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INDONESIA
BIOEDUSCIENCE
Published by Universitas Muhammadiyah Prof. Dr. Hamka
ISSN : 2614154X     EISSN : 26141558     DOI : -
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education
BIOEDUSCIENCE is an open access journal that publishes research in the field of Biology and Biosain Education such as: Applied and implemented in education and learning, Botany, Zoology, Microbiology, Ecology, Biotechnology, Molecular Biology, Genetics, Bioinformatics, Cell and Developmental Biology, Biodiversity and Bioconservation. BIOEDUSCIENCE is published by Biology Education Study Program, Universitas Muhammadiyah Prof. Dr. Hamka, Indonesia regularly in June and December. ISSN : 2614-1558; P-ISSN : 2614-154X.
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Articles 544 Documents
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Literasi Lingkungan Masyarakat Adat dan Masyarakat Perkotaan di Pulau Lombok Mouria Bidarinjani; Abdulkadir Rahardjanto; Dwi Setyawan
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11048

Abstract

Background: The high ability of community environmental literacy can form ideal environmental conditions, but indigenous peoples and urban communities have different understandings of environmental conditions. This study aimed to determine differences in the environmental literacy of indigenous peoples and urban communities on Lombok Island. Methods: The objects of this research are the indigenous people of Bayan Village, North Lombok, and the people of the Dasan Sari Environment, Mataram City, West Lombok: quantitative research methods, quantitative descriptive approaches, and survey research types. Data collection tools are observation, interviews, and questionnaires. Data analysis using SPSS, data analysis techniques by calculating the mean score of the questionnaire. Results: The study obtained a mean environmental literacy value for the indigenous people of Bayan Village at 214.37 and for the Dasan Sari Environmental community at 240.56. The results of the hypothesis testing in this study obtained a significance value greater than 0.05 and t-count < t-table. Conclusions: There was no real difference in environmental literacy between indigenous peoples and urban communities on Lombok Island.
Growth of Red Spinach (Amaranthus tricolor L.) Hydroponically Using POC Tomatoes and Moringa Leaves Sepbrie Mulia Bingah Pranata; Suparti Suparti
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11688

Abstract

Background: Red spinach (Amaranthus tricolor L), one of the plants contains several important elements with high nutritional value, is liked by the community, easy to cultivate with hydroponics. Efforts to determine the growth of red spinach by administering POC as a nutrient needed by plants. The purpose of this study was to find out how red spinach plants grow in POC of a mixture of tomato extract and moringa leaf extract with the addition of coconut water hydroponically and at what concentration the most effective POC. This research was conducted at the Biology Green House FKIP UMS. Methods: this study used an experimental method with a completely randomized design (CRD) with 2 factors, namely factor 1 type of POC extract mixture and factor 2 the difference in the volume of POC so that from both factors 6 treatments were obtained with three replications for each treatment. Results: Data analysis using Two Way Anova showed that the results of the C2V2 treatment had a plant height of 2.13 cm, root length of 1.96 cm, and the highest number of leaves with 4 strands. The C1V3 treatment produced the lowest plant height, namely 1.07 cm and the C2V3 treatment produced the lowest root length, namely 0.73 cm and the C1V1, C1V3, C2V1, C2V3 treatments showed the lowest number of leaves, only 2 leaves. Conclusion: The POC of tomatoes and Moringa leaves which were given the same treatment showed that the POC of Moringa leaves were effectively used as red spinach growth nutrients.
The Effect of Banana Stem Powder on the Productivity of the Brown Oyster Mushroom (Pleurotus cystidiosus) Rizky Nurhayati; Suparti Suparti
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11699

Abstract

Background: Brown Oyster Mushroom (Pleurotus cystidiosus) is a type of mushroom that has different advantages from other oyster mushrooms. Generally, farmers make planting media for brown oyster mushrooms made from sawdust of sengon wood. The availability of raw materials for sawdust of sengon wood is increasingly difficult to obtain. So we need other alternative materials as a source of nutrition, one of which is banana stem waste. This study aims to determine the effect of banana stem flour on the diameter of the cap, the number of fruiting bodies, and the wet weight of the fungus. Methods: This study used a completely randomized design (CRD) which consisted of one factor. This study used 4 treatments and 3 replications. Data was tested by quantitative descriptive analysis using the Analysis of Variance (ANOVA) test. Results: Banana stem flour has a significant effect on all parameters. The JM2 treatment resulted in a cap diameter of 10.3 cm, mushroom fruiting bodies of 10.6 and wet mushroom weight of 103.8 g. Conclusion: The addition of banana stem powder can increase productivity in the parameters of mushroom cap diameter, number of mushroom fruiting bodies, and wet weight of mushrooms, which can be used by readers or researchers. Utilization of waste such as sawdust of sengon wood and waste of banana fronds for the cultivation of brown oyster mushrooms is one way of handling plantation waste.
Bacteria Isolation from Public Cemeteries Soil and Test for Resistance to Antibiotics Anggita Juniar Laspartriana; Triastuti Rahayu; Erma Musbita Tyastuti; Yasir Sidiq
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11740

Abstract

Background: A public burial place (TPU) is used to bury the dead. There is an active decomposition of corpses in the soil, which produces soil nutrients and minerals that can support the growth of microorganisms in the ground, including pathogenic bacteria. Pathogenic bacteria have a more severe impact if they are resistant to antibiotics. Methods: Soil samples were taken in the Bonoloyo TPU area, Surakarta, Central Java, at 3 points each of 2 depths of 20 and 50 cm. Soil samples were inoculated on NA (Nutrient agar) media using the spread plate method. After 48 hours, colony counting and morphology observations were carried out, followed by gram staining. Isolated bacterial isolates were tested for resistance to 3 types of antibiotics. Results: The average population of soil bacteria in blocks 12, 17, and 21 at a depth of 20 cm is 4 x 10⁶ CFU/g; 8 x 10⁶ CFU/g; and 1 x 10⁶ CFU/g, while at a depth of 50 cm, it is 2.3 x 10⁶ CFU/g; 6 x 10⁶ CFU/g; and 4 x 10⁶ CFU/g. The morphology of bacterial colonies is irregular (26 isolates), flat elevation (19 isolates), and the color of isolates is predominantly white. The Gram staining results obtained 23 isolates are Gram-negative, and 22 are Gram-positive with a dominant cell form in cocci. Antibiotic resistance tests showed that the bacterial isolates were resistant to ampicillin (28.9%), Bacitracin (64.4%), and Cefepime (57.8%). Conclusions: Burial soil bacterial populations at Bonoloyo TPU at different depths did not differ markedly, with numbers ranging from 1 - 8 x 10⁶ CFU/g.
Screening and Identification of Cellulolytic Bacteria from Public Cemetery Soil Siti Nur Syarifah; Triastuti Rahayu; Erma Musbita Tyastuti; Yasir Sidiq
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11745

Abstract

Background: A public cemetery (TPU) is where bodies are buried. Before being buried, the body is wrapped in a shroud or full clothes and sometimes put in a coffin. These materials contain cellulose, a substrate for cellulolytic microorganisms, including bacteria, to decompose. Exploration of cellulolytic bacteria in TPU has not been found, even though TPU holds potential cellulolytic bodies. This study aims to determine the cellulolytic potential and identify bacterial isolates from TPU. Methods: 36 bacterial isolates tested for cellulolytic potential have been isolated from TPU Pracimaloyo Kartasura, Sukoharjo. Selection of cellulolytic bacteria used selective Carboxymethyl Cellulose (CMC) media dripped with Congo red 0.1%, while identification was based on colony morphology and Gram staining. Results: This study obtained one isolate (2.78%) showing high cellulolytic activity with a cellulolytic index (IS) 7, namely P14, 24 isolates (66.67%) in the "moderate" category, four isolates (11.11%) with category "low," and seven isolates (19.44%) did not show cellulolytic ability. Isolates cellulolytic positive have a shiny white colony color, entire edges, raised elevation, and belong to the Gram-negative coccus form. Conclusions: From the results of this study, it can be concluded that public burial sites (TPU) harbor potential cellulolytic bacteria.
Test of Hypersensitivity and Antagonistic Reaction of Endophytic Bacteria from Klutuk Banana (Musa balbisiana) Ratih Dewianty; Triastuti Rahayu; Yasir Sidiq
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11795

Abstract

Background: Plants’ response and antagonistic reaction against disease-causing organisms are two crucial characteristics of Plant Growth Promoting Bacteria (PGPB). Seventeen endophytic bacteria have been isolated from Musa balbisiana. However, the plants’ reaction against these isolates and their antagonistic activities against disease-causing organisms remained unknown. This study aims to (1) determine the sensitivity effect of endophytic bacteria isolates to tobacco and (2) test the antagonism of endophytic bacteria isolates against the blast disease actor, namely Pyricularia oryzae. Method: Sensitivity tests were performed by inoculating bacterial isolates into tobacco leaves with infiltration. Bacterial isolates were prepared with a minimum OD 600: 0.5. Then, a milliliter volume of each isolate was infiltrated into tobacco leaves from the abaxial side using a 3 mL syringe. The lesion on the tobacco leaves was observed seven days after inoculation (DAI). The antagonism test was carried out by growing Pyricularia oryzae on a PDA plate for seven days, subsequently transferred to an NA medium for three days then inoculated with endophytic bacteria. The assessed parameter of the antagonistic test was the formation of an inhibitory zone between endophytic bacteria and Pyricularia oryzae at 4 DAI. Results: No hypersensitive reaction of tobacco leaves against K117, K324, K38, K86, K18, K28, K102 isolates inoculation at 7 DAI. Furthermore, the antagonistic test indicated that all isolates inhibited the growth of Pyricularia oryzae, with the range of inhibition from 32.36 to 40.46%. Implication: Thus, these results revealed the PGPB characteristics in the newly isolated endophytic bacteria from the banana.
The Effect of Lamtoro Leaf POC and Tofu Liquid Waste on the Growth of Cayenne Chili (Capsicum frutescens) Siska Andriyani; Suparti Suparti
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11885

Abstract

Background: Liquid organic fertilizer is a fermented solution of organic plant residues, animal and human feces containing nutrients. Lamtoro and tofu liquid waste contain N, P, K, Mg, and Ca, which are suspected to have the potential for liquid organic fertilizer to increase the growth of cayenne pepper (C. frutescens). This study aims to determine the effect of giving POC from lamtoro leaves and tofu liquid waste on the growth of cayenne pepper (C. frutescens). Methods: This study used a completely randomized design (CRD) with two replications. POC volume 1:1 consisted of two-liter lamtoro leaf extract and 2-liter tofu liquid waste. The first factor is the volume concentration of POC with the respective concentrations of V0: 0%, V1: 10%, V2: 20%, and V3: 30%. The second factor is the time of spraying POC T1: 3 days interval and T2: 6 days interval. Parameters measured included plant height (cm), number of leaves (strands), and plant fresh weight (g). Results: 20% volume (V2) with three-day intervals (T1) gave the best results for plant height (20.10 cm), number of leaves (7.00 leaves), and plant fresh weight (11.50 g). 0% volume (V0) with an interval of 6 days (T2) gave the lowest yield on plant height (6.35 cm), number of leaves (3.00 leaves), and fresh weight of plants (3.00 leaves). Conclusions: Application POC from lamtoro leaves and tofu liquid waste can potentially increase the growth of cayenne pepper (C. frutescens).
The Influence of Inquiry Lesson Learning on Students' Scientific Reasoning Ability in Ecosystem Material Melani Ajijah; Aa Juhanda; Setiono Setiono
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11964

Abstract

Background: This study aims to determine the influence of applying the Inquiry Lesson learning model on the scientific reasoning abilities of Grade X students. Method: This type of research uses a quasi-experiment. The sample for this research was A total of 55 students in the science program grade 10 at one of the Madrasah Aliyah Public Schools (MAN) were involved. The instrument used is in the form of scientific reasoning ability test questions in the form of reasoned multiple-choice questions of 15 questions. The research design used is the Non-equivalent Control Group. The second instrument uses a student response questionnaire to learning using the inquiry lesson learning model, which consists of 12 questions. Results: The average N-Gain score was 0.48 in the moderate category, and the scientific reasoning abilities of students in the control class produced an average pretest score of 56.12 and a post-test average score of 70.13 with an average score of 70.13. N-Gain score of 0.32. The hypothesis test obtained a significance value of sig (2-tailed) 0.004, then H0 was rejected, and H1 was accepted. The emergence of scientific reasoning abilities of experimental class students on each indicator varies but generally has an N-Gain score in the moderate category. Student response questionnaires regarding the inquiry lesson learning model showed an average score in the excellent class (61-80). Conclusion: the conclusion in this study is that there is an increase in inquiry lesson learning on students' scientific reasoning abilities in Grade X ecosystem material.
Pharmaceutical Activity of Medicinal Plant Amaranthus viridis Linn. Due to Its Chemical Constituents: A Review Muhammad Ikram; Ali Haider; Urooj Fatima
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12089

Abstract

Background: Amaranthus viridis, a herbal plant belonging to the family Amaranthaceae, has gained significant attention in the field of traditional medicine due to its rich chemical composition. This plant is known to contain various bioactive components that contribute to its pharmaceutical value. With its extensive range of chemical compounds, Amaranthus viridis has found applications in therapeutic, pharmaceutics, and cosmetic industries. In this study, we aim to explore the medicinal potential of Amaranthus viridis and investigate its various pharmaceutical activities. Methods: The process of writing this review involved conducting comprehensive electronic research using reputable sources, including international and national article databases, along with books accessed through platforms like Google Scholar, PubMed, IEEE Xplore, ScienceDirect and others. The gathered data is subsequently analyzed, followed by employing synthetic approaches with narrative methods to derive meaningful conclusions and interpretations. Results: Amaranthus viridis displays potent antioxidant and anti-inflammatory properties, attributed to its rich composition of flavonoids, cardiac glycosides, and zinc. Its diverse pharmaceutical activities make it a promising contender for medicinal use, addressing labor pain, skin conditions like eczema and psoriasis, and respiratory issues, while also exhibiting antimicrobial effects against bacteria, fungi, and parasites. It can be accomplished by Extraction with solvent, Phytochemical screening and Isolation and purification. Conclusion: Amaranthus viridis, a herbal plant, shows significant potential in therapeutic, pharmaceutics, and makeup industries. Its bioactive compounds, including flavonoids, cardiac glycosides, and zinc, contribute to its diverse pharmaceutical activities, such as antioxidant, anti-inflammatory, antibacterial, antifungal, antidiabetic, and antihelmintic properties.
Novel CRISPR-Cas9 approach to combat with COVID-19 Jahanzaib Ali
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12275

Abstract

Background: The recent outbreak of Corona Virus has affected people all over the world so that WHO declared it a pandemic. This pandemic has become a major problem for people, health professionals, and research institutes that how to combat SARS-CoV-2. The objective of this review article is to provide a brief study that CRISPR/Cas9 can be used to combat SARS-CoV-2. Methods: The comparison of three genome editing techniques (ZNF, TALEN, and CRISPR/Cas9) is done. The mechanism of action of CRISPR/Cas9 is discussed. Results: The studies provided in this review article suggest that it is difficult to recognize this virus as they have their own metabolic machinery and they replicate their selves in the host cell and consume host cellular products to perform their own functions. The COVID-19 proteins may contain some specific areas that are suitable targets for therapy, such as tiny inhibitor molecules for viral polymerase, or impede the attachment of viruses to the receptor sites, for example, viral coat proteins can be used for vaccination purposes. Conclusion: CRISPR-Cas9 can be used to control the SARS-CoV-2 genome from replication and spread to other parts of the body as it can edit the genome quite efficiently. This proposed model will help in targeting the SARS-CoV-2 genome more precisely in the future.

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