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All Journal Veterinary Biomedical and Clinical Journal
Lonameo, Bonick Kartini
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Veterinary

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Effects of Lycopene Treatments on Development, Hatchability, and Heart Rate of Zebrafish Embryos under Heat Stress Exposure Tuska, Habib Syaiful Arif; Salsabila, Odifiannisa Ayu; Lonameo, Bonick Kartini; Hardian, Andreas Bandang; Lesmana, M. Arfan; Yesica, Reza
Veterinary Biomedical and Clinical Journal Vol. 6 No. 1 (2024)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.01.1

Abstract

Lycopene, a potent antioxidant predominantly found in red-hued fruits and vegetables such as tomatoes, has been incorporated into embryonic maturation media in vitro, demonstrating enhancements in embryo quality. Despite these advancements, the specific effects of lycopene on Zebrafish embryo quality remain unexplored. This study aims to investigate the impact of lycopene (0.65 ppm) on the development of Zebrafish embryos, focusing on hatchability (HA), and heart rate (HR) under conditions of heat stress (HS). Fertile Zebrafish embryos at the gastrula stage were induced HS (30oC; 1 hour). The embryos will be segregated into two groups: those exposed to HS and nonHS (28oC). Each group will receive one of three treatments (four replicates): control / without lycopene (P1), 2μL lycopene (P2), and 4μL lycopene (P3). Observations using an inverted microscope was done every three up to 48 hours post-fertilization (hpf), and subsequently every five hours up to 96 hpf. Quantitative data obtained for HA and HR were analyzed with SPSS, One-Way ANNOVA (p<0.05). The antioxidants contained in lycopene can influence the speed and success of Zebrafish embryo development and HA exposed to HS. In addition, the antioxidant in lycopene can also suppress the HR frequency of Zebrafish embryos exposed to HS. However, more studies about the optimum dose, purity, and stability of the lycopene extract should be done to make a definitive conclusion.
Effects of Acetone Extract of Green Tea (Camellia sinensis) on Diameter, Viability, and Germinal Vesicle Breakdown Rate of Zebrafish Oocytes (Danio rerio) Exposed to Heat Stress as an Animal Model Tuska, Habib Syaiful Arif; Hidayah, Aaliyah Nurul; Lonameo, Bonick Kartini; Bello, Umar; Budiono
Veterinary Biomedical and Clinical Journal Vol. 6 No. 2 (2024):
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.02.2

Abstract

Global warming is characterized by increasing temperatures of the earth's atmosphere, ocean and land, resulting in extreme climate change. The effects of climate change will affect animal health through increased ambient temperature (heat stress). This study using Zebrafish as animal model like our previuous study because Zebrafish, have 70% identical genetic similarity to humans. In addition, Zebrafish have specific approval from the U.S. Food and Drug Administration for new drug discovery research. Heat stress negatively affects animal health through an increase in Reactive Oxygen Species (ROS), especially in the reproductive system because it causes a decrease in reproductive efficiency, such as follicular development, oocyte quality, and oocyte maturation. Antioxidant supplementation, namely green tea extract, is needed to reduce the negative effects of heat stress. The purpose of this study was to analyze the difference of 4 µl dose of green tea extract (Camellia sinensis) on the diameter, viability, and Germinal Vesicle Breakdown (GVBD) rate of Zebrafish oocytes at normal temperature (28℃) and with exposure to heat stress (32℃; 34℃). Zebrafish oocytes were collected and grouped into 2 (two) treatment groups including, non-heat stress group (28℃) and heat stress group (32℃; 34℃) with 6 repetitions.  Quantitative data were processed using SPSS software with Two Way ANOVA analysis.  The results of Two Way ANOVA showed that green tea extract supplementation had a positive effect on the diameter, viability, and GVBD of Zebrafish oocytes. This is indicated by the significance of the percentage of diameter (P=0.002), viability (P=0.003), and GVBD rate (P=0.000).
Lycopene Supplementation Mitigates Heat Stress-Induced Impairment of Embryonic Development, Survival, and Heart Rate in Zebrafish (Danio rerio) Residiwati, Gretania; Nararya, Winandana Parama; Ainurrahma, Hafidha; Lonameo, Bonick Kartini; Lesmana, Muhammad Arfan; Agustina, Galuh Chandra; Budiono; Hartono, Djoko; Hendrawan, Viski Fitri; Tuska, Habib Syaiful Arif; Sidi, Shehu
Veterinary Biomedical and Clinical Journal Vol. 8 No. 1 (2026): Vol. 8 No. 1 2026
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2026.008.01.1

Abstract

Fish at any change in aquaculture air temperature can affect fish survival, physiological conditions and immune response. The need to study the reaction of fish to sublethal temperatures (30-40oC) is an impetus because of global warming which can trigger heat stress (HS). Heat stress (HS) and its effects can occur in fish with any changes in the environment (physical or physiological), where heat can change the animal's normal homeostasis. Tomato (Lycopersicum esculentum), has been proven as a fruit that is rich in lycopene, which acts as a natural antioxidant. The purpose of this study was to determine the effect of lycopene supplementation on the process of external fertilization on embryo development, survival rate and heart rate of zebrafish (Danio rerio) at normal temperature (NHS) and with heat exposure (HS). The survival rate data obtained will then be analyzed using SPSS, with ANOVA and LSD tests (P ≤0.05). While the results of the statistical analysis test of heart rate, a non-parametric test was used using Kruskal-Wallis H, to find out whether there was a significant difference in the treatment; and the Mann-Whitney Test, to detect significant differences between groups (P ≤0.05). The effect of lycopene supplementation on the external fertilization process of zebrafish embryos gives a low survival rate and heart vibration of zebrafish embryos, which is thought to be caused by several factors, namely zebrafish genetics; storage method, dosage, and lycopene extraction method used in the research
Co-Authors Agustina, Galuh Chandra Ainurrahma, Hafidha Bello, Umar Budiono Hardian, Andreas Bandang Hartono, Djoko Hidayah, Aaliyah Nurul Lesmana, M. Arfan Lesmana, Muhammad Arfan Nararya, Winandana Parama Residiwati, Gretania Salsabila, Odifiannisa Ayu Sidi, Shehu Tuska, Habib Syaiful Arif Viski Fitri Hendrawan Yesica, Reza