cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Ricko Dharmadi Utama
Contact Email
apinaga53@gmail.com
Phone
+6282256602225
Journal Mail Official
editormltj@gmail.com
Editorial Address
Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714
Location
Kota banjarmasin,
Kalimantan selatan
INDONESIA
Medical Laboratory Technology Journal
Published by Poltekkes Kemenkes Banjarmasin
ISSN : -     EISSN : 24610879     DOI : https://doi.org/10.31964/mltj
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 8 Documents
 1 
Search results for , issue "Vol. 10 No. 1 (2024): June" : 8 Documents clear
Rosmarinus officinalis and Centella asiatica Affect Interleukin-6 Zebrafish Larvae Induced High Glucose Prastiwi, Fajar Dwi; Lamadi, Sutini; Khotimah, Husnul; Wiyasa, I Wayan Arsana; Raras, Tri Yudani M.
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.538

Abstract

Gestational Diabetes Mellitus (GDM) is diabetes that occurs during pregnancy. GDM is characterized by hyperglycemia. Hyperglycemia causes an inflammatory response characterized by the excretion of pro-inflammatory cytokines, one of which is IL-6. Centella asiatica and Rosmarinus officinalis are known as anti-diabetic and anti-inflammatory. This study aims to study the effect of the combination of nanoemulsion Centella asiatica and Rosmarinus officinalis on the expression of Interleukin-6 in Zebrafish larvae induced by 3% glucose. Hyperglycemia is known from increased levels of Phosphoenolpyruvate carboxykinase in Zebrafish larvae. Phosphoenolpyruvate carboxykinase and Interleukin-6 expression were measured by conventional PCR method. The combination of Rosmarinus officinalis nanoemulsion and Centella asiatica was given with three different doses, namely at a concentration of 2.5, 5 and 10 µg/mL. The statistical analysis used was One Way ANOVA to measure Interleukin-6 expression levels. The results of this study prove that the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions reduces the expression of Interleukin-6. The dose closest to the negative control was at a concentration of 5 μg/mL, although the final result was statistically significant (p<0.05). In conclusion, combining nanoemulsion Centella asiatica and Rosmarinus officinalis with the right dose reduced levels of Interleukin 6 in zebrafish larvae induced by high glucose.
Analyze of Parameter Values of Sasirangan Home Industry Wastewater at Hot and Cold Dyeing Noraida, Noraida; Khair, Abdul
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.555

Abstract

The sasirangan fabric home industry produces wastewater through the hot and cold dyeing process. It is necessary to know the coloring process and wastewater parameter values to design good wastewater treatment. The purpose of the study was to compare the wastewater parameter values including pH, NH3-N, BOD5, COD, TSS, Oil/Fat, Color, Phenol, Temperature, H2S and Crom Total from Sasirangan Home Industry Wastewater at Hot and Cold Dyeing. This type of research is comparative research with static group comparison design. The sample is a portion of wastewater from home industries from in the province of South Kalimantan Indonesia, namely Banjarmasin City, Banjarbaru City, and Banjar Regency. The sampling technique used accidental sampling with a total of 20 samples consisting of 10 samples of the hot dyeing process and 10 samples of the cold dyeing process. Parameters that do not meet the standards in the hot dyeing process are pH, BOD5, COD, TSS, color, and phenol, while in the cold dyeing process are BOD5, COD, TSS, and color. Statistically, there is a significant difference in the parameter values of pH, TSS, color, phenol, and temperature in sasirangan cloth home industry wastewater between the two processes. The results of the research can be used for planning wastewater treatment for the Sasirangan home industry.
Formulation Test of Dracontomelon dao (Blanco) Merr. & Rofe Leaf Extract Powder as an Anti-Diarrheal Escherichia coli Method In Vivo Dwiyanti, Ratih Dewi; Nurlailah, Nurlailah; Thuraidah, Anny
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.556

Abstract

Dracontomelon dao (Blanco) Merr. & Rofe is one of the medicinal plants used in the community. This study aims to analyze the effectiveness of  Dracontomelon dao (Blanco) Merr. & Rofe extract powder as an antidiarrheal against Escherichia coli in vivo. Dracontomelon dao (Blanco) Merr. & Rofe leaf comes from Hulu Sungai Utara, South Kalimantan, Indonesia, and is macerated with 96% ethanol—Dracontomelon dao extract powder (Blanco) Merr. & Rofe is made with three parts thick extract, four parts Lactose, and four parts Starch. Experimental rat were conditioned to develop diarrhea and were treated with extract powder with varying doses per kg of body weight, namely 250 mg, 500 mg, and 750 mg. After treatment on day 6, rat recovered from diarrhea in the 750 mg dose treatment group as much as 78%, while the positive control total recovery on day 6 reached 100%. After treatment on day 6, the number of Escherichia coli colonies in rat feces decreased, namely the lowest in the 750mg dose treatment group, 276 colonies/gr. This research concludes that Dracontomelo dao (Blanco) Merr. & Rofe leaf extract powder is effective as an anti-diarrhea against Escherichia coli in experimental rat. Further research is needed on human test subjects to obtain anti-diarrhea drugs with safer and more effective formulations.
The Effect of Methanol Fractıonated of Luvunga sarmentosa on In Vıtro Sperm Membrane Integrıty Permatasari, Silvani; Nuha, Alya Firyal; Ratnasari, Arini; Widayati, Ratna; Ka Praja, Rian
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.571

Abstract

Infertility cases in the world are increasing in 2020, with 186 million individuals affected worldwide. The most common cause of male infertility is due to decreased sperm quality. Sperm membrane integrity is known to play an essential role in the fertilization ability of spermatozoa. The methanol fraction of Luvunga sarmentosa root extract has the highest antioxidant activity compared to other fractions and can improve sperm motility in vitro. This study aims to determine the effect of the methanol fraction of Luvunga sarmentosa root extract on human sperm membrane integrity and the effective concentration of Luvunga sarmentosa methanol extract fraction to improve sperm membrane integrity in vitro. The samples used were washed human spermatozoa in vitro, then added BWW medium, and incubated at 37℃ for 1 hour with methanol fraction of Luvunga sarmentosa root extract at concentrations of 100, 500, 1000, and 5000ng/ml as well as the control group (BWW only). Furthermore, 100uL of each sample was added to 1mL of Hypo-osmotic swelling (HOS) solution, then incubated at 37oC for 30 minutes to see the integrity of the spermatozoa membrane from the tail swelling. The results showed that sperm membrane integrity significantly increased at 100, 500, 1000, and 5000ng/mL concentrations compared to the control group. The methanol fraction of Luvunga sarmentosa root extract was most effective at a concentration of 100ng/mL. Methanol fraction of Luvunga sarmentosa root extract can improve human sperm membrane integrity.
Increased levels of IL-4 in the Spleen of BALB/c Mice after 65.5 kDa Pili Protein Klebsiella pneumoniae Immunization Putra Uta, Diego Rafi; Mufida, Diana Chusna; Sutejo, Ika Rahmawati; Agustina, Dini; Suswati, Enny; Shodikin, Muhammad Ali
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.575

Abstract

Pneumonia is an infection of the lung parenchyma caused by Klebsiella pneumoniae, resulting in a high mortality rate of millions each year. To reduce these deaths, one potential solution is to create a vaccine that utilizes virulence factors of this microorganism, such as pili. During the early phase of infection, these antigens have a crucial role and can stimulate the production of memory b cells. These cells are activated by IL-4 (interleukin-4) in lymphoid organs, such as the spleen. Pathogen exposure such as virulence factors can stimulate the secretion of IL-4 in the spleen organ. This study aims to investigate the role of Klebsiella pneumoniae pili in this process. The research conducted from May to December 2023 at the Microbiology Laboratory, Faculty of Medicine, University of Jember was purely experimental, using Balb/c mice with IL-4 levels in the spleen organ as variables. The study used 15 mice, divided into control (K1), adjuvant (K2), and antigen (K3) groups. The research data were analyzed using non-parametric tests, specifically the Kruskal-Wallis and Post Hoc tests. The Kruskal-Wallis test revealed significant differences (p=0.003). In the Post Hoc test, a significant difference was found between the control and antigen groups (p=0.002). The results conclusively demonstrate that induction of Klebsiella pneumoniae pili protein 65.5 kDa significantly increases IL-4 levels in the spleen. Future studies should consider adding serum specimens to provide additional information.
The Impact of Phaleria macrocarpa Fruit Flavonoid Extract on Endometrial Thickness in Mice Menopausal Model Wijaya, Dyah Ayu Septika; Dwi Wulandari, Recavery; Sutrisno, Sutrisno; Irwanto, Yahya; Anita, Kenty Wantri
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.584

Abstract

This study aims to determine how the effect of flavonoid phaleria macrocarpa fruit extract on the endometrial thickness of Mus musculus which is used as an experimental animal with a menopause model. This study employed a randomized post-test-only control group design on 32 female mice. Ovariectomy simulated menopause, followed by treatment with flavonoid from Phaleria macrocarpa fruit extract. Post-observariectomized, follicle-stimulating hormone levels indicated menopausal status. Hematoxylin-Eosin methods assessed endometrial thickness. The result indicated reduced endometrial thinning in the positive control group (K+). Conversely, the treatment group (P1-P4) exhibited increased endometrial thickness post-flavonoid extract administration. Ovariectomized-induced estrogen decline resulted in reduced endometrial thinning, mimicking menopausal conditions. Statistically, flavonoid extract administration increased endometrial thickness in ovariectomized-induced menopausal mice, with optimal effect observed at 7.5 mg/mice/day for 14 days. The preferred intervention dose for altering endometrial thickness in menopausal conditions was identified as P1 (3.75 mg/mice/day). In conclusion, a flavonoid from Phaleria macrocarpa fruit extract mitigates endometrial thinning in the menopausal mice model. For implication in menopausal women, clinical trials need to be conducted to evaluate the dosage of these flavonoids for their potential as a natural SERM (Selective Estrogen Receptor Modulator) before clinical application in managing endometrial health. Additionally, research on molecular effects particularly regarding ER (Estrogen Receptors) in the endometrial organ, is needed to confirm their phytoestrogenic effect.
Negative Effect of Cigarette Smoke: Black Garlic Opportunities for Prevention of Ovulation Disorders Asih, Sari Wulan; Nurseta, Tatit; Handono, Kusworini; Yudhanto, Hendy Setyo
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.585

Abstract

Women's issues are primarily related to infertility. This study aimed to demonstrate that ovarian healing in wistar rats exposed to cigarette smoke was impacted by the administration of extracted black garlic. This research uses True Experimental methods in vivo in the laboratory to identify the cause and effect of the variables being tested. This method involves replication, randomization, and control. The design chosen was a post-test-only control group design, where the experimental group received treatment and the control group did not, without random selection. The results of the study showed that there was no effect due to exposure to cigarette smoke with an increase in cortisol and a decrease in the number of secondary follicles in the ovaries of female wistar rats as well as the administration of Black Garlic (Allium sativum) extract. Black garlic extract unaffected cortisol levels and the number of secondary follicles or repair of the ovaries due to exposure to cigarette smoke. Further research is needed in dosing black garlic extract or combining it with other ingredients to provide effective results.
Extract Ethanol of Tempuyung (Sonchus arvensis) Leaves as Anti-Hyperuricemia: In Vitro Studies Parisa, Nita; Hidayat, Rachmat; Fatmawati, Fatmawati
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.594

Abstract

Hyperuricemia is a condition where there is an increase in blood uric acid levels above 7 mg/dL in men and 6 mg/dl in women. One of the enzymes that plays a role in hyperuricemia is xanthine oxidase which can inhibit uric acid synthesis. Tempuyung leaves (Sonchus avernis) are one of the plants that have antihyperuricemia properties. This study aims to determine the effect of ethanol extract of tempuyung leaves on xanthine oxidase in vitro. Tempuyung leaves come from Palembang, South Sumatra, Indonesia, and are macerated with 96% ethanol until a thick extract is obtained. The xanthine oxidase inhibition test was carried out on tempuyung leaf extract and Allopurinol with respective concentrations of 6.25; 12.5; 25; and 50 ppm and followed by absorption measurements using UV-V spectrophotometry at a wavelength of 293 nm and determining the IC50 value. The research results showed that the ethanol extract of daun tempuyung leaves had an IC50 value of 23.37 ppm, higher than allopurinol of 17.16 ppm which was used as a reference. These results classify the ethanol extract of daun tempuyung leaves as a strong inhibitor of the xanthin oxidase enzyme. Therefore, the ethanol extract of daun tempuyung leaves has the potential to be anti hyperuricemic.

Page 1 of 1 | Total Record : 8

 1 

Filter by Year

2024 2024


Reset
Filter By Issues
All Issue Vol. 11 No. 2 (2025): December Vol. 11 No. 1 (2025): June Vol. 10 No. 2 (2024): December Vol. 10 No. 1 (2024): June Vol. 9 No. 2 (2023): December Vol. 9 No. 1 (2023): June Vol. 8 No. 2 (2022): December Vol. 8 No. 1 (2022): June Vol. 7 No. 2 (2021): December Vol. 7 No. 1 (2021): June Vol. 6 No. 2 (2020): December Vol. 6 No. 1 (2020): June Vol. 5 No. 2 (2019): December Vol. 5 No. 1 (2019): June Vol. 4 No. 2 (2018): December Vol. 4 No. 1 (2018): June Vol. 3 No. 2 (2017): December Vol. 3 No. 1 (2017): June Vol. 2 No. 2 (2016): December Vol. 2 No. 1 (2016): June Vol. 1 No. 2 (2015): December Vol. 1 No. 1 (2015): June More Issue