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All Journal Journal of Multidisciplinary Science: MIKAILALSYS Asian Journal of Science, Technology, Engineering, and Art
Abah, Moses Adondua
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Agriculture, Biological Sciences & Forestry Arts Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Engineering Environmental Science Physics Social Sciences Other

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Journal : Journal of Multidisciplinary Science: MIKAILALSYS

 1 
Isolation and Molecular Characterisation of Chlorogonium sp. from Industrial Wastewater Abershi, Ajiduku Leyoa; Ikwebe, Joseph; Stephen, Ezeonu Chukwuma; Mafe, Alice Njolke; Abah, Moses Adondua
Journal of Multidisciplinary Science: MIKAILALSYS Vol 2 No 3 (2024): Journal of Multidisciplinary Science: MIKAILALSYS
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/mikailalsys.v2i3.3851

Abstract

Microalgae are photosynthetic, unicellular microorganisms also known as phytoplankton. They are small plant-like entities. In this research, the sample were collected from cement factory in a sterilised 20L container wrapped with foil paper and were transported down to Federal University, Wukari where it was kept in refrigerator at biochemistry laboratory. Blue-Green media (BG-11) was prepared. Wastewater containing Microalgae obtained from cement wastewater pond were cultivated in BG-11 medium to determine the growth of the organism. BG-11 medium contained macronutrients, trace metals and some vitamins which aid the growth of the organism. The wastewater sample containing with suspected microalgae was inoculated (inoculum ratio = 25%) and incubated under atmospheric CO2 at room temperature (30±2°C) in our laboratory for two to three weeks during the incubation period. In order to purify the isolates, the upper growth layer was first decanted into a freshly produced medium and then plated on BG-11 media that had been solidified with 1% agar-agar. For several of the cultures, growth on the agar plates continued for around three weeks. Following repeated sub-culturing, the emerging greenish colonies were re-emerged into a sterile BG-11 agar medium. In isolation of organism from the industrial cement waste water, the isolate was identified by morphological and molecular identification by extracting the DNA, run the electrophoretic analysis and PCR using primers for 18S rRNA eukaryotic microalgal and then run the sequence analysis. The results of this study obtained, indicated that, the electrophoretic result show the band has 1800-2000base pair and the organism isolated from the industrial cement waste water were chlorogonium sp. with a percent similarity of 78.65% and accession number of OR886595 based on data Gene Bank blast results.
Comparative Study on the Proximate Composition and Amino Acid Levels in Chanterelle (Cantharellus cibarius) and Wood Ear (Auricularia auricula) Mushroom Samples Adebisi, Arowora Kayode; Abah, Moses Adondua; Ugwuoke, Kenneth Chinekwu; Dooshima, Arowora Mercy; Ndoma, Odi Raphael; Okani, Precious Chizaram; Ominisan, Kimi Smith; Gbadebo, Adetoyi Moses; Olamilekan, Aliyu Najeeb; Kwame, Asare Bernard
Journal of Multidisciplinary Science: MIKAILALSYS Vol 2 No 3 (2024): Journal of Multidisciplinary Science: MIKAILALSYS
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/mikailalsys.v2i3.4052

Abstract

Mushrooms have been consumed by mankind for millennia. They are macrofungi with a distinctive fruiting body which can be hypogeous or epigeous, large enough to be seen with unaided eyes. They are not widely consumed due to lack of information on its nutritive value. Therefore, this research was carried out to compare the proximate gross energy composition as well as amino acids levels in two selected edible mushrooms (Cantharellus cibarius and Auricularia auricula). Fully matured species of Chanterelle mushroom (Cantherellus cibarius) and Wood ear mushroom (Auricularia auricula) were collected from different parts of Southern and Northern Cross River; Ikom and Bekwara precisely. The collected samples were air dried for 5 days and then stored in transparent polythene bag for analysis. The dried and stored mushroom were pounded to powdered form and then subjected to amino acid analysis using isocratic HPLC and proximate analysis for proximate composition using the standard methods of Association of Official Analytical Chemist (AOAC). The results of this research showed that chanterelle mushroom has high concentration of amino acids compared to Wood ear mushroom. However, there was significant differences (p<0.05) in the levels of isoleucine in both mushrooms. There were no significant differences in the proximate composition and gross energy levels of both mushroom species. Some of the amino acids detected in both mushrooms were: methionine, threonine, isoleucine, leucine, phenylalanine, valine, tryptophan, histidine, arginine, aspartic acid, glycine, alanine and glutamic acid. Based on the findings of this study, the two edible mushrooms were observed to have appreciable levels of gross energy and amino acids. However, Chanterelle mushroom had higher protein composition than wood ear. Hence, it can be concluded that Chanterelle mushroom has greater nutritive value and more suitable for consumption.
Co-Authors Abershi, Ajiduku Leyoa Abimbola, Adeyemi Bashirat Adebisi, Arowora Kayode Akpan, Rose Aniekan Arowora, Kayode Adebisi Chinenye, Chinweuba Rachael Dauda, Abdulrashid Dooshima, Arowora Mercy Edoka, Oduh Solomon Egwolo, Fueta Loveth Gbadebo, Adetoyi Moses Idris, Nancy Ifeanyi, Onyedinma Emmanuel Iheanacho, Chinedu Christian Ikwebe, Joseph Kwame, Asare Bernard Mafe, Alice Njolke Marvis, Alajemba Chinonso Mgbede, Timothy Najeeb, Aliyu Olamilekan Ndoma, Odi Raphael Nkwocha, Ikenna Light Nonye, Eze Constance Okani, Precious Chizaram Okpanachi, Victor Olamilekan, Aliyu Najeeb Olawale, Otitoju Ominisan, Kimi Smith Osagie, Asuelimen Steve Owei, Joy Ebimoboere Oyibo, Okpanachi Nuhu Ruyati, Samuel Sunny Samuel, Woyengibarakemi Ann Shadrach, Philip Stephen, Ezeonu Chukwuma Tatah, Silas Verwiyeh Timothy, Mgbede Timothy, Nwali Chukwuebuka Uchechukwu, Ukoh Daniel Ugwuoke, Kenneth Chinekwu Umezurike, Kingsley Chimuanya Yola, Anna