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BIOEDUSCIENCE

bioeduscience
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Published by Universitas Muhammadiyah Prof. Dr. Hamka

ISSN : 2614154X EISSN : 26141558 DOI : -

Core Subject : Science, Education, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education

BIOEDUSCIENCE is an open access journal that publishes research in the field of Biology and Biosain Education such as: Applied and implemented in education and learning, Botany, Zoology, Microbiology, Ecology, Biotechnology, Molecular Biology, Genetics, Bioinformatics, Cell and Developmental Biology, Biodiversity and Bioconservation. BIOEDUSCIENCE is published by Biology Education Study Program, Universitas Muhammadiyah Prof. Dr. Hamka, Indonesia regularly in June and December. ISSN : 2614-1558; P-ISSN : 2614-154X.

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Articles 544 Documents

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Hubungan Motivasi, Kelelahan, dan Beban Kerja Terhadap Kinerja Perawat di Ruang Rawat Inap Rumah Sakit Ibu dan Anak (RSIA) Bunda Jakarta Tahun 2019 Pradnya Paramita; Dewi Sri Rahayuningsih
BIOEDUSCIENCE Vol 6 No 3 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: Based on performance appraisal data at RSIA Bunda Jakarta in 2016 shows that the percentage of nurses who have poor performance is 124 people (55.6%) and those who have good performance are 99 people (44.4%). And in 2017, it showed that the percentage of nurses who had poor performance was 64 people (36.2%), and those who had good performance were 113 people (63.8%). This study aimed to determine the relationship between motivation, fatigue, and workload on nurse performance in the Mother and Child Hospital Bunda Jakarta in February 2019. Method: Quantitative analytical research using a cross-sectional approach. The sample in this study was 72 nurses in the inpatient ward of Mother and Child Hospital Jakarta. Result: The bivariate results showed that there was a significant relationship between motivation (p 0.001), fatigue (p 0.038), and workload (p 0.001). Multivariate results showed that motivation and workload were the most dominant variables related to nursing performance (P<0.005). The workload variable is the most dominant variable with the most considerable Exp (β) of 5.625. Conclusion: Two variables were significantly (dominantly) related to nursing performance: motivation and workload.

Faktor-Faktor yang Berhubungan Antara Pemberi Pelayanan Kesehatan Gigi Terhadap Pasien Disaat Kejadian Coronavirus Disease 2019 (Covid-19) Pada Praktek Klinik Pradnya Paramita; Nuraini
BIOEDUSCIENCE Vol 6 No 3 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: Coronavirus Disease 2019 (Covid-19) is currently a serious world problem with the number of cases always increasing every day. Methods: To find out the factors related to the incidence of coronavirus disease 2019 (Covid-19) in clinical practice, this research was conducted using a quantitative method equipped with a qualitative one and using a cross-sectional design. A sample of 198 people was selected using random sampling. The research instrument used a questionnaire. Out of 198 visitors at the clinic, Result: the respondents were female 53.0%, age > 18 years 70.2%, high school graduate education 86.4%, high minimum income 78.8%, knowledge good 50%, not densely occupied 86.4%, adequate ventilation 82.2% good room lighting 97.5%, washing hands 52.9%, maintaining cough etiquette 51.9%, wearing a mask 51.4%), not holding large-scale gatherings 80.8%, Not maintaining body immunity 69.2%, good facilities and infrastructure 80.8%, Access to remote services 53.0%, there is family support 59.6%. Conclusion: The chi-square test shows that there is a significant relationship between family support and the incidence of Covid-19, Washing Hands, Wearing a Mask, PSBB. Body Immunity, Cough Ethics, then the qualitative results show that almost all informants said they always prevent COVID-19 by implementing health protocols in the form of wearing masks and keeping a distance.

The Relation of TMPRSS2 Gene Polymorphism to COVID-19 Severity of Indonesian Population in Jakarta Erlin Listiyaningsih; Rizkyana Avissa; Shinta Dewi Permata Sari; Wening Tri Mawanti; Dewi Martalena
BIOEDUSCIENCE Vol 6 No 3 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: The COVID-19 disease caused by the SARS-CoV-2 virus has diverse symptoms, ranging from asymptomatic, mild symptoms such as flu-like illness and pneumonia to acute respiratory distress syndrome, which ends in death. Until now, the mechanism of the COVID-19 disease that causes widespread symptoms and the severity and factors that influence it are still unclear. During viral internalization, it needs to be cleaved by the serine protease encoded by the TMPRSS2 gene. It is hypothesized that higher expression of the TMPRSS2 gene causes higher virus internalization into cells, leading to more severe symptoms in patients. Methods: The Single Nucleotide Polymorphism Genotype Test was carried out to prove whether the TMPRSS2 gene affects the severity of COVID-19, as evidenced in other viral respiratory diseases. With a better understanding of gene expression related to this disease, it is hoped that we can better understand the mechanism of COVID-19 and establish better therapies and prevention against it. In this study, 68 COVID-19 patients participated and were categorized into two groups based on their clinical symptoms, namely mild symptoms without symptoms (n=12) and Moderate-Severe symptoms (n=56). PBMC cells were isolated from the patient. Then the DNA was extracted and used as a template in the SNP Genotyping of the TMPRSS2 rs2070788 gene variant. Results: The results showed that 35 samples had A/A homozygous genotypes, 29 A/G heterozygous samples, and 4 G/G homozygous samples. n=29) and heterozygous A/G (n=23), whereas only 4 were homozygous G/G. In addition, the homozygous G/G genotype was only detected in the moderate-severe group. Conclusions: A more significant number of samples from the asymptomatic mild symptom group is needed to statistically prove that homozygous G/G variants or G alleles are generally associated with the severity of COVID-19 patients.

Variasi Morfometrik Tetragonula laeviceps di Berbagai Habitat di Provinsi Sulawesi Tengah I Made Budiarsa; Fatmah Dhafir; Manap Trianto
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: Habitat is a place for living things to grow and develop. Differences in habitat in an area can affect the size of an organism. This study aims to determine the morphometric variations of Tetragonula laeviceps found in urban and forest habitats in Central Sulawesi Province. Methods: This research was conducted with roaming technique. Morphometric characters observed were 35 characters of stingless bee and analyzed using Microsoft Excel software. Results: The results of this study indicated that the morphometric size variation of T. laeviceps found in forest habitats (3.58 mm) is greater than that of wild bee in urban habitats (3.05 mm). Conclusions: Different habitat characteristics can affect the morphometry variations of the bee, especially in T. laeviceps species. The bee's morphometry body size is greater than in the forest habitat, because in that habitat there are environmental temperatures and types of plant sources of nectar which are more supportive for bee life compared to urban habitats.

Kajian Kondisi Keberadaan Anoa Dataran Rendah dengan Anoa Pegunungan di Pulau Sulawesi Ditinjau dari Jenis Pakannya Daffa Azalia; Intan Rachmawati; Ade Suryanda
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: Anoa is an endemic animal on Sulawesi island which is threatened with extinction, both lowland anoa (Bubalus depressicornis) and mountain anoa (Bubalus quarlesi) which share the status of Endangered. The aim of this study is to determine the condition of lowland anoa and mountain anoa on the Sulawesi island in terms of the type of feed. Methods: This research was accomplished using literature review techniques. Results: The outcome of this study showed a represent that lowland anoa in terms of feed type has an endangered status, as well as mountain anoa in terms of feed type, which is endangered. The status of the existence of lowland anoa and mountain anoa, both from the type of feed and the IUCN conservation organization, is equally threatened with extinction. The same condition and conservation status is obtained by the result because the two different anoas that lives in different habitats are able to adjust their own feed according to where the animals are. Conclusions: Although it has no bearing on anoa condition and conservation status, it is expected that the national park and captivity of anoa on Sulawesi Island can better sort food for lowland anoa and mountain anoa to support their physiological functions for their existence.

Optimasi Volume Kit Da An Gene Untuk Deteksi SARS-CoV-2 dengan Real Time RT-PCR Seprianto; Muhammad Arreza; Titta Novianti; Febriana Dwi Wahyuni; Oktaviani Naulita Turnip; Roaslein Putri; Henny Saraswati
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: SARS-CoV-2 is a new type of coronavirus of the genus Betacoronavirus and the family Coronaviridae that causes a respiratory disease called COVID-19. The virus has a sheath and genetic material in the form of single-chain RNA. The genome structure of this virus is divided into two types, namely genes that encode non-structural proteins consisting of the ORF1a / ORF1b gene and genes that encode structural proteins consisting of spike glycoprotein (S), envelope (E), membrane glycoprotein (M), and nucleocapsid protein (N). Methods: The method of detecting SARS-CoV-2 with real time RT-PCR is the most recommended method because it has high specificity and accuracy. The specificity of a method is necessary to be able to specifically recognize the pathogen that causes the disease. Real time RT-PCR requires sampling with a swab on the oropharynx or nasopharynx to be examined in the laboratory which later the presence of viral RNA becomes a molecule that is assessed for diagnosis results. In this study, volume optimization was carried out on the Da An Gene kit used for the detection of SARS-CoV-2 with Reverse Transcription Polymerase Chain Reaction (Real time RT-PCR) with the aim of saving the use of reagents from available kits but with amplification results remaining optimal and accurate. Results: There were three SARS-CoV-2 RNA samples used consisting of N62, N63, and N79 samples and three types of total volume used were 20 Î¼l, 15 Î¼l, and 10 Î¼l. The results of this study showed that the three positive samples contained SARS-CoV-2 with a Cq value of < 40. Conclusion: A volume of 20 Î¼l is the optimal volume, which is more efficient than the manufacturer's recommended volume of 25 ul.

Identifikasi Protein Plasmodium falciparum yang Tidak Berkarakter Melalui Analisis In-silico Vianney Widjaja; Albert Lim; Benedicta Aini; Gabrielle Audrey Gandasasmita; Jeremie Theddy Darmawan; Arli Aditya Parikesit
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: Numerous types of research on malaria were done over a long period of time but there are still some unknowns. However, it is globally known that malaria is caused by the Plasmodium parasite, mainly and most lethally by Plasmodium falciparum. The purpose of this research is to understand the structure and function of three uncharacterized P. falciparum proteins (PF3D7_1468000, PF3D7_1147400, PF3D7_1351100) using bioinformatic methods in hopes to learn more about malaria. Methods: The three uncharacterized P. falciparum proteins were inserted into Phyre2 for knowing the protein homology, InterPro, and SUPERFAMILY hidden Markov models for understanding the domain annotation, scanprosite for knowing the post-translational modification, Ramachandran plot for protein validation, and Yasara for visualizing the protein. Results: According to the Phyre2 results, the third protein showed the highest confidence and coverage level of 100%, followed by the second protein, and the lowest was the first protein. Interpro and SUPERFAMILY results identified the first protein as WD40 repeat superfamily, the second protein as Cytochrome C subunit II-like, and the third protein as CXXC motif. Scanprosite revealed all sequences possessing protein domains in which the first protein has three protein domains, the second protein has one protein domain, and the third protein has two protein domains. According to the Ramachandran plot, the first and second protein generally has an Î±-helix structure while the third protein has an overall Î²-sheet structure, which differs to some extent from the protein structure visualization. The three protein visualizations exhibited secondary structures and more than 50 amino acid residues for each protein. Conclusion: This research concluded that the second and third uncharacterized proteins (PF3D7_1147400, PF3D7_1351100) could be promising antimalarial drug targets leading to the P. falciparum parasite death.

Validation of Morphometric Teaching Materials with South Sumatera Local Fish Content Kodri Madang; Elvira Destiansari; Safira Permata Dewi
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: In the local context of South Sumatra, it is known that freshwater fish occupy important sociological and economic aspects. Placement of materials such as examples of belida fish, snakehead fish, betok fish and local species of fish from South Sumatra into the Morphometry course is something that will support the understanding of morphometry. The existence of this material will also meet the demands of the curriculum with contextual material. This study aims to explain the results of construct and material validation on the product of Morphometry subject teaching materials based on local Sumatran content. This research was conducted in the Biology Education Study Program. Methods: This type of research is descriptive qualitative using expert validation sheets. A validation sheet is given and an expert is assessed. Experts who provide assessments include material experts and constructivists. Validation includes aspects of the construct and material on the product of teaching materials.. Results: The validation results show that the results of the construct expert validation obtained 91.42% results and were declared valid/no need to be revised and the material expert validation results obtained 97.14% results and were declared valid/no need to be revised. Conclusions: Based on these results, it can be concluded that the product of teaching materials is declared valid by construct and material experts so that the product can be used for the next process. These results indicate that the teaching materials are in accordance with learning outcomes and can support the learning process of Morphometry Subjects.

Catatan Pertama dari Nectriopsis rexiana yang terbawa pada miselium Sphaerobolus stellatus Rudy Hermawan; Indra Maulana; Yuyun Nishaul Khairillah
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: Fungicolous fungi merupakan fungi mikro yang menghuni atau menempel pada fungi lain. Jamur ini dapat menembus daerah jaringan inang. Jamur fungicolous secara alami hidup pada inang di lapangan. Sphaerobolus stellatus basidioma ditemukan pada kayu busuk bersama dengan spesies lain seperti Ceratiomyxa arbuscula, Lentinus squarrosulus, Stemonitis pallida, dll. Banyak spesies jamur lain yang tidak muncul sebagai basidioma hidup di substrat atau bahkan di Sphaerobolus stellatus basidioma. Penelitian ini bertujuan untuk menganalisis fungicolous pada miselium Sphaerobolus stellatus menggunakan analisis molekuler. Metode: Miselium (sampel miselium SS) dipetik di lapangan dan dimasukkan ke dalam larutan CTAB. Kemudian, miselium diproses untuk identifikasi molekuler. DNA genom kemudian diamplifikasi menggunakan primer Internal Transcribed Spacer. Urutan dirakit dan dianalisis untuk uji homologi dalam NCBI BLAST. Kemudian dilanjutkan dengan rekonstruksi pohon filogenetik untuk ketiga jenis pohon filogenetik tersebut. Mereka adalah Neighbor Joining oleh MEGA, Maximum Likelihood oleh MEGA, dan Randomized Axelarated Maximum Likelihood. Hasil: Pohon filogenetik terbaik berasal dari pohon filogenetik Randomized Axelarated Maximum Likelihood. SSmycelium diidentifikasi sebagai Nectriopsis rexiana dengan nilai BS 75%. Spesies tidak memiliki deskripsi morfologi, hanya sumber DNA, dan urutan ITS. Kesimpulan: Nectiropsis rexiana dikenal sebagai jamur parasit dan fungicolous pada jamur lain. Penelitian ini merupakan catatan pertama bahwa Nectriopsis rexiana ditemukan di Sphaerobolus stellatus.

Condition Factors and Growth Patterns of Mantis Shrimp (Harpiosquilla raphidea) in the Estuary Waters of Berombang River, Labuhanbatu Regency, North Sumatera Yulistiani Yulistiani; Rivo Hasper Dimenta
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: Mantis shrimp is one of the favorite shrimp caught by fishermen around the estuary waters of Berombang River, Labuhanbatu Regency. This study aimed to determine the condition factors and growth patterns of mantis shrimp population. Method: Three station points were determined using a purposive random sampling method. The sampling of Harpiosquilla raphidea was carried out using trawl nets. The data analysis provided information on growth patterns and condition factors. Result: The results showed that the growth patterns of male H. raphidea belonged to the positive b> 3 allometric category, where weight growth was faster than carapace growth. Moreover, the growth patterns of female H. raphidea belonged to the negative b<3 allometric category, where the carapace growth was faster than body weight growth. The value of Fulton (K) condition factors for male H. raphidea ranged from 3.54 to 13.18, and for females, it ranged from 15.28 to 22.09. The value of correlation analysis of Physic-chemical parameters, water temperature (0.935) and DO (0.832) showed the high category correlation test results. Conclusion: This result showed that from November 2021 to January 2022, the condition of H. raphidea population around the estuary waters of Berombang River was known and predicted to be in the adult stage

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Contact Name
Susilo

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aru_palaca@yahoo.com

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rantimouri@gmail.com

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Kota adm. jakarta timur,

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