I Made Kardena
Laboratorium Patologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia

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COCCIDIOSIS IN BROILER CHICKENS RAISED IN CLOSE HOUSE IN PATAS VILLAGE, GEROKGAK, BULELENG Wayan Gede Ananta Brahmananda; I Putu Cahyadi Putra; I Made Kardena; Hapsari Mahatmi; I Nyoman Mantik Astawa
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p14

Abstract

Coccidiosis is a parasitic disease of chickens caused by the protozoan Eimeria spp. This disease economically causes many problems and losses in chicken farms, such as inhibiting growth and reducing productivity, ultimately resulting in death. Coccidiosis can attack chickens on farms that are kept with a closed-house or open-house system. Close-house farms generally have good biosecurity, but this does not rule out the possibility of a disease that attacks chickens, one of which is coccidiosis. The purpose of this case report is to report the incidence of coccidiosis in a farm with a closed-house system. The animal used as a case was a 21-day-old broiler chick from a close-house farm in Patas Village, Gerokgak, Buleleng, Bali. Clinical signs of case chickens were anorexia, dull feathers, pale wattle, weakness, separation from the flock, and faeces mixed with blood with a liquid consistency. The examination methods carried out were epidemiological investigations by calculating morbidity, mortality and case fatality rate (CFR), anatomical pathology, histopathology, parasitology (qualitative and quantitative examination of faeces), and bacteriology. The results of the epidemiological investigation consisted of morbidity of 0.5%, mortality of 0.13%, and CFR of 23%. An anatomical pathology examination found bleeding in the cecum. Histopathological examination of the cecum found haemorrhage, erosion, inflammatory cell infiltration, and schizonts in the lamina propia. Faecal examination revealed the presence of Eimeria sp. oocysts with a concentration of 234,200 oocysts/gram. Bacterial examination showed Escherichia coli growing from cecum and colon samples which are normal flora. Based on all laboratory examinations performed, the case chicken was infected by Eimeria sp. with a severe degree of infection. The advice for farmers is to improve sanitation and biosecurity to minimize the risk of contamination by coccidiosis. If sick chickens are found, treatment with antiprotozoal containing toltrazuril and diclazuril should be given.
COMPARISON OF ANTIBODY TITERS IN BROILERS GIVEN GREEN MENIRAN EXTRACT AT DIFFERENT DOSES FOR TWO WEEKS BEFORE NEWCASTLE DISEASE BOOSTER VACCINATION Dewa Kade Agung Hendra Putra Dinata; Ida Bagus Kade Suardana Suardana; I Ketut Suada; Ni Luh Eka Setiasih; I Made Kardena; Tjokorda Sari Nindhia
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p26

Abstract

Newcastle Disease (ND) is a viral disease that affects poultry, including broilers. One of the preventive measures is vaccination; however, its effectiveness remains suboptimal, necessitating an alternative approach such as green meniran (Phyllanthus niruri Linn.) extract to enhance antibody titers. This study aims to determine the differences in antibody titers in broilers administered green meniran extract at different doses for two weeks before the ND booster vaccination. The study involved 30 broilers aged 1 to 32 days. The experimental design followed a Completely Randomized Design with a hierarchical pattern consisting of three treatment groups. Serum samples were collected one day before the ND booster vaccination (17 days old), oneweek post-booster vaccination (25 days old), and two weeks post-booster vaccination (32 days old). The serum samples were tested using the Hemagglutination Inhibition (HI) assay. Data were analyzed using Analysis of Variance (ANOVA) and regression analysis. ANOVA results showed that the treatment group (P2) receiving green meniran extract at a dose of 6 mg/kg BW/day exhibited a significant increase (P<0.05) compared to the treatment group (P1) receiving 4 mg/kg BW/day. Regression analysis indicated that each group experienced an increase in antibody titers up to the fourth week post-booster vaccination. The findings suggest that different sampling times significantly (P<0.05) influenced the antibody titers formed. In conclusion, green meniran extract as a natural immunostimulator can enhance the immune response in broiler chickens. The combination of green meniran extract at doses of 4 mg/kg BW/day and 6 mg/kg BW/day for two weeks with ND booster vaccination proved to be more effective in increasing ND antibody titers in broilers than vaccination alone.
HISTOLOGIC FEATURES OF GRANULOCYTE WHITE BLOOD CELLS AND PLATELET DISTRIBUTION WIDTH VALUES IN DOGS WITH DERMATITIS Patricia Amelia Debianti Wagut; Ni Ketut Suwiti; Sri Kayati Widyastuti; Nyoman Sadra Dharmawan; Ni Luh Eka Setiasih; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p35

Abstract

Dermatitis in dogs is an inflammation of the skin caused by parasites, bacteria, fungi and metabolic disorders, with severity varying from mild to severe. This condition triggers inflammation that affects granulocyte leukocytes, namely neutrophils, eosinophils and basophils, which can undergo necrosis. Necrosis is characterized by changes in nuclear morphology, such as pycnosis, karyorexis, and karyolysis due to irreversible cell injury. In addition, Platelet Distribution Width (PDW) values reflect variations in platelet size and are often associated with inflammatory activity. This study analyzed the histological features and differences in necrotizing leukocyte counts and PDW values in dogs with mild and severe dermatitis. The results showed that necrotizing neutrophils in mild dermatitis (4.9 ± 5.2) were lower than those in severe dermatitis (5.4 ± 3.3), but the results of the independent t-test showed that the difference was not significant (P > 0.05). The opposite situation in eosinophils and basophils, where eosinophils that experienced necrosis in mild dermatitis (2.5 ± 11) were higher than those in severe dermatitis (0 ± 0), while basophils that experienced necrosis were higher in mild dermatitis (9.6 ± 17) than in severe dermatitis (0 ± 0), but the results of the independent t-test showed that the difference was significant (P < 0.05). The PDW value in mild dermatitis (15 ± 2.4) was greater than that in severe dermatitis (14.5 ± 2), but not significantly different (P > 0.05), it can be concluded that the severity of dermatitis does not affect platelet size. Further research needs to be done on health status by looking at other indicators such as the presence of lymphocytes and monocytes in dogs with mild derrmatitis and severe dermatitis.
HISTOLOGICAL FEATURES OF LYMPHOCYTES, MONOCYTES AND HEMOGLOBIN ONCENTRATIONS IN DOGS WITH MILD AND SEVERE DERMATITIS Ni Putu Dewi Rahmawati; Ni Ketut Suwiti; Sri Kayati Widyastuti; Nyoman Sadra Dharmawan; Ni Luh Eka Setiasih; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p30

Abstract

Dogs are susceptible to various dermatological disorders, among which dermatitis is commonly observed. This condition may be caused by fungal infections, ectoparasites, bacterial agents, or metabolic disorders. Dermatitis presents with clinical manifestations ranging from mild to severe, often characterized by widespread skin lesions. The associated inflammatory response may induce alterations in the immune system, particularly affecting agranulocytic leukocytes namely lymphocytes and monocytes or leading to cellular necrosis. Severe dermatitis is frequently accompanied by secondary infections, which may elevate the risk of inflammation-induced anemia and result in changes to hemoglobin (HGB) levels. This study aims to investigate the histological features of necrotic lymphocytes and monocytes, as well as hemoglobin (HGB) levels, in dogs affected by mild and severe dermatitis. Histological examination revealed that necrotic lymphocytes and monocytes exhibited signs of pyknosis, karyorrhexis, and karyolysis. The number of necrotic lymphocytes in dogs with mild dermatitis (10±4.64) was slightly higher than in those with severe dermatitis (8.65±3.38), although the difference was not statistically significant (P˃0.05). In contrast, the number of necrotic monocytes in mild dermatitis cases (19.3±20.8) was significantly greater than in severe cases (3.35±11.62) (P˂0.05). Hemoglobin levels in dogs with mild dermatitis (10.75±4.23) were marginally lower than those in severe cases (11.23±2.9), with no statistically significant difference (P˃0.05).
ISTOPATHOLOGY OF THE BRAIN OF WHITE RATS UPON ADMINISTRATION OF MIMOSIN FROM SIMPLISIA OF LEUCAENA LEAVES Yuyun Setia Ningsih; I Ketut Berata; Ni Luh Eka Setiasih; I Made Kardena; Samsuri; I Made Merdana
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p46

Abstract

Lamtoro (Leucaena leucocephala) is a widely used cattle feed in Indonesia due to its high protein, flavonoid, and tannin content. However, it contains mimosine, a compound whose effects on the liver remain poorly understood. This study aimed to investigate the histopathological effects of mimosine on the liver of experimental animals. Twenty male Wistar rats (Rattus norvegicus), aged 2 months (150–200 g), were divided into four groups: a negative control (P0), a positive control given pure mimosine (5 mg/head/day, P1), and two groups administered mimosine from lamtoro leaf simplisia at 50 mg/head/day (P2) and 150 mg/head/day (P3). Treatments were administered orally for 14 days. On day 15, liver samples were collected, fixed in 10% neutral buffered formalin (NBF), and processed for histopathological analysis using hematoxylin-eosin (H&E) staining. Lesions (congestion, inflammation, and necrosis) were scored on a scale of 0 (normal) to 3 (severe). Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests. Results showed that pure mimosine (5 mg) and lamtoro simplisia (50 mg and 150 mg) induced significant hepatic congestion compared to controls, while inflammation was not statistically significant. Necrosis was not observed in any group. No significant difference was found between the two doses of lamtoro simplisia. These findings suggest that mimosine, whether pure or derived from lamtoro leaves, induces mild to moderate liver damage, primarily congestion, but further research is needed to clarify its long-term effects.
COMPLICATIONS OF CANINE PARVOVIRUS INFECTION WITH STREPTOCOCCUS CANIS IN A FREE-ROAMING MIXED BREED DOG: A CASE STUDY WITH ANATOMICAL PATHOLOGY AND HISTOPATHOLOGICAL Ashley; Gusti Ayu Yuniati Kencana; I Nengah Kerta Besung; I Made Dwinata; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p04

Abstract

Canine Parvovirus (CPV) is a highly contagious infectious agent in dogs, with high morbidity and significant mortality rates, especially in unvaccinated animals. This study reports a case of CPV infection exacerbated by secondary bacterial pneumonia caused by Streptococcus sp. in a 10-month-old male mixed-breed dog in Denpasar, Bali. The dog exhibited clinical symptoms for four days, including bloody diarrhea, dehydration, anorexia, coughing, dyspnea, vomiting, and nasal discharge, before succumbing on the fifth day. Diagnosis was conducted through histopathological examination, Polymerase Chain Reaction (PCR), and bacterial culture. PCR results confirmed the presence of CPV with a 900 bp fragment, while bacteriological analysis identified Streptococcus sp. in lung tissue with hemolytic activity. Macroscopic analysis revealed hemorrhagic pneumonia, edematous myocarditis, and hemorrhagic enteritis, while histopathology showed inflammatory cell infiltration and tissue necrosis. Streptococcus infection worsened the dog's condition by causing severe lung inflammation, impairing respiratory function, and increasing the risk of death. The combination of CPV and secondary infection highlights the importance of vaccination in preventing CPV and the need for early detection through PCR and bacterial culture to improve prognosis and treatment effectiveness. This study emphasizes that rapid diagnosis and appropriate supportive therapy can help reduce disease severity and lower mortality rates in infected dogs.
THE THE EFFECT OF GREEN MENIRAN EXTRACT ON AVIAN INFLUENZA ANTIBODY TITER IN BROILERS TWO WEEKS BEFORE VACCINATION Dewandaru Evan Wintyassatya; Ida Bagus Kade Suardana; Romy Muhammad Dary Mufa; I Made Sukada; Ni Luh Eka Setiasih; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p32

Abstract

Avian Influenza (AI) is a viral disease that poses a serious threat to poultry farming, particularly in broiler production. The rapid and massive spread of AI in poultry flocks can cause substantial economic losses for farmers. Vaccination is one of the primary strategies for AI prevention; however, the currently available AI vaccines are killed/inactivated types, which tend to induce antibody titers slowly. This limitation, coupled with the relatively short rearing period of broilers, reduces vaccine effectiveness in broiler chickens. This study aimed to evaluate the effect of green meniran (Phyllanthus niruri Linn.) extract on enhancing antibody titers in broilers when administered for two weeks prior to AI vaccination. A total of 30 broiler chickens aged from day 1 to 32 days were used as experimental subjects. The study employed a Completely Randomized Design (CRD) with three groups: negative control (K−), positive control (K+), and treatment group (P). Data were analyzed using Analysis of Variance (ANOVA) and Regression Analysis via SPSS software. The results demonstrated a comparative increase in average antibody titers in the treatment group receiving Phyllanthus niruri extract compared to the control groups. It was concluded that supplementation with Phyllanthus niruri extract effectively enhanced antibody titers in broilers vaccinated with the inactivated AI La Sota vaccine, with peak antibody responses observed 1–2 weeks post-vaccination. The administration of green Phyllanthus niruri extract has been proven to stimulate antibody titers in broilers by accelerating the development of protective antibody levels against the AI virus. The timing of sample collection was shown to significantly affect antibody titers, with the highest levels observed during the first to second week post-vaccination. Future research on the administration of Phyllanthus niruri extract to improve antibody titers against Avian Influenza (AI) in broilers is expected to extend the duration of supplementation until the harvesting period and to include higher dosage variations in order to achieve more optimal and protective results.