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Evaluasi Kultivar Apel dan Zat Pengatur Tumbuh (Sitokinin-Auksin) Terhadap Tingkat Keberhasilan Eksplan Apel Secara In Vitro: Evaluation of Apple Cultivars and Growth Regulators (Cytokinin-Auxin) on The Success Rate of Apple Explants In Vitro Hidayatullah, Careca Sepdihan Rahmat; Santoso, Untung; Nursandi, Fatimah; Simanjuntak, Rich Gemilang; Priliraningrum, Oentari
Agrocentrum Vol. 2 No. 2 (2024): Agrocentrum
Publisher : Agriculture Faculty - UPN "Veteran Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/agrocentrum.v2i2.45

Successful in vitro propagation can support the provision of large, fast and highquality apple seeds. Growth regulators commonly used in culture techniques are cytokinins and auxins. the addition of 4 ppm BAP to the culture media can increase the lipid content and biomass 1.26 times higher in microalgae. This study aims to evaluate the ability of apple cultivars and the growth regulators BAP (Benzyl amino purine) and IAA (Indole acetic acid) to regenerate apple shoots in vitro. The experimental design used RAK (randomized block design) 2 factors. The first factor was shoots from four apple cultivars, namely Fuji, Red delicious, gala, and manalagi. The media used were 1) MS + 3 mg/L BAP + 0.2 mg/L IAA, 2) MS + 6 mg/L BAP + 0.2 mg/L IAA, 3) MS + 3 mg/L BAP + 0.3 mg/L IAA, and 4) MS + 6 mg/L BAP + 0.3 mg/L IAA. Based on the percentage of live explants, apple shoots show a relatively high level of success in the Fuji apple cultivar for all BAP-IAA growth regulator compositions, namely 3 mg/L BAP + 0.2 mg/L IAA, 6 mg/L BAP + 0.2 mg/L IAA, 3 mg/L BAP + 0.3 mg/L IAA, and 6 mg/L BAP + 0.3 mg/L IAA. Apple cultivars and growth regulators did not show a significant effect on the percentage of live explants, green color of shoots, explant contamination and number of leaves. There is a need for further studies regarding the type of explant with the right type of cytokinin hormone to regenerate green shoots more optimally.

STUDI KANDUNGAN CAPSAISIN CABAI RAWIT (Capsicum frutescens L.) AKIBAT PEMBERIAN MUTAGEN KIMIA EMS (ETHYL METHANE SULFONATE) Arrufitasari, Putri Nur; Fikri, Galih El; Handini, Aline Sisi; Sutanto, Oentari Prilaningrum; Hidayatullah, Careca Sepdihan Rahmat
AGRICA Vol. 18 No. 1 (2025): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v18i1.5678

Chili pepper (Capsicum frutescens L.) is an important horticultural commodity in Indonesia with high economic value, primarily due to its capsaicin content, which provides its distinctive pungency and health benefits. This study aimed to evaluate the effect of Ethyl Methane Sulfonate (EMS) as a chemical mutagen agent on capsaicin content in the leaves and stems of three local chili pepper genotypes. The research was conducted using a two-factor Completely Randomized Design (CRD) with three replications, consisting of EMS concentrations (0.00%, 0.01%, 0.02%, and 0.04%) and three chili pepper genotypes. Mutation treatment was carried out by soaking seeds in EMS solution for six hours. Capsaicin content was analyzed using spectrophotometry at a wavelength of 280 nm. The results showed that EMS treatment had no significant effect on capsaicin content in either leaves or stems across all genotypes. However, there was a tendency for increased capsaicin content in certain treatment combinations, particularly at EMS concentrations of 0.01%. This suggests that EMS treatment at specific concentrations may induce physiological changes associated with capsaicin production.

STUDI KANDUNGAN CAPSAISIN CABAI RAWIT (Capsicum frutescens L.) AKIBAT PEMBERIAN MUTAGEN KIMIA EMS (ETHYL METHANE SULFONATE) Arrufitasari, Putri Nur; Fikri, Galih El; Handini, Aline Sisi; Sutanto, Oentari Prilaningrum; Hidayatullah, Careca Sepdihan Rahmat
AGRICA Vol. 18 No. 1 (2025): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v18i1.5678

Chili pepper (Capsicum frutescens L.) is an important horticultural commodity in Indonesia with high economic value, primarily due to its capsaicin content, which provides its distinctive pungency and health benefits. This study aimed to evaluate the effect of Ethyl Methane Sulfonate (EMS) as a chemical mutagen agent on capsaicin content in the leaves and stems of three local chili pepper genotypes. The research was conducted using a two-factor Completely Randomized Design (CRD) with three replications, consisting of EMS concentrations (0.00%, 0.01%, 0.02%, and 0.04%) and three chili pepper genotypes. Mutation treatment was carried out by soaking seeds in EMS solution for six hours. Capsaicin content was analyzed using spectrophotometry at a wavelength of 280 nm. The results showed that EMS treatment had no significant effect on capsaicin content in either leaves or stems across all genotypes. However, there was a tendency for increased capsaicin content in certain treatment combinations, particularly at EMS concentrations of 0.01%. This suggests that EMS treatment at specific concentrations may induce physiological changes associated with capsaicin production.

HIDROPONIK SOLUSI PERTANIAN MODERN TERKINI DI DESA KETINDAN RT 4 KABUPATEN MALANG Sutanto, Oentari Prilaningrum; Arrufitasari, Putri Nur; Hidayatullah, Careca Sepdihan Rahmat; Handini, Aline Sisi
Jurnal Edukasi Pengabdian Masyarakat Vol 4 No 2 (2025): APRIL 2025
Publisher : FIP UNIRA MALANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36636/eduabdimas.v4i2.6583

Desa Ketindan berada di wilayah Kecamatan Lawang Kabupaten Malang dengan ketinggian rata-rata 600 mdpl. Desa Ketindan terdapat didaerah yang sudah padat penduduk menyebabkan keterbatasan ruang lingkup untuk bertanam. Hal ini menjadi fokus untuk pengabdian masyarakat dalam memanfaatkan lahan terbatas menjadi produktif salah satunya adalah hidroponik. Sistem hidroponik memiliki banyak keunggulan dengan sistem yang sederhana, potensi ini memberikan harapan untuk masyarakat Desa Ketindan RT 04 dapat mengimplementasikanya. Penerapan hidroponik ini dapat menyalurkan hobi masyarakat perkotaan dalam bercocok tanam dilahan sempit, meningkatkan keasrian dan keindahan serta menjadi peluang usaha sampingan. Metode pelaksanaan pengabdian adalah pemaparan materi, persiapan dan perakitan sistem hidroponik secara sederhana. Hasil yang didapatkan dalam pengabdian masyarakat ini adalah penyuluhan tentang hidroponik didaerah perkotaan sehingga dapat meningkatkan pengetahuan dan keterampilan masyarakat dalam memanfaatkan hidroponik untuk mendukung kesejahteraan.

Efektivitas Berbagai Konsentrasi AB Mix terhadap Pertumbuhan dan Anatomi Tanaman Jagung (Zea mays L.) dalam Sistem Hidroponik Hidayatullah, Careca Sepdihan Rahmat; Priambodo, Okta Nindita; Sutanto, Oentari Prilaningrum; Arrufitasari, Putri Nur; Handini, Aline Sisi
Jurnal Agroekoteknologi Tropika Lembab Vol 8, No 1 (2025): Agroekoteknologi Tropika Lembab Volume 8 Nomor 1 Agustus 2025
Publisher : Mulawarman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jatl.8.1.2025.20367.70-76

Tanaman jagung (Zea mays L.) merupakan serealia terbesar dalam persebaran dan produksi yang berguna untuk sumber nutrisi manusia, pakan ternak, dan biofuel di dunia. Upaya peningkatan produksi dan ketahanan pangan jagung terus ditingkatkan dengan cara pemberian pupuk AB Mix yang tepat jenis dan dosis. Selain penggunaan pupuk AB Mix, perlu adanya inovasi dalam hal budidaya yaitu hidroponik sistem apung yang efisien. Penelitian bertujuan untuk mengetahui pengaruh berbagai konsentrasi AB Mix terhadap pertumbuhan dan anatomi tanaman jagung (Zea mays L.) pada sistem hidroponik. Percobaan telah dilakukan di dalam greenhouse di di Kebun Cikabayan Bawah, Institut Pertanian Bogor, Bogor, Indonesia dengan ketinggian tempat 234 m dpl. Rancangan percobaan menggunakan Rancangan Acak Kelompok Lengkap Teracak (RAKLT) sederhana dengan satu faktor, yaitu konsentrasi AB Mix. Perlakuan terdiri atas tiga taraf konsentrasi, yaitu ½ kali, 1 kali, dan 2 kali konsentrasi AB Mix. Masing-masing perlakuan diulang sebanyak enam kali, sehingga total terdapat 18 unit percobaan. Hasil analisis menunjukkan 2 kali konsentrasi AB Mix menunjukkan peningkatan yang siginfikan sebesar 107% dibandingkan dengan ½ kali konsentrasi AB Mix umur 4 minggu setelah semai (MSS). Tanaman jagung mengalami pertumbuhan dan hasil dengan baik pada perlakuan 2 kali konsentrasi AB Mix seperti ditunjukkan oleh parameter pengamatan tinggi tanaman, bobot basah tajuk umur 4MSS,bobotbasah akar 3-4MSS,danbobot basahtotal4MSS.Perlakuan 2 kali konsentrasi AB Mix adalah perlakuan terbaik dalam budidaya tanaman jagung (Zea mays L.) secara hidroponik hingga umur 4 MSS. Perlu adanya penelitian lebih lanjut terkait pengaruh konsentrasi AB Mix dengan berbagai varietas tanaman jagung dari aspek fisiologi hingga bobot panen jagung.

ANALISIS MUTU TOMAT (Solanum lycopersicum) BERDASARKAN CIRI FISIK DAN KANDUNGAN BIOKIMIA SELAMA PERIODE PENYIMPANAN: ANALYSIS OF TOMATO (Solanum lycopersicum) QUALITY BASED ON PHYSICAL CHARACTERISTICS AND BIOCHEMICAL CONTENT DURING STORAGE PERIOD Sutanto, Oentari Prilaningrum; Sepdihan Rahmat Hidayatullah, Careca; Nur Arrufitasari, Putri; Sisi Handini, Aline
AgriPeat Vol. 26 No. 02 (2025): JURNAL AGRIPEAT Vol. 26 No. 02 September 2025
Publisher : Fakultas Pertanian Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36873/agp.v26i02.22181

Tomat (Solanum lycopersicum) merupakan jenis produk hortikultura yang rentan mengalami kerusakan. Tomat termasuk juga buah klimaterik yang akan meningkatkan laju respirasi ketika memasuki fase pematangan karena produksi gas etilen yang akan meningkat. Proses pematangan yang cepat ini akan memberikan pengaruh terhadap metabolisme buah, umur simpan tomat menjadi pendek, dan menurunkan kualitas buah tomat. Untuk mengurangi kerusakan pada buah buah tomat diperlukan kegiatan pascapanen yang tepat untuk menjaga kualitas dari produk hortikultura salah satunya adalah suhu penyimpanan buah tomat. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 5 kali pengulangan. Perlakuan penyimpanan tomat pada suhu ruang (28-29oC) dan suhu rendah (10-12 oC) yang disimpan selama 12 hari. Penelitian ini bertujuan unuk mengevaluasi pengaruh suhu penyimpanan tomat. Hal ini dilakukan untuk mengetahui mutu dan kualitas tomat pada suhu penyimpanan yang berbeda baik pada uji organoleptik pada karakter fisik maupun karakter fisiologi buah tomat. Hasil penelitian menunjukkan bahwa suhu penyimpanan rendah dapat menekan pecah warna tomat, menjaga kadar vitamin C dan kadar gula, sedangkan suhu ruang meningkatkan pecah warna tomat skala 5 (merah) dan peningkatan kadar asam buah.

Respon Genotipe Talas Colocasia esculenta var esculenta dan var antiquorum pada Interval Pemberian Air Berbeda Hidayatullah, Careca Sepdihan Rahmat; Sopandie, Didy; Santosa, Edi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 48 No. 3 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Talas dikenal sebagai tanaman yang adaptif terhadap tanah dengan kelembaban tinggi. Dalam rangka mitigasi dampak perubahan iklim, respon genotipe tanaman talas terhadap variasi ketersediaan air perlu diketahui. Penelitian bertujuan mengevaluasi pengaruh frekuensi pemberian air pada pertumbuhan dan hasil tanaman talas tipe eddoe dan dasheen. Penelitian dilaksanakan di rumah kaca Kebun Percobaan Cikabayan IPB pada bulan Januari sampai April 2020. Sepuluh genotipe talas dievaluasi yang terdiri atas dua tipe dasheen (Talas Sutra dan Talas Bentul) dan delapan tipe eddoe (S6, S20, S24, S26, S28, S34, S35, dan S36). Tanaman umur delapan minggu dalam polibag diberi air dengan frekuensi berbeda yakni 1, 3, 7, dan 15 hari sekali hingga jenuh air. Hasil penelitian menunjukkan pertumbuhan semua tipe talas lebih vigor pada interval pemberian air 1 dan 3 hari sekali seperti ditunjukkan oleh peubah tinggi, jumlah daun, jumlah anakan, dan diameter petiol. Bobot basah umbi semua tipe talas mencapai 2-3 kali lipat pada perlakuan interval pemberian air 1 dan 3 hari dibandingkan dengan interval 7 dan 15 hari sekali; maka talas termasuk sensitif terhadap rendahnya kelembaban tanah. Kata kunci: cekaman kekeringan, perubahan iklim, satoimo, talas dasheen, talas eddoe

KARAKTERISTIK PERTUMBUHAN FISIOLOGIS DAN ANATOMI TANAMAN KEDELAI (GLYCINE MAX L.) TERHADAP TINGKAT KONSENTRASI AB MIX PADA SISTEM HIDROPONIK Careca Sepdihan Rahmat Hidayatullah
Agrica Ekstensia Vol 19 No 2 (2025): Edisi Desember
Publisher : Politeknik Pembangunan Pertanian Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55127/ae.v19i2.259

Soybean plants (Glycine max L.) are among the primary sources of vegetable oil and require essential nutrients to support their growth and yield. In order to enhance the morphological quality and seed production of soybean such as plant height, stem diameter, number of filled pods, and number of seeds per plant alternative developments in hydroponic technology are needed. This study aimed to evaluate the effects of different concentrations of AB Mix nutrient solution on the growth, physiological, and anatomical characteristics of soybean plants in a hydroponic system. The experiment was conducted in a greenhouse at the Lower Cikabayan Garden, Bogor Agricultural University, Indonesia, using a simple Randomized Complete Block Design (RCBD). The treatments consisted of three AB Mix concentration levels: 1/2x, 1x, and 2x the standard concentration. The 1x AB Mix concentration treatment (10 mL L⁻¹) consistently resulted in relatively higher plant height and number of leaves—by 7.9% and 0.9%, respectively—compared to the 1/2x and 2x treatments. However, the differences were not statistically significant. The study concluded that varying AB Mix concentrations (1/2x, 1x, and 2x) did not significantly affect the growth, physiology, or anatomy of soybean plants in a hydroponic system. Further research is recommended to explore the interaction between AB Mix concentrations and various soybean genotypes to achieve optimal production

Biomass and Chlorophyll Responses of Corn Plants (Zea mays L.) to Varying Abmix Concentrations in A Hydroponic Environment Careca Sepdihan Rahmat Hidayatullah; Okta Nindita Priambodo; Oentari Prilaningrum Sutanto; Bagus Arrasyid
International Journal of Agriculture System VOLUME 13 ISSUE 2, DECEMBER 2025
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v13i2.6603

Corn (Zea mays L.) is the second most important agricultural commodity in Indonesia, after rice. Corn plays an important role as a raw material for animal feed, bioenergy (biofuel), and various other industrial products. However, corn productivity is highly dependent on the availability of nutrients in the growing medium. Therefore, this study aims to evaluate the effect of various AB Mix concentrations on the biomass and chlorophyll of corn (Zea mays L.) plants in a hydroponic system. The experimental design used a simple randomized complete block design (RCBD) with one factor, namely the AB Mix concentration (1/2x, 1x, and 2x) and correlation analysis using R-Studio version 2025. At 6 WAP, differences in shoot, root, and total biomass between treatments were still significant. 2x AB Mix concentration again produced the highest biomass, namely 29.67 g. Variations in AB Mix concentration have a significant effect on biomass yield (biomass of shoots at 5-6 WAP, biomass of roots at 6 WAP, and total weight at 5-6 WAP) and chlorophyll a, b content, chlorophyll a+b ratio in corn leaves.

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