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Cytotoxicity Test of Green Betel Leaf Ethanol Extract (Piper Betle L.) Using Shrimp Larvae (Artemia Salina L.) Using The Brine Shrimp Lethality Test (BSLT) Method Ismail, Mardhiyah Adawiyah; Vitayani, Sri; Nur Aulia Amir; Yusriani Mangarengi; Marzelina Karim; Galagher, Mahry
International Journal of Health and Pharmaceutical (IJHP) Vol. 5 No. 1 (2025): February 2025 (Indonesia - Turkey - Malaysia - Australia - Iran)
Publisher : CV. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51601/ijhp.v5i1.407

Abstract

The Brine Shrimp Lethality Test (BSLT) method is a preliminary method for screening anticancer compounds by looking at the cytotoxic effect of a sample that will be tested on Artemia salina shrimp larvae. This study aims to evaluate the cytotoxic effect of ethanol extract of green betel leaves (Piper betle L.) on Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method. Extraction was carried out by maceration method using 96% ethanol, and phytochemical screening to show the presence of secondary metabolite on green betel leef. Cytotoxicity tests were carried out at five extract concentrations (concentrations of 50 ppm, 100 ppm, 250 ppm, 500 ppm, and 750 ppm). Based on the results of the research carried out, it can be concluded that the results show that the percentage of larvae deaths ranges from 30-80% and the highest mortality was obtained at a concentration of 750 ppm with a total of 26 larval deaths, this death was in line with the increase in concentration. The Lethal Concentration (LC50) value obtained was 121.3317 ppm. In conclusion, green betel leaf extract contains secondary metabolite compounds which cause cytotoxic effects on Artemia salina Leach shrimp larvae with a mortality percentage of 30-80% and the LC50 value is 121.3317 ppm.
Identifikasi Bakteri pada Peralatan Medis Sebelum dan Sesudah Operasi Bersih di RS Ibnu Sina Ramadhan, M Arladen; Yusriani Mangarengi; Dian Fahmi Utami; Berry Erida Hasbi; Marzelina Karim
Fakumi Medical Journal: Jurnal Mahasiswa Kedokteran Vol. 5 No. 1 (2025): Maret
Publisher : Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/fmj.v5i1.532

Abstract

Infeksi nosokomial merupakan salah satu masalah serius di rumah sakit, terutama di ruang operasi yang memiliki risiko tinggi terhadap kontaminasi bakteri. Penelitian ini bertujuan untuk mengidentifikasi jenis bakteri yang terdapat pada peralatan medis sebelum dan sesudah pelaksanaan operasi bersih di ruang operasi Rumah Sakit Pendidikan Ibnu Sina YW UMI Makassar. Penelitian ini menggunakan metode deskriptif laboratorik dengan pendekatan makroskopis dan mikroskopis. Pengambilan sampel dilakukan dengan metode usap pada peralatan medis utama (gunting, pinset, dan needle holder) sebelum dan sesudah operasi. Sampel kemudian dianalisis melalui isolasi, pewarnaan Gram, dan uji biokimia untuk identifikasi bakteri. Hasil penelitian menunjukkan pertumbuhan bakteri pada peralatan medis sebelum dan sesudah operasi. Beberapa spesies bakteri teridentifikasi, termasuk Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Klebsiella sp., dan Bacillus subtilis. Peningkatan kontaminasi bakteri setelah operasi menggarisbawahi perlunya sterilisasi yang lebih efektif untuk mencegah infeksi nosokomial. Penelitian ini menyoroti risiko kontaminasi bakteri di ruang operasi dan pentingnya protokol sterilisasi yang lebih ketat demi keselamatan pasien.
Sosialisasi Pembelajaran Berbasis Teknologi untuk Orang Tua dalam Mendukung Pendidikan Anak di Era Digital Yusriani Mangarengi; Dipa Teruna Awaludin; Dewi Mayangsari; Indah Putri Santri; Rabiyatul Adawiyah
JIPITI: Jurnal Pengabdian kepada Masyarakat Vol. 2 No. 1 (2025): Februari 2025 - JIPITI: Jurnal Pengabdian kepada Masyarakat
Publisher : PT. Technology Laboratories Indonesia (TechnoLabs)

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Abstract

Di era digital saat ini, teknologi telah menjadi bagian integral dari kehidupan sehari-hari, termasuk dalam bidang pendidikan. Namun, tidak semua orang tua memiliki pemahaman yang memadai tentang bagaimana memanfaatkan teknologi untuk mendukung pendidikan anak-anak mereka. Program sosialisasi yang dirancang ini untuk meningkatkan literasi teknologi di kalangan orang tua, dengan tujuan membantu mereka dalam mendukung pembelajaran anak-anak di rumah. Program ini melibatkan serangkaian workshop dan sesi pelatihan yang berfokus pada penggunaan perangkat digital, aplikasi pendidikan, dan strategi pengawasan penggunaan teknologi oleh anak. Hasil dari kegiatan ini menunjukkan peningkatan signifikan dalam pemahaman dan keterampilan orang tua dalam memanfaatkan teknologi secara efektif. Dengan demikian, diharapkan orang tua dapat berperan lebih aktif dalam proses pendidikan anak, serta mampu menciptakan lingkungan belajar yang kondusif di rumah. Program ini juga menekankan pentingnya kolaborasi antara sekolah dan orang tua dalam menghadapi tantangan pendidikan di era digital
Literature Review : Isolation and Identification of Bacteria in Children with Respiratory Infections Wardatul Jannah; Yusriani Mangarengi; Nurhikmawati; Yani Sodiqah; Marzelina Karim
International Journal of Health Engineering and Technology Vol. 4 No. 4 (2025): IJHET NOVEMBER 2025
Publisher : CV. AFDIFAL MAJU BERKAH

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Abstract

Background: Acute Respiratory Tract Infection (ARI) is one of the most common diseases and a leading cause of death in toddlers in Indonesia. This disease can be caused by bacteria, viruses, fungi, or aspiration. Objective: This study aims to determine the types of bacteria present in children with ARI and the most common bacteria found in children with ARI. Method: The study used a literature review with a narrative review design, where the research process started from searching in database search engines: Pubmed, NCBI, Google Scholar, then sorted related to the title of Isolation and Identification of ISPA bacteria in children in 2017-2025, The search results yielded 904 journals. The journals were re-selected based on the standard isolation method and PCR (Polymerase Chain Reaction) with keywords acute respiratory tract infection, ISPA children, ISPA bacteria children, yielding 60 journals. The next selection looked at the entire text containing tables and graphs, yielding 32 journals. Then, they were re-selected by looking at their suitability in the field of microbiology, yielding 8 journals. Results: The results of the study showed that various types of bacteria were successfully identified as the cause of ARI in children, including Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Corynebacterium diphtheriae, Moraxella catarrhalis, Bacillus sp., and Sphingomonas paucimobilis. Most studies reported that Gram-positive bacteria were more dominant, with Streptococcus pneumoniae and Staphylococcus aureus as the most common species, although Gram-negative bacteria such as Klebsiella pneumoniae and Pseudomonas aeruginosa were also found, especially in cases of severe ARI. Conclusion: This study shows that acute respiratory infections (ARI) in children are caused by various bacteria, predominantly Gram-positive. Streptococcus pneumoniae and Staphylococcus aureus are the most frequently found bacteria, while Klebsiella pneumoniae and Pseudomonas aeruginosa play a significant role in more severe cases.