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Jurnal Biologi Tropis
Published by Universitas Mataram
ISSN : 14119587     EISSN : 25497863     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Jurnal Biologi Tropis (ISSN Cetak 1411-9587 dan ISSN Online 2549-7863) diterbitkan mulai tahun 2000 dengan frekuensi 2 kali setahun oleh Program Studi Pendidikan Biologi PMIPA FKIP Universitas Mataram, berisi hasil penelitian dan ulasan Ilmiah dalam bidang Biologi Sains.
Arjuna Subject : -
Articles 2,562 Documents
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Keanekaragaman Spesies dan Zonasi Habitat Echinodermata di Perairan Pantai Semerang, Lombok Timur (Diversity of Species and Habitat Zonation of Echinoderm in Semerang Coastal Waters, East Lombok) Noar Muda Satyawan; Yusli Wardiatno; Rahmat Kurnia
Jurnal Biologi Tropis Jurnal Biologi Tropis. Vol.14 No. 2 Desember 2014
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (421.132 KB) | DOI: 10.29303/jbt.v13i1.66

Abstract

Research was conducted in order to determine the diversity of species and habitat zonation of Echinodermata in Semerang coastal waters East Lombok. The field survey was conducted using transects kuadrad and free collection during March to June 2013. Based on the results, in the Semerang Coast was found 26 species of Echinoderm which consist of Echinoidea (13 species), Asteroidea (7 species), Ophiuroidea (3 species ) and Holothuroidea (3 species). Echinoderms habitat in Semerang divided into 5 zones, consist of reef flat, sand, seagrass beds, rubble, and coral reefs. Diversity index values differ in each habitat. Shannon - Wiener index (Hꞌ) were largest on coral reef habitats (H ꞌ = 2.59 )and then followed by sand (H ꞌ = 2.30), rubble (H ꞌ = 2.17), seagrass (H ꞌ = 1, 93) and the reef flat (H ꞌ = 1.48). Greatest Evenness index was found in the sand habitat (E = 0.96) followed by a reef flat (E = 0.92), coral reefs (E = 0.86), rubble (E = 0.75) and seagrass beds (E = 0.67). the largest found Margalef Index (R), was found in coral reef habitats (R = 5.02) and then followed by rubble (R = 4.07), sand (R = 3.79), seagrass bed (R = 3.62) and reef flat (R = 1.41). Class of Echinoidea has found in all zones, Asteroidea and Holothuroidea not found on the reef flat zone while Ophiuroidea only found in the rubble and the coral reef zone.
KARAKTERISTIK HABITAT UNDUR-UNDUR LAUT (FAMILI HIPPIDAE) DI PANTAI BERPASIR, KABUPATEN CILACAP Yusli Wardiatno; I Wayan Nurjana; Ali Mashar
Jurnal Biologi Tropis Jurnal Biologi Tropis. Vol.14 No. 1 Juni 2014
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v14i1.1947

Abstract

terlampir
Mode of Action Anti Serangga dari Tananam Jayanti (Sesbania sesban L. Merr.)(MAGNOLIOPSIDA: FABACEAE) Suripto Suripto; Tresnani, G.; Gunawan, E. R.
Jurnal Biologi Tropis Vol. 20 No. 2 (2020): Mei - Agustus
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.34 KB) | DOI: 10.29303/jbt.v20i2.1875

Abstract

Abstrak: Dari hasil penelitian-penelitian sebelumnya telah diketahui, bahwa tanaman Jayanti (Sesbania. Sesban) memiliki aktivitas anti serangga terhadap Plutella xylostella, salah satu jenis serangga hama penting tanaman kubis. Juga telah diketahui, bahwa bahan aktif anti serangga dari S. sesban dengan konsentrasi tertentu dapat mematikan imago Diadegma semiclausum, yang merupakan musuh alami dari P. xylostella. Namun demikian, mode-mode aksi aplikasi bahan insektisida dari S. sesban yang selektif untuk pengendalian serangga hama, yaitu dapat menekan populasi P. xylostella namun aman terhadap populasi D. semiclausum belum diketahui. Penelitian ini bertujuan untuk mengetahui mode-mode aksi aplikasi berbagai fraaksi ekstrak daun S. sesban yang selektif untuk pengendalian P. xylostella.  Serbuk kering daun S. sesban diekstraksi secara bertingkat menggunakan seri pelarut, yaitu berturut-turut petroleum eter, diklorometana, etanol dan air. Masing-masing fraksi ekstrak daun S. sesban dilakukan bioassay terhadap  P. Xylostella secara parallel,  yaitu uji mortalitas larva, uji daya tolak ovipositor, uji penghambatan penetasan telur dan uji anti feedant. Data hasil setiap uji hayati diolah dengan analisis probit untuk menentukan LC50 (mortalitas larva) dan EC50 (daya tolak ovipositor, penghambatan tetas telur dan daya anti feedant). Hasil menunjukkan,  bahwa fraksi ekstrak-etanol daun S. sesban memiliki daya anti serangga terhadap P. xylostella lebih tinggi daripada fraksi-fraksi ekstrak lainnya. Hasil juga menunjukkan, bahwa aplikasi fraksi ekstrak-etanol daun S. sesban melalui mode-mode  aksi  anti ovipositor dan anti feedant adalah selektif untuk pengendalian  P. xylostella. Harga EC50 daya tolak ovipositor (20.52 ppm) dan EC50 anti feedant (26.77 ppm) terhadap P. xylostella masing-masing lebih kecil daripada harga LC50 (37.38 ppm) terhadap D. semiclausum. Disarankan, bahwa penggunaan ekstrak daun S. sesban untuk pengendalian P. xylostella hendaknya diarahkan untuk aplikasi menolak oviposisi dan menghambat aktivitas makan dan tidak disarakan untuk aplikasi mematikan larva dan menghambat penetasan telur P. xylostella. Juga disarankan, bahwa selektivitas ekologis dan efektivitas pengendalian P. xylostella dengan insektisida dari S. sesban perlu dievaluasi dengan mempelajari lebih lanjut stabilitas anti serangga dari S. sesban selama penyimpanan serbuk kering daun sebelum diekstraksi, penyimpanan ekstrak sebelum diaplikasikan, dan stabilitasnya selama aplikasi.Kata kunci: Diadgma semiclausum, mode aksi anti serangga, Plutella xylostella, Sesbania sesban.Abstract: From the results of previous studies have been known that Jayanti (Sesbania sesban) plants have anti-insect activity against Plutella xylostella, one of the important species of insect pests of cabbage. It is also well known, that the anti-insect active ingredient from S. sesban with a certain concentration can kill Diadegma semiclausum imago, which is a natural enemy of P. xylostella. However, selective modes of action for the application of insecticides from S. sesban for pest control, which suppress P. xylostella populations but are safe against D. semiclausum are not yet known. The S. sesban leaf dry powder was extracted stratified using a series of solvents, namely petroleum ether, dichloromethane, ethanol and water, respectively. Each fraction of S. sesban leaf extract was bioassayed on P. xylostella in parallel, namely larval mortality, ovipositor resistance, egg hatching inhibition and anti-feedant tests. Data on the results of each bioassay was analyzed by probit analysis to determine LC50 (larval mortality) and EC50 (ovipositor resistance, egg hatching inhibition and anti-feedant power. The results show that the extract fraction-ethanol of S. sesban leaves has higher insect repellent ability against P. xylostella than other extract fractions. The results also showed that the application of S. sesban leaf extract fraction-ethanol through each mode of action of anti-ovipositor and anti-feedant was selective for the control of P. xylostella. Each of the reject ovipositor EC50 (20.52 ppm) and anti-feedant EC50 (26.77 ppm) values against P. xylostella was smaller than the LC50 price (37.38 ppm) against D. semiclausum imago. It is recommended that the use of S. sesban leaf extract for controlling P. xylostella should be directed with application to reject oviposition and inhibit eating activities. This is because the concentration of the extract application is safe against D. semiclausum. Application of S. sesban leaf extract is not recommended to kill larvae and inhibit hatching eggs of P. xylostella because it requires a greater concentration of extract application and can suppress the population of D. semiclausum. Ecological anti-insect selectivity and effectiveness of using insecticide from S. sesban for the control of P. xylostella need to be evaluated by further studying the stability of the anti-insect active ingredient from S. sesban during storage of dried leaf powder before extracting, storage of extracts before being applied, and their stability during application.Keywords: Diadegma semiclausum, insecticidal mode of action, Plutella xylostella, Sesbania sesban.
Minyak Atsiri Daun Kari (Murraya koenigii (L.) Spreng) Sebagai Penghambat Pertumbuhan Bakteri Streptococcus pyogenes dan Shigella dysenteriae Agustin Rosa Fadila; Yeni Mariani; Fathul Yusro
Jurnal Biologi Tropis Vol. 20 No. 2 (2020): Mei - Agustus
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.384 KB) | DOI: 10.29303/jbt.v20i2.1756

Abstract

Abstrak: Tanaman kari (Murraya koenigii (L.) Spreng) merupakan tanaman budidaya di Kalimantan Barat dan memiliki potensi besar sebagai tanaman penghasil minyak atsiri yang memiliki sifat antibakteri. Tujuan dari penelitian ini adalah menganalisis rendemen minyak atsiri daun kari dan potensinya dalam penghambatan pertumbuhan bakteri S. pyogenes dan S. dysenteriae. Penelitian menggunakan metode penyulingan daun kari dengan cara destilasi uap, perhitungan rendemen dan pengujian aktivitas antibakteri S. pyogenes dan S. dysenteriae pada media MHA dengan metode difusi cakram. DMSO digunakan sebagai kontrol negatif, amikacin sebagai kontrol positif dan minyak atsiri daun kari pada empat level konsentrasi yaitu 5, 10, 15 dan 20%. Analisis data menggunakan analisis one-way anova pada daya hambat. Hasil penelitian menunjukkan bahwa rendemen minyak atsiri daun kari yang dihasilkan dalam penelitian ini sebesar 0,139%. Tingkat penghambatan terhadap bakteri S. pyogenes tergolong kuat pada semua level konsentrasi dan pada bakteri S. dysenteriae tergolong sedang pada konsentrasi 10, 15 dan 20%. Hasil penelitian membuktikan bahwa minyak atsiri daun kari berpotensi sebagai antibakteri alami.Kata kunci: Minyak atsiri, tanaman kari (Murraya koenigii (L.) Spreng), rendemen, antibakteri, Streptococcus pyogenes, Shigella dysenteriaeAbstract: Curry plants (Murraya koenigii (L.) Spreng) plants recently cultivated in West Kalimantan have enormous potential as a plant producing essential oils and has antibacterial properties. This study aims to analyze the yield of curry leaf essential oil and its potential in inhibiting the growth of S. pyogenes and S. dysenteriae bacteria. The research method used is the distillation of curry leaves by steam distillation, calculation of yield, and testing of the antibacterial activity of S. pyogenes and S. dysenteriae on MHA media by disk diffusion method. DMSO was used as a negative control, amikacin as a positive control and essential oils of curry leaves at four concentration levels (5, 10, 15, and 20%). The inhibition zone data were analyzed using one-way ANOVA. The yield of curry leaf essential oil produced in this study was 0.139%. The level of inhibition against S. pyogenes bacteria was classified as strong at all concentration levels, and in S. dysenteriae was classified as moderate at concentrations of 10, 15, and 20%. The research proves that curry leaf essential oil has proven potential as natural antibacterial.Keywords: Essential oils, curry plants (Murraya koenigii (L.) Spreng), yield, antibacterial, Streptococcus pyogenes, Shigella dysenteriae
Selection of Peanut Skin as a Growing Medium for Moon Orchid (Phalaenopsis amabilis) Nur Rokhimah Hanik; Sri Harsono; Anwari Adi Nugroho
Jurnal Biologi Tropis Vol. 20 No. 2 (2020): Mei - Agustus
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (744.919 KB) | DOI: 10.29303/jbt.v20i2.1896

Abstract

Ferns are a planting medium that is commonly used in Phalaenopsis cultivation, but the growing demand for fern will increase the selling price and the availability of fern is limited. So a solution is needed to reduce the maintenance and maintenance costs by utilizing peanut shells as a growth medium. The purpose of this study is: 1. To determine the effect of peanut skin media on the growth of Phalaenopsis amabilis month orchid 2. To find out better which influence between fern media, fern media plus peanut skins, or peanut skin media on orchid growth month of Phalaenopsis amabilis. When the research began in May 2019 until October 2019. The research object was in the form of a 4-5 month-old orchid, the research location in the Biology Laboratory of the Faculty of Veterinary Science, Bangun Nusantara University, Sukoharjo. Simple randomized study design with three treatments. Treatment A by giving fern media, treatment B by giving fern media mixed with peanut skin, and treatment C by giving peanut skin media. The research variables are the number of leaves, leaf length, leaf area, number of roots and root length measured once a week for 8 weeks. Data analysis with 2. Variance Test (Anova or Kruskal Wallis test) 3. Duncan Multiple Range Test (DMRT) or Moods Median Test. From the results of the study it was found that all observed growth variables (number of leaves, leaf width, leaf length, leaf area, number of roots, average root length, and wet weight) were treated with fern, mixed (Fern and peanut shell) media, and media of peanut skin, there was no significant difference in the effect on the growth of the month orchid. Which means that the three types of media both have an influence on the growth of Phalaenopsis amabilis month orchids. media ferns and peanut shells have their advantages and disadvantages of each. So it can be concluded that, 1. The use of peanut skin media can affect the growth of Phalaenopsis amabilis month orchids. 2. Media fern, a mixture of fern media and peanut skins, and peanut skin media can provide the same effect on the growth of Phalaenopsis amabilis month orchids. From the results of the study it can be recommended that peanut skins can be used as a medium or a mixture of growing media of Phalaenopsis amabilis orchids.
Potensi dan Pemanfaatan Satwa liar di kampung Pasir Putih kabupaten Fakfak Papua Barat Deny Anjelus Iyai; Yusak Sada; Johan F. Koibur; Aisyah Bauw; Meliza Worabay; Muhammad J. Wajo; Stepanus Pakage; Hengky Wambrauw
Jurnal Biologi Tropis Vol. 20 No. 2 (2020): Mei - Agustus
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.382 KB) | DOI: 10.29303/jbt.v20i2.1788

Abstract

Abstrak: Indonesia merupakan negara megabiodiversiti terutama jenis mamalia, reptilian dan avifauna. Penelitian ini bertujuan untuk mengetahui intensitas pemanfaatan satwa liar di kampung Pasir Putih, Distrik Fakfak Tengah, Kabupaten Fakfak.  Dalam penelitian ini objek pengamatan adalah potensi dan pemanfaatan satwa liar di kampung Pasir Putih Kabupaten Fakfak. Variabel pengamatan yang digunakan meliputi informasi dasar tentang jenis satwa liar, status satwa liar berdasarkan IUCN, tujuan pemanfaatan, cara berburu, frekuensi berburu, tingkat keberhasilan berburu, dan jumlah anggota berburu. Data yang diperoleh dianalisis secara tabulasi dan deskriptif untuk memperoleh gambaran objektif kondisi satwa liar yang ada di kampung Pasir putih Kabupaten Fakfak, provinsi Papua Barat. Hasil penelitian diketahui bahwa penduduk di kampung Pasir putih, masih melakukan aktivitas berburu walaupun tidak intens. Beberapa hewan yang dimanfaatkan meliputi hewan avifauna, mamalia dan reptil (herpet). Beberapa hewan masuk dalam kategori terancam punah (VU) oleh Redlist IUCN. Perburuan relatif sulit menggunakan jerat dan anjing berburu, frekuensinya relatif masih rendah dan dengan itu tidak dilakukan secara kolegial karena tingkat kesulitan.Kata kunci: perburuan; satwa liar; pemanfaatan satwa liar; redlist IUCN; Fakfak.Abstract: Indonesia is a mega biodiversity country especially mammals, reptils and avifauna. This study aims to determine the intensity of wildlife utilization in the village of Pasir Putih, Central Fakfak District, Fakfak Regency. In this study the object of observation is the potential and utilization of wildlife in the Pasir Putih village of Fakfak Regency. Observation variables used included basic information about wildlife species, wildlife status based on IUCN, purpose of use, hunting methods, hunting frequency, hunting success rate, and number of hunting members. The data obtained were tabulated and descriptive analyzed to obtain an objective picture of the condition of wild animals in the Pasir Putih village of Fakfak Regency, West Papua province. The results of the study revealed that residents in the white sand village, still doing hunting activities although not intense. Some of the animals used include Avifauna, mammals and reptiles (herpet). Some animals are in the endangered category (VU) by the IUCN Redlist. Hunting is relatively difficult using snares and hunting dogs, its frequency is still relatively low and hence it is not done collegially because of the difficulty level.  Keywords: hunting; wild animal; utilization of wild animals; IUCN redlist; Fakfak.
Biodiversity of Medicinal Plants in Tawang Serimbak Forest, Ensaid Panjang Village, Sintang Regency Fathul Yusro; Gusti Hardiansyah; Erianto Erianto; Yeni Mariani; Aripin Aripin; Hendarto Hendarto; Denni Nurdwiansyah
Jurnal Biologi Tropis Vol. 20 No. 2 (2020): Mei - Agustus
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (519.866 KB) | DOI: 10.29303/jbt.v20i2.1933

Abstract

Non-forest estate (APL) such as Tawang Serimbak need to be maintained because they store various flora that is useful for human life, one of which is medicinal plants. The research objective is to identify, analyze the potential, and to know the protection status of medicinal plants found in the Tawang Serimbak forest. The method of research were making a 100x100 m (square) cluster. At the center of the square placed a circular plot within 0.1 Ha (0.5 Ha of each cluster). Four circular subplots were made in each plot consisting of 1 m radius seedling subplot, 2 m for stake subplot, pole subplot (5 m radius), and 17.8 m for tree subplot. Data of species, efficacy, and protection status (IUCN) were identified, and the potency of plants were analyzed using plants density (number of plants/Ha). Tawang Serimbak forest has been identified as having 49 species of medicinal plants. Some species have great potentials such as Galearia fulva, Shorea uliginosa, and Alseodaphne sp. A total of 9 species of medicinal plants were identified in protected status, two of which were endangered; namely, Lithocarpus maingayi, Shorea uliginosa, and one other species with Endangered (critical) status, namely Santiria rubiginosa. Protection of forests in APL by the community can maintain the biodiversity of medicinal plants. The existence of the Tawang Serimbak forest needs to be protected and turned into a forest with high conservation value (HCV).
Keberadaan Tempat Pembuangan Akhir Berdampak pada Kualitas Air Abdonia W. Finmeta; Nur Aini Bunyani; Joritha Naisanu
Jurnal Biologi Tropis Vol. 20 No. 2 (2020): Mei - Agustus
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v20i2.1389

Abstract

Abstrak: Tumpukan sampah dan lindi secara langsung dan tidak langsung dapat menganggu kesehatan lingkungan dan keseimbangan ekosistem perairan. Penelitian ini bertujuan untuk mengkaji  kualitas fisik, kimia dan biologi air sumur pada berbagai jarak dari Tempat Pembuangan Akhir (TPA) kecamatanAlak serta dampaknya bagi kesehatan penduduk.Penelitian  menggunakan metode stratified random sampling yaitu penentuan berdasarkan jarak 400m-1200m dari timbunan sampah dari lokasi pengambilan sampel air sumur. Hasil analisa  sampel  menentukan kualitas air dengan membandingkanbaku mutu Air. Analisis pengaruh jarak TPA terhadap kualitas air selanjutnya dihitung nilai determinasi (r2) untukmenentukan hubungan jarakTPA terhadap kualitas air. Untuk menduga hubungan  dilakukan dengan analisis regresi dan korelasi antara tumpukan sampah, air lindidan kualitas air tanah. Hasil Kualitas air sumur di kecamatan Alak berada pada kategori sesuai artinya sesuai dengan Permenkes No. 492/Menkes/Per/IV/2010 tanggal 19 April 2010. Variabel jarak keberadaan tempat pembuangan akhir berpengaruh terhadap kualitas air sumur. Parameter kualitas air yang diambil dari daerah pemukiman di kelurahanAlakpadajarak 800 m dan 1.200 m masih dalam batasaman dikonsumsi. Sedangkan pada jarak 400 m dibutuhkan perhatian pemerintah untuk pengelolaan TPA secarabaik agar tidak berdampak pada kesehatan masyarakat.Kata kunci: Tempat Pembuangan Akhir, jarak TPA, kualitas airAbstract: Piles of garbage and leachate directly and indirectly can disturb the health of the environment and the balance of the aquatic ecosystem.This study aims to assess the physical, chemical and biological quality of well water at various distances from the Final Disposal Landfill and its impact on the health of the population. The study uses a stratified random sampling method that is based on the distance of 400 m-1200 m from the landfill from the location of groundwater well sampling.The results of sample analysis determine water quality by comparing Water quality standards. Analysis of the effect of landfill distance on water quality then calculated determination value (r2) determines the relationship between landfill distance to water quality.To estimate the relationship is done by regression analysis and correlation between the garbage heap and leachate and ground water quality. Results The quality of well water in Alak sub-district is in the appropriate category according to the meaning of Permenkes No. 492 / Menkes / Per / IV / 2010 April 19, 2010. Variable distance of the existence of the final disposal site affects the quality of well water. Water quality parameters taken from residential areas in Alak village at a distance of 800 m and 1,200 m are still within the safe limits for consumption. Meanwhile, at a distance of 400 m, government attention is needed to properly manage the landfill so it does not have an impact on public health.Keywords: Landfills, Landfill distance, Water quality
The Influence of Paddy Fields toward The Seasonal Herbaceous Wetland Ecosystem in Rawa Pening Lake Desti Christian Cahyaningrum
Jurnal Biologi Tropis Vol. 20 No. 2 (2020): Mei - Agustus
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (566.839 KB) | DOI: 10.29303/jbt.v20i2.1932

Abstract

Most of the tidal land in the lakeside of Rawa Pening is currently used for rice farming activities. This activity is thought to have negative impacts on seasonal herbaceous wetland ecosystem in this region. The study aimed to analyze the influence paddy fields toward the seasonal herbaceous wetland ecosystems in Rawa Pening Lake through an vegetation composition and structure approach. This study is a quantitative descriptive research with field observation method. Data of vegetation was collected using line intercept transect technique in the area widely of 625 m2 for each affected and unaffected ecosystem sample. Data analysis was performed by calculating the Sorensen Similarity Index (IS), the Margalef Diversity Index (R), the Shanon-Wiener Diversity Index (H'), the Dominance Index (C), and the Evenness Index (E). The results showed that there were differences in the composition and structure of vegetation on the two ecosystem samples, seen from the value of similarity index (IS) between both of them that included in the low criteria (39.85%). The results also showed that the Shanon-Wiener Diversity Index (H') and the Margalef Diversity Index (R) on the affected ecosystem sample  (H' = 1,9834; R = 1,825) are lower when compared to the unaffected ecosystem sample (H'= 2,1297; R = 2,112). So it can be concluded that the existence of paddy fields has changed the composition and structure of vegetation and reduced the diversity of vegetation in the affected ecosystem. Based on these conclusions, it is recommended to construct a sustainable management system of paddy fields on Rawa Pening Lake's tidal land as the effort for natural ecosystems maintenance in this region, especially in the biodiversity and ecological services preservation.
Aktivitas Antibakteri Ekstrak Metanol Daun Turi (Sesbania grandiflora L.) Terhadap Pertumbuhan Klebsiella pneumoniae Huurun Iien; Lalu Zulkifli; Prapti Sedijani
Jurnal Biologi Tropis Vol. 20 No. 2 (2020): Mei - Agustus
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.405 KB) | DOI: 10.29303/jbt.v20i2.1790

Abstract

Abstrak: Sesbania grandiflora (tanaman turi) memiliki berbagai khasiat dalam pengobatan penyakit termasuk yang dapat digunakan sebagai campuran dalam persiapan bahan antibakteri. Tumbuhan ini mengandung senyawa metabolit sekunder yang dapat memiliki aktivitas sebagai zat antibakateri. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder ekstrak methanol daun turi dan mengetahui bioaktivitas ekstrak tersebut terhadap pertumbuhan bakteri Klebsiella pneumoniae. Daun S. grandiflora diekstraksi dengan metode maserasi menggunakan pelarut metanol. Hasil uji fitokimia secara kualitatif menunjukkan bahwa ekstrak metanol daun turi mengandung senyawa metabolit sekunder seperti tannin, saponin, dan terpenoid. Uji penghambatan sifat antibakteri dilakukan berdasarkan metode difusi sumuran, dengan mengukur rata-rata zona hambat yang terbentuk pada media MHA. Hasil penelitian menunjukkan bahwa ekstrak metanol dari daun S. grandiflora dengan konsentrasi 10%, 25%, 40%, dan 55% menunjukkan diameter rata-rata zona hambatan masing-masing 7,2, 14,4, 17,9, dan 22,5 mm. Kemampuan untuk menghambat pertumbuhan bakteri pada konsentrasi 55%, 40%, dan 25% dikategorikan sebagai penghambatan kuat karena memiliki diameter zona hambat rata-rata lebih dari 12 mm (sesuai dengan kategori Vasanthakumari). Konsentrasi yang semakin tinggi memberikan efek penghambatan yang lebih kuat. Hasil di atas menunjukkan bahwa kandungan ekstrak daun tanaman turi dapat digunakan sebagai sumber bahan obat baru di masa depan melalui penelitian lebih lanjut.Kata kunci: Ekstrak Daun Turi, maserasi, antibakteri, Klebsiella pneumoniae, metabolit sekunderAbstract: Sesbania grandiflora (Turi plant) has various medicinal properties, including as a mixture in the preparation of antibacterial ingredients. This plant contains secondary metabolites that can have antibacterial activity. This study aims to determine the secondary metabolite content of the turi leaf methanol extract and determine the bioactivity of the extract on the growth of Klebsiella pneumoniae. S. grandiflora leaves were extracted using the maceration method using methanol as a solvent. Phytochemical test results show that the turi leaf methanol extract contains secondary metabolites such as tannin, saponin, and terpenoids. The antibacterial inhibition test was carried out based on the well diffusion method, by measuring the average of the zone of inhibition formed on the MHA media. The results showed that the methanol extract of the leaves of S. grandiflora with concentrations of 10%, 25%, 40%, and 55% showed an average diameter of inhibition zones 7.2, 14.4, 17.9, and 22, 5 mm, respectively. The ability to inhibit bacterial growth at concentrations of 55%, 40%, and 25% were categorized as having a strong inhibitory power because it has an average inhibition zone diameter of more than 12 mm (according to the Vasanthakumari category). The higher concentration gives a stronger inhibitory effect. The above results show that the leaf of the turi plant can be used as a source of new medicinal ingredients in the future.  Keywords: Turi Leaf Extract, maceration, antimicrobial, Klebsiella pneumoniae, secondary metabolites

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