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UJI AKTIVITAS ANTIBAKTERI EKSTRAK BUAH DAN DAUN MENGKUDU (Morinda citrifolia L.) TERHADAP BAKTERI PENYEBAB JERAWAT (Staphylococcus epidermidis) D Prasetyorini; Novi Fajar Utami; Alfi Syari Sukarya
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 9, No 2 (2019): FITOFARMAKA | Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Jerawat merupakan penyakit pada permukaan kulit. Terjadi akibat tersumbatnya folikel polisebase, disebabkan oleh infeksi bakteri (Staphylococcus epidermidis). Tanaman yang memiliki potensi sebagai agen antibakteri adalah tanaman mengkudu, yang terbukti memiliki kandungan senyawa antrakuinon, alkaloid dan flavonoid. Penelitian ini bertujuan untuk menentukan karakteristik ekstrak buah dan daun mengkudu serta aktivitas antibakteri terhadap bakteri Staphylococcus epidermidis. Dengan menggunakan metode ekstraksi maserasi etanol 96%. Pengujian Lebar Daerah Hambat (LDH) dengan metode difusi kertas cakram dan Konsentrasi Hambat Minimum (KHM) dengan metode dilusi agar. KHM pada konsentrasi 25%, 30%, 35% dan 40%. LDH dilakukan pada konsentrasi 40%, 50% dan 60%. Hasil ekstrak etanol 96% buah dan daun mengkudu memiliki KHM pada konsentrasi 40% dan LDH buah 3,1 mm pada konsentrasi 50% dan 60%. Ekstrak daun mengkudu memiliki LDH 2,5 mm pada konsentrasi 60%.

TOKSISITAS BEBERAPA EKSTRAK RIMPANG CABANG TEMULAWAK (Curcuma xanthorrhiza Roxb.) PADA LARVA UDANG (Artemia salina Leach) Prasetyorini Prasetyorini; Ike Yulia Wiendarlina; Anisa Bela Peron
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 1, No 2 (2011): FITOFARMAKA
Publisher : Universitas Pakuan

Pengujian toksisitas beberapa ekstrak rimpang temulawak hasil ekstraksi denganmetode yang berbeda telah dilakukan terhadap larva udang Artemia salina denganmenggunakan Brine Shrimp Lethality Test (BSLT). Ekstraksi dilakukan dengan metodemaserasi, sokletasi dan refluks. Pelarut yang digunakan untuk ekstraksi adalah etanol 96%.Toksisitas diukur dengan menghitung jumlah larva udang yang mati, kemudian nilai LCuntuk setiap ekstrak ditentukan dengan menggunakan Probit Analisis Method. Hasilpenelitian menunjukkan bahwa nilai LCekstrak yang diperoleh dengan metode maserasi,soxhlet dan refluks berturut-turut adalah 14.87 ppm, 19.13 ppm dan 35.92 ppm. Ekstrakrimpang temulawak dengan metode maserasi merupakan ekstrak teraktif. Hasil penapisanfitokimia menunjukkan bahwa dalam ekstrak hasil maserasi tersebut dapat diidentifikasiadanya senyawa golongan alkaloid, flavonoid, steroid, kuinon dan triterpenoid.Kata kunci : Curcuma xanthorrhiza Roxb., toksisitas, Artemia salina Leach

POTENSI EKSTRAK REFLUKS KULIT BATANG KAYU MANIS SEBAGAI ANTIJAMUR Candida albicans dan Candida tropicalis Prasetyorini Djarot; Novi Fajar Utami; Yulianita Yulianita; Novi Novitasari; Widya fitriyani
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 11, No 2 (2021): FITOFARMAKA | Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jf.v11i2.2722

Kayu manis (Cinnamomum burmannii) merupakan salah satu tanaman dari famili Lauraceae yang mempunyai potensisebagai antijamur penyebab kandidiasis seperti C.albicans dan C.tropicalis, hal ini disebabkan kulit batang kayu manis mengandung minyak atsiri, flavonoid, polifenol, saponin, dan tannin. Tujuan penelitian adalah mendapatkan obat alternatif untuk mengobati penyakit yang disebabkan oleh jamur C.albicans dan C.tropicalis. Metode esktraksi yang digunakan adalah refluks, menggunakan pelarut yang kepolaran berbeda yaitu n-Heksan (non polar), etil asetat (semi polar), dan etanol 96% (polar). Ekstrak yang diperoleh selanjutnya dilakukan skrining kualitatif untuk kandungan flavanoid, alkaloid, tanin, polifenol dan saponin, diuji daya antioksidanya dan dilakukan penetapan kadar polifenol. Pengujian aktivitas antijamur dilakukan dengan menentukan Konsentrasi Hambat Minimum (KHM) dengan metode dilusi agar dan pengujian Lebar Daerah Hambat (LDH) menggunakan metode difusi kertas cakram. Hasil penelitian menunjukkan bahwa pelarut etanol 96% menunjukan kadar polifenol tertinggi yaitu 3,89% dan aktivitas antioksidan terbaik yaitu 66,29 mg/L, kstrak n-heksan mempunyai nilai KHM terbaik 2% dan LDH terbaik pada konsentrasi 8% dengan nilai rata-rata 16.30 mm±0.28 terhadap pertumbuhan C.albicans dan 15.66 mm ± 0.28 terhadap C.tropicalis.

UJI ANTIBAKTERI EKSTRAK DAUN SAMBUNG NYAWA (Gynura procumbens (Lour.) Merr.) DAN DAUN TAPAK LIMAN (Elephantopus scaber L.) TERHADAP Salmonella thypi Prasetyorini Djarot; Anggita Rahmadini; Novi Fajar Utami
EKOLOGIA Vol 19, No 1 (2019): EKOLOGIA: JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

This research was aimed to create aromatic candle, infused with cinnamon (Cinnamomum burmanii) bark essential oil, which is a potential repellant for house fly (Musca domestica). The distillation of akar wangi samples was carried out at the Spice and Aromatic Research Unit by vapor distillation method for 9-12 hours. The aromatic candles were based on a mixture of solid paraffin and stearin in a 2:8 proportion, respectively, enriched with fragrant root bark essential oils with 5 formulas, namely F1 (placebo), F2 (fixative), F3 (1% essential oil), F4 (2% essential oil), and F5 (3% essential oil). The repellency test was carried out by inserting 20 house flies in a of 50x50x50 cm container that contains a lighted aromatic candle according to the doses mentioned above, and shrimp scalps located 15 cm from the candle that functions as a bait. The calculated parameters were the hourly number of flies that were allured to the bait for 6 hours, which were related to the candle’s repellency and the house fly’s preference of candle. The repulsion ability result were 89.724%, 75.28%, 68.06%, 49.17%, 9.72% for F5, F4, F3, F2, and F1, respectively. The result also showed that the F4 sample was the most preferred sample.

LILIN AROMATIK MINYAK ATSIRI KULIT BATANG KAYU MANIS (Cinnamomum burmannii) SEBAGAI REPELEN LALAT RUMAH (Musca domestica) Prasetyorini Djarot; Moerfiah .; Dwi Ambarwati
EKOLOGIA Vol 19, No 2 (2019): EKOLOGIA: JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

This research was aimed to create aromatic candle, infused with cinnamon (Cinnamomum burmanii) bark essential oil, which is a potential repellant for house fly (Musca domestica). The distillation of akar wangi samples was carried out at the Spice and Aromatic Research Unit by vapor distillation method for 9-12 hours. The aromatic candles were based on a mixture of solid paraffin and stearin in a 2:8 proportion, respectively, enriched with fragrant root bark essential oils with 5 formulas, namely F1 (placebo), F2 (fixative), F3 (1% essential oil), F4 (2% essential oil), and F5 (3% essential oil). The repellency test was carried out by inserting 20 house flies in a of 50x50x50 cm container that contains a lighted aromatic candle according to the doses mentioned above, and shrimp scalps located 15 cm from the candle that functions as a bait. The calculated parameters were the hourly number of flies that were allured to the bait for 6 hours, which were related to the candle’s repellency and the house fly’s preference of candle. The repulsion ability result were 89.724%, 75.28%, 68.06%, 49.17%, 9.72% for F5, F4, F3, F2, and F1, respectively. The result also showed that the F4 sample was the most preferred sample.

Peningkatan Keterampilan Industri Rumah Tangga Bagi Ibu-Ibu Rumah Tangga Di Desa Cibadak, Kecamatan Ciampea, Kabupaten Bogor Melalui Pelatihan Dan Pendampingan Pembuatan Virgin Coconut Oil (VCO) Prasetyorini Prasetyorini; Moerfiah Moerfiah; Iyan Mulyana
Patria : Jurnal Pengabdian Kepada Masyarakat Vol 1, No 2: September 2019
Publisher : Universitas Katolik Soegijapranata

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24167/patria.v1i2.2307

Abstrak — Desa Cibadak adalah Desa yang terletak di Kecamatan Ciampea, Kabupaten Bogor. Salah satu kendala di bidang ekonomi di Desa Cibadak adalah pada aspek industri, terutama industri rumah tangga. Berdasarkan masalah ini, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Pakuan melakukan kegiatan Pengabdian Masyarakat yang dimulai dengan kegiatan untuk meningkatkan keterampilan ibu rumah tangga di Desa Cibadak untuk menghasilkan industri rumah tangga melalui pelatihan dan pelatihan pendampingan dalam pembuatan Virgin Coconut Oil (VCO). Melalui kegiatan ini, diharapkan ibu rumah tangga tidak hanya memiliki keahlian untuk membuat produk kreatif dari industri rumah tangga tetapi dapat memasarkannya sehingga di masa depan mereka dapat membantu kesejahteraan ekonomi keluarga. Kegiatan telah dilakukan dari tanggal 19 April 2019 hingga 16 Juni 2019. Kegiatan tersebut dihadiri oleh 20 ibu rumah tangga di wilayah Desa Cibadak. Hasil evaluasi dari kegiatan yang telah dilakukan menunjukkan peningkatan keterampilan ibu rumah tangga di Desa Cibadak untuk menghasilkan produksi industri rumah tangga yang diharapkan dapat meningkatkan ekonomi keluarga. Salah satunya adalah peningkatan kemampuan peserta untuk membuat VCO.Abstract — Desa Cibadak is a Desa located in Kecamatan Ciampea, Kabupaten Bogor. One of the obstacles in the economic field in Desa Cibadak is in the industrial aspects, especially the home industry. Based on these problems the Faculty of Mathematics and Natural Sciences of Universitas Pakuan carried out Community Service activities which began with activities to improve the skills of housewives in Desa Cibadak to produce home industries through training and mentoring training in making Virgin Coconut Oil (VCO). Through these activities, it is expected that housewives not only have the expertise to make creative products from home industries but can market them so that in the future they can help the family's economic prosperity. Activities have been carried out from the date of April 19, 2019 to June 16, 2019. Activities were attended by 20 housewives in the Desa Cibadak area. The results of the evaluation of the activities that have been carried out indicate an increase in the skills of housewives in Desa Cibadak to produce home industry production which is expected to improve the family economy. One of them is the increase in the ability of participants to make VCO.

Implementation Of Jigsaw Coopertaive Learning And Gallery Walk Based On Practices To Develop Science Process Skills And Sscientific Attitude Of Students Fitri Laila; Prasetyorini Prasetyorini; Kurniasih Kurniasih
JSEP (Journal of Science Education and Practice) Vol 5, No 1 (2021): June 2021
Publisher : Universitas Pakuan

This study aims to obtain information on improving students ' science process skills and scientific attitudes through practical - based Jigsaw and Gallery Walk learning on the material organization of life. This research method uses a quasi-experimental design with a two-experimental pre-posttest group design, where no one acts as a control group, both groups act as an experimental group that is given different treatment and both are given pretest and posttest. The object of this research is the students of class VII.1 and VII.2 with 41 students each. The instruments used are in the form of multiple choice test questions, KPS observation sheets or performance assessments and questionnaires for scientific attitudes before and after learning which are equipped with learning implementation observation sheets and student and teacher questionnaires. The results of this study indicate that: (1) there are differences in the KPS of students who carry out practicum-based Jigsaw and Gallery Walk learning. (2) there are differences in the scientific attitude of students who carry out practical-based Jigsaw and Gallery Walk learning. (3) practicum-based Jigsaw and Gallery Walk learning can develop students' KPS and Scientific Attitudes. Based on the normalized gain scores, these two learnings have moderate values, both on the KPS and students' scientific attitudes. Thus, it can be concluded that Jigsaw and Gallery Walk learning can improve students' KPS and Scientific Attitudes.

Inquiries and Problem Based Learning (Pbl) Assistant Multimedia Learning Models in Improving Students' Critical Thinking Skills Idah Paridah; Prasetyorini Prasetyorini; Dadang Jaenudin
JSEP (Journal of Science Education and Practice) Vol 5, No 2 (2021): December 2021
Publisher : Universitas Pakuan

This study aims to determine the interaction between the multimedia-assisted inquiry learning model and the multimedia-assisted Problem Based Learning (PBL) model on critical thinking skills and to determine the differences in critical thinking skills between students using inquiry learning and PBL. This study used a quasi-experimental with static group pre-posttest design. The implementation of the learning involved students of class XI IPA who were studying the reproductive system, respectively 37 students for the inquiry class and 33 students for the PBL class. The instrument used was an essay question consisting of 10 questions, and a questionnaire to collect student and teacher responses. The results showed that the inquiry learning model and PBL were able to improve students' critical thinking skills, and overall, the PBL model is better than the inquiry learning model. Students and teachers gave a positive response to the inquiry learning model and PBL.

Learning Science With Group Investigation And Guided Inquiry To Increase Biological Literacy In Coordination System Materials Of Class Science Xi Students Hariyanti Nenti; Prasetyorini Prasetyorini; Suhardi Eka
JSEP (Journal of Science Education and Practice) Vol 5, No 1 (2021): June 2021
Publisher : Universitas Pakuan

The aims of this research are to find out : the effect difference between implementation of cooperative learning model's Group Investigation and type, and Guided Inquiry model's to Increase Biology Literacy , and the effect interaction between implementation of cooperative learning model's and student's on the learning motivation to Increase Biology Literacy. The research was in the Senior High School in the Rancabungur District academic year 2016/2017. The research method used is a quasi-experimental method with a two factorial design. The population of the research is students at the twelth grade Senior High School in the Rancabungur District. The samples of research consist of 80 students. Instruments used for collecting data are achievement test of Biology and the questionnaires of study motivation. To get appropriate and accuracy of the Biology achievement test Technique of t test with significance level of = 0.05. The result of the research are as follows : there is a significant difference in effect of implementation of cooperative learning Group Investigation (GI) and type, and Guided Inquiry model's on Increase Biology Literacy (F h Ft , 0.05 = 2.58 1.99). The questionnaire results showed that students and teacher responded positively to the Group Investigation and Group Inquiry Learning Model. Based on those findings, the research concludes that learning achievement of Biology can be increased throught learning models and improving the student's achievement motivation. The improving of learning quality is able to be achieved through the use of cooperative learning model's Group Investigation and type, and Guided Inquiry model's and by pay attention to the student's Increase Biology Literacy.

Abstract Guided Inquiry Learning with Mind Mapping and Concept Map to Increase Concept Mastery and Student Learning Motivation Diah Mustikarini; S. Kurniasih; Prasetyorini Prasetyorini
JSEP (Journal of Science Education and Practice) Vol 5, No 2 (2021): December 2021
Publisher : Universitas Pakuan

This study aims to measure students' conceptual mastery and motivation in learning the respiratory system. The study was conducted on class VIII SMPN 1 Klapanunggal students in 2017/2018. The learning used in this study was through guided inquiry learning with mind mapping with pretest posttest control group design. The use of this research design involves two classes as research samples, one experimental class and one control class. The selection of these two classes is done by taking subjects not based on strata, random or regional but based on certain goals. The research subjects were divided into two groups, namely the experimental group and the experimental group. control. The experimental group received Guided Inquiry learning with mind mapping, while the control group was the group that received Guided Inquiry learning with concept maps. The results of mastery of the concept of the experimental class and control class each obtained an average gain of 6.09 and 5.78, the average pretest score of the experimental class students was 51.9 and the control class students were 44.7 from the ideal score. This shows that the average pretest score of the two classes is not much different. It can be concluded that the average N-gain in the control class is 36.9% in the medium category, while in the experimental class the average N-gain is 43.7% in the medium category.

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