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Jurnal Bios Logos
Published by Universitas Sam Ratulangi
ISSN : 20889569     EISSN : 26563282     DOI : https://doi.org/10.35799/jbl.11.2.2020.28153
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
JURNAL BIOS LOGOS is the journal published by Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. The aims of the journal are to publish original research papers and article review in biology science i.e. botany, zoology, molecular biology, microbiology, ecology, diversity and conservation, taxonomy and biogeography. BIOS LOGOS is published two times per year (February and August)
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 14 Documents
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Search results for , issue "Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS" : 14 Documents clear
Antagonist Activity of Gliocladium sp. Against Fusarium Wilt Fungi in Chili Plant (Capsicum frutescens L.) Cheryl Pijoh; Feky Recky Mantiri; Song Ai Nio
JURNAL BIOS LOGOS Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i2.62910

Abstract

Fusarium sp. is a pathogen that causes wilt disease in chili plant (Capsicum frutescens L.), which has a significant impact on crop production. One of the environmentally friendly control strategy is the use of biological agents, such as Gliocladium sp. This study aimed to evaluate the antagonistic ability of Gliocladium sp. to inhibit the growth of Fusarium sp. in vitro. The experiment was conducted using the dual culture method on Potato Dextrose Agar (PDA) medium. Fusarium sp. isolates were obtained from the culture collection of BPPMTPH Kalasey. Observations were carried out of seven days to assess colony growth and calculate the percentage of inhibition. Gliocladium sp. demonstrated the ability to inhibit Fusarium sp. growth, with a maximum inhibition percentage of 51.52% on the seventh day. Gliocladium sp. exhibited rapid growth and dominated the medium, covering the pathogen colony entirely, although no clear inhibition zone was formed. The antagonistic mechanism is presumed to involve competition for space and nutrients, and parasitism. The mechanism of competition was demonstrated by the ability of Gliocladium sp. to grow more rapidly and dominate the PDA medium compared to Fusarium sp. in the dual culture treatment. Parasitism was identified through the coiling of Fusarium sp. hyphae by Gliocladium sp. hyphae, accompanied by morphological abnormalities observed in Fusarium sp. These findings indicate that Gliocladium sp. has potential to be developed as a biological control agent against Fusarium wilt desease in chili plants.
Metabolite Profiling of Kombucha Fermented Beverage with Substrate Variation Agustina Monalisa Tangapo; Pience Veralyn Maabuat; Beivy Kolondam; Lalu Wahyudi; Susan Marlein Mambu; Marsia Pasassa
JURNAL BIOS LOGOS Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i2.63719

Abstract

Kombucha is a fermented beverage of tea and sugar with symbiotic culture of bacteria and yeast (SCOBY/symbiotic culture of bacteria and yeast). Changes in chemical composition which are functional constituents and flavor components of kombucha beverages can occur during kombucha fermentation. Substrate is one of the factors determining the characteristics of kombucha. Generally, the substrate used in making kombucha comes from Camellia sinensis tea leaves. Modification of substrates for kombucha fermentation with herbs can increase the various potentials of kombucha. The aim of this study was to analyze untargeted metabolomics for metabolite profiling of kombucha fermented from different substrates (clove leaves, guava leaves, mint leaves, and morel berry leaves). The research methods used were controlled fermentation for fourteen days, and metabolomic analysis using Gas Chromatography-Mass Spectrometry. The results showed that different substrates affected the metabolite content profile of fermented kombucha. The metabolite profile of Gas Chromatography-Mass Spectrometry results identified from clove kombucha as many as 26 metabolites, from guava kombucha 13 metabolites, from mint leaf kombucha 24 metabolites, and morel berry leaf kombucha with 21 metabolites. There is a metabolite identified from each kombucha with these substrate variations, namely 5-Hydroxymethylfurfural.
The Potential Toxicity of Animal Oils to Pathogenic Fungi Nila Fish (Oreochromis niloticus) Deidy Y Katili; Marina Flora Oktavine Singkoh; Frans Bernhard Rondonuwu; Stella Deiby Umboh; Marnix L D Langoy; Vivi B Montong
JURNAL BIOS LOGOS Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i2.63830

Abstract

Nila is a type of freshwater fish that has high economic value, as a source of animal protein for the community, and is an important commodity in the freshwater fish business. Nila is also one of the main commodities that contributes to the increase in aquaculture production. Nila farming often faces the problem of declining yields due to diseases. One of the diseases that is very detrimental to freshwater fish is diseases caused by fungi. The purpose of this study is to test the toxicity of animal oil in inhibiting the growth of Nila pathogenic fungi. Isolation of pathogenic fungi in fish is carried out by cutting the infected parts (scales, fins, and gills) with a size of about 1x1cm. After pure culture is carried out on each pathogenic fungus, then macroscopic and microscopic identification is carried out. Animal oil (lard oil) is taken in the market that is already available. Animal oil is made in four concentration series, namely 25ppm, 50ppm, 75ppm, and 100ppm and control (0ppm). The toxicity test was carried out in vitro using the toxic bait method, which was carried out by growing Nila pathogenic fungi inoculum on PDA media that had been mixed with animal oil. The results of animal fungicide toxicity testing in 5 treatments (A= control – E= 100 ppm) obtained the diameter of pathogenic fungal colonies (5 pathogenic fungi) ranging from 2.88 cm – 6.82 cm. Animal oil fungicides can affect pathogenic fungi in fish, this can be seen in pathogenic fungi Aspergillus sp. which has a relative resistance of 49% (100 ppm), the category is quite influential with a colony diameter of 3.5 cm and in the pathogenic fungus Mucor sp. With a relative resistance of 56% (100 ppm), the category is quite influential with a colony diameter of 2.88 cm.
Inventarization and Diversity Index of Gastropods in Meras Beach, Bunaken District, North Sulawesi Muhammad Rifki; Adelfia Papu; Saroyo Saroyo; Hizkia Rengkung; Ermas Isnaeni Lukman
JURNAL BIOS LOGOS Vol. 15 No. 2 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i2.64378

Abstract

Gastropods play an important role in maintaining the balance of coastal ecosystems, particularly as detritivores that support nutrient cycling. Pantai Meras, located in North Sulawesi, has a complex coastal ecosystem that includes mangrove forests, seagrass beds, and coral reefs, which support high biodiversity. However, information regarding gastropod diversity in this area remains limited. This study aims to identify gastropod species and analyze their diversity levels in the waters of Pantai Meras. A purposive sampling method was used for data collection, employing 0.5 m × 0.5 m plots placed randomly at 45 sampling points across three research stations with different substrate types, namely sandy substrate (Station 1), muddy sand substrate (Station 2), and sand mixed with coral fragments (Station 3). Data were analyzed using PAST software version 4.17 to calculate the Shannon diversity index (H’). The results showed a total of 243 gastropod individuals belonging to 89 species from six orders, with Neogastropoda being the dominant order, comprising 59 species. The most abundant species was Nassarius globosus, with a total of 26 individuals recorded. The overall Shannon diversity index (H') was 4.14, which falls into the high diversity category. The highest H' value was recorded at Station 1 (4.08), followed by Station 3 (4.02), and the lowest at Station 2 (3.43). The high gastropod diversity at Pantai Meras indicates that the coastal ecosystem in this area is still in good condition and capable of supporting sustainable gastropod populations. Environmental factors such as temperature, salinity, as well as the presence of mangroves, seagrass beds, and coral reefs contribute to the high species diversity of gastropods in the study area.

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