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All Journal EKSAKTA: Journal of Sciences and Data Analysis JURNAL KIMIA SAINS DAN APLIKASI Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Jurnal Kimia dan Kemasan EduChemia: Jurnal Kimia dan Pendidikan KOVALEN: Jurnal Riset Kimia Journal of Natural Sciences and Mathematics Research ALCHEMY Jurnal Penelitian Kimia PROSIDING SEMINAR NASIONAL Indonesian Journal of Chemical Analysis (IJCA) JKPK (Jurnal Kimia dan Pendidikan Kimia) Stannum : Jurnal Sains dan Terapan Kimia Jurnal Riset Teknologi Industri Jurnal Teras Fisika : Teori, Modeling, dan Aplikasi Fisika Jurnal Sains dan Edukasi Sains Jurnal Pengabdian kepada Masyarakat Nusantara Jurnal Ilmu Manajemen Terapan (JIMT) Jurnal Kimia dan Kemasan IJCR (Indonesian Journal of Chemical Research) Magistrorum et Scholarium: Jurnal Pengabdian Masyarakat Rafflesia Journal of Natural and Applied Sciences (RJNAS)
Cucun Alep Riyanto
Universitas Kristen Satya Wacana
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Journal : JURNAL KIMIA SAINS DAN APLIKASI

 1 
Validation of UV-VIS Spectrophotometric Methods for Determination of Inulin Levels from Lesser Yam (Dioscorea esculenta L.) Sandra Ayu Apriliyani; Yohanes Martono; Cucun Alep Riyanto; Mutmainah Mutmainah; Kusmita Kusmita
Jurnal Kimia Sains dan Aplikasi Vol 21, No 4 (2018): volume 21 Issue 4 Year 2018
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2211.725 KB) | DOI: 10.14710/jksa.21.4.161-165

Abstract

Validation of methods is necessary to ensure that the analytical methods used are appropriate and reliable. In this research, the determination of validation method and determination of inulin content from gembili bulb (Dioscorea esculenta L.). The method used for determining inulin levels is UV-Vis spectrophotometry. Based on the results obtained, the maximum wavelength determination of inulin levels is 520 nm. The method of analysis in this study meets the specified validation requirements. These parameters include the equation on the inulin raw curve y = 0.0045x +0.109 with the coefficient of determination 0.9992. The accuracy of the methods tested using the recovery test was 96.14-106.76% at concentrations of 25, 100, and 225 ppm. The intraday precision test is in the range of 2.67-6.82% at the same concentration as the accuracy test. Precision interday equal to 7.41% at concentration 225 ppm for 3 day analysis period. The values of LOD and LOQ are 3.58 ppm and 11.95 ppm, respectively. The concentration of inulin extract from gembili bulb is 840.44-12188.59 ppm.
Co-Authors A. Rindang Anggit Laksono Afif Afina Agung Rimayanto Gintu Agustinus Fritz Wijaya Andreas Setiawan Andri Purnomo Anggaran, Diki Anton Hermawan Ariesto, Yohanes Bangalino, Wira Wanti Bereka Meidelivia Raharjianti Betsy Felita Brotosudarmo, Tirza Widyamurti Christyawardana, Fidelis Tertius Aluh Dewi Kurnianingsih Arum Kusumahastuti Dwi Putri, Rima Angellika Eliana Prabalaras Elizabeth Elizabeth Ezra Kurniawan Fahmi Puteri Perdani Fidelis Tertius Aluh Christyawardana Fuady Hanief Harry Setiawan Hartati Soetjipto Hindra, Ronal Febria I Gede Kesha Aditya Kameswara Iga Permata Sari Ira Tyas Kurniasari Ira Tyas Kurniasari Jose D Michael Yonggulemba Jumiyati Jumiyati Kusmita Kusmita Laili Nurrohmah Lutiyono, Lutiyono Lydia Ninan Lestario Maria Githania Maya Krisnawati Marturia Ester Tumbelaka Michael Yonggulemba, Jose Dimas Mitra Krisdayanti Muhamad Syaiful Ampri Mutmainah Mutmainah Nicho Vernanda Wina Puspita November Rianto Aminu Nugroho, Tri Ariyanto Nur Aji Wibowo Nur Ayu Hidayati Nur Ayu Hidayati Pandu Krisnawan Pradana, Yehez Kiel Sandy Puspita, Nicho Vernanda Wina Ratu Anggriani Tangke Parung Resa Wulandari Riska Cahyaningtyas Samuel Yoga Saputra Sandra Ayu Apriliyani Sarawinda Hutagalung Sari, Iga Permata Sri Hartini Stefani, Delvi Anandhia Stefanus Agung Wicaksono sulistiyanto, sigit Teresa Febriyanti Tonia Nur Fitria Wawan Rustyawan Widhi Handayani Wiguna, Gede Arya Wijaya, Daniel Ricky Putra Wulandari, Gecia Ovi Yayi Suryo Prabandari Yehez Kiel Sandy Pradana Yemima Kristiani Yoga Andika Putra Yohanes Ariesto Yohanes Martono Yonggulemba, Jose D Michael Yos Saputra Halomoan