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Articles 482 Documents
Effect of Calina Papaya Leaves Extract on Respiratory Tract in Cigarette Smoke Exposed Rats Haris Setiawan; Arlinda Sekar Ayu Hikmawati; Sri Wijayanti Wulandari; Eriza Via Malinda; Ichsan Luqmana Indra Putra
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.19068

Abstract

Cigarette smoke is a source of free radicals that cause health problems throughout the world. Indonesia is a country that has many sources of natural antioxidants in counteracting free radicals, one of which is Calina papaya leaves. The study aimed to determine the potential of Calina papaya leaves antioxidants in improving the respiratory organs of Wistar rats after being exposed to cigarette smoke. The study using 25 male Wistar rats consisted of Control (not exposed to cigarette smoke and given distilled water), KN (cigarette smoke and given distilled water), P1 (cigarette smoke and Calina Papaya Leaves Ethanolic extracts (CPLE) 100 mg/kg BW), P2 (cigarette smoke and CPLE 200 mg/kg BW), and P3 (cigarette smoke and given CPLE 300 mg/kg BW). Exposure to cigarette smoke and administration of extracts was carried out for 21 days. On day 22, the rats were euthanized and dissected to remove the lungs and trachea. Organs were prepared using the paraffin method and hematoxylin-eosin staining. Parameters consisted of histopathological observations of the lungs and trachea. All data parameters were analyzed using a one-way ANOVA test and Duncan's advanced test (p<0.05). The results showed that there was an improvement in histopathology of the lungs and trachea at a dose of 200 mg/Kg BW compared to other doses (p<0.05). The study showed that the Calina papaya leaves ethanolic extracts have the potential as a source of antioxidants in improving the respiratory organs of Wistar rats after being exposed to cigarette smoke.
The Epilithic Diatom Community as a Bioindicator of water quality Brangkal Subwatershed in the Mojokerto Area Bayu Agung Prahardika; Widi Muhammad Lutsfi Dwi Styawan
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.20439

Abstract

Diatoms are widely used as bioindicators of water quality because of their short life cycle and sensitivity to changes of environmental conditions in waters.  One type of diatom that is used as a bioindicator is an epilithic diatom. Epilithic diatoms are one of the diatom microalgae communities attached to rock, so they are difficult to be carried away by currents. This study aimed to determine the type, composition, abundance, diversity, and dominance of epilithic diatoms found in the waters of the Brangkal subwatershed, and to determine the quality of water in the Brangkal subwatershed based on the epilithic diatom tropic index. Epilithic diatom samples were taken from 4 stations in the Brangkal subwatershed, Mojokerto Regency at the beginning of the dry season (July-August). The analysis carried out includes abundance, diversity index, dominance index, and Diatom Trophic Index (TDI). Based on the results of the study, 17 genera of epilithic diatoms were found, with the highest abundance of epilithic diatoms, including Navicula, Cocconeis, Nitszchia, and Gomphonema. The diversity index of all stations was in the medium category, with a value between 1.206 to 1.882. The lowest dominance index at station 1 was 0.167 and the highest was at station 4 with 0.373. In addition, the observed water quality is based on the Diatom Trophic Index (TDI), stations 1 to 3 are in a good category, and stations 4 are in a bad category. 
Association Analysis of Seagrass Coverage and Human Activities in Nusa Lembongan Suko Wardono; Elland Yupa Sobhytta; I Gusti Ngurah Agung Dhananjaya; Rodo Lasniroha; Yuniarti Karina Pumpun; Mochammad Miftakhul Mashuda; Dewa Gde Tri Bodhi Saputra; Permana Yudiarso
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.20307

Abstract

Nusa Lembongan has high marine biodiversity, including seagrass. Seagrass is a plant that lives submerged in a marine or estuary water that functions as a nursery ground, trapping sediment, and beach protector, so it is important to know the condition of seagrass coverage, especially in Nusa Lembongan for managing the Nusa Penida Marine Protected Area. This study aimed to understand the condition of seagrass coverage and the factors influencing the existence of its ecosystem in Nusa Lembongan. According to reslut in two stations, it was found that six of the twelve types of seagrasses in Indonesia, namely Enhalus acoroides, Thalassia hemprichii, Cymodocea serrulata, Cymodocea rotundata, Halodule pinifolia, and Halophila ovalis. From the two stations (LMB01 and LMB02), the total seagrass coverage was 38.10±30.98% or the medium category. The seagrass communities in the station areas were generally formed by 3 types of seagrasses; Thalassia hemprichii, Cymodocea serrulata, and Cymodocea rotundata. LMB02 has higher seagrass coverage than LMB01. The seagrass coverage is inversely proportional to the intensity of human activity.
Spawning and Reproductive Potential of Blue Swimming Crab (Portunus pelagicus) at Spermonde Archipelago, Indonesia Muh Saleh Nurdin; Amanda Pricella Putri; Dewi Yanuarita Satari; Riris Yuli Valentine; Fauziah Azmi; Teuku Fadlon Haser
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.19893

Abstract

The problem in the management of blue swimming crab (BSC) fisheries is a decrease in stock that overlaps with an increase in fishing activities. This study aimed to analyze the ratio of spawning potential (SPR), reproductive potential, and reproductive productivity of BSCs taken from the Spermonde Islands. Spawning potential was analyzed using SPR, while reproductive potential analysis used the relative proportion of data between female, berried female and the average fecundity of each class size. The results showed that the BSC in Spermonde were growth overfishing and recruitment overfishing. The SPR of the BSC was estimated to be 7%, below the biological reference point. The highest reproductive potential index of berried females occurred in the group with a carapace width of 111-120 mm which contributed 36.84% of the total egg production. The value of reproductive productivity was 1.35 indicating a productive population. Current legislation allows the capture of BSCs with carapace sizes larger than 100 mm. Based on the data of this study, this size limit has the potential to eliminate 65.92% of the total egg production in the Spermonde Islands. To ensure the sustainability of BSCs in the Spermonde Islands, it is necessary to limit size by capturing BSCs >120 mm to protect the berried female and increase total egg production.
Allopurinol Induction on Histopathological Structure of the Liver in Male Mice (Mus musculus) Dian Fita Lestari; Fatimatuzzahra Fatimatuzzahra; Agnes Petra Sianipar; Shahnaz Shabrina Wulansari
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.18616

Abstract

Allopurinol is used to reduce total uric acid levels in the body into oxypurinol which can inhibit xanthine oxidase. Allopurinol inhibits the precursors of uric acid formation, xanthine, and hypoxanthine. However, consumption of the drugs can cause side effects on the liver. The aim of the research was to determine the effect of allopurinol induction on the liver histopathology of male mice (Mus musculus) DDY strain. The method used in this research was an experimental design used post-test only that was divided into 4 groups of 4 mice per group. The control group (P0) was given 0.5% Na-CMC, and groups I, II, and III (P1, P2, and P3) were induced by allopurinol at 10 mg/kg BW, 20 mg/kg BW, and 30 mg/kg BW for 14 days. Allopurinol induction was performed by oral gavage. The results of the research showed that treatment with allopurinol caused changes in the mice’s body weight, liver index, liver morphology, and histological structure of the liver tissue, including necrosis, steatosis, leukocyte infiltration, binuclear hepatocytes, hepatocyte swelling, congestion, sinusoid dilatation, and hemorrhage. The level of liver damage increased in line with the dose used. This research indicated that the higher the allopurinol level, the higher the level of alteration in the liver section structure. Long-term use of allopurinol can cause damage to the structure of mice liver (liver toxicity). 
Feeding Behavior and Preferences of Hemiplecta humphreysiana and Lissachatina fulica (Gastropoda) to Support Heliciculture in Indonesia Jessima Pratiwi; Fuji Anandi; Pamungkas Rizki Ferdian; Tri Hadi Handayani; Raden Lia Rahadian Amalia; Narti Fitriana; Ayu Savitri Nurinsiyah
Jurnal Biodjati Vol 7, No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.19725

Abstract

The trend of Heliciculture for human consumption, animal feed, medicine, and neutricosmeceutical is rising globally. To gain a better knowledge on the Heliciculture of Indonesian land snails, we studied the daily activity with a focus on feeding behavior and the feed preference of two land snail species Hemiplecta humphreysiana (HH) and Lissachatina fulica (LF). The two species were selected because of their abundancein nature, as well as to compare behavior and preference between native (HH) and invasive (LF) species. Three days of daily activity observation was conducted by using a scan animal sampling method with 5 minutes intervals towards three individuals of HH and LF. The experiment on feeding preference was conducted towards ten individuals per species in 28 days of observation (14 days each for vegetables and fruits). Each individual was placed in different rearing boxes. A multiple-choice test method was applied and consisted of two treatments i.e. vegetable treatment (pakcoy, chicory, lettuce, cabbage) and fruit treatments (mango, cucumber, melon, papaya). The nutritional value from the observed feed was also analyzed. Based on the total of 51,840 minutes of observation records, both species were generally active from 21.00-03.00. HH was more active in moving when given vegetable treatment. The species preferred to feed on cucumber, melon, lettuce, and pakcoy. LF was more active in moving and feeding when treated with fruits. The species is more adaptive when given new feed and likes all four fruits, but preferred lettuce in the vegetable treatment. Both species prefer to feed with relatively high protein, high fiber, and moderate total carbohydrate content. Providing the right feed and at the right time can support the effectiveness of Heliciculture.
In Silico Analysis of Catechin, Galangin, and Hesperidin as Competitors of the SARS-CoV-2 Spike Protein Astuti Kusumorini; Nisrina Hasna; Yani Suryani; Opik Taufiqurrahman4
Jurnal Biodjati Vol 8, No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.24521

Abstract

Currently, Covid-19 has become endemic. However, the development of Covid-19 drugs continues to be carried out to suppress the growth of the Sars-Cov-2 virus. Some compounds with antiviral activity are catechin, galangin, and hesperidin. Angiotensin-converting enzyme-2 (ACE-2) is a protein that enters viruses into the cell. Based on that, ACE-2 can be used as a primary target to suppress the development of the Sars-Cov-2 virus. This study aimed to test the catechin, galangin, and hesperidin compounds in inhibiting the SARS CoV-2 virus from attaching to ACE-2 by trying the interactions of catechin, galangin, and hesperidin compounds with ACE-2 using the in-silico method. The material used was the three-dimensional structure of the compounds catechin, galangin hesperidin, and ACE-2. The tools used were FAF-Drugs4, Discovery Studio, and Pyrex software. Low-affinity energy values (kcal/mol) indicate promising results. The results showed that the energy affinity value of catechin was -6.2 kcal/mol, galangin was -6.3 kcal/mol, and hesperidin was -8.3 kcal/mol. This value is lower than the control affinity energy (chloroquine and favipiravir), which is  -5.2 kcal/mol and -4.8 kcal/mol, respectively. Based on this, catechin, galangin, and hesperidin can be used as inhibitors/competitors for the Sars-Cov-2 to attach to ACE-2.
Genetic Characteristics of Chloropsis cochinchinensis Gmelin, 1789 Based on The Mitochondrial DNA COI Gene Jarulis Jarulis; Apin Saputra; Risky Hadi Wibowo
Jurnal Biodjati Vol 8, No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.20260

Abstract

The rate of illegal poaching of blue-winged leafbirds (Chloropsis cochinchinensis) throughout Indonesia, particularly Bengkulu, is quite high. However, only minimal molecular information is available for this species. We performed mtDNA COI gene sequencing to explore genetic characters (conservative site, variable site, parsimony site, and singleton site) of blue-winged leafbirds. Using Qiagen's DNeasy® Blood and Tissue Kit based on the Spin-Column Protocol, total DNA was isolated, and PCR amplification methods were performed. DNA derived from a PCR reaction was forwarded to PT. First Base Malaysia for sequencing. Using MEGA 10.0 and BIOEDIT, the COI gene nucleotide sequence data were assembled, edited, and analyzed to explore of single nucleotide polymorphism, genetic distance, and phylogeny. The 616 bp COI genes contained 566 conservative sites (C), 50 variation sites (V), 24 information parsimony sites (Pi), and 26 singleton sites (S), as indicated by the results. The greatest nucleotide base composition was cytosine (34.1–34.9%), while the lowest was guanine (15.7–16.2%). The proportion of adenine-thymine nucleotide base pairs was greater than that of guanine-cytosine (50.3%). There were 26 barcode-specific mutation sites, 17 transition substitution mutation sites, and 9 transverse substitution mutation sites. The average genetic distance between C. cochinchinensis individuals was 2.2%, but the average genetic difference between species was 9.0%. All C. cochinchinensis individuals in our sample clustered within the same clade and were distinguished from other species within the same genus. The COI gene sequences of C. cochinchinensis that we acquired are novel and can be utilized for molecular identification of the species.
Vegetation Structure and Composition of Coffee Agroforestry in Kalibaru District Titanio Auditya Pribadi; Mufidah Afiyanti; Luchman Hakim
Jurnal Biodjati Vol 8, No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.23826

Abstract

Agroforestry is a form of sustainable agriculture; the present threat of agroforestry is a change in agroforestry landforms that have switched to a less sustainable form. Coffee plays a role in the agroforestry ecosystem, Kalibaru is one of the Banyuwangi District coffee centers managed by the local community. The objective of this study was to determine the vegetation structure and analyze the effect of that on environmental parameters. Data was collected by vegetation analysis and soil parameter analysis with PAST 2020 Software version 4.05. In total, 15 research plots were used, consisting of 5 complex agroforestry, 5 simple agroforestry, and 5 monoculture plots. The results showed 20 species in complex agroforestry, 24 species in simple agroforestry, and 18 species in monoculture  were found. The highest important index level in complex agroforestry: Durio zibethinus 63.87%  for trees, the Swietenia mahagoni 64.43%  for poles, and Coffee canephora 128.87% for shrubs. In simple agroforestry : Cocos nucifera 72.83% for trees, Leucaena leucocephala  80.70%  for poles, and the Coffea canephora 137.57%  for shrubs. In monoculture agroforestry: Hibiscus similis 82.99%  for trees, Leucaena leucocephala 209.22% for poles, and Coffea canephora 130.96% for shrubs. Agroforestry in Kalibaru was categorized  in the moderate diversity. The diversity index (H') in complex agroforestry showed H' trees 2.21, pole 2.03 and. shrubs 1.60. Simple agroforestry shows H' tree 2.00, pole 2.49, and shrubs 1.50. Monoculture shows H' tree 1.98, pole 0.64, and shrubs 1.54. Agroforestry a role in maintaining soil moisture and temperature, complex agroforestry: temperature 24 0C, and Moist 5.82, simple: temperature 24.2 0C, Moist 5.42, and Monoculture temperature 26 0C, Moist 4.54.
Diversity and Epidermal Characteristic of Lauraceae Leaf in Two Forest Locations, Bogor Regency, West Java Nurul amalia fadhila; Sulistijorini Sulistijorini; Nina Ratna Djuita
Jurnal Biodjati Vol 8, No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.24406

Abstract

Lauraceae is a group of tropical plants with a wide distribution. The abundance and species richness of the Lauraceae family includes the under to the upper canopy of the forest. The Lauraceae family can be utilized in various sectors, especially economically. Limiting characters as a significance in the Lauraceae classification was needed to provide additional information regarding taxon grouping. This research aimed to study epidermal characteristics, leaf venation variations, and the diversity of Lauraceae species under different forest environmental conditions. The research procedure involved: collecting ecological field data and morphological identification using a purposive sampling method based on the presence of species. Soil sampling and environmental microclimate were measured on each plot. The anatomical process was carried out by recording characters. Correlation between environmental factors and plant targets used canonical correspondence analysis. The kinship between members of Lauraceae was expressed in a dendrogram. The result of this study showed that Lauraceae could live at various elevations. The analysis data indicated differences in environmental factors that affect the existence of Lauraceae species. Leaf venation studies support clustering in the family using characters such as primary, secondary, tertiary veins, and areola. Observation of leaf venation can be used as a taxonomic significance in the family Lauraceae. Epidermal cell walls in leaf samples are straight, curved shallow, deep, and sharp. Other characters that can be found are anomocytic, laterocytic, and brachyparasitic types of stomata, with stellate and glandless trichomes The anatomical dendrogram separated Lauraceae into clusters on a similarity coefficient scale.