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Evaluasi Kultivar Apel dan Zat Pengatur Tumbuh (Sitokinin-Auksin) Terhadap Tingkat Keberhasilan Eksplan Apel Secara In Vitro: Evaluation of Apple Cultivars and Growth Regulators (Cytokinin-Auxin) on The Success Rate of Apple Explants In Vitro Hidayatullah, Careca Sepdihan Rahmat; Santoso, Untung; Nursandi, Fatimah; Simanjuntak, Rich Gemilang; Priliraningrum, Oentari
Agrocentrum Vol. 2 No. 2 (2024): Agrocentrum
Publisher : Agriculture Faculty - UPN "Veteran Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/agrocentrum.v2i2.45

Successful in vitro propagation can support the provision of large, fast and highquality apple seeds. Growth regulators commonly used in culture techniques are cytokinins and auxins. the addition of 4 ppm BAP to the culture media can increase the lipid content and biomass 1.26 times higher in microalgae. This study aims to evaluate the ability of apple cultivars and the growth regulators BAP (Benzyl amino purine) and IAA (Indole acetic acid) to regenerate apple shoots in vitro. The experimental design used RAK (randomized block design) 2 factors. The first factor was shoots from four apple cultivars, namely Fuji, Red delicious, gala, and manalagi. The media used were 1) MS + 3 mg/L BAP + 0.2 mg/L IAA, 2) MS + 6 mg/L BAP + 0.2 mg/L IAA, 3) MS + 3 mg/L BAP + 0.3 mg/L IAA, and 4) MS + 6 mg/L BAP + 0.3 mg/L IAA. Based on the percentage of live explants, apple shoots show a relatively high level of success in the Fuji apple cultivar for all BAP-IAA growth regulator compositions, namely 3 mg/L BAP + 0.2 mg/L IAA, 6 mg/L BAP + 0.2 mg/L IAA, 3 mg/L BAP + 0.3 mg/L IAA, and 6 mg/L BAP + 0.3 mg/L IAA. Apple cultivars and growth regulators did not show a significant effect on the percentage of live explants, green color of shoots, explant contamination and number of leaves. There is a need for further studies regarding the type of explant with the right type of cytokinin hormone to regenerate green shoots more optimally.

STUDI KANDUNGAN CAPSAISIN CABAI RAWIT (Capsicum frutescens L.) AKIBAT PEMBERIAN MUTAGEN KIMIA EMS (ETHYL METHANE SULFONATE) Arrufitasari, Putri Nur; Fikri, Galih El; Handini, Aline Sisi; Sutanto, Oentari Prilaningrum; Hidayatullah, Careca Sepdihan Rahmat
AGRICA Vol. 18 No. 1 (2025): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v18i1.5678

Chili pepper (Capsicum frutescens L.) is an important horticultural commodity in Indonesia with high economic value, primarily due to its capsaicin content, which provides its distinctive pungency and health benefits. This study aimed to evaluate the effect of Ethyl Methane Sulfonate (EMS) as a chemical mutagen agent on capsaicin content in the leaves and stems of three local chili pepper genotypes. The research was conducted using a two-factor Completely Randomized Design (CRD) with three replications, consisting of EMS concentrations (0.00%, 0.01%, 0.02%, and 0.04%) and three chili pepper genotypes. Mutation treatment was carried out by soaking seeds in EMS solution for six hours. Capsaicin content was analyzed using spectrophotometry at a wavelength of 280 nm. The results showed that EMS treatment had no significant effect on capsaicin content in either leaves or stems across all genotypes. However, there was a tendency for increased capsaicin content in certain treatment combinations, particularly at EMS concentrations of 0.01%. This suggests that EMS treatment at specific concentrations may induce physiological changes associated with capsaicin production.

STUDI KANDUNGAN CAPSAISIN CABAI RAWIT (Capsicum frutescens L.) AKIBAT PEMBERIAN MUTAGEN KIMIA EMS (ETHYL METHANE SULFONATE) Arrufitasari, Putri Nur; Fikri, Galih El; Handini, Aline Sisi; Sutanto, Oentari Prilaningrum; Hidayatullah, Careca Sepdihan Rahmat
AGRICA Vol. 18 No. 1 (2025): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v18i1.5678

Chili pepper (Capsicum frutescens L.) is an important horticultural commodity in Indonesia with high economic value, primarily due to its capsaicin content, which provides its distinctive pungency and health benefits. This study aimed to evaluate the effect of Ethyl Methane Sulfonate (EMS) as a chemical mutagen agent on capsaicin content in the leaves and stems of three local chili pepper genotypes. The research was conducted using a two-factor Completely Randomized Design (CRD) with three replications, consisting of EMS concentrations (0.00%, 0.01%, 0.02%, and 0.04%) and three chili pepper genotypes. Mutation treatment was carried out by soaking seeds in EMS solution for six hours. Capsaicin content was analyzed using spectrophotometry at a wavelength of 280 nm. The results showed that EMS treatment had no significant effect on capsaicin content in either leaves or stems across all genotypes. However, there was a tendency for increased capsaicin content in certain treatment combinations, particularly at EMS concentrations of 0.01%. This suggests that EMS treatment at specific concentrations may induce physiological changes associated with capsaicin production.

HIDROPONIK SOLUSI PERTANIAN MODERN TERKINI DI DESA KETINDAN RT 4 KABUPATEN MALANG Sutanto, Oentari Prilaningrum; Arrufitasari, Putri Nur; Hidayatullah, Careca Sepdihan Rahmat; Handini, Aline Sisi
Jurnal Edukasi Pengabdian Masyarakat Vol 4 No 2 (2025): APRIL 2025
Publisher : FIP UNIRA MALANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36636/eduabdimas.v4i2.6583

Desa Ketindan berada di wilayah Kecamatan Lawang Kabupaten Malang dengan ketinggian rata-rata 600 mdpl. Desa Ketindan terdapat didaerah yang sudah padat penduduk menyebabkan keterbatasan ruang lingkup untuk bertanam. Hal ini menjadi fokus untuk pengabdian masyarakat dalam memanfaatkan lahan terbatas menjadi produktif salah satunya adalah hidroponik. Sistem hidroponik memiliki banyak keunggulan dengan sistem yang sederhana, potensi ini memberikan harapan untuk masyarakat Desa Ketindan RT 04 dapat mengimplementasikanya. Penerapan hidroponik ini dapat menyalurkan hobi masyarakat perkotaan dalam bercocok tanam dilahan sempit, meningkatkan keasrian dan keindahan serta menjadi peluang usaha sampingan. Metode pelaksanaan pengabdian adalah pemaparan materi, persiapan dan perakitan sistem hidroponik secara sederhana. Hasil yang didapatkan dalam pengabdian masyarakat ini adalah penyuluhan tentang hidroponik didaerah perkotaan sehingga dapat meningkatkan pengetahuan dan keterampilan masyarakat dalam memanfaatkan hidroponik untuk mendukung kesejahteraan.

Effectiveness of Various AB Mix Concentrations on the Growth and Anatomy of Corn Plants (Zea mays L) in Hydroponic Systems Hidayatullah, Careca Sepdihan Rahmat; Priambodo, Okta Nindita; Sutanto, Oentari Prilaningrum; Arrufitasari, Putri Nur; Handini, Aline Sisi
Jurnal Agroekoteknologi Tropika Lembab Vol 8, No 1 (2025): Agroekoteknologi Tropika Lembab Volume 8 Nomor 1 Agustus 2025
Publisher : Mulawarman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jatl.8.1.2025.20367.70-76

Corn (Zea mays L.) is the most widely cultivated cereal crop globally, serving as a vital source of food, animal feed, and biofuel. Efforts to improve corn production and ensure food security continue through advancements in fertilization and cultivation techniques. This study investigates the effect of different concentrations of AB Mix fertilizer on the growth and anatomical development of corn grown using an efficient floating hydroponic system. The experiment was conducted in a greenhouse at the Cikabayan Bawah Garden, Bogor Agricultural University, Bogor, Indonesia, located at an altitude of 234 meters above sea level. A completely randomized design (CRD) was used, with one factor—AB Mix concentration—at three levels: ½×, 1×, and 2× the standard concentration. Each treatment was replicated six times, resulting in 18 experimental units. Results showed that the 2× AB Mix concentration significantly enhanced plant performance, with a 107% increase in growth compared to the ½× concentration at 4 weeks after sowing (WAS). Parameters such as plant height, shoot fresh weight at 4 WAS, root fresh weight at 3–4 WAS, and total fresh weight at 4 WAS all showed marked improvements with the 2× treatment. These findings indicate that doubling the standard AB Mix concentration is optimal for hydroponic corn cultivation up to 4 WAS. Further studies are recommended to assess the physiological responses and yield outcomes across different corn varieties under varying AB Mix concentrations

ANALISIS MUTU TOMAT (Solanum lycopersicum) BERDASARKAN CIRI FISIK DAN KANDUNGAN BIOKIMIA SELAMA PERIODE PENYIMPANAN: ANALYSIS OF TOMATO (Solanum lycopersicum) QUALITY BASED ON PHYSICAL CHARACTERISTICS AND BIOCHEMICAL CONTENT DURING STORAGE PERIOD Sutanto, Oentari Prilaningrum; Sepdihan Rahmat Hidayatullah, Careca; Nur Arrufitasari, Putri; Sisi Handini, Aline
AgriPeat Vol. 26 No. 02 (2025): JURNAL AGRIPEAT Vol. 26 No. 02 September 2025
Publisher : Fakultas Pertanian Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36873/agp.v26i02.22181

Tomat (Solanum lycopersicum) merupakan jenis produk hortikultura yang rentan mengalami kerusakan. Tomat termasuk juga buah klimaterik yang akan meningkatkan laju respirasi ketika memasuki fase pematangan karena produksi gas etilen yang akan meningkat. Proses pematangan yang cepat ini akan memberikan pengaruh terhadap metabolisme buah, umur simpan tomat menjadi pendek, dan menurunkan kualitas buah tomat. Untuk mengurangi kerusakan pada buah buah tomat diperlukan kegiatan pascapanen yang tepat untuk menjaga kualitas dari produk hortikultura salah satunya adalah suhu penyimpanan buah tomat. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 5 kali pengulangan. Perlakuan penyimpanan tomat pada suhu ruang (28-29oC) dan suhu rendah (10-12 oC) yang disimpan selama 12 hari. Penelitian ini bertujuan unuk mengevaluasi pengaruh suhu penyimpanan tomat. Hal ini dilakukan untuk mengetahui mutu dan kualitas tomat pada suhu penyimpanan yang berbeda baik pada uji organoleptik pada karakter fisik maupun karakter fisiologi buah tomat. Hasil penelitian menunjukkan bahwa suhu penyimpanan rendah dapat menekan pecah warna tomat, menjaga kadar vitamin C dan kadar gula, sedangkan suhu ruang meningkatkan pecah warna tomat skala 5 (merah) dan peningkatan kadar asam buah.

Respon Genotipe Talas Colocasia esculenta var esculenta dan var antiquorum pada Interval Pemberian Air Berbeda Hidayatullah, Careca Sepdihan Rahmat; Sopandie, Didy; Santosa, Edi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 48 No. 3 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Talas dikenal sebagai tanaman yang adaptif terhadap tanah dengan kelembaban tinggi. Dalam rangka mitigasi dampak perubahan iklim, respon genotipe tanaman talas terhadap variasi ketersediaan air perlu diketahui. Penelitian bertujuan mengevaluasi pengaruh frekuensi pemberian air pada pertumbuhan dan hasil tanaman talas tipe eddoe dan dasheen. Penelitian dilaksanakan di rumah kaca Kebun Percobaan Cikabayan IPB pada bulan Januari sampai April 2020. Sepuluh genotipe talas dievaluasi yang terdiri atas dua tipe dasheen (Talas Sutra dan Talas Bentul) dan delapan tipe eddoe (S6, S20, S24, S26, S28, S34, S35, dan S36). Tanaman umur delapan minggu dalam polibag diberi air dengan frekuensi berbeda yakni 1, 3, 7, dan 15 hari sekali hingga jenuh air. Hasil penelitian menunjukkan pertumbuhan semua tipe talas lebih vigor pada interval pemberian air 1 dan 3 hari sekali seperti ditunjukkan oleh peubah tinggi, jumlah daun, jumlah anakan, dan diameter petiol. Bobot basah umbi semua tipe talas mencapai 2-3 kali lipat pada perlakuan interval pemberian air 1 dan 3 hari dibandingkan dengan interval 7 dan 15 hari sekali; maka talas termasuk sensitif terhadap rendahnya kelembaban tanah. Kata kunci: cekaman kekeringan, perubahan iklim, satoimo, talas dasheen, talas eddoe

Shoot multiplication growth of some apple cultivars with a combination of auxin and cytokinin hormones in vitro Santoso, Untung; Nursandi, Fatimah; Hidayatullah, Careca Sepdihan Rahmat
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 3 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i3.59894

Apples (Malus sylvestris) have been grown in Indonesia, especially in highland areas such as Batu, Nongkojajar, and Poncokusumo. In order to propagate apple plants through in vitro, organ culture is performed in a controlled environment, free of pests and diseases. The research aimed to evaluate the effect of the composition of the growth regulator BAP-IAA on the growth and shoot multiplication of several apple cultivars in vitro. The research was carried out at the Indonesian Orchid Partners Laboratory Jl. Hasanudin 1 No 24 Junrejo District, Batu City, Indonesia. The four apple cultivars used were Fuji, Red Delicious, Gala, and Manalagi apples. Four types of growth regulator composition were the treatments: 3 mg L-1 BAP and 0.2 mg L-1 IAA, 6 mg L-1 BAP and 0.2 mg L-1 IAA, 3 mg L-1 BAP and 0.3 mg L-1 IAA, and 6 mg L-1 BAP and 0.3 mg L-1 IAA. The results showed that growth and yield were more precise in the combination of Red Delicious with the growth enhancer composition of 3 mg L-1 BAP and 0.3 mg L-1 IAA, as shown by the variable number of shoots and fresh weight of explants. The results showed that the combination of the Red Delicious apple cultivar with BAP 3 mg L-1+IAA 0.3 mg L-1 can be recommended as the best treatment in mass shoot production/multiplication. Keywords: composition; cytokinin-auxin; apple cultivars; shoot multiplication

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