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All Journal BioWallacea Journal of Biological Research Jurnal IPA Terpadu Jurnal Pendidikan dan Pembelajaran Sains Indonesia (JPPSI) Jurnal Pendidikan dan Pembelajaran Sains Indonesia (JPPSI) Jurnal Dedikasi Seminar Nasional Pengabdian Kepada Masyarakat INOVASI: Jurnal Hasil Pengabdian Masyarakat Prosiding Seminar Nasional Dies Natalis Universitas Negeri Makassar JKM: Jurnal Kemitraan Masyarakat

Haji Muhiddin, Nurhayani

Fakultas MIPA, Universitas Negeri Makassar

Author-ID : 856731

Agriculture, Biological Sciences & Forestry Humanities Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Veterinary Other

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Analisis Kuantitatif Mikroorganisme pada Ragi Tape Lokal dan Daya Terima Tape Jusinta yang dihasilkan Nurhayani H. Muhiddin; Ramlawati Ramlawati; Nur Arfa Yanti; Abdul Mun'im
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 6, No 2 (2019): BioWallacea and Biotechnological Science
Publisher : University of Halu Oleo

The purpose of this study were to analyze the quantity of microorganisms in some local ragi tape and determine the panelists' acceptance of the "tape jusinta" was produced. This type of research is an experiment with a solid substrate fermentation method. The used ragi tape is 4 kinds of local ragi tape from South Sulawesi Province (ragi tape A, B, C, and D) and 1 type of local ragi tape from Southeast Sulawesi Province (ragi tape E). The Tape Jusinta was produced by fermentation of a solid mixture of purple sweet potato and cassava tubers in a ratio of 1: 1 using 5 kinds of local ragi tape. Analysis of microbial quantity in ragi tape includes the number of molds, yeasts and bacteria in viable count using the Standard Plate Count (SPC) method. Panelist acceptance of the Tape Jusinta product was analyzed using the Hedonic test with a numerical scale range of "6". Data were analyzed descriptively and inferentially using Anova and Duncan's Multiple Test. The results of the data analysis showed that the level of palatability of the panelists to the tapejusinta fermentedragi tape B was not different from the tape jusinta fermented ragi tape A and C, but the tape jusinta fermented by Cragi tape was different from the results of fermentation ragi tape D and E. The “tape jusinta” fermented ragi tape B is preferred over the tape jusinta fermented ragi tape A, C, D and E with the highest average panelist rating of 4.15. The quantity of microorganisms on ragi tape B are mold types as much as 1.0 x 104 cfu / g, yeast 8.0 x 103 cfu / g and bacteria 5.7 x 104 cfu/ g. Keywords: cassava roots, purple sweet potatoes, ragi tape, "tape jusinta", palatabilityAbstrakTujuan penelitian adalah menganalisis kuantitas mikroorganisme pada beberapa ragi tape lokal dan mengetahui daya terima panelis terhadap “tape jusinta” yang dihasilkan. Jenis penelitian adalah eksperimen dengan metode fermentasi substrat padat. Ragi tape yang digunakan adalah 4 macam ragi tape lokal yang berasal dari Provinsi Sulawesi Selatan (ragi tape A, B, C, dan D) dan 1 macam berasal dari Provinsi Sulawesi Tenggara (ragi tape E). Tape Jusinta dihasilkan melalui fermentasi substrat padat campuran ubi jalar ungu dan umbi ubi kayu dengan perbandingan 1 : 1 menggunakan 5 macam ragi tape lokal. Analisis kuantitas mikroba pada ragi tape meliputi jumlah kapang, khamir dan bakteri secara viable countmenggunakan metode Standard Plate Count (SPC). Daya terima panelis terhadap produk Tape Jusinta dianalisis menggunakan uji Hedonik dengan rentang skala numerik “6”.Data dianalisis secara deskriptif dan inferensial dengan Anava dan Duncan’s Multiple Test. Hasil analisis data menunjukkan bahwa tingkat kesukaan (palatability) panelis terhadap tape jusinta hasil fermentasi ragi tape B tidak berbeda dengan tape jusinta hasil fermentasi ragi tape A dan C, namun tape jusinta hasil fermentasi ragi tape C berbeda dengan hasil fermentasi ragi tape D dan E. Tape jusinta hasil fermentasi ragi tape B lebih disukai daripada tape jusinta hasil fermentasi ragi tape A, C, D dan E dengan rata-rata penilaian panelis tertinggi yaitu 4.15. Kuantitas mikroorganisme pada ragi tape B yaitu jenis kapang sebanyak 1.0 x 104 cfu/g, khamir 8.0 x 103 cfu/g dan bakteri 5.7 x 104 cfu/g .Kata kunci: Umbi ubi kayu, ubi jalar ungu, ragi tape, “tape jusinta”, daya terima

Isolasi dan Seleksi Bakteri Pendegradasi Solar Dari Pelabuhan Penyeberangan Kendari – Wawonii, Sulawesi Tenggara M. Rajab Sutra Mijaya; Nur Arfa Yanti; Ardiansyah Ardiansyah; Nurhayani H. Muhiddin
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 6, No 2 (2019): BioWallacea and Biotechnological Science
Publisher : University of Halu Oleo

This study aimed to obtain the ability of bacteria to degrade diesel fuel. Method of this research was exploration method. Bacteria were isolated by enrichment method used SMSSe enriched diesel 2% (v/v). Selection of hidrocarbonoclastic bacteria based on the ability of to grow on agar medium solid. The bacteria in the test made in the form of suspension with Mc Farland Standard 0.5. Test of bacterial isolates were used 10% of the inoculum put in 150 mL media with different concentrations of diesel fuel were 1%, 2% and 3% and incubated on a rotary shaker at 120 rpm. Samples were taken on 1, 5, 10, 15 and 20 days to test diesel emulsion by centrifugation at a speed of 3500 rpm ±15 minutes, the comparison between the media and diesel 4:1. Growth in the amount of bacteria accounted by a Standard Plate Count method. The levels of the diesel rest calculated every sampling during incubation. The selection results of obtained diesel degrading bacteria isolates that PSI.1 PSII.1 and PSIII.2. All of bacteria have the ability to degrade diesel fuel in different treatment, the best result in lowered diesel fuel that were a concentration of 3% during 20 days of incubated at PSII.1 isolate, that have the highest ability to reduce levels of diesel up to 70,70%.Keywords: Hydrocarbonoclastic, Degradation, Diesel fuels ABSTRAK Penelitian ini bertujuan untuk mengetahui kemampuan bakteri dalam mendegradasi solar. Penelitian ini adalah penelitian eksploratif. Isolasi bakteri menggunakan metode enrichment dengan media yang digunakan yaitu SMSSe yang diperkaya solar 2% (v/v). Pemilihan bakteri hidrokarbonoklastik berdasarkan kemampuan bakteri tumbuh pada media agar padat. Inokulum bakteri dibuat dalam bentuk suspensi dengan Standar Mc Farland 0,5. Pengujian kemampuan degradasi solar dilakukan menggunakan media minimal dengan variasi konsentrasi solar 1%, 2% dan 3% dan diinkubasi pada rotary shaker. Sampel diambil pada hari ke 1, 5, 10, 15 dan 20 untuk uji emulsi solar dengan menghitung volume solar yang teremulsi. Pertumbuhan jumlah bakteri dihitung dengan metode Standard Plate Count. Kadar sisa solar dihitung setiap pengambilan sampel selama inkubasi. Hasil seleksi bakteri pendegradasi solar diperoleh tiga isolat yaitu PSI.1 PSII.1 dan PSIII.2. Ketiga isolat bakteri memiliki kemampuan mendegradasi solar pada perlakuan yang berbeda, hasil terbaik dalam menurunkan kadar solar yaitu konsentrasi 3% selama 20 hari inkubasi pada isolat PSII.1 yang memiliki kemampuan tertinggi menurunkan kadar solar hingga 70,70%. Kata kunci : Hidrokarbonoklastik, Pendegradasi, Solar

Co-Authors Abdul Mun'im Ahmad Syakur Ardiansyah Ardiansyah Arif, Rifda Nurhikmahwati Hasanuddin Hasanuddin Ima Nur Hadisah Ilham Irwandi Rahmat M. Rajab Sutra Mijaya Mamin, Ratnawati Muh. Tawil Muthiah AT. Nur Arfa Yanti Nurfitra Yanto Ramlawati, Ramlawati Ratnawaty Mamin Ratnawaty Mamin Ratnawaty Mamin Riska Amriani Saenab, Sitti Sahribulan Sahribulan Salma Samputri Salmiah Hardianty Sitti Rahma Yunus Sitti Saenab Sitti Saenab St. Mutia Alfiyanti Muhiddin Suci Muliana Sutireski Ibrahim Triana Nurfitra Uswatun Nisa

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