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All Journal Bioeksperimen: Jurnal Penelitian Biologi BIOEDUSCIENCE Jurnal Pembelajaran dan Biologi Nukleus Community Empowerment Proceeding of International Conference on Biology Education, Natural Science, and Technology Berita Biologi
Tyastuti, Erma Musbita
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Physics Public Health Social Sciences

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Screening For Lipolytic Bacteria from Bonoloyo Cemetery, Surakarta Rini, Heni Sulistyo; Tyastuti, Erma Musbita; Siddiq, Yasir; Rahayu, Triastuti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4944

Abstract

A cemetery is a burial place managed by the government. Inside the TPU, the body is decomposed by microorganisms, one of which is lipolytic bacteria, because the human body is composed of about 12.5–13.60% lipids. Research on lipolytic bacteria from TPU in Indonesia has not been found, even though it has the potential to obtain lipolytic bodies. Therefore, this research was conducted to select lipolytic bacteria from TPU and provide simple assistance. A total of 45 bacterial isolates from TPU Bonoloyo Surakarta were selected for their lipolytic activity using tributyrin agar media. Lipolytic activity was determined using the lipolytic index (LI). Bacterial isolates with lipolytic potential were identified based on colony morphology and Gram staining. The results showed that 30 isolates (67%) showed lipolytic activity, with the highest LI value of 5.43 (BLB 9) after 2x24 hours of incubation. Bacterial isolates that have lipolytic activity are white colonies with circular shapes. The results of Gram staining showed that the bacterial isolates belonged to a group of Gram-negative bacteria in the form of cocci. The conclusion put forward is that public cemeteries (TPU) have the potential to store isolates of lipolytic bacteria dominated by Gram-positive bacteria by as much as 57%
Improving marketability and quality of Sulemu soy milk: A community engagement approach to product diversification Rahayu, Triastuti; Nurcahyanto, Guntur; Aryani, Ima; Suryani, Titik; Tyastuti, Erma Musbita; Sari, Siti Kartika; Maimun, Muhammad Halim; Ripdianti, Arum Dyah; Hardianto, Alden Ganendra Madhava Priya
Community Empowerment Vol 10 No 6 (2025)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/ce.13197

Abstract

Original "SuleMu" soy milk currently has limited shelf life without refrigeration. Therefore, it is crucial to assist partners in enhancing product competitiveness and diversification. The primary objectives of this community engagement activity were to improve SuleMu's marketability through the addition of new flavors and the provision of nutritional value analysis, as well as to enhance product quality by developing derivative products such as soyghurt and frozen yogurt. The activity stages included: (1) mentoring for the diversification of SuleMu derivative products, (2) nutritional value analysis of derivative products, (3) designing product labels compliant with standards, and (4) conducting a market survey on consumer preferences for SuleMu derivative products. Results indicate that SuleMu is now available in new flavors: melon, chocolate, and strawberry. The nutritional content per 100 grams is 3.4–3.5 grams of soluble protein, 3.3–3.7 grams of fat, 5–5.7 grams of reducing sugars, 2.6–3.6 grams of crude fiber, and approximately 39–40 mg of calcium. SuleMu production increased from 5 kg of soybeans to 6 kg per day. Furthermore, consumers showed high interest in the "Soyoghurt" frozen yogurt derivative product, with 42.4% being very interested and positive evaluations regarding taste (36.1%) and affordable price (29.2%). Overall, this community engagement activity successfully increased SuleMu's production by 17%, and soyghurt demonstrates significant potential for development as a highly competitive new innovation.
Phosphate Solubilisation Index and Antagonism Potential of Frangipani Tree Rhizosphere Bacterial Isolates from Cemetery Febriyanti, Vika; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Frangipani trees (Plumeria acuminata) are commonly found in cemeteries. Cemeteries are used as a location for interment so that the corpse actively decomposes to provide soil minerals that encourage the formation of microbes, including bacteria. Bacteria around the roots of frangipani trees are thought to have potential as plant growth-promoting rhizobacteria (PGPR). Based on reference searches, there has been no research on frangipani tree rhizosphere bacteria from burial grounds. The aim of this study was to investigate the phosphate-dissolving and blast disease-causing Pyricularia oryzae fungus-inhibiting properties of frangipani tree rhizosphere bacteria from Pracimaloyo cemetery, Surakarta. A total of 39 isolates of frangipani tree rhizosphere bacteria were tested for their ability to dissolve phosphates using PKA media (Pikovskaya), while the antagonism test against the fungal pathogen Pyricularia oryzae used the dual assay method. The results of the study showed that 15% of the rhizosphere bacteria of the frangipani tree were able to dissolve phosphate, and 33% of the isolates were able to inhibit the growth of the fungus Pyricularia oryzae with an inhibitory power of more than 40%. Based on the results, the rhizosphere bacterial isolates from Pracimaloyo TPU show the characteristics of a possible PGPR.
Isolation and Characterization of Cellulolytic Bacteria from Bonoloyo, Cemetery Risnasari, Wanda Datik; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

A public cemetery (TPU) is land provided by the government to bury bodies. The culture of funerals in Indonesia is varied but generally involves being buried in the soil with wrapped clothes, covered wooden boards, and tombs. The material contains cellulose that will be broken down by cellulolytic microorganisms, including bacteria. The exploration of the cellulolytic bacteria of TPU in Indonesia has not been carried out, so this study aims to investigate and identify cellulose bacteria isolates from TPU. This research is a non-experimental study for the isolation of bacteria in the soil of TPU Bonoloyo, Surakarta. A selection of cellulite bacteria is grown in CMC media. Isolates that show cellulolytic activity are identified by observing colonial morphology and Gram coloring. Selection results of cellulolytic bacteria obtained isolates with cellulolytic index (IS) including a high category of 1 isolate (B14), a medium category of 3 isolates (B16, B27, and B45), and 9 low category isolates. The study concluded that 28.8 % showed cellulolytic activity with irregular morphological forms of white dyeing on flat surfaces with flat edges and dominated by gram-negative coccyx-shaped.
Isolation and Identification of Soil Bacteria in Pracimaloyo Public Cemetery, Kartasura Adityaradja, Bagas; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

In The Pracimaloyo Public Cemetery, the body’s decomposition occurs continuously, producing soil nutrients and minerals that affect the growth of bacteria. Bacterial population data from the cemetery area is still very limited, whereas cemeteries hold the risk of contamination with pathogenic bacteria. This research aims to find out how the population and diversity of bacteria in the Pracimaloyo Public Cemetery. Soil samples were taken from the Pracimaloyo cemetery at 2 locations (blocks 8 and 18), each at 20 and 50 cm depth. Soil samples are inoculated in Nutrient Agar media using the spread plate method. After 48 hours, colony counting, colony morphology, and gram staining observations were carried out. The rate of soil bacterial populations in blocks 8 and 18 at a depth of 20 cm was 4.23 × 107 CFU/g and 9.79 × 107 CFU/g, while at depths of 50 cm, it was 1.94 × 107 CFU/g and 1.92 × 107 CFU/g. The morphology of the bacterial colonies is dominated by circular shape, entire margin, flat elevation, and white color. 20 isolates are gram-negative and 16 isolates are gram-positive, the cell form is dominated by the bacillus.
Screening and Identification of Cellulolytic Bacteria from Public Cemetery Soil Syarifah, Siti Nur; Rahayu, Triastuti; Tyastuti, Erma Musbita; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11745

Abstract

Background: A public cemetery (TPU) is where bodies are buried. Before being buried, the body is wrapped in a shroud or full clothes and sometimes put in a coffin. These materials contain cellulose, a substrate for cellulolytic microorganisms, including bacteria, to decompose. Exploration of cellulolytic bacteria in TPU has not been found, even though TPU holds potential cellulolytic bodies. This study aims to determine the cellulolytic potential and identify bacterial isolates from TPU. Methods: 36 bacterial isolates tested for cellulolytic potential have been isolated from TPU Pracimaloyo Kartasura, Sukoharjo. Selection of cellulolytic bacteria used selective Carboxymethyl Cellulose (CMC) media dripped with Congo red 0.1%, while identification was based on colony morphology and Gram staining. Results: This study obtained one isolate (2.78%) showing high cellulolytic activity with a cellulolytic index (IS) 7, namely P14, 24 isolates (66.67%) in the "moderate" category, four isolates (11.11%) with category "low," and seven isolates (19.44%) did not show cellulolytic ability. Isolates cellulolytic positive have a shiny white colony color, entire edges, raised elevation, and belong to the Gram-negative coccus form. Conclusions: From the results of this study, it can be concluded that public burial sites (TPU) harbor potential cellulolytic bacteria.
Skrining Bakteri Lipolitik dari Tanah Pemakaman Mandiri, Listy Hasti; Rahayu, Triastuti; Tyastuti, Erma Musbita; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12686

Abstract

Background: The cemetery is an area for the burial of corpses so that active decomposition of bodies occurs. One of the decomposition bacteria that might be found is lipolytic bacteria because the human body comprises 21,33-32,51 % lipids. Explorations of lipolytic bacteria from cemeteries in Indonesia have not been found, so this research needs to be done. This non-experimental study aimed to select and identify lipolytic bacterial isolates from the Pracimaloyo cemetery, Surakarta. Method: A total of 36 bacterial isolates were selected for their lipolytic activity using tributyrin media. The lipolytic index determines lipolytic activity. Identification of lipolytic bacteria based on colonial morphology and Gram staining. Result: The study showed that 22.2% (8 isolates) showed a lipolytic positive, with the largest lipolytic index (LI) value of 2.5 (isolate P36). Bacterial colonies are circular-shaped, have entire edges, flat elevation, and are yellow or white. Gram staining results showed that the isolated is a group of Gram-negative bacteria in the form of coccus. Conclusion: The conclusion of this study suggests that bacterial isolates from Pracimaloyo cemetery that have potential lipolytic activity, which is likely to be from the genera Pseudomonas and Klebsiella, were obtained.
EKOGENOTOKSISITAS LIMBAH CAIR BATIK DAN EFEK ANTIMUTAGENIK Lemna minor TERHADAP ERITROSIT IKAN NILA (Oreochromis niloticus) Tyastuti, Erma Musbita; Astirin, Okid Parama; Sunarto, Sunarto
Bioeksperimen: Jurnal Penelitian Biologi Vol 2, No 2: September 2016
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v2i2.2490

Abstract

Limbah cair batik di Solo sebagian besar dibuang langsung ke perairan tanpa diolah terlebih dahulu dan menyebabkan pencemaran air. Kandungan logam berat di dalam limbah cair batik dapat memicu efek genotoksik seperti pembentukan mikronukleus. Lemna minor berpotensi sebagai antimutagen dan mencegah pembentukan mikronukleus karena mengandung senyawa aktif seperti karoten dan asam amino. Penelitian ini bertujuan untuk mengetahui ekogenotoksisitas limbah cair batik dan efek antimutagenik Lemna minor terhadap eritrosit ikan nila. Penelitian dilakukan di Laboratorium Biologi UMS dengan pemaparan limbah cair batik 0ppm/L, 2500 ppm/L, 5000 ppm/L dan 7500 ppm/L terhadap 2 kelompok ikan nila dengan diet pelet dan Lemna minor. Hasil penelitian menunjukkan bahwa paparan limbah cair batik memicu pembentukan mikronukleus dengan frekwensi tertinggi pada konsentrasi paparan 7500 ppm/L. Lemna minor juga terbukti memiliki potensi antimutagenik karena mampu menekan frekwensi mikronukleus lebih rendah dibandingkan diet pelet.
BIODEGRADASI PLASTIK OLEH CAMPURAN BAKTERI LIPOLITIK DI DALAM KOLOM WINOGRADSKY Rahayu, Triastuti; Sidiq, Yasir; Harismah, Kun; Santhyami, Santhyami; Agustina, Putri; Haryanto, Haryanto; Tyastuti, Erma Musbita; Aurelia, Fanisha; Sabdina, Berlian Achya Putri
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.5706

Abstract

Plastik menjadi masalah serius karena sangat lambat didegradasi. Oleh karena itu banyak usaha dilakukan untuk mempercepat degradasinya tetapi belum menunjukkan hasil yang signifikan. Salah satu upaya yang dicoba adalah menggunakan bakteri lipolitik. Tujuan penelitian ini adalah menguji kemampuan degradasi plastik menggunakan campuran bakteri lipolitik dalam kolom Winogradsky. Penelitian ini merupakan penelitian eksperimen dengan 2 faktor perlakuan. Faktor 1; jenis plastik (P1/kresek hitam putih) dan (P2 /bungkus mie instan). Faktor 2; waktu inkubasi (W1/1,5 bulan) dan (W2/3 bulan). Plastik diuji degradasi menggunakan campuran bakteri B9 dan B28 dalam kolom Winogradsky. Data yang dianalisis berupa uji antagonisme dan identifikasi bakteri, persen pengurangan berat kering, SEM, dan FTIR. Kedua isolat tidak menunjukkan antagonisme. Isolat B9 teridentifikasi Staphylococcus saprophyticus, sedangkan B28 adalah Bacillus amyloliquifaciens. Persen pengurangan berat kering P1 lebih besar dibanding P2 sebesar 5,11 (4,91%) dan 4,99 (3,99%) masing-masing untuk 1,5 dan 3 bulan. Permukaan P2 terbentuk kerutan lebih banyak dan terdapat cekungan yang dalam di beberapa titik, sedangkan P1 masih tampak lebih halus. Hasil analisis FTIR P2 menunjukkan  munculnya spektra senyawa amina, alkane dan CO2. Campuran bakteri Staphylococcus saprophyticus dan Bacillus amyloliquifaciens mempunyai kemampuan mendegradasi plastik dalam kolom Winogradsky dan plastik 2 menunjukkan tingkat degradasi lebih lanjut dibandingkan plastik 1.
Co-Authors Adityaradja, Bagas Aurelia, Fanisha Febriyanti, Vika Guntur Nurcahyanto Hardianto, Alden Ganendra Madhava Priya Haryanto Haryanto Ima Aryani Kun Harismah Maimun, Muhammad Halim Mandiri, Listy Hasti Okid Parama Astirin Putri Agustina Rini, Heni Sulistyo Ripdianti, Arum Dyah Risnasari, Wanda Datik Sabdina, Berlian Achya Putri Santhyami Santhyami Sari, Siti Kartika Siddiq, Yasir Sunarto Sunarto Syarifah, Siti Nur Titik Suryani Triastuti Rahayu Yasir Sidiq