cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota adm. jakarta pusat,
Dki jakarta
INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
Arjuna Subject : -
Articles 35 Documents
 1   2   3   4 
Search results for , issue "Vol. 7 No. 1 (2020): June 2020" : 35 Documents clear
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Mufida, Diana Chusna; Salsabila, Yuna Annisa; Suswati, Enny; Hermansyah, Bagus; Agustina, Dini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
PURIFIKASI PROTEIN IMUNOGENIK 31 DAN 56 kDa DARI KELENJAR SALIVA Aedes aegypti Wathon, Syubbanul; Mutiah, Fitria; Oktarianti, Rike; Senjarini, Kartika
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9682.432 KB) | DOI: 10.29122/jbbi.v7i1.3931

Abstract

Purification of 31 and 56 kDa Immunogenic Proteins from the Salivary Glands of Aedes aegyptiThe salivary gland of arthropod vector contains various bioactive compounds and plays a role in the transmission of pathogens to the host. The host develops anti-salivary antibodies against vector saliva exposure. Our previous research has identified two immunogenic proteins with molecular weights of 31 and 56 kDa from the Aedes aegypti salivary gland protein extract. However, the role of the 31 and 56 kDa immunogenic proteins from saliva Ae. aegypti is not fully known, so it is necessary to purify two immunogenic protein fractions to better specify the target of developing a dengue vaccine. This study aimed to purify the 31 and 56 kDa immunogenic protein fractions by electroelution and dialysis methods. The purification of the two protein fractions has been successful which were confirmed by the SDS-PAGE by the existence of single-band parallel to the positive control. These results were further supported by the dot blot analysis which showed a positive reaction in the form of dark spots in the two protein fractions which were reacted with dengue patients' serum, endemic healthy people, and neonates. These results indicated that the purified 31 and 56 kDa immunogenic protein fraction can be identified by specific antibodies.Keywords: dialysis, electroelution, immunogenic, purification, saliva  ABSTRAKKelenjar saliva vektor arthropoda mengandung berbagai senyawa bioaktif dan berperan dalam transmisi patogen ke tubuh inang. Tubuh inang mengembangkan antibodi anti-saliva terhadap paparan saliva vektor. Penelitian kami sebelumnya telah mengidentifikasi dua protein imunogenik dengan berat molekul 31 dan 56 kDa dari ekstrak protein kelenjar saliva Aedes aegypti. Namun demikian, peranan protein imunogenik 31 dan 56 kDa dari saliva Ae. aegypti belum diketahui sepenuhnya sehingga perlu dilakukan purifikasi dua fraksi protein imunogenik untuk lebih menspesifikkan target pengembangan vaksin dengue. Tujuan penelitian ini untuk melakukan purifikasi fraksi protein imunogenik 31 dan 56 kDa melalui metode elektroelusi dan dialisis. Keberhasilan purifikasi dua fraksi protein 31 dan 56 kDa terbukti dari hasil konfirmasi SDS-PAGE dengan terbentuknya pita tunggal sejajar dengan kontrol positif. Hasil tersebut diperkuat dengan analisis dot blot yang menunjukkan reaksi positif dengan munculnya noktah gelap pada dua fraksi protein tersebut ketika direaksikan dengan serum pasien DBD, penduduk sehat endemik dan neonatus. Hasil ini mengindikasikan bahwa fraksi protein imunogenik 31 dan 56 kDa hasil purifikasi dapat dikenali oleh antibodi spesifik.
PROFIL HEMATOLOGI TIKUS (Rattus norvegicus) GALUR SPRAGUE-DAWLEY JANTAN UMUR 7 DAN 10 MINGGU Rosidah, Idah; Ningsih, Sri; Renggani, Tiya Novita; Efendi, Julham; Agustini, Kurnia
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3568

Abstract

Hematology Profile of Sprague-Dawley Male Rats (Rattus norvegicus) Aged 7 and 10 WeeksCurrently, the normal hematology profile has not been yet available for outbred rats in Indonesia. Most of the researchers use the data of hematology profile from references outside Indonesia in which the breeding environment is different. This study aimed to obtain a hematology profile of Sprague-Dawley male rats aged 7 and 10 weeks. The animals were obtained from the Laboratory of Animal Experiment, NADFC. Blood was drawn from the retro-orbital sinus and then analyzed to obtain the hematology profile including erythrocytes, hemoglobin, hematocrit, MCV, MCH, MCHC, leukocytes. Statistically significant differences for erythrocytes, hemoglobin, MCV, MCHC, thrombocyte, and leukocytes parameters were observed between rats aged 7 and 10 weeks. However, there were no statistically significant differences observed on MCH, thrombocyte, and body weight parameters. Data presented here would be useful as a reference for hematology research as well as in evaluating alterations in blood parameters.Keywords: erythrocyte, hematology, leukocyte, Sprague-Dawley, thrombocyteABSTRAKSaat ini belum banyak tersedia informasi profil hematologi untuk hewan coba tikus yang dikembangbiakkan di Indonesia. Kebanyakan peneliti menggunakan data profil hematologi dari referensi luar Indonesia. Penelitian ini bertujuan untuk mendapatkan data hematologi tikus jantan usia 7 dan 10 minggu.  Hewan coba yang digunakan adalah tikus jantan galur Sprague-Dawley yang diperoleh dari Laboratorium Hewan Percobaan-BPOM. Sebanyak 60 ekor tikus jantan yang terdiri dari 30 ekor usia 7 minggu dan 30 ekor usia 10 minggu diambil darahnya melalui sinus orbitalis. Selanjutnya, dianalisa jumlah sel eritrosit, hemoglobin, hematokrit, MCV, MCH, MCHC, leukosit. Hasil analisis statistik menunjukkan terdapat perbedaan signifikan antara tikus jantan usia 7 dan 10 minggu untuk parameter jumlah eritrosit, hemoglobin, hematokrit, MCV, MCHC dan leukosit. Tetapi tidak terdapat perbedaan signifikan untuk jumlah MCH, trombosit dan berat badan tikus. Data yang diperoleh dapat digunakan sebagai referensi penelitian yang berhubungan erat dengan profil hematologi dan penyakit kelainan darah lainnya.
KEANEKARAGAMAN ENZIM INVERTASE, PENGEMBANGAN STRAIN UNGGUL DAN TEKNOLOGI PRODUKSINYA Sanjaya, Wilhelmus Terang Arga; Giyanto, .; Widyastuti, Rahayu; Santosa, Dwi Andreas
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1328.71 KB) | DOI: 10.29122/jbbi.v7i1.3705

Abstract

Invertase Diversity, Novel Strain and Production Technology DevelopmentInvertase or β-fructofuranosidase (E.C.3.2.1.2.6) have been a valuable enzyme in food industry, so that research about enhancing invertase activity on an industrial scale has been reported massively. So far, the production of the invertase enzyme is highly dependent on the potential activity of the invertase enzyme derived from microorganisms. The development of enzyme production technology is also a concern in invertase research to obtain an efficient and inexpensive production system. So far, various developments in enzyme production technology and enzyme utilization have been carried out, including various innovations in immobilizing and increasing the stabilization of the invertase enzyme during the production process. The development of superior strains and enzyme production technology continues amid the discovery of the latest approaches such as genetic engineering, protein modification, and nanotechnology. This paper aims to discuss invertase variation from various organisms, its correlation to novel microbial strain development to increase invertase production and invertase enzyme production technologies development including immobilization technology and stabilization of invertase enzymes.Keywords: β-fructofuranosidase, ezyme production, fermentation, immobilitation, invertase geneABSTRAKInvertase atau β-fructofuranosidase (E.C.3.2.1.2.6) merupakan enzim yang sangat penting dalam dunia industri pangan sehingga berbagai optimasi aktivitas enzim untuk produksi dalam skala industri terus dilakukan. Produksi enzim invertase hingga sejauh ini sangat bergantung oleh potensi aktivitas enzim invertase yang diambil dari mikroorganisme. Selain itu, pengembangan teknologi produksi juga menjadi perhatian dalam pengembangan produksi dan pemanfaatan enzim untuk mendapatkan sistem produksi yang efisien dan murah. Sejauh ini berbagai pengembangan teknologi produksi enzim dan pemanfaatan enzim telah dilakukan, diantaranya dengan berbagai inovasi dalam melakukan imobilisasi dan peningkatan stabilisasi enzim invertase selama proses produksi. Pengembangan strain unggul dan teknologi produksi enzim terus dilakukan di tengah ditemukannya pendekatan-pendekatan terkini seperti rekayasa genentika, modifikasi protein, dan teknologi nano. Artikel ini bertujuan untuk membahas variasi enzim invertase dari berbagai organisme, korelasi pengembangan strain mikrobia unggul terhadap peningkatan produksi enzim invertase, dan perkembangan teknologi produksi enzim invertase meliputi teknologi imobilisasi dan stabilisasi enzim invertase.
FITOREMEDIASI AIR LIMBAH LABORATORIUM ANALITIK UNIVERSITAS JEMBER DENGAN PEMANFAATAN TANAMAN ECENG GONDOK DAN LEMBANG Novita, Elida; Wahyuningsih, Sri; Jannah, Dwi Andriana Na'imatul; Pradana, Hendra Andiananta
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1467.991 KB) | DOI: 10.29122/jbbi.v7i1.3850

Abstract

Phytoremediation of Analytical Laboratory of Jember University Waste Water by The Use of Water Hyacinth and Cattail PlantsAnalytical laboratory waste water at Jember University has organic and inorganic materials which can be categorized as biodegradable or non-biodegradable wastes. This study focused on comparing the ability between water hyacinth (Eichhornia crassipes) and cattail (Typha angustifolia) plant in reducing the pollutants as a consideration in selecting plants for waste water treatment at Jember University. The stages in this research consisted of filtration using silica sand, adsorption using activated carbon and zeolites, and phytoremediation using water hyacinth and cattail plants. The phytoremediation treatment was carried out during 14 days with a density of 40 g L–1. Cattail plant treatment had a higher value of pollutant reduction efficiency in waste water compared to water hyacinth. The reduction efficiency parameters. namely turbidity, TSS, BOD, COD, and Cr, were 92.18, 84, 74, 64, and 49%, respectively. The results of this study provide an alternative treatment for laboratory waste water which has an environmentally friendly character at Jember University.Keywords: Chromium (Cr), Eichhornia crassipes, filtration and adsorption, Typha angustifolia, water qualityABSTRAKAir limbah laboratorium analitik di Universitas Jember mengandung bahan organik dan anorganik yang bersifat mudah diuraikan maupun toksik. Tujuan penelitian ini adalah membandingkan kemampuan reduksi polutan oleh eceng gondok (Eichhornia crassipes) dan lembang (Typha angustifolia) sebagai pertimbangan pemilihan tanaman untuk menangani air limbah laboratorium di lingkungan Universitas Jember. Tahapan penelitian terdiri atas filtrasi menggunakan pasir silika, adsorpsi menggunakan karbon aktif dan zeolit, serta fitoremediasi menggunakan eceng gondok dan lembang. Waktu tanaman eceng gondok dan lembang diinkubasi menggunakan teknik fitoremediasi selama 14 hari dengan densitas 40 g L–1. Hasil penelitian menunjukkan bahwa penanganan air limbah menggunakan lembang memiliki nilai efisiensi reduksi polutan pada air limbah lebih tinggi daripada eceng gondok. Nilai efisiensi reduksi tersebut berupa parameter kekeruhan, TSS, BOD, COD, dan Cr secara berurutan sebesar 92,18, 84, 74, 64, dan 49%. Hasil penelitian ini menjadi alternatif penanganan air limbah laboratorium yang ramah lingkungan.
UJI KEMAMPUAN DAYA SERAP HANJUANG (Cordyline fruticosa) SEBAGAI AGEN FITOREMEDIASI LOGAM Pb PADA MEDIA TANAH Hernahadini, Nelis; Muharram, Luthfia Hastiani; Istiqomah, Noviani Arifina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.462 KB) | DOI: 10.29122/jbbi.v7i1.3859

Abstract

Absorption Capability Test of Hanjuang (Cordyline fruticosa) as Fitoremediation Agent of Lead in Soil MediumLead is a heavy metal waste that is dangerous for the environment and health. The use of ornamental plants is an alternative in reducing heavy metal pollution. Hanjuang (Cordyline fruticosa) is an ornamental-plant phytoremediation agent that can absorb heavy metals especially lead (Pb) at a high concentration. This study aims to test the Pb absorption ability of Hanjuang plant. Hanjuang was planted in a medium containing Pb at the concentration of 50 mg kg–1 with variable planting time of 4, 6, 8, 10, and 12 weeks. The measurement of Hanjuang's absorption of Pb was carried out on the roots, stems, and leaves by the atomic absorption spectroscopy (AAS) method. The results showed that the highest absorption capacity of 63.4% occurred at 12 days planting time. Whereas the amount of Pb accumulation in each part of the plant, from the highest to the lowest concentration, was found in the roots, leaves, and stems, consecutively. The ability of the plant's absorption of Pb was reduced with increasing metal concentrations in the media.Keywords: Hanjuang, heavy metal, lead, phytoremediation, solid wasteABSTRAKTimbal menjadi salah satu limbah logam berat yang berbahaya untuk lingkungan dan kesehatan. Penggunaan tanaman hias menjadi alternatif dalam mengurangi pencemaran logam berat. Hanjuang (Cordyline fruticosa) merupakan tanaman hias agen fitoremediasi yang memiliki kemampuan menyerap logam berat khususnya timbal (Pb) yang cukup tinggi. Penelitian ini bertujuan melakukan uji daya serap tanaman hanjuang terhadap logam Pb. Hanjuang ditanam pada medium tanah berlogam Pb dengan kadar 50 mg kg–1 dengan variabel waktu penanaman selama 4, 6, 8, 10 dan 12 minggu. Pengukuran daya serap Hanjuang terhadap Pb dilakukan pada bagian akar, batang, dan daun dengan metode spektroskopi serapan atom (AAS). Hasil menunjukkan bahwa daya serap tertinggi terjadi pada waktu tanam 12 hari dengan kadar 63,4%. Sedangkan jumlah akumulasi tiap bagian tanaman paling tinggi ke rendah terdapat pada bagian akar, daun, dan batang. Pada variasi konsentrasi, kemampuan daya serap tanaman terhadap Pb berkurang seiring meningkatnya konsentrasi logam pada media.
BIOCHEMICAL CHARACTERIZATION OF RECOMBINANT PHYTASE ENZYME (phyK) FROM Klebsiella sp. ASR1 ENCAPSULATED WITH ALGINATE Muhammad Eka Hidayatullah; . Sajidan; Ari Susilowati; Baraka Stewart Mkumbe; Ralf Greiner
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (910.584 KB) | DOI: 10.29122/jbbi.v7i1.2997

Abstract

Karakterisasi Biokimia Enzim Fitase Rekombinan (phyK) dari Klebsiella sp. ASR1 Yang Dienkapsulasi Dengan AlginatEnzim fitase melepas molekul fosfor pada atom C dari benzena Inositol fitat. Tetapi fitase memiliki kelemahan tidak mampu bertahan terhadap kondisi ekstrim dalam lambung nonruminansia. Solusi dalam penelitian ini yaitu fitase dienkapsulasi menggunakan alginat. Penelitian ini bertujuan mengkarakterisasi fitase setelah dienkapsulasi menggunakan alginate. Hasil penelitian ini yaitu fitase yang dienkapsulasi memiliki aktivitas tertinggi pada pH 6,0, sedangkan fitase tanpa enkapsulasi pada pH 5,0. Suhu optimum untuk aktivitas tertinggi fitase yang dienkapsulasi yaitu 70ºC, sedangkan fitase tanpa enkapsulasi 37ºC. Untuk perlakuan penambahan ion logam, aktivitas tertinggi fitase yang dienkapsulasi terjadi dengan penambahan 0,1 mM Fe2+ dan 1,0 mM Ca2+, sedangkan fitase tanpa enkapsulasi dengan penambahan 0,1 mM Fe2+. Berdasarkan hasil penelitian ini, fitase yang dienkapsulasi memiliki keunggulan lebih banyak dibandingkan dengan fitase tanpa enkapsulasi, karena mampu bertahan pada pH dan suhu tinggi, dan beberapa efek ion logam.Kata Kunci: alginat, asam fitat, enkapsulasi, fitase, fitase rekombinanABSTRACTPhytase enzymes release phosphorus molecules on the C atom from benzene inositol phytate. But phytase has the disadvantage of being unable to withstand extreme conditions in the non-ruminant stomach. The solution in this research was phytase encapsulated using alginate. This study aims to characterize phytase after being encapsulated using alginate. The results of this study were the encapsulated phytase had the highest activity at pH 6.0, while the unencapsulated phytase at pH 5.0. The optimum temperature for the highest activity of the encapsulated phytase was 70ºC, while the unencapsulated phytase 37ºC. For treatment of metal ion addition, the highest activity of the encapsulated phytase occurred with the addition of 0.1 mM Fe2+ and 1.0 mM Ca2+, while the unencapsulated phytase with the addition of 0.1 mM Fe2+. Based on the results of this study, the encapsulated phytase had more advantages compared to the unencapsulated phytase, as the former withstand high pH and temperature, and some metal ion effects.
PERBANYAKAN AKASIA HIBRIDA (Acacia mangium × Acacia auriculiformis) MELALUI SUBKULTUR BERULANG Yelnititis Yelnititis; Sri Sunarti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (446.385 KB) | DOI: 10.29122/jbbi.v7i1.3113

Abstract

In vitro culture is a promising technique for mass propagation of high-value species. Study of propagation for Acacia hybrid (A. mangium x A. auriculiformis) through this technique has been conducted using single node stem from seedlings as explants. Growth medium used was modified Murashige and Skoog (MS), basal medium Woody Plant Medium (WPM), and Gamborg (B5) supplemented. The study was conducted in two stages, namely shoot induction and shoot multiplication. The treatment tested was the Benzyl Adenine (BA) supplementation at the concentration of 0.3; 0.7; and 1.0 mgL-1 of. Observation was conducted on the frequency of shoot induction, number of shoot, shoot length and visual performance of the culture. The result showed that treatment of BA 0.7 mgL-1 on modified MS medium is the best for shoot induction, shoot multiplication and visual performace of the culture. The average of number of shoot was 2.6; 5.0 and 7.7 shoots on the first three consecutive subcultures. Changing to different basal medium on the fourth subculture showed that the treatment of BA 0.7 mgl-1 is the best condition for shoot regeneration (12.60 shoots) and shoot length (6.97 cm). The culture from this treatment showed the best visual morphological performance.Keywords:Acacia hybrid; multiplication; subculture; in vitro; BA. ABSTRAKKultur in vitro merupakan suatu teknik yang menjanjikan untuk perbanyakan massal spesies-tanaman bernilai tinggi. Penelitian perbanyakan akasia hibrid (A. mangium x A. auriculiformis) melalui kulturin vitro telah dilakukan dengan menggunakan eksplan berupa batang satu buku yang berasal dari anakan. Media tumbuh yang digunakan adalah media dasar Murashige dan Skoog (MS) yang sudah dimodifikasi, media dasar Woody Plant Medium (WPM), dan Gamborg (B5). Penelitian dilakukan dalam dua tahap yaitu induksi tunas dan perbanyakan tunas. Perlakuan yang diuji adalah penggunaan Benzyl Adenine (BA) dengan konsentrasi 0,3; 0,7 dan 1,0 mg L-1. Pengamatan dilakukan terhadap waktu induksi tunas, jumlah tunas, tinggi tunas dan penampilan biakan secara visual. Hasil penelitian menunjukkan bahwa penggunaan BA 0,7 mg L-1 pada media MS modifikasi merupakan perlakuan terbaik untuk induksi tunas, perbanyakan tunas, tinggi tunas, dan kondisi biakan secara visual. Jumlah rata-rata tunas yang dihasilkan dari perlakuan ini adalah 2,6; 5,0 dan 7,7 tunas pada subkultur pertama, kedua dan ketiga. Pada penggunaan media dasar berbeda pada subkultur keempat menunjukkan bahwa perlakuan BA 0,7 mg L-1 merupakan perlakuan terbaik dengan jumlah tunas sebanyak 12,60 tunas dan rata-rata tinggi tunas 6,97 cm. Biakan yang dihasilkan dari perlakuan tersebut mempunyai penampilan yang baik dan normal.
PENENTUAN JENIS KELAMIN BURUNG KENARI (Serinus canaria) BERDASARKAN GEN Chromodomain Helicase DNA-Binding 1 (CHD1) Afif Muhammad Akhrom; Indarjulianto Soedarmanto; Yanuartono Yanuartono; Trini Susmiati; Alfarisa Nururrozi; Slamet Raharjo
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.004 KB) | DOI: 10.29122/jbbi.v7i1.3178

Abstract

Phenotype determination of sex in young canaries is very low in accuracy. This study aimed to develop a genotypic sexing method in canaries. This study used 12 canaries consisting of 3 mature males, 3 mature females and 6 one-month-old canaries. Phenotypic sexing by cloacal observation was done on all birds, continued by genotypic sexing to identification CHD1 gene using polymerase chain reaction (PCR). The PCR used blood samples for mature canaries, and feather for mature and one-month-old canaries. The results of phenotypic observations showed that all mature male canaries had prominent and pointed cloaca forms, all mature females had flat and wide, whereas all one-month-old birds had a flat cloaca. The result of PCR showed a single band (500 bp) for mature male and double bands (500 bp and 300 bp) for mature female canaries. The PCR results of one-month-old canaries showed that there were one male and five females. Based on this study, it was concluded that genotypic sexing using the PCR method is effective in the sex determination of canaries.Keywords: canary, CHD1, genotype, PCR, sexing ABSTRAKPenentuan jenis kelamin burung kenari muda secara fenotip akurasinya sangat rendah. Penelitian ini bertujuan untuk menentukan jenis kelamin burung kenari secara genotip. Penelitian ini menggunakan 12 ekor burung kenari, terdiri dari 6 ekor dewasa (3 jantan, 3 betina) serta 6 ekor umur 1 bulan. Semua burung ditentukan jenis kelaminnya dengan mengamati kloaka dan identifikasi gen CHD1 menggunakan teknik polymerase chain reaction (PCR). Sampel DNA berasal dari darah dan bulu untuk burung dewasa serta bulu untuk burung umur 1 bulan. Pengamatan fenotip menunjukkan bahwa burung kenari dewasa jantan mempunyai bentuk kloaka menonjol dan runcing, dewasa betina berbentuk datar dan lebar, sedangkan semua burung umur 1 bulan mempunya bentuk kloaka datar. Hasil identifikasi gen CHD1 diperoleh adanya 1 pita gen sekitar 500 bp dari sampel darah dan bulu semua burung kenari dewasa jantan, dan 2 pita gen sekitar 500 bp dan 300 bp dari sampel semua burung kenari betina dewasa. Hasil PCR pada sampel burung umur 1 bulan menunjukkan bahwa 1 ekor jantan dan 5 ekor betina. Berdasarkan penelitian ini dapat disimpulkan bahwa penentuan jenis kelamin secara genotip menggunakan gen CHD1 dapat dilakukan pada burung kenari.
PROFIL HEMATOLOGI TIKUS (Rattus norvegicus) GALUR SPRAGUE-DAWLEY JANTAN UMUR 7 DAN 10 MINGGU Idah Rosidah; Sri Ningsih; Tiya Novita Renggani; Julham Efendi; Kurnia Agustini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3568

Abstract

Hematology Profile of Sprague-Dawley Male Rats (Rattus norvegicus) Aged 7 and 10 WeeksCurrently, the normal hematology profile has not been yet available for outbred rats in Indonesia. Most of the researchers use the data of hematology profile from references outside Indonesia in which the breeding environment is different. This study aimed to obtain a hematology profile of Sprague-Dawley male rats aged 7 and 10 weeks. The animals were obtained from the Laboratory of Animal Experiment, NADFC. Blood was drawn from the retro-orbital sinus and then analyzed to obtain the hematology profile including erythrocytes, hemoglobin, hematocrit, MCV, MCH, MCHC, leukocytes. Statistically significant differences for erythrocytes, hemoglobin, MCV, MCHC, thrombocyte, and leukocytes parameters were observed between rats aged 7 and 10 weeks. However, there were no statistically significant differences observed on MCH, thrombocyte, and body weight parameters. Data presented here would be useful as a reference for hematology research as well as in evaluating alterations in blood parameters.Keywords: erythrocyte, hematology, leukocyte, Sprague-Dawley, thrombocyteABSTRAKSaat ini belum banyak tersedia informasi profil hematologi untuk hewan coba tikus yang dikembangbiakkan di Indonesia. Kebanyakan peneliti menggunakan data profil hematologi dari referensi luar Indonesia. Penelitian ini bertujuan untuk mendapatkan data hematologi tikus jantan usia 7 dan 10 minggu.  Hewan coba yang digunakan adalah tikus jantan galur Sprague-Dawley yang diperoleh dari Laboratorium Hewan Percobaan-BPOM. Sebanyak 60 ekor tikus jantan yang terdiri dari 30 ekor usia 7 minggu dan 30 ekor usia 10 minggu diambil darahnya melalui sinus orbitalis. Selanjutnya, dianalisa jumlah sel eritrosit, hemoglobin, hematokrit, MCV, MCH, MCHC, leukosit. Hasil analisis statistik menunjukkan terdapat perbedaan signifikan antara tikus jantan usia 7 dan 10 minggu untuk parameter jumlah eritrosit, hemoglobin, hematokrit, MCV, MCHC dan leukosit. Tetapi tidak terdapat perbedaan signifikan untuk jumlah MCH, trombosit dan berat badan tikus. Data yang diperoleh dapat digunakan sebagai referensi penelitian yang berhubungan erat dengan profil hematologi dan penyakit kelainan darah lainnya.

Page 2 of 4 | Total Record : 35

 1   2   3   4 

Filter by Year

2020 2020


Reset
Filter By Issues
All Issue Vol. 10 No. 1 (2023): Juni 2023 Vol. 9 No. 2 (2022): December 2022 Vol. 9 No. 1 (2022): June 2022 Vol. 8 No. 2 (2021): December 2021 Vol. 8 No. 1 (2021): June 2021 Vol. 7 No. 2 (2020): December 2020 Vol. 7 No. 1 (2020): June 2020 Vol. 6 No. 2 (2019): December 2019 Vol. 6 No. 1 (2019): June 2019 Vol 6, No 1 (2019): June 2019 Vol. 5 No. 2 (2018): December 2018 Vol 5, No 2 (2018): December 2018 Vol. 5 No. 1 (2018): June 2018 Vol 5, No 1 (2018): June 2018 Vol. 4 No. 2 (2017): December 2017 Vol 4, No 2 (2017): December 2017 Vol 4, No 1 (2017): June 2017 Vol. 4 No. 1 (2017): June 2017 Vol. 3 No. 1 (2016): June 20160 Vol 3, No 1 (2016): June 20160 Vol. 3 No. 2 (2016): December 2016 Vol 3, No 2 (2016): December 2016 Vol. 3 No. 1 (2016): June 2016 Vol 3, No 1 (2016): June 2016 Vol. 2 No. 2 (2015): December 20150 Vol 2, No 2 (2015): December 20150 Vol 2, No 1 (2015): June 20150 Vol. 2 No. 1 (2015): June 20150 Vol 2, No 2 (2015): December 2015 Vol. 2 No. 2 (2015): December 2015 Vol 2, No 1 (2015): June 2015 Vol. 2 No. 1 (2015): June 2015 Vol. 1 No. 1 (2014): December 20140 Vol 1, No 1 (2014): December 20140 Vol. 1 No. 1 (2014): December 2014 Vol 1, No 1 (2014): December 2014 More Issue