid medium and incubated at rotary evaporator at 120 rpm for 10 days. The supernatant was separated from bacterial cells and tested against G. boninense using well diffusion agar method.  The results showed that endophytic bacteria isolate of BEK6, BEK7, BEK9 and BEK10 were able to produce antifungal compound against G. boninense. Microscopic observation demonstrated G. boninense mycelia alteration following secondary metabolite application. 
Density, Distribution and Population Structure of Apple Golden Snail (Pomacea canaliculata L.) in Organic and Conventional Paddy field Ecosystems Vira Kusuma Dewi; Brinadia Solihati; Wawan kurniawan; Ceppy Nasahi; Nur Fitrianti
CROPSAVER Vol 4, No 2 (2021)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v4i2.37560

Abstract

Golden snail is one of the important pests that attack paddy crops. The structure of golden snail is divided into 3 based on the size of the shell diameter, namely juveniles (0.5-1 cm), preadults (1-2.5 cm), and adults (2.5-4 cm). The paddy cultivation system can affect the golden snail population. The purpose of this study was to determine the density, distribution and population structure of the golden snail (Pomacea canaliculata L.) with case studies in Cicalengka (conventional field) and Ciparay (organic field), District of Bandung, from October 2020-January 2021. This research used survey method with sampling technique in purposive sampling.  At each location divided into 5 fields with sized 10x5 m which were divided into 7 quadrant plots. Sampling was carried out at the age of paddy crops 14,21,28, and 35 days after planting. The observerd parameters were density, distribution pattern using the Index of Morisita (Id) and population structure of P. canaliculata. The results showed that the density of P. canaliculata in organic field was higher than in conventional field. The average population structure of pre-adult golden snails in organic and conventional rice fields was significantly different at 14-35 days after planting (DAP) and adult golden snails at 14, 28, and 35 DAP. Distribution pattern of P. canaliculata in organic and conventional were grouped.
The Effectiveness of Neem Cake (Azadirachta indica) Water Extract against Aphids (Aphis gossypii) on Red Chili Plant Lathifah Azizah; Ceppy Nasahi; Danar Dono
CROPSAVER Vol 4, No 2 (2021)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v4i2.36634

Abstract

Aphis gossypii is one of the factors that reduce chili production which acts as a pest and virus vector. This research aimed to determine the effectiveness of the application of neem cake water extract to A. gossypii on red chili plants. This research consisted of 2 experimental stages, namely in the laboratory and the greenhouse trials. The experimental design in the laboratory used a completely randomized design of neem cake water extract with treatments is A (Control), B (0.3%), C (0.6%), D (1.2%), E (2.4%), F (4.8%). Each treatment replicate 4 times. Aplication of water neem cake extract by dipping method of chilli leaf. After the leaf feed air dried, then the leaf put into plastic box and invested with 10 nymph of A. gossypii. While the experiments in the greenhouse used a randomized block design of neem cake water extract treatments were A (Control), B (LC50), C (1.5xLC50), D (2xLC50), E (2.5xLC50), F (3xLC50). The concentration of neem extract in the greenhouse experiment based on result of experiment at laboratory. Adults of A. gossypii as much as 20 were invested on every red chili plant. Application of neem cake water extract on red chili plants once every 4 days. The results showed that neem cake water extract effective to  A. Gossypii with 0.14% as LC50. Neem cake water extract at concentration of 1.5xLC50 (0.21%) was effective suppressing A. gossypii population on red chili plants.
Effect of Betel Leaf (Piper sp.) Water Extracts to Control Penicillium digitatum Causes of Green Mold in Dekopon Citrus (Citrus reticulata) Ceppy Nasahi; Ria Aghaselly Clonelin
CROPSAVER Vol 4, No 1 (2021)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v4i1.33913

Abstract

Green mold disease (Penicillium digitatum Sacc.) is one of the main problems in citrus fruits. Some studies had made use of biopesticides in treating postharvest diseases. Some studies reported that plant extracts can suppress various pathogens caused to have antifungal characteristics. One of the plants that are widely used as an extract is green betel leaf (Piper betle) and red betel (Piper ornatum).. This study aims to determine the effect of water extracts from green betel leaf  and red betel leaf in suppressing P. digitatum in dekopon citrus (Citrus reticulata Shiranui). The experiment used the experimental method of Completely Randomized Design with 7 treatments and 3 replications. The results showed that the water extracts of green betel leaf and red betel can inhibit the pathogen P. digitatum both in vitro and in vivo. The red betel leaf water extract at concentration of 10% was able to inhibit the colony diameter of P. digitatum in vitro at 43.61% and green betel lef extract at concentration of 10%  was 39.34%. The best inhibition of green water extract and red betel the diameter of green mold disease on dekopon fruit was 15.95% and 39.74%, respectively. The water extracts of green betel leaf and the red betel leaf were able to inhibit green mold disease in vivo successively was 0.52-19.62% and 17.64-52.67%. The treatment of 10% green betel water extract was able to suppress the growth of green mold disease in dekopon fruit was 19.62% and 10% red betel water extract inhibition was 19.62%.
Effect of the Initial Temperature of Extraction of Neem Cake (Azadirachta indica A. JUSS) on its Toxicity on Crocidolomia pavonana (F.) Larvae Fhera Hardiani; Danar Dono; Ceppy Nasahi
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.197 KB) | DOI: 10.24198/cropsaver.v2i1.22704

Abstract

Crocidolomia pavonana is an important pest that attacks the Brassicaceae. Neem cake is waste from neem seed pressing that can be used as a botanicals insecticide to control insect pests. The effect of the initial temperature of extraction can have an impact on the toxicity of the extract. The aim of this study was to determine the best initial temperature for the extraction of neem cake on mortality, larval development time, feed consumption, larval weight and survival of larvae into pupae and adult. The experimental method used in this research was Randomized Completely Design (RCD) with 4 treatments and 4 replication, i.e. control, extraction of neem cake at initial temperature of 30°C, initial temperature 50°C, and initial temperature 70°C which allows two concentrations of 1% and 3%. The results of the research with the best toxicity were shown in the initial extraction temperature of 50°C at concentrations of  1% and 3% with the mortality reaching 82.5% and 90%. The initial temperature extraction of 50°C also showed lengthened of the larval development time, decreased feeding activity, larval weight and survival of larvae to develop into pupae and imago.
Penambahan Gula pada Medium Biakan untuk Meningkatkan Pertumbuhan dan Kemampuan Antagonisme Candida tropicalis terhadap Patogen Penyebab Penyakit Layu Fusarium Tomat Sri Hartati; Timotius A. Sumbari; Ceppy Nasahi; Wawan Kurniawan
Agrikultura Vol 31, No 2 (2020): Agustus, 2020
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v31i2.28544

Abstract

Layu fusarium merupakan salah satu penyakit utama pada tanaman tomat yang disebabkan oleh Fusarium oxysporum f.sp. lycopersici (FOL) Salah satu agens antagonis yang dapat menghambat pertumbuhan FOL adalah khamir Candida tropicalis. Pertumbuhan dan kemampuan antagonisme khamir dapat ditingkatkan dengan penambahan gula sebagai sumber nutrisi. Penelitian ini bertujuan untuk mendapatkan konsentrasi dan jenis gula yang dapat meningkatkan pertumbuhan dan antagonisme C. tropicalis terhadap FOL. Percobaan dilakukan dengan menggunakan Rancangan Acak Lengkap yang terdiri atas 16 perlakuan dan 3 ulangan. Perlakuan terdiri atas jenis gula yang ditambahkan pada medium tumbuh khamir yaitu glukosa, sukrosa, trehalosa, D-xylose, dan D-mannitol dengan konsentrasi masing-masing gula 1%, 2,5%, 5%, dan kontrol (PDA setengah dekstros dan PDA dekstros penuh). Hasil percobaan menunjukkan bahwa D-mannitol 1% mampu meningkatkan panjang koloni C. tropicalis tertinggi sebesar 4,80 cm, dibanding kontrol (4,07 cm). Sukrosa 2,5% mampu meningkatkan lebar koloni tertinggi sebesar 1,47 cm, dibanding kontrol (1,00 cm). Seluruh perlakuan mampu meningkatkan kerapatan sel khamir C. tropicalis.  Kerapatan sel tertinggi dihasilkan oleh trehalosa 1% sebesar 4,27 x 107, sementara pada kontrol 0,1 x 107 sel/ml. Gula trehalosa 2,5% dapat meningkatkan antagonisme khamir dengan daya hambat tertinggi sebesar 23,68%, dibandingkan dengan kontrol PDA dekstros penuh.
Penambahan Essens Buah terhadap Keefektifan Metil Eugenol dalam Menarik Bactrocera spp. Drew & Hancock Agus Susanto; Ceppy Nasahi; Yuri Khansa Rumaisha; Wayan Murdita; Tri Murniningtyas Puji Lestari
Agrikultura Vol 30, No 2 (2019): Agustus, 2019
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (637.369 KB) | DOI: 10.24198/agrikultura.v30i2.23315

Abstract

Lalat buah (Bactrocera carambolae) merupakan hama yang sangat merugikan pada komoditas hortikultura. Penelitian ini bertujuan untuk mendapatkan jenis essens buah yang ditambahkan pada metil eugenol yang tertinggi dalam menarik lalat buah, untuk mengetahui pengaruh penambahan essens jambu pada metil eugenol terhadap jumlah tangkapan lalat buah pada pertanaman jambu biji, dan berapa konsentrasi essens yang menghasilkan jumlah tangkapan lalat buah paling banyak. Penelitian secara in vitro dilakukan di Laboratorium Vapor Heat Treatment, Balai Peramalan Organisme Pengganggu Tumbuhan, Jatisari, Jawa Barat dari bulan Februari 2015 hingga Juni 2015 dan di kebun jambu biji Desa Leuwiseeng, Kecamatan Panyingkiran, Kabupaten Majalengka sejak bulan November 2015 sampai dengan Maret 2016. Hasil penelitian menunjukan bahwa metil eugenol yang ditambah essens belimbing dan jeruk berpengaruh dalam meningkatkan hasil tangkapan lalat buah betina, tetapi tidak untuk lalat buah jantan. Perlakuan tertinggi dalam menarik lalat buah betina yaitu sebesar 37% dari total populasi ditemukan pada perlakuan penambahan essens belimbing. Sementara perlakuan 0,2 ml metil eugenol menarik sebanyak 10% dari total populasi. Pencampuran metil eugenol dan esens buah jambu mampu menarik lalat buah jantan lebih tinggi dibandingkan penggunaan tunggal metil eugenol dan penggunaan tunggal esens jambu. Pencampuran metil eugenol 0,2 ml + esens buah jambu 0,8 ml menunjukkan hasil jumlah tangkapan tertinggi lalat buah jantan sebanyak 15.145 ekor. Namun, pencampuran metil eugenol dan esens jambu tidak mempengaruhi jumlah tangkapan lalat buah betina. Spesies lalat buah yang paling dominan hasil identifikasi pada pertanaman jambu biji di Desa Leuwiseeng, Majalengka, adalah B. dorsalis 64% dan B. carambolae 36%.Kata kunci : Essens, Bactrocera carambolae, Metil eugenol
Potensi antagonisme senyawa metabolit sekunder asal bakteri endofit dengan pelarut metanol terhadap jamur G. boninense Pat. Fitri Widiantini; Endah Yulia; Ceppy Nasahi
Agrikultura Vol 29, No 1 (2018): April, 2018
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (641.661 KB) | DOI: 10.24198/agrikultura.v29i1.17870

Abstract

ABSTRACTAntagonism potency of secondary metabolites produced by endophytic bacteria in methanol against pathogenic fungi Ganoderma boninense Pat.The research aimed to determine the antifungal effect of secondary metabolites produced by endophytic bacteria of healthy root oil palm tree against the growth of Ganoderma boninense, the causal agent of basal stem rot disease on oil palm tree. Endophytic bacteria isolates (BEK5, BEK6, BEK7, BEK8, BEK9, BEK10 dan BEK11) were grown on ISP2 agar media for 14 days and extracted using methanol. Following extraction, the methanol was evaporated using rotary evaporator and the filtrat was sterilized using membrane filter 0.2 μm. The effect of the secondary metabolites against G. boninense was tested using agar well diffusion method. The observation on the colony growth and morphologicy of G. boninense mycelia were done at 7 days after treatment. The result demonstrated that all of the endophytic bacteria were able to produce seconday metablites that has antifungal effect on the growth of G. boninense. The highest growth inhibition was shown by secondary metabolites produced by BEK6 with inhibition of 22.89%. Furthermore, the secondary metabolites produced by all of the endophytic bacteria were caused morphological changes on the mycelia of G. boninense.Keywords; Antifungal, Inhibition, MalformationABSTRAKPenelitian ini bertujuan untuk mengetahui kemampuan antijamur metabolit sekunder yang dihasilkan oleh bakteri endofit asal akar tanaman kelapa sawit untuk menghambat pertumbuhan jamur patogen Ganoderma boninense, penyebab penyakit busuk pangkal batang pada tanaman kelapa sawit. Isolat-isolat bakteri endofit (BEK5, BEK6, BEK7, BEK8, BEK9, BEK10 dan BEK11) ditumbuhkan pada media ISP2 agar selama 14 hari dan kemudain diekstraksi dengan pelarut metanol. Metanol diuapkan menggunakan rotary evaporator dan filtrat yang dihasilkan disterilkan menggunakan membran filter berukuran 0,2 μm. Pengujian pengaruh senyawa metabolit sekunder terhadap pertumbuhan jamur G. boninense dilakukan dengan metode agar well diffusion. Pengamatan terhadap pertumbuhan koloni jamur G. boninense dan morfologi miselia G. boninense dilakukan pada 7 hari setelah perlakuan. Hasil penelitian menunjukkan semua isolat bakteri endofit mengeluarkan senyawa metabolit sekunder yang dapat menghambat pertumbuhan jamur G. boninense. Penghambatan pertumbuhan koloni jamur G. boninense tertinggi sebesar 22,89% ditemukan pada perlakuan metabolit sekunder asal bakteri BEK6. Pengamatan terhadap morfologi jamur G. boninense menunjukkan bahwa senyawa metabolit sekunder yang dihasilkan oleh isolat-isolat bakteri endofit tersebut dapat menyebabkan perubahan morfologi miselia G. boninense.Kata kunci: Antifungal, Penghambatan, Malformasi
Inventarisasi Penyakit pada Tanaman Jarak Pagar (Jatropha curcas L.) Pada Tiga Daerah di Jawa Barat Krisna Dwi Laksono; Ceppy Nasahi; Nenet Susniahti
Agrikultura Vol 21, No 1 (2010): April, 2010
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2341.589 KB) | DOI: 10.24198/agrikultura.v21i1.975

Abstract

Tanaman jarak pagar (Jatropha curcas L.) adalah salah satu tanaman alternatif penghasil minyak nabati yang berpotensi untuk dikembangkan di Indonesia. Oleh karena itu, faktor-faktor yang mempengaruhi pengembangan jarak pagar perlu diperhatikan termasuk penyakit tanaman. Penelitian ini bertujuan untuk menginventarisasi penyakit tanaman yang terdapat di beberapa perkebunan jarak pagar di daerah Jawa Barat yaitu, PT. Genting Oils di Cirata Kabupaten Bandung, PT. Rajawali Nusantara Indonesia (RNI) di Jatitujuh Kabupaten Majalengka, dan PT. RNI di  Purwadadi Kabupaten Subang. Metode yang digunakan dalam penelitian ini adalah metode survei dengan pengamatan keberadaan penyakit secara langsung pada tanaman sampel. Hasil pengamatan menunjukan bahwa penyakit embun tepung yang disebabkan oleh Oidium sp. dengan intensitas penyakit mencapai 38 %  ditemukan di  kebun Cirata B. Penyakit antraknosa yang disebabkan oleh colletotrichum sp. dengan intesitas penyakit mencapai 37 % ditemukan di kebun Cirata A. Penyakit embun jelaga yang disebabkan oleh Capnodium sp. terdapat di kebun Purwadadi dan Jatitujuh dengan intensitas penyakit mencapai masing-masing 36 % dan 22,5 %.  Selain itu, ditemukan juga penyakit layu bakteri yang disebabkan oleh Pseudomonas sp., penyakit hawar daun kelabu yang disebabkan oleh Pestalotiopsis sp., serta ditemukan juga keberadaan nematode Rotylenchulus sp. di seluruh area penelitian.
Co-Authors Abraham Suriadikusumah Agus Susanto Amatullah, Hana Lathifah Andang Purnama Anggi Ayuningtyas Brinadia Solihati Danar Dono Danar Dono Danar Dono Danar Dono Danar Dono Denny Kurniadie Dwisari Januarily Pitaloka Emelda Anggraini Endah Rismawati Endah Yulia Endah Yulia Endah Yulia Yulia Fhera Hardiani Fitri Widiantini Krisna Dwi Laksono Lathifah Azizah Luciana Djaya Masturina, Safirah Amalia Mochamad Arief Soleh, Mochamad Arief Nasya Nabila Natawisastra, Dang Aditya Neneng S Widayani Nenet Susniahti Noor Istifadah Nugraha, Gema Takbir Nur Fitrianti Rahmah, Syifa Aulia Ramadhan Kamal Putra Ria Aghaselly Clonelin RIKA MELIANSYAH Safri Ishmayana Serly Noviyawati Siska Rasiska Sri Hartati Sri Hartati Sri Hartati Subakti-Putri, Syifa Nabilah Sudarjat Sudarjat Susanto, Ainun Nandini Putri Syarif Hidayat SYARIFUL MUBAROK Tarkus Suganda Timotius A. Sumbari Toto Sunarto Tri Murniningtyas Puji Lestari Vira Kusuma Dewi Wawan Kurniawan Wayan Murdita Widayani, Neneng Sri Yulia, Endah Yulia Yuri Khansa Rumaisha Yusup Hidayat Yusup Hidayat