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All Journal Nurani: Jurnal Kajian Syariah dan Masyarakat Veterinary Biomedical and Clinical Journal Journal of Parasite Science
Kusnoto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Social Sciences Veterinary

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Journal : Veterinary Biomedical and Clinical Journal

 1 
The Identification of Hysterothlacium on Sixbar Groupers (Epinephelus sexfasciatus) in The Territorial Waters of East Java Using Sequencing Method: Identifikasi Hysterothylacium sp. pada Ikan Kerapu Belang (Epinephelus sexfasciatus) di Perairan Jawa Timur Menggunakan Metode Sekuensing Kismawardani, Putri; Koesdarto , Setiawan; Santoso , Kuncoro Puguh; Lastuti , Nunuk Dyah Retno; Sabdoningrum , Emy Koestanti; Kusnoto
Veterinary Biomedical and Clinical Journal Vol. 5 No. 1 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.01.2

Abstract

This study aimed to identify the parasitic nematode Hysterothylacium sp. using the sequencing method. A sampling of Hysterothylacium sp. was taken from 60 striped grouper fish (Ephinephelus sexfasciatus) and has characteristics such as brown color, booring teeth on the anterior, a simple digestive system including the esophagus, stomach (ventriculus), small intestine and does not have mucron on the posterior. Specific primers for the COX-2 mtDNA gene (210F and 211R) were used for DNA amplification (Polymerase Chain Reaction) and DNA sequencing with a length of 629bp. The positive result of the PCR method (sample S) was sequenced. Then, the results of the sequencing method were compared with the reference DNA sequences KY065298.1, MF120253.1, MF120248.1, and KU886687.1. Furthermore, Homology and kinship tests were carried out using GenBank (NCBI). Results showed a 97% similarity of the sample with Hysterothylacium amoyense cytochrome oxidase subunit II (COX-2) gene MF120253.1.
Parasites Prevalence of Dairy Cattle in Argopuro Area, Krucil District, Probolinggo Regency, Indonesia Pradana, Giffari Danindra; Mufasirin; Madyawati, Sri Pantja; Tri Suwanti, Lucia; Kusnoto; Sunarso, Agus; Aryaloka, Suhita
Veterinary Biomedical and Clinical Journal Vol. 5 No. 2 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.02.3

Abstract

This study aimed to identify endoparasitic species and their prevalence in dairy cows in the Probolinggo, Indonesia. This survey was conducted in the Cooperation of Argopuro, in the hill side of Krucil district, Probolinggo regency, during rainy season from March until July 2020. Faecal samples were collected (n=100), and three fecal examinations were performed for parasite identification: native, sedimentation, and flotation techniques. Results showed that the prevalence of endoparasitosis was 56%; 29% was due to helminthiases and the other 37% was of Balantidium coli. Fasciola sp., Oesophagostomum sp., Gaigeria pachyscelis, Toxocara vitulorum, Mecistocirrus digitatus, Chabertia sp. were among the helminths detected. The Lucient Brump test indicated that among samples infected with helminths (n=29), 89.7% were mild, 6.9% were moderate and 3.4% were severely infected. Further, the study estimated that the level of burden with Balantidium coli was identified to be mild in 62.2%, moderate in 32.4%, and severe in 5.4% of the positive samples, respectively (n=37). This study indicates that during the commencement of the rainy season, the campaign of effective endoparasitic control could be advisable in the study area
Co-Authors Alasrorik, Muhammad Hizbulloh Aryaloka, Suhita Athoillah, Mohamad Anton Bambang Waluyo Dian Ayu Permatasari Djoko Legowo DWI PUTRI RAHMAWATI Endang Suprihati Hani Plumeriastuti Heni Puspitasari Ihda Hanny, Khurun'In Fadia Imam Mustofa Kismawardani, Putri Koesdarto , Setiawan Lastuti , Nunuk Dyah Retno Lucia Tri Suwanti, Lucia Tri Mufasirin Niluh Selly Frantika Poedji Hastutiek Pradana, Giffari Danindra Sabdoningrum , Emy Koestanti Santoso , Kuncoro Puguh Sholehudin, Ending Sri Pantja Madyawati, Sri Pantja Sunarso, Agus Tri Suwanti, Lucia Uu Nurul Huda, Uu Nurul