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Silvita
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Potensi Bakteri Gram Positif dalam Eco-Enzym dari Tumbuhan Lahan Gambut: Inovasi Sumber Belajar Bioteknologi untuk Mengembangkan Kemampuan Penalaran Ilmiah Araina, Elga; Silvita; Uda, Saritha Kittie; Andani, Melda
Jurnal Penelitian Pendidikan IPA Vol 11 No 10 (2025): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i10.12923

Abstract

This study aims to observe Gram-positive bacteria in eco-enzymes produced from the fermentation of peatland plants in Central Kalimantan and to analyze their potential as biotechnology learning resources for developing students’ scientific explanation skills. The research employed a descriptive qualitative method through eco-enzyme preparation, bacterial identification using Gram staining, and analysis of the role of observational results in the learning context. The findings revealed four Gram-positive bacteria, namely Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, and Lactobacillus bulgaricus. The presence of these bacteria demonstrates the active involvement of microorganisms in the fermentation process, which can serve as a concrete example for students to understand biotechnology concepts. These results emphasize that bacterial identification outcomes are not only scientifically valuable but also hold potential as contextual learning resources that support the development of scientific explanation. Through these observational results, students can practice constructing claims, presenting evidence, and connecting them with scientific reasoning. Therefore, this study concludes that exploring Gram-positive bacteria in eco-enzymes derived from peatland plants has the potential to enrich biotechnology learning while simultaneously developing students’ scientific explanation skills.
From Culture to Science: Exploring Scientific Knowledge in the Making of Wadi, a Traditional Dish of the Dayak and Banjar Silvita; Prasetyo, Opik; Ruwaida, Ulya; Aurora, Zevira Fransisca; Oktaviana, Rina
Jurnal Penelitian Pendidikan IPA Vol 11 No 12 (2025): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i12.12413

Abstract

Traditional food processing practices often contain implicit scientific knowledge that can be systematically analyzed and explained using modern science. This study examines the scientific knowledge embedded in the traditional preparation of Wadi, a fermented fish product of the Dayak and Banjar communities. The research aims to identify and describe forms of scientific knowledge represented in each stage of the Wadi-making process. This study employed a qualitative descriptive method using documentation analysis. Data were collected from selected written and visual sources that clearly describe the stages of Wadi production. The data were analyzed by mapping traditional practices to scientific concepts in food science. The results indicate that the preparation of Wadi embodies multiple forms of scientific knowledge, including osmosis in the salting process, salt-based preservation through microbial inhibition, lactic acid fermentation, moisture control, and biochemical transformations such as protein hydrolysis. These findings demonstrate that traditional culinary practices are not merely cultural activities but also reflect systematic scientific knowledge consistent with principles of microbiology, biochemistry, and food preservation. In conclusion, the scientific knowledge identified in the making of Wadi has strong potential to support contextual and culturally relevant science learning by connecting everyday practices with formal scientific concepts.
Optimization of in Vitro Culture of Taro Banana (Musa paradisiaca var. sapientum L.) Based on Thidiazuron: A Foundation for Developing a Biotechnology Booklet to Enhance Decision-Making Skills Purnaningsih, Titin; Kimeni; Gunawan, Yohannes Edi; Akhmadi; Nuriman; Adam, Chaidir; Silvita; Fahrina, Ririn; Rahmi, Ennike Gusti
Jurnal Penelitian Pendidikan IPA Vol 11 No 12 (2025): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i12.13368

Abstract

This study aims to optimize the concentration of Thidiazuron (TDZ) in the in vitro culture of taro banana (Musa paradisiaca var. sapientum L.) and to integrate the results as a foundation for developing a biotechnology booklet to enhance decision-making skills. The research was conducted using an experimental method with various TDZ concentration treatments, observing parameters such as the number of shoots and leaves produced. To bridge the gap between biotechnological research and its application in education, this study investigates the optimization of Thidiazuron (TDZ) concentrations for the in vitro multiplication of taro banana (Musa paradisiaca var. sapientum L.). The results showed that the use of TDZ effectively induced shoot formation, with the optimal concentration obtained at 0.01 mg/L TDZ, producing an average of 22.25 shoots and 24.25 leaves. In addition to generating biological data, each stage of this study reflected the implementation of decision-making skills, including problem identification, information gathering, criteria determination, solution selection, and result evaluation. These findings serve as the foundation for developing a biotechnology booklet as a contextual learning medium that not only facilitates the understanding of biotechnology concepts but also trains students to make scientific and reflective decisions.
Co-Authors Adam, Chaidir Akhmadi Andani, Melda Araina, Elga Aurora, Zevira Fransisca Ennike Gusti Rahmi Fahrina, Ririn Gunawan, Yohannes Edi Kimeni Nuriman Prasetyo, Opik Rina Oktaviana RUWAIDA, ULYA Titin Purnaningsih Uda, Saritha Kittie