Indah Wijayanti
Department Of Animal Nutrition And Feed Science Technology, Faculty Of Animal Science, Bogor Agricultural University, Bogor, 16680, Indonesia

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Journal : Buletin Peternakan

The Use of Lactic Acid Bacteria as Ruminant Probiotic Candidates Based on In Vitro Rumen Fermentation Characteristics Roni Ridwan; Win Ariga Bungsu; Wulansih Dwi Astuti; Rohmatussolihat Rohmatussolihat; Nurul Fitri Sari; Rusli Fidriyanto; Anuraga Jayanegara; Indah Wijayanti; Yantyati Widyastuti
Buletin Peternakan Vol 42, No 1 (2018): BULETIN PETERNAKAN VOL. 42 (1) FEBRUARY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i1.23317

Abstract

This research was conducted to select, to identify LAB isolates and to investigate the effects of the LAB as probiotics candidate in the rumen fermentation. Nine isolates exhibited the potency as candidate probiotics for cattle. The experiment was arranged in randomized block design with ten treatments and three different times of in vitro as a block. The substrate consisted of 70% forage and 30% concentrate proportion. The substrate was incubated at 39oC using serum bottle of 100 ml capacity for fermentation. Approximately 0.75 g of substrates was put inside the serum bottle glass and filled with 73 ml of buffered rumen fluid and 2 ml of LAB inoculant. Gas production was measured every 2, 4, 6, 8, 10, 12, 24, 48 and 72 h of the incubation period. Gas production kinetic was estimated by the Ørskov’s equation. The LAB with the highest gas production, as probiotics candidate, were identified using partial 16S rDNA sequence. The results of this research indicated that nine LAB produced high gas production in the range of 193-198 ml compare to that of control (173 ml). The addition of LAB in rumen fermentation resulted in digestibility 65-75%, organic matter digestibility 51-73%, and 6.67-6.68 pH. Based on the molecular identification, 8 isolates are Lactobacillus plantarum and 1 of uncultured bacteria. The LAB strain 32 L. plantarum showed the best for a ruminant probiotic candidate based on the in vitro rumen fermentation characteristic.
THE EVALUATION OF SILICA+ SUPPLEMENTATION IN DIFFERENT TYPE OF DIET ON PERFORMANCE, NUTRIENT RETENTION AND THE ECONOMIC VALUE OF BROILERS DIET Irfan Anshory; Sumiati Sumiati; Indah Wijayanti
Buletin Peternakan Vol 41, No 4 (2017): BULETIN PETERNAKAN VOL. 41 (4) NOVEMBER 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i4.24976

Abstract

This study aimed to evaluate the effect of silica+ supplementation (Si) in different types of diet on performance, nutrient retention (energy, protein, fat), and the economic value of broiler diet. The treatment diets are 1. HN (high nutrient), 2. HN+Si, 3. LN (low nutrient), 4. LN+Si, 5. HNRB (high nutrient contained rice bran) and 6. HNRB+Si. A number of 1440 unsexed day old chicks (CP707 strains) were arranged in a com­pletely randomized design consisted of 6 treatments and each treatment was replicated six times.  The results showed that silica+ supplementation in HNRB and LN diets did not affect the performance and nutrient retention. The HNRB diet produced low fat retention, high FCR during starter period, and lower body weight during starter and grower period (P<0.05). The LN diet resulted high FCR during starter period (P<0.05), however it had no effects on the body weight. Silica+ supplementation on HN diet decreased the FCR by 8.09% during the grower period (P<0.05). Besides, it increased the fat retention and true metabolizable energy corrected by nitrogen (TMEn) by 1.30% and 4.41%, respectively. In general, silica+ supplementation was applied on the high nutrient diet during the grower period (22 to 35 days of ages) was effective. HN+Si produced the highest economic value diet in terms of income over feed and chick cost (IOFCC) increasing by 11.07% and relative feed cost (RFC) decreasing by 4.16%.
Effect of Different Ratio Omega-3 and Omega-6 in Total Mix Ration on Productive Performance, Blood Metabolites and Estrous Characteristic of Ewes Yusti Pujiawati; Lilis Khotijah; Asep Sudarman; Indah Wijayanti
Buletin Peternakan Vol 42, No 4 (2018): BULETIN PETERNAKAN VOL. 42 (4) NOVEMBER 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i4.29254

Abstract

This study was conducted to compare the effect of different ratio of omega-3:omega-6 in total mix ration to blood metabolites and reproductive performance of ewes. A total of 25 young ewes were randomly assigned to five experimental groups and five replications : R0 (without omega-3:omega-6), R1 (omega-3:omega-6 1:8), R2 (omega-3:omega-6 1:6), R3 (omega-3:omega-6 1:3), R4 (omega-3:omega-6 1:2). The parameters measured were feed intake, productive performance, blood glucose and cholesterol pre-mating period. Parameters of characteristic estrous measured were onset of estrous, length of estrous and estrous response. The result showed that ratio of omega-3 and omega-6 1:2 have a greatest daily weight gain and feed efficiency. Ratio of omega-3 and omega-6 1:2 have blood glucose lowest, but highest blood cholesterol. Addition of omega-3 cause to delays onset of estrous. The conclusion is greatest length of estrous, estrous response and pregnacy rate on ratio of omega-3 and omega-6 1:2 in ration.