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Journal : Bioscientist : Jurnal Ilmiah Biologi

Identification of Gastrointestinal Protozoa of Sumatera Elephant (Elephas maximmus sumatranus) in Lombok Wildlife Park Situmorang, Fernando Jose Immanuel Clinton; Kholik, Kholik; Atma, Candra Dwi; Riwu, Katty Hendriana Priscilia; Dharmawibawa, Iwan Doddy; Pradana, Munawer
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11855

Abstract

The protozoan parasites have been reported to infect Sumatran elephants. Gastrointestinal protozoa could potentially be a factor in the decline in the Sumatran elephant population in Indonesia. This study aims to identify the presence of gastrointestinal protozoa in Sumatran Elephant (Elephas maximus sumatranus) in the Lombok Wildlife Park, North Lombok Regency, Indonesia. This research has used fresh fecal samples from 5 Sumatran elephants. The examination of feces samples using native, sedimentation, and floating methods. The research results have identified the presence of gastrointestinal protozoa in 2 of the 5 Sumatran elephant feces examined. The gastrointestinal protozoa found were Eimeria spp with dimensions of 16.30 x 20.93 μm and 25.21 x 38.49 μm.
Prevalence and Morphological Identification of Eimeria Species on Bali Cattle in Sumbawa District, West Nusa Tenggara Pradana, Munawer; Kurniawan, Muhammad Ahdi’; Berliana, Erprinanda Galuh; ‘Ilmi, Laily Ulya Nurul; Haddar, Mariyam Al; Dharmawibawa, Iwan Doddy
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13315

Abstract

This study aims to determine the prevalence and morphological identification of Eimeria spp. from Bali cattle feces on traditional farms in Sumbawa District. A total of 300 samples were identified and 238 samples were positive, resulting in a prevalence of Eimeria spp. of 79.33%. Based on age category, the highest prevalence of Eimeria spp. was found in cattle aged 6 months-2 years (88.76%; 79/89), followed by cattle aged  <6 months (88%; 88/100) and the lowest prevalence in cattle aged >2 years (63.96%; 71/111). Female Bali cattle had the highest prevalence of Eimeria spp. (80.55%; 145/180) than male Bali cattle (77.50%; 93/120). Based on livestock systems, the highest prevalence of Eimeria spp. was found in semi-intensive (89.15%; 74/83), then extensive (80.76%; 105/130) and the lowest in intensive (67.81%; 59/87). Six different Eimeria species were identified: E. bovis (33.19%), E. auburnensis (21.42%), E. zuernii (15.96%), E. alabamensis (12.18%), E. ellipsoidalis (10.50%), and E. canadensis (6.72%). Preventing and controlling coccidiosis infection in cattle can be done with good hygiene management.
Detection of HSP70 in Working Horses Using ELISA on Sumbawa Island Haddar, Mariyam Al; Pradana, Munawer; Kholik, Kholik; Supriadi, Supriadi; Satuman, Satuman
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15197

Abstract

Heat shock protein 70 also known as HSP70 is a protein that regulates in response to stress factors such as heat, exercise, and others. The current research work used enzyme-linked immunosorbent assay (ELISA) to measure HSP70 levels in working horses on Sumbawa Island. Blood was taken through jugular venipuncture whereas saliva was collected non-invasively via cotton swab, both were analyzed with ELISA. The results indicated different expressions of HSP70; saliva shows a significantly higher level of HSP70 (52.07 ± 25.25 ng/mL) than serum (43.71 ± 34.83 ng/mL) from the same animal. The observed differences might indicate a better representation of acute stress by saliva due to physiological rapid turnover compared to serum, which reflects an integration of cumulative stress exposure. Our finding thus proves that double monitoring of both biological matrices offers complementary information in assessing stress in working horses while taking salivary HSP70 into account as especially promising for the in-field welfare monitoring due to its non-invasive collection and sensitivity to immediate stress conditions.