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All Journal Jambura FIsh Processing Journal Jurnal Abdi Insani Agrokompleks BIOEDUPAT: Pattimura Journal of Biology and Learning Biofaal Journal Jurnal Ilmu Pendidikan Indonesia
Metungun, Jakomina
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PENGARUH EKSTRAK ETANOL DAUN MANGKOKAN (Nothopanax scutellarium) YANG DIMANFAATKAN SEBAGAI ANTIBAKTERI DARI BAKTERI Vibrio sp Hitijahubessy, Hendro; Hanoatubun, Marthinus Imanuel Halaay; Fangohoi, Sesilia; Tumiwa, Bruri Berel; Metungun, Jakomina; Madubun, Usman
Biofaal Journal Vol 5 No 2 (2024): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i2pp091-099

Abstract

One of the pathogenic bacteria found in aquatic environments is Vibrio sp., which causes vibriosis. The use of mangkokan leaf extract as an antibacterial agent against Vibrio has not been studied before. The aim of this research was to evaluate the antibacterial activity of mangkokan leaf extract in inhibiting the growth of Vibrio sp. The antibacterial test in this study used the disc diffusion method. In addition, the study also conducted tests for yield and phytochemical analysis. The results showed that mangkokan leaf extract at concentrations of 100% and 75% exhibited moderate antibacterial activity, with average inhibition zone diameters of 8.5 mm and 6.35 mm, respectively. The yield of the ethanol extract from mangkokan leaves was 10.2%. The mangkokan leaf extract contains secondary metabolites such as flavonoids, steroids, alkaloids, tannins, and saponins.
FEEDING OF ULVA LACTUCA EXTRACT AGAINST THE IMMUNE PARAMETERS AND SURVIVAL OF DUCK GROUPER FISH (Cromileptes altivelis) Metungun, Jakomina; Beruatjaan, Mariana; Hatijahubessy , Hendro; Putnarubun, Cenny
Jurnal Ilmu Pendidikan Indonesia Vol 13 No 2 (2025): JURNAL ILMU PENDIDIKAN INDONESIA
Publisher : Master of Science Education Program, Postgraduate Program of Cenderawasih University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/jipi.v13i2.4699

Abstract

High mortality in humpback grouper infected with Vibrio alginolitycus disease is a major problem in farming activities. The aim of this research was to examine the effect of oral administration of Ulva lactuca extract in feed to improve the non-specific immune system and survival of Vibrio alginolitycus. This research was carried out from July to December 2023 at the Tual State Fisheries Polytechnic Campus. The method used was a Completely Randomized Design (CRD) method with 3 treatments, namely treatment A Ulva extract 1 gr/kg feed, treatment B 2 gr/kg feed and treatment C 3 gr/kg feed, plus 2 treatments as controls, namely K( +) was not fed Ulva extract, was challenged with Vibrio alginolyticus bacteria 108 CFU/ml and K (-) was not fed Ulva extract, was not challenged but was injected with PBS. The results of the study showed that oral administration of Ulva lactuca extract containing antibacterial compounds was thought to be able to increase the non-specific body defense system of humpback grouper, characterized by increased levels of hemoglobin (Hb), total erythrocytes, total lymphocytes found in treatment C at a dose of 3 g/kg feed. and the best survival in treatments B and C was 80%.
Kemampuan antibakteri daun rambutan (Nepheliium lappaceum Linn) terhadap bakteri Vibrio sp. secara in vitro Hitijahubessy, Hendro; Sianturi, Anggreni; Warbal, Triyune Christovena; Hanoatubun, Marthinus Imanuel Halaay; Madubun, Usman; Metungun, Jakomina
Agrokompleks Vol 25 No 2 (2025): Vol 25 No 2 (2025): Agrokompleks Edisi Juli
Publisher : Politeknik Pertanian Negeri Pangkajene Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51978/japp.v25i2.936

Abstract

Vibrio sp. is an opportunistic pathogenic bacterium that causes vibriosis in fish, shrimp, and seaweed. The use of synthetic antibiotics is everyday; however, resistance is difficult to prevent. The advantages of using natural antibiotics include their availability, environmental friendliness, and cost-effectiveness. This study aimed to utilize a natural material, namely rambutan (Nephelium lappaceum L.) leaf extract, as a candidate to be tested for its antibacterial activity against Vibrio sp. A phytochemical analysis of the rambutan leaf extract was conducted to identify the presence of bioactive compounds that inhibit bacterial growth. The extraction was performed through maceration using 70% ethanol as the solvent. The extract was then diluted in a series of concentrations ranging from 100% to 3.125%. Antibacterial activity was tested using the disk diffusion method on Thiosulfate Citrate Bile Salt Sucrose (TCBS) agar medium. The results showed a potent inhibitory effect at the 100% extract concentration, with an inhibition zone diameter of 24.675 ± 0.53 mm. Potent inhibition was also observed at 75% and 50% extract concentrations, with inhibition zones of 19.95 ± 1.56 mm and 19.225 ± 4.63 mm, respectively. Phytochemical analysis of the rambutan leaf extract revealed the presence of several bioactive compounds, including alkaloids, steroids, flavonoids, saponins, and tannins. The study concluded by calculating the extract yield to assess the efficiency of rambutan leaf extraction using ethanol, yielding 7.16 ± 0.14%.
Implementation of project-based learning in Aquaculture Course at Tual State Polytechnique of Fisheries Sahusilawane, Helena Afia; Metungun, Jakomina; Zahri, Abdul; Lutur, Ellen Marta; Fitrinawati, Henny
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 5 No 1 (2025): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/bioedupat.v5.i1.pp265-271

Abstract

Project-based learning model emphasizes practical experience and real projects. By implementing this learning model, skills that are much needed in society and the world of work today can be developed well in higher education. The Aquaculture Technology study program at the Politeknik Perikanan Negeri Tual must prepare graduates who are competent at work. The aquaculture course offers location planning concepts, culture methods, feeding management, water quality management, disease control, harvesting, packing and transportation of harvested produce. These concepts can be learned by applying a project-based learning model using the Recirculating Aquaculture System (RAS) for fish culture. Thus, this study was aimed to analyze the effectiveness of project-based learning model implementation to improve the student learning outcomes in aquaculture course. In this study, seven students of 4th semester from the Aquaculture Technology Study Program was selected. The learning outcomes assessed include the cognitive domain using pre-test and post-test questions, while affective and psychomotor assessment used the 21st century skills, literacy skills, life skills, reports and presentations. All data were analyzed descriptively. The results indicate that the project-based learning model is effectively applied in aquaculture course, as shown by the average initial test score of 55.29 and an increase in the final test score of 80.86; 21st century skills of 81.79; literacy skills of 74.95; life skills of 75.49; reports and presentations amounted to 74.14. In lecture activities, students are productive and creative in developing knowledge, attitudes, and skills in an integrated manner.
ANALISIS KEMAMPUAN DARI EKSTRAK ETANOL RUMPUT LAUT Ulva lactuca SEBAGAI ANTIBAKTERI Vibrio sp. DAN KAJIAN FITOKIMIANYA Metungun, Jakomina; Beruatjaan, Mariana Yerminal; Hitijahubessy, Hendro; Tamher, Endang
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp100-107

Abstract

An analysis of the antibacterial ability of Vibrio sp. has been carried out from the ethanol extract of Ulva lactuca seaweed and its phytochemical study. Analysis of the antibacterial ability of Vibrio sp. using the disk diffusion method was the main thing carried out in this research, then the extract yield was calculated from the maceration process of Ulva lactuca seaweed, as well as phytochemical analysis to support the antibacterial ability of Ulva lactuca seaweed against the bacteria Vibrio sp. The results of the extraction of Ulva lactuca seaweed with ethanol solvent obtained a yield of 3.67%. The results showed a moderate antibacterial activity of Vibrio sp. from Ulva lactuca seaweed extract at a concentration of 100% was 17.3 mm. Weak antibacterial activity was at a concentration of 75% of 12.525 mm and extracts below a concentration of 75% did not have inhibitory power against Vibrio sp bacteria. Phytochemical studies of Ulva lactuca seaweed found secondary metabolite compounds in the form of alkaloids, flavonoids, steroids, tannins and saponins. Based on the results of this research, it is hoped that the antibacterial ability of Vibrio sp. can be further investigated from ethanol extract of Ulva lactuca seaweed in vivo.
PELATIHAN PEMBUATAN PAKAN BUATAN DARI TEPUNG Sargasum sp. KEPADA PEMBUDIDAYA TERIPANG DI DESA TAAR-KOTA TUAL Sahusilawane, Helena Afia; Beruatjaan, Mariana Y.; Syahailatua, Diana Y.; Metungun, Jakomina
Jurnal Abdi Insani Vol 12 No 4 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i4.2308

Abstract

Teripang merupakan salah satu komoditas perikanan yang sangat bernilai ekonomis dan bermanfaat untuk kesehatan. Oleh karena itu, diperlukan upaya budidaya yang baik dan tepat sehingga dapat memenuhi syarat ekspor teripang. Desa Taar Kepulauan Kei telah mengembangkan upaya budidaya teripang pada skala usaha mikro. Namun, permasalahan utama adalah ukuran tubuh yang kecil sehingga sulit menembus  pasaran. Hal ini disebabkan penyediaan  pakan yang terbatas. Oleh karena itu, pembudidaya dan pengusaha harus dilatih untuk membuat pakan yang tepat melalui kegiatan pengabdian masyarakat. Tujuan dari kegiatan ini adalah untuk meningkatkan pengetahuan dan keterampilan masyarakat dalam menyediakan kualitas dan kuantitas pakan yang tepat serta mampu menerapkannya dalam pembudidayaan teripang. Dalam kegiatan ini, dilakukan metode secara praktis pembuatan pakan yang dapat dijangkau oleh masyarakat yakni pelatihan dan pendampingan pembuatan pakan dari tepung Sargasum sp., dan lamun yang dikombinasikan dengan bahan lainnya. Selanjutnya dilakukan aplikasi pakan yang telah dibuat ke teripang yang dibudidayakan. Dalam kegiatan sosialisasi dan       pelatihan, peserta menunjukkan antusiasime untuk mengembangkan diri. Hal ini ditunjukkan dengan keseriusan mengikuti materi, diskusi dan keikutsertaan dalam pelatihan pembuatan pakan sampai aplikasi pemberian pakan ke teripang yang dibudidayakan. Hasil evaluasi dengan kuisioner juga menunjukkan bahwa materi yang disampaikan relevan dengan kebutuhan dan dapat dipahami oleh peserta. Berdasarkan hasil kuisioner tentang kebermanfaatan kegiatan, sebanyak 96,87% peserta menyebutkan bahwa kegiatan ini sangat bermanfaat. Sedangkan rerata nilai pemahaman dan keterampilan setelah pelatihan sebesar 96,87%. Pelatihan pembuatan pakan buatan bagi pembudidaya teripang di Desa Taar menunjukkan kebermanfaatan yang efektif bagi peningkatan pengetahuan dan keterampilan.
Pigmen Klorofil Pada Mikroalga Coccolithophore sp. Hasil Kultivasi Pada Skala Laboratorium Ngabalin, Daniel; Putnarubun, Cenny; Metungun, Jakomina; Ngabalin, Dewy Rosi
Jambura Fish Processing Journal Vol 7, No 2 (2025): VOLUME 7 NOMOR 2, JULY 2025
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/jfpj.v7i2.33201

Abstract

Microalgal pigments are important bioactive compounds with potential applications in the food, pharmaceutical, and cosmetic industries. Coccolithophore sp., a type of marine microalga, is known to contain pigments such as carotene, chlorophyll, and xanthophyll. This study aims to identify the types of pigments present in Coccolithophore sp. using column chromatography. The extraction process was carried out using 90% acetone, followed by pigment separation with a stepwise eluent ratio of hexane:acetone (4:1 to 1:4). The separation results revealed the presence of carotene (yellow), chlorophyll a (dark green), chlorophyll b (light green), and xanthophyll (light yellow) pigments. Elution times varied depending on the polarity of each compound. These results demonstrate the potential of Coccolithophore sp. as a natural pigment source for environmentally friendly industrial applications.
Co-Authors Beruatjaan, Mariana Beruatjaan, Mariana Y. Beruatjaan, Mariana Yerminal Fangohoi, Sesilia Fitrinawati, Henny Hanoatubun, Marthinus Imanuel Halaay Hatijahubessy , Hendro Hendro Hitijahubessy Lutur, Ellen Marta Madubun, Usman Ngabalin, Daniel Ngabalin, Dewy Rosi Putnarubun, Cenny Sahusilawane, Helena Afia Sianturi, Anggreni Syahailatua, Diana Y. Tamher, Endang Tumiwa, Bruri Berel Warbal, Triyune Christovena Zahri, Abdul