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All Journal BIOEDUSCIENCE Jurnal Riset Biologi dan Aplikasinya Bioscientist : Jurnal Ilmiah Biologi
Mandiri, Listy Hasti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences

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Journal : BIOEDUSCIENCE

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Skrining Bakteri Lipolitik dari Tanah Pemakaman Mandiri, Listy Hasti; Rahayu, Triastuti; Tyastuti, Erma Musbita; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12686

Abstract

Background: The cemetery is an area for the burial of corpses so that active decomposition of bodies occurs. One of the decomposition bacteria that might be found is lipolytic bacteria because the human body comprises 21,33-32,51 % lipids. Explorations of lipolytic bacteria from cemeteries in Indonesia have not been found, so this research needs to be done. This non-experimental study aimed to select and identify lipolytic bacterial isolates from the Pracimaloyo cemetery, Surakarta. Method: A total of 36 bacterial isolates were selected for their lipolytic activity using tributyrin media. The lipolytic index determines lipolytic activity. Identification of lipolytic bacteria based on colonial morphology and Gram staining. Result: The study showed that 22.2% (8 isolates) showed a lipolytic positive, with the largest lipolytic index (LI) value of 2.5 (isolate P36). Bacterial colonies are circular-shaped, have entire edges, flat elevation, and are yellow or white. Gram staining results showed that the isolated is a group of Gram-negative bacteria in the form of coccus. Conclusion: The conclusion of this study suggests that bacterial isolates from Pracimaloyo cemetery that have potential lipolytic activity, which is likely to be from the genera Pseudomonas and Klebsiella, were obtained.
Co-Authors Ariyanti, Olivia Puja Fadilah, Fari Hardianto, Alden Ganendra Madhava Priya Jamil, Nazia Kistantia Elok Mumpuni Putri Agustina Seno, Hernandito Aryo Sofyan Anif Triastuti Rahayu Trio Ageng Prayitno, Trio Ageng Tyastuti, Erma Musbita Yasir Sidiq Zulperi, Dzarifah