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All Journal Bioscience Jurnal Pengajaran MIPA Biogenesis: Jurnal Ilmiah Biologi BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pendidikan IPA (JPPIPA) BIOEDUKASI Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology BIOEDUSCIENCE Jurnal Pengabdian Masyarakat AbdiMas Indonesian Journal of Biotechnology and Biodiversity Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Jurnal Kreativitas PKM Manuju : Malahayati Nursing Journal Jurnal Dedikasi Pendidikan Poltekita : Jurnal Ilmu Kesehatan Journal of Appropriate Technology for Community Services Jurnal Abdi Insani Jurnal Marketing Nusantara Science and Technology Proceedings Jurnal Pengabdian UNDIKMA Jurnal Pengabdian Nasional (JPN) Indonesia Jurnal Pengabdian kepada Masyarakat JURNAL STUDIA KOMUNIKA KATALIS: Jurnal Penelitian Kimia dan Pendidikan Kimia DIKSAINS : Jurnal Ilmiah Pendidikan Sains Jurnal Locus Penelitian dan Pengabdian Muhafadzah: Jurnal Ilmiah Bimbingan dan Konseling Islam RANGGUK: Jurnal Pengabdian Kepada Masyarakat
Seprianto, Seprianto
Program Studi Bioteknologi, Fakultas Ilmu-ilmu Kesehatan, Universitas Esa Unggul Jl. Arjuna Utara No. 09 Kebun Jeruk, Jakarta 11510
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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ISOLASI DAN PENAPISAN BAKTERI SELULOLITIK DARI BERBAGAI JENIS TANAH SEBAGAI PENGHASIL ENZIM SELULASE Seprianto, Seprianto
Indonesian Journal of Biotechnology and Biodiversity Vol 1, No 2 (2017): Indonesian Journal of Biotechnology and Biodiversity
Publisher : Indonesian Journal of Biotechnology and Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

AbstractCellulose is an enzyme that hydrolyzes β-1,4-glycosidic bonds on cellulose molecules to produce glucose. The utilization of cellulase enzymes is currently increasing in the food, paper , detergent and agricultural industries. This research was aimed at obtaining cellulolytic bacterial isolates capable of producing cellulose enzyme where enzyme activity was measured daily by DNS method and to add bacterial isolate collection which will be used for further research. Screening of cellulolytic bacteria from soil led to detection or one potential isolate, designated as 6.2 showed highest activity among others which occurred on the 11th day with activity value of 0,0189 μmol/minute and the lowest activity of cellulase enzyme was not observed its activity, while the highest dissolved protein content occurred on the sixth day with activity of 0,408240784 mg/ml and the lowest protein content on day 2th of 0,167673552 mg / ml. The highest activity of specific enzyme occurred on the 11th day with a value of 0,0538 U/mg, while the lowest activity of specific enzyme was not activity with value of 0 U/mg. Keywords : screening, cellulolytic bacteria, cellulose AbstrakEnzim selulase merupakan enzim yang dapat menghidrolisis ikatan β-1,4-glikosidik pada molekul selulosa sehingga menghasilkan glukosa. Pemanfaatan enzim selulase saat ini semakin meningkat dalam dunia industri makanan, industri kertas, industri detergen dan industri pertanian. Penelitian ini bertujuan untuk mendapatkan spesies bakteri selulolitik yang potensial menghasilkan enzim selulase dan melihat aktivitas enzim dengan metode pengukuran DNS serta menambah koleksi isolat bakteri dari biakan murni yang akan digunakan untuk penelitian selanjutnya. Dari hasil penelitian penapisan bakteri selulolitik yang diisolasi dari berbagai jenis tanah didapatkan satu isolat yang potensial yaitu Isolat 6.2 menunjukan aktivitas lebih baik diantara yang lainnya dengan aktivitas enzim ektrak kasar tertinggi terjadi pada hari ke-11dengan nilai 0,0189 µmol/menit dan aktivitas enzim selulase terendah tidak teramati aktivitas enzimnya atau sama dengan nilai 0 µmol/menit, sedangkan kadar protein terlarut tertinggi terjadi pada hari ke-6 dengan nilai 0,408240784 mg/ml dan kadar protein terendah pada hari ke-2 sebesar 0,167673552 mg/ml. Aktivitas enzim spesifik tertinggi terjadi pada hari ke-11 dengan nilai 0,0538 U/mg, sedangkan aktivitas enzim spesifik terendah tidak memiliki aktivitas atausama dengan nilai 0 U/mg. Kata kunci : penapisan, bakteri selulolitik, enzim selulase
PRESERVICE CHEMISTRY TEACHERS’ CHEMICAL LITERACY BASED ON PISA AND TIMSS RESULTS FOR INTERNATIONAL AND INDONESIAN STUDENTS Seprianto, Seprianto; Jofrishal, Jofrishal; Mauliza, Mauliza
Jurnal Pengajaran MIPA Vol 22, No 2 (2017): JPMIPA: Volume 22, Issue 2, 2017
Publisher : Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18269/jpmipa.v22i2.8647

Abstract

PISA and TIMSS results have been used as a basis for educational reform, but unfortunately, teachers’ understanding of PISA and TIMSS tests is rarely investigated. We evaluated 149 sophomore preservice chemistry teachers’ achievement in TIMSS and PISA-like test. As a way to reflect on TIMSS and PISA results, we compared preservice chemistry teachers’ achievement to PISA and TIMSS results for Indonesia and the international students. Results suggested that, to a certain degree, preservice chemistry teachers have a better chemical literacy than Indonesian students and a somewhat similar chemical literacy with international students.
KNOWLEDGE INVENTORY OF ETHNOCYMIA BASED ON ACEH LOCAL WISDOM FOR CHEMICAL LECTURE DEVELOPMENT Seprianto, Seprianto; Jofrishal, Jofrishal
Jurnal Pengajaran MIPA Vol 24, No 1 (2019): Jurnal Pengajaran MIPA - April 2019
Publisher : Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18269/jpmipa.v24i1.13569

Abstract

Ethnochemistry is a chemical applied by certain cultural groups. This study aims to obtain information and descriptions of what ethnochemistry practiced by the Acehnese tribe in East Aceh and what potential of ethnochemistry can be developed in chemistry lectures. This research was carried out in East Aceh Regency and Langsa City. The research subjects consisted of teachers, lecturers, administrators of the Majelis Adat Aceh (MAA) and community leaders from East Aceh who were well acquainted with ethnochemical practices in the community. This research is a qualitative research model with ethnographic methods. The stages of the study included the theoretical study stage, namely literacy studies on ethnography of Acehnese tribal communities and applied chemistry and the stages of empirical study or data collection to inventory the ethnochemical knowledge practiced by the Acehnese people. Data collection was carried out using observation techniques, documentary studies, and interviews with research instruments include observation sheets, documentation, and interview guidelines. Data analysis techniques in research use a qualitative approach. Data obtained from interviews, observations, and documentation are analyzed simultaneously by first sorting out similar data (categorization) and then doing data reduction, presentation, and conclusions as well as verification. The results of the study showed that the ethnochemical knowledge of the tribes of Aceh in East Aceh that could be developed in chemistry lectures included the analysis of chemical studies on: natural ingredients used as medicines; natural ingredients used as food additives; natural ingredients used as beauty and cleaning tools and materials; appropriate technology; and processing typical Acehnese food.  ABSTRAKEtnokimia adalah kimia yang diterapkan oleh kelompok budaya tertentu. Penelitian ini bertujuan untuk memperoleh informasi dan deskripsi tentang etnokimia apa saja yang dipraktikkan suku Aceh di Aceh Timur dan potensi apa saja dari etnokimia tersebut yang dapat dikembangkan dalam perkuliahan kimia. Penelitian ini dilaksanakan di Kabupaten Aceh Timur dan Kota Langsa. Subjek penelitian terdiri dari guru, dosen, pengurus Majelis Adat Aceh (MAA) serta tokoh masyarakat asal Aceh Timur yang mengenal betul praktik etnokimia di masyarakat. Penelitian ini merupakan model penelitian kualitatif dengan metode etnografi. Tahapan penelitian meliputi tahap kajian teori yaitu studi literasi tentang etnografi masyarakat suku Aceh dan terapan ilmu kimia serta tahap kajian empirik atau pengumpulan data untuk menginventarisasi pengetahuan etnokimia yang dipraktikkan masyarakat Aceh. Pengumpulan data dilakukan dengan menggunakan teknik pengamatan, studi dokumenter, dan wawancara dengan instrumen penelitian meliputi lembar observasi, dokumentasi dan pedoman wawancara. Teknik analisis data dalam penelitian menggunakan pendekatan kualitatif. Data yang diperoleh dari wawancara, pengamatan, dan dokumentasi dilakukan analisis secara simultan dengan terlebih dahulu melakukan pemilahan data yang sejenis (kategorisasi) selanjutnya dilakukan reduksi data, penyajian, dan kesimpulan serta verifikasi. Hasil penelitian menunjukkan pengetahuan etnokimia suku Aceh di Aceh Timur yang dapat dikembangkan dalam perkuliahan kimia meliputi analisis kajian kimia tentang: bahan alam yang dijadikan obat-obatan; bahan alam yang dijadikan zat aditif makanan; bahan alam yang dijadikan alat dan bahan kecantikan serta pembersih; teknologi tepat guna; dan pengolahan makanan khas Aceh. Keywords: Aceh, ethnochemistry, inventory, local wisdom, etnokimia, inventarisasi, kearifan lokal.
PENINGKATAN PENGETAHUAN MASYARAKAT TENTANG TANAMAN TRANSGENIK MELALUI EDUKASI ONLINE Wahyuni, Febriana Dwi; Novianti, Titta; Saraswati, Henny; Seprianto, Seprianto
Jurnal Pengabdian Masyarakat AbdiMas Vol 8, No 01 (2021): Jurnal Pengabdian Masyarakat Abdimas
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/abd.v8i01.4785

Abstract

Transgenic plants are genetically modified plants made by inserting one or a number of genes from other organisms, with the aim of obtaining superior and desirable new traits, such as resistance to drought stress, resistance to pests, resistance to herbicides. So far, there are still many people who are worried about the safety of transgenic plants that have been successfully marketed. Therefore, we strive to provide information based on existing references to increase public understanding of GM crops. The educational materials provided consisted of an introduction to plant breeding techniques and the purpose of genetic transformation in plants, gene cloning techniques and the transformation of target genes into plant genomes to produce transgenic plants and molecular techniques used to select transgenic plants which were delivered in the form of online seminars. via zoom and live streaming on youtube. The flow of activities is divided into two stages, namely material presentation and discussion. This activity is expected to increase knowledge about assembling techniques for transgenic plants as an effort to educate the public regarding the safety of transgenic plants. Keywords: biotechnology, cloning, GMOs, transgenic plants
PENGENALAN BIOTEKNOLOGI DAN METODE KULTUR JARINGAN SEBAGAI UPAYA PENINGKATAN WAWASAN SISWA DI SMA YAYASAN PERSIAPAN GENERASI BARU Wahyuni, Febriana Dwi; Novianti, Titta; Saraswati, Henny; Seprianto, Seprianto
Jurnal Pengabdian Masyarakat AbdiMas Vol 6, No 3 (2020): Jurnal Pengabdian Masyarakat Abdimas
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/abd.v6i3.3333

Abstract

AbstractHigh school students is a level of secondary school education that will enter a higher level of education in tertiary institutions. Of course, a lot of preparation must be done as a provision after graduating, both knowledge and skills. This community service activity is one of the first steps to introduce and enhance secondary school students' insights about biotechnology. Biotechnology is a relatively new study program in Indonesia. Biotechnology has many focus areas that can be studied, including tissue culture and microbiology. The community service activity aims to provide knowledge not only to students, but also to teachers in the Senior High School Foundation Preparation Foundation regarding tissue culture and microbiology techniques. The method used for the delivery of the material is the lecture and practicum methods. Mastery of tissue culture and microbiology is expected to make students as creative individuals in creating a product that is related to these two focus areas. Keywords: biotechnology, tissue culture, microbiology AbstrakSiswa SMA merupakan tingkatan dari pendidikan sekolah menengah yang akan memasuki tingkatan pendidikan yang lebih tinggi di perguruan tinggi. Tentunya banyak persiapan yang harus dilakukan sebagai bekal setelah lulus nantinya, baik ilmu maupun keterampilan. Kegiatan pengabdian kepada masyarakat ini merupakan  salah satu langkah awal untuk memperkenalkan dan meningkatkan wawasan siswa Sekolah Menengah tentang bioteknologi. Bioteknologi merupakan salah satu program studi yang terhitung baru di Indonesia. Bioteknologi mempunyai banyak bidang fokus yang bisa dipelajari, diantaranya yaitu kultur jaringan dan mikrobiologi. Adapun kegiatan pengabdian kepada masyarakat ini bertujuan untuk memberikan pengetahuan tidak hanya kepada siswa, tetapi juga kepada guru di lingkungan SMA Yayasan Persiapan Generasi Baru mengenai teknik-tekniik kultur jaringan dan mikrobiologi. Adapun metode yang digunakan untuk penyampaian materi tersebut adalah metode ceramah dan praktikum. Penguasaan tentang kultur jaringan dan mikrobiologi ini diharapkan dapat menjadikan siswa sebagai individu yang kreatif dalam menciptakan suatu produk yang berkaitan dengan kedua bidang fokus tersebut. Kata Kunci : bioteknologi, kultur jaringan, mikrobiologi
PKM PENINGKATAN MUTU PANGAN LOKAL BERBASIS PANGAN FERMENTASI DI PULAU PAYUNG, KEPULAUAN SERIBU Seprianto, Seprianto; wahyuni, Febriana Dwi
Jurnal Pengabdian Masyarakat AbdiMas Vol 6, No 2 (2020): JURNAL PENGABDIAN MASYARAKAT ABDIMAS
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/abd.v6i2.3186

Abstract

AbstractThe Thousand Islands Region is administratively included in DKI Jakarta Province but has different characteristics from other DKI Jakarta areas. Payung Island is one of the islands in the Thousand Islands which is a marine tourism destination that is quite crowded with tourists. The diversity of processed products, especially fish-based products, is still little developed on this island, so there are not many choices for tourists. The purpose of this activity is to develop diversified food products by improving the quality of fermented-based local food. Through the cooperation of UMKMBarakuda Payung Island partners, the implementation of community service is going well. The method of implementation is by presentation and direct practice such as making peda fish, while other processed products such as bekasang and fish sauce were only a video documentary. A total of 13 members of the Barakuda group participated in this training. Processed products from fish have not been developed much by UMKM Barakuda, Payung Island. This activity also collaborates with the Product Design Team, so that it can provide an overview of the product from manufacturing to the packaging stage. It is expected that the implementation of this activity will increase the role of the community to be creative, innovative, independent, and entrepreneurial so that they can actively participate in regional and national development and become an attraction for tourists to visit the Thousand Islands. Keywords :Payung island, local food, fermentation food AbstrakWilayah Kepulauan Seribu secara administratif masuk ke dalam Propinsi DKI Jakarta, namun memiliki  karakteristik yang berbeda dengan wilayah DKI Jakarta lainnya. Pulau Payung merupakan salah satu pulau di Kepulauan Seribu yang menjadi destinasi wisata bahari yang cukup ramai dikunjungi oleh wisatawan.Keberagaman produk olahan terutama berbahan dasar ikan masih sedikit dikembangkan di pulau ini, sehingga tidak banyak pilihan bagi wisatawan.Tujuan kegiatan ini adalah untuk pengembangan diversifikasi produk pangan dengan meningkatkan mutu pangan lokal berbasis fermentasi.Melalui kerjasama mitra UMKM Barakuda Pulau Payung, pelaksanaan pengabdian masyarakat ini berjalan dengan baik.Metode pelaksanaannya dengan presentasi dan praktek langsung seperti pembuatan ikan peda, sedangkan produk olahan lainnya seperti bekasang dan kecap ikan hanya berupa video dukumenter.Sebanyak 13 anggota kelompok UMKM Barakuda mengikuti pelatihan ini.Produk olahan dari ikan tersebut belum banyak dikembangankan oleh UMKM Barakuda Pulau Payung.Kegiatan ini juga berkalaborasi dengan Tim Desain Produk, sehingga dapat memberikan gambaran produk mulai dari pembuatan sampai pada tahap pengemasannya. Diharapkan dari pelaksanaan kegiatan ini akan meningkatan peranan masyarakat untuk kreatif, inovatif, mandiri, serta  berwirausaha sehingga mampu berpartisipasi aktif dalam pembangunan daerah dan nasional dan menjadi daya tarik wisatawan untuk berkunjung ke Kepulauan Seribu. Kata kunci:Pulau payung, pangan lokal, pangan fermentasi
ISOLASI DAN PENAPISAN BAKTERI SELULOLITIK DARI BERBAGAI JENIS TANAH SEBAGAI PENGHASIL ENZIM SELULASE Seprianto, Seprianto
Indonesian Journal of Biotechnology and Biodiversity Vol 1, No 2 (2017): Indonesian Journal of Biotechnology and Biodiversity
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

AbstractCellulose is an enzyme that hydrolyzes β-1,4-glycosidic bonds on cellulose molecules to produce glucose. The utilization of cellulase enzymes is currently increasing in the food, paper , detergent and agricultural industries. This research was aimed at obtaining cellulolytic bacterial isolates capable of producing cellulose enzyme where enzyme activity was measured daily by DNS method and to add bacterial isolate collection which will be used for further research. Screening of cellulolytic bacteria from soil led to detection or one potential isolate, designated as 6.2 showed highest activity among others which occurred on the 11th day with activity value of 0,0189 μmol/minute and the lowest activity of cellulase enzyme was not observed its activity, while the highest dissolved protein content occurred on the sixth day with activity of 0,408240784 mg/ml and the lowest protein content on day 2th of 0,167673552 mg / ml. The highest activity of specific enzyme occurred on the 11th day with a value of 0,0538 U/mg, while the lowest activity of specific enzyme was not activity with value of 0 U/mg. Keywords : screening, cellulolytic bacteria, cellulose AbstrakEnzim selulase merupakan enzim yang dapat menghidrolisis ikatan β-1,4-glikosidik pada molekul selulosa sehingga menghasilkan glukosa. Pemanfaatan enzim selulase saat ini semakin meningkat dalam dunia industri makanan, industri kertas, industri detergen dan industri pertanian. Penelitian ini bertujuan untuk mendapatkan spesies bakteri selulolitik yang potensial menghasilkan enzim selulase dan melihat aktivitas enzim dengan metode pengukuran DNS serta menambah koleksi isolat bakteri dari biakan murni yang akan digunakan untuk penelitian selanjutnya. Dari hasil penelitian penapisan bakteri selulolitik yang diisolasi dari berbagai jenis tanah didapatkan satu isolat yang potensial yaitu Isolat 6.2 menunjukan aktivitas lebih baik diantara yang lainnya dengan aktivitas enzim ektrak kasar tertinggi terjadi pada hari ke-11dengan nilai 0,0189 µmol/menit dan aktivitas enzim selulase terendah tidak teramati aktivitas enzimnya atau sama dengan nilai 0 µmol/menit, sedangkan kadar protein terlarut tertinggi terjadi pada hari ke-6 dengan nilai 0,408240784 mg/ml dan kadar protein terendah pada hari ke-2 sebesar 0,167673552 mg/ml. Aktivitas enzim spesifik tertinggi terjadi pada hari ke-11 dengan nilai 0,0538 U/mg, sedangkan aktivitas enzim spesifik terendah tidak memiliki aktivitas atausama dengan nilai 0 U/mg. Kata kunci : penapisan, bakteri selulolitik, enzim selulase
Isolasi dan Identifikasi Bakteri Probiotik Dari Usus Udang Windu (Penaeus monodon) Berdasarkan Sekuens Gen 16S rDNA Seprianto S; Feliatra F; Titania T Nugroho
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 2 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i2.3943

Abstract

Probiotics are living microorganisms that have beneficial effect on the host by improving the balance of bacteria in the intestines. This research was aimed to determine the diversity of bacteria that live in digestion of the shrimp and molecular characteristics along with to observe phylogenetic relationships among the bacteria identified based on sequence16S rDNA. The bacteria were isolated from intestine of the Tiger prawn (Penaeus monodon) obtained from fishpond at BBPBAP Jepara. The analyzed result of 16S rDNA shown that three bacterial species were potential as probiotic. These bacteria grow well at pH 2 and this indicates one of probiotic bacteria characteristic. Among the three isolates, two isolates i.e. SP2 and SU isolates were phylogenetically closely related to Bacillus bataviensis strain CCGE2059 (EU867382.1) with 97% homology. While the SWU Isolate is most likely a new species of Caulobacter sp. (AJ227775.1). The SWU isolates were phylogenetically one ancestor with the Chromobacterium violaceum.
Candida antarctica Lipase B Synthetic Gene: A Bioinformatics Analysis Febriana Dwi Wahyuni; seprianto Seprianto
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.901 KB) | DOI: 10.24036/0201822100216-0-00

Abstract

Enzyme exploration is important to support the development of biotechnology. To facilitate microbial discovery, it can be done synthetically using bioinformatics methods. Candida antarctica lipase B (CALB) is one of enzyme derived from microorganisms and has been applied as a biocatalyst in several industry. The methods used in this study are the analysis of gene structure using NCBI, analysis of protein CALB sequence using Uniprot, analysis of 3D protein structure using Swiss model, analysis primary design using primer3 and analysis of restriction sites using snapgene. The construction of synthetic calB gene obtained based on the results of the CalB gene sequence in geneBank using NCBI with access number Z30645.1. CalB gene wildtype is modified by adding several restriction enzyme (XhoI, XbaI, ClaI, and BglI), 6 Histags, and 2x stops codon and produce 1083 base pairs. CALB protein has 342 amino acids. Based on 3D structure, CALB protein has three molecules in common with one homotrimer ring that will encircle the double helix DNA. Primers used for calB gene amplification are CALB forward 5'-TCCCCAGTATCAGGTCCAAG-3 ' and CALB reverse 5'-GACACCTGAGGCTGAACGAT-3'.
Cloning of a Transglutaminase Gene from Streptomyces thioluteus TTA 02 SDS 14 Seprianto Seprianto; Dewi Seswita Zilda; Yusro Nuri Fawzya; Suharsono Suharsono; Puspita Lisdiyanti; Agustinus Robert Uria
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 1 (2016): May 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i1.189

Abstract

Microbial Transglutaminase (MTGase, EC 2.3.2.13) is an enzyme that catalyzes the transfer of acyl group. Many microbial strains that produce MTGase belong to Streptomyces members. This research was aimed at cloning of a MTGase gene. PCR–based screening of ten MTGase-producing streptomyces isolates from soil in West Nusa Tenggara led to detection of one potential isolate, designated as TTA 02 SDS 14. The partial  MTGase-encoding gene (470 bp)  was amplified by PCR and sequenced. The sequence result indicate its similarity of 93 % with that of Streptomyces cinnamoneus. The 16S rRNA gene analysis showed its identity as Streptomyces thioleteus. Fosmid-based construction of a genomic library from the isolate  and subsequent screening led to the isolation of  a ~40-Kb fosmid harboring a MTGase gene.
Co-Authors Adelia, Ismi Adri Nora Agustinus Robert Uria alfero Putra Iryanto Alham, Fiddini Anisa Febriyanti Ariyadi, Noprizal Aroem Naroeni Ayu, Yulia Agustina Azwar, Beni callista marsya Chikita, Daien Cyndi Prasetya Daflaini, Daflaini Darwata, Siti Riva DEWI PURNAMA Dewi Seswita Zilda Dewi Seswita Zilda Dewi, Lisa Rahma Dewi, Mustika Sandra Dina Hajja Ristianti, Dina Hajja Erani, Friska Fadila Fadila, Fadila Fajariyanto, Gunawan Febriana Dwi Wahyuni Febriana Dwi Wahyuni feby feby Feliatra Gloria, Meilina Gusvina, Fadilla Hana Nurullita Prestisia Harna, Harna Hartini Henny Saraswati Henny Saraswati Idi Warsah Irfandi, Ahmad Jofrishal Jofrishal Juniyati, Sintia Kevin Febrianus Moda Kibtiyah, Maryatul Lawrence, Yoel Lesmawan, Hendra Mahyuny, Siska Rita Manalu, Mely Grace Manalu Maulida, Maulida Mauliza, Mauliza Muhamad Amza Sidiq Muhammad Arreza nie nie Noer Khasanah Noperta, Noperta Novianti, Titta Nugraha, Jaka Setia Nurmalasari, Mieke Nursanah, Nursanah Ogi Danika Pranata Oksal, Efriyana Oktaviani Naulita Turnip Oktaviani, Coryna Puspita Lisdiyanti PUSPITA LISDIYANTI PUTRI HANDAYANI Putri Handayani putri mega utami Putri, Mentari Darma Rahmah, Rasya Aulia Rahmayanti R, Andi Riansyah, Irza Rini Hidayati roaslein putri Roaslein Putri Roselein Putri Ruhmayanti, Nur Ayu Sa'pang, Mertien sabar pambudi Saraswati, Henny Saraswati, Henny Seda, Ananda Putri Sianturi, Dody Jhonatan Wilfiez Sidauruk, Christina Siti Komariyah Situmorang, Larissa Kristina T.T. Soleha, Sovatunisa Suharsono Suharsono Surgasari, Tania Susanti, Sela Syamsul Rizal Titania Tjandrawati Nugroho Veza Azteria Widodo Widodo Wiriatmaja, Nuraini Ulya Yusro Nuri Fawzya Yusro Nuri Fawzya Zadrax, Adellino Zhafira, Alipya