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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Makara Journal of Science
Latifah, Vida Rahma
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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INTRA-SPESIFIC DIVERSITY OF BUTTERFLY PEA (Clitoria ternatea L.) REVEALED BY ISSR WITH INVARIABLE ITS RECORDS Yusuf, Adib Fakhruddin; Latifah, Vida Rahma; Nurcahyati, Vivi Indah; Nurhasanah, Anggun Diyan; Widyasari, Adristi Shafa; Nainggolan, Ananto Puradi; Hasibuan, Aldy Riau Wansyah; Hidayat, Madyan Akmal; Karmilah, Karmilah; Pratiwi, Arini Dian; Aurantika, Rindu; Hasanah, Muslifah; Aristya, Ganies Riza; Handayani, Niken Satuti Nur; Arisuryanti, Tuty; Lesmana, Indra; Daryono, Budi Setiadi
BIOTROPIA Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.1.2362

Abstract

Article Highlights:- The ITS region exhibits no genetic variation among butterfly pea genotypes.- ISSR reveals high polymorphism, proving effective for genetic diversity analysis.- White single and purple single genotypes show the greatest genetic distance.- ISSR-6 ((CT)₈TG) demonstrates the highest polymorphism and reliability for assessment.- The ITS sequences have been deposited in GenBank under the accession IDs PQ198055–PQ198061. ABSTRACTClitoria ternatea L., a perennial plant in the Fabaceae, is recognized for its resilience in tropical climates and its diverse applications in both culinary and medicinal fields. However, the limited exploration of its genetic diversity constrains breeding efforts aimed at improving desirable traits. This limitation highlights the need to optimize selection strategies, identify superior genotypes, and preserve valuable genetic resources for long-term conservation and crop enhancement. This study aimed to explore genetic variation using molecular markers to analyze C. ternatea genotypes based on petal architecture and color differences. To assess the genetic diversity of C. ternatea, Sanger sequencing of the Internal Transcribed Spacer (ITS) region and Inter-Simple Sequence Repeat (ISSR) markers were applied to seven wild populations from Sleman, Yogyakarta. The ITS region exhibited no genetic variation, indicating its conserved nature and limited ability to differentiate genotypes. In contrast, ISSR markers effectively detected genetic variation, identifying 62 polymorphic fragments out of 162 total bands. The highest genetic distance (0.297) was observed between the WS and PS genotypes, whereas the double-petal genotypes (WD and PD) displayed the closest phenetic relationship. Among the ISSR primers, UBC-808, UBC-812, and ISSR-6 exhibited high PIC and RP values, confirming their reliability in genetic diversity analysis. These results underscore the utility of ISSR markers as a robust tool for genetic diversity assessment, offering valuable insights for breeding programs and germplasm conservation in C. ternatea.
Gama Melon Parfum, a Potential Melon Genotype Tolerant of Salinity Stress Nugroho, Giri; Chikmawati, Tatik; Sartika, Dian; Yusuf, Adib Fakhruddin; Putro, Karso Suryo; Latifah, Vida Rahma; Daryono, Budi Setiadi; Wibowo, Anjar Tri; Nurhayati, Awik Puji Dyah; Meitha, Karlia; Miftahudin, Miftahudin
Makara Journal of Science Vol. 29, No. 3
Publisher : UI Scholars Hub

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Abstract

Melona, Tacapa Gold, and Gama Melon Parfum are novel Melon genotypes that have been introduced for cultivation in the saline-prone coastal region of Bocor Beach, Kebumen, Central Jawa. Elevated salt concentrations in the area pose challenges, impacting yield. The potential of these new genotypes for saline land remains unexplored. This study aimed to assess the morphophysiological and molecular responses of these genotypes to salinity stress. Seeds were germinated in cocopeat and ABmix 3000 solution and treated with 220 mM NaCl until harvest. The analysis included morphology, chlorophyll content, proline accumulation, malondialdehyde levels, and gene expression were analyzed. After 1 week of salinity stress, the results showed reduced plant height, leaf number, stomatal density, percentage of opened stomata, and width and length of leaves. The malondialdehyde, proline, and root-shoot dry mass ratio increased. The expression of CSD1-1, P5CR, and late embryogenesis abundant genes increased under salinity treatment. Gama Melon Parfum exhibits salt tolerance, as indicated by higher P5CR gene expression, proline accumulation, and leaf-relative water content. P5CR gene expression, proline accumulation, and leaf-relative water content are valuable indicators of salt tolerance for assessing melon genotypes.
Co-Authors Anjar Tri Wibowo Aurantika, Rindu Awik Puji Dyah Nurhayati BUDI SETIADI DARYONO Ganies Riza Aristya Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hidayat, Madyan Akmal Indra Lesmana Karmilah, Karmilah Meitha, Karlia Miftahudin . Nainggolan, Ananto Puradi Niken Satuti Nur Handayani Nugroho, Giri Nurcahyati, Vivi Indah Nurhasanah, Anggun Diyan Pratiwi, Arini Dian Putro, Karso Suryo Sartika, Dian TATIK CHIKMAWATI Tuty Arisuryanti, Tuty Widyasari, Adristi Shafa Yusuf, Adib Fakhruddin