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Jurnal Kimia Terapan Indonesia

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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 08532788 EISSN : 25277669 DOI : -

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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Articles 283 Documents

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SIFAT DAYA SERAP AIR DAN STABILITAS PENYERAPAN AIR HIDROGEL POLIMER KOMPOSIT Muhamad Nasir
Jurnal Kimia Terapan Indonesia Vol 12, No 2 (2010)
Publisher : Research Center for Chemistry - LIPI

Hydrogel polymer composite has been synthesized by using 10L scale reactor with batch systems. Hydrogel was synthesized by radical polymerization of a mixture of carboxy methyl cellulose, acrylic acid and methylene bis acrylamide as crosslinker. Hydrogel polymer composite was prepared by adding bentonite to polymer hydrogel. Swelling ratio which indicates of water absorption of hydrogel polymer composite was 60 g / g with a standard deviation of 6.2 %. Durability and stability tests for water absorption of hydrogel polymer composite for 15 days showed the swelling ratio is almost constant and hydrogel is quite stable and not broken down quickly. Effect of temperature and salt solution on water absorption as follow; the water absorption at higher temperatures lower than room temperature and the water absorption from the salt solution is lower than the water absorption of pure water. These results show the hydrogel polymer composite is sensitive to temperature and salt solution.Keyword: hydrogel polymer composite, water absorption,hydrogel stability.

PENGARUH SUMBER KARBON TERHADAP PRODUKSI ENZIM INULINFRUKTOTRANSFERASE DARI Nonomuraea sp Yetti Mulyati I; Sri Pudjiraharti; Een Sri Endah; Tanaka tanaka; Teruo Sone; Asano K
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

The aims of this research is to produce inulinfructotransferase enzyme with potentially benefit of bacteria Actinomycetes which was isolated from Indonesia soils to produce Difructoanhydride-Ill (DFA III), a compound has physiology activities to enhance calsium absorption of rats. cows and human colons so that the materials can be applied as antiosteoporosis. The processing of inulinfructotransferase enzyme from Nonomuraea sp by usingshakeflask level and study the influence of carbohydrate source were conducted. This enzyme has optimum temperature at 65rtC,higher than optimum temperature which has found before and the activities was stable at 70dc for 20 minutes. So that the enzyme has potentially applicable for industry scale. The results indicated that optimation condition for processing inulinfructotransferase enzyme of Nonomuraea sp : temperature sore. incubation time 48 hours.carbon source of inulin 20 g/L, nitrogen source 5g/L, noyeast extract added, the enzyme activities was 7,3 Unit/ml; However,when medium contains yeast extract 0,2 gIL,the inulinfructotransferase enzyme activity was 14,6 Unit/mL.Keywords: Inulinfructotransferase enzyme, Nonomuraeasp.inulin. yeast extract, DFAII

KESTABILAN AKTIVITAS ANTIBAKTERI HASIL FERMENTASI MOLASE OLEH STREPTOMYCES RIMOSUS ATCC 33022 Sri Handayani; Krisanti Yuwati; A. T. Karossi
Jurnal Kimia Terapan Indonesia Vol 4, No 2 (1994)
Publisher : Research Center for Chemistry - LIPI

Molases and ammonium sulfate can be used as medium component to supply carbon and nitrogen respectively for production of antibacterial, through fermentation by Sirimosus ATCC 33022. During fermentation, pH, biomass and antibacterial activity against Bacillus cereus were observed. The results of this investigation indicated that the antibacterial production started after 48 hours of incubation and the maximum production was achieved after 144 hours of incubation. The antibacterial agent was extracted with n-butanol of technical grade and HCI 0.1 N and purification was carried out by recrystallization with ethanol as the solvent. The yield was 50.278 mg antibacterial agent per litre of fermentation medium. The isolated antibacterial compound possesses Amax of 266 nm whereas the oxytetracycline demonstrated maximum peak at 267nm with Eo 1.8529 x 10(4) cm(-l) M(-1). Thin layer chromatography with three eluents of the standard oxytetracycline gave Rf of 0.76; 0.64 and 0.75 while the isolated compound gave 0.76; 0.65 and 0.77 for the respective eluent. HPLC analysis on J-l-bondapak C-18 column with methanol-acetonitrile-oxalic acid 0.1 M (1 : 15 : 7) being the solvent indicated that the isolated antibacterial agent and oxytetracycline standard were eluted at 4.35 minutes. Further analysis with FTIR of both isolated antibacterial agent and oxytetracycline standard had peaks at 3400, 1650-1600, 1238 and 2916-2848 cm(-1) indicating the presence of fenol, amide, amine and methyl groups. Antimicrobial activity tests during storage at 4 °C and -12°C of the isolated compound in various HCI solutions showed that HCI 0.1 N gave the best stability.

THE DETERMINATION OF SUGARS BY CHROMATOGRAPHIC METHOD Sri Sumartini; Julia Kantasubrata
Jurnal Kimia Terapan Indonesia Vol 2, No 1-2 (1992)
Publisher : Research Center for Chemistry - LIPI

Experiments have been carried out to analyse sugars using TLC and HPLC methods, In the TLC method, separation of sugars was performed on silica plates impregnated with monosodium phosphate and using mixture of ethylacettuel pyridinde/water as an eluent. Whilst in the HPLC method, the use of three column types i.e. diol, RP-18 and modified silica column were tested. The results showed that TLC method was able to measure three sugars i:e. sucrose, glucose and fructose with standard deviations of 11.6%, 7,6% and 1,9%, respectively. On the other hand, the HPLC method with silica column modified by polyamine and compressed with WATERS RCM-l00, showed the best results, in which mixtures of nine sugars were well separated and measured quantitatively with good precisian.

Sintesis Selulosa Suksinat dalam Cairan Ion 1-Butil-3-Metilimidazolium Klorida ([BMIM]CL) dengan Metode Microwave Assisted Organic Synthesis (MAOS) dan Penentuan Derajat Substitusinya Tety Sudiarti; D Wahyuningrum; B Bundjali; I M Arcana
Jurnal Kimia Terapan Indonesia Vol 18, No 01 (2016)
Publisher : Research Center for Chemistry - LIPI

Selulosa merupakan polimer alam yang sangat melimpah dan digunakansecara luas sebagai bahan mentah untuk berbagai aplikasi industri sepertifiber, kertas, membran, cat dll. Modifikasi selulosa dengan anhidrida suksinatmerupakan salah satu cara untuk memberikan nilai tambah pada selulosatersebut. Untuk meningkatkan kemampuan melarutkan dan efisiensimodifikasi selulosa, digunakan cairan ion 1-butil-3-metilimidazolium klorida([BMIM]Cl) sebagai pelarut. Metode microwave assisted organic synthesis(MAOS) dipilih karena dapat mempercepat laju reaksi, meningkatkan yielddan mengurangi produk samping. Tujuan penelitian ini adalah mensintesisselulosa suksinat dalam [BMIM]Cl dengan metode MAOS serta menentukanfaktor-faktor yang mempengaruhi derajat substitusi (DS) selulosa suksinatyang dihasilkan. Selulosa dilarutkan dalam [BMIM]Cl dengan berbagaiperbandingan dan direaksikan dengan anhidrida suksinat pada perbandinganselulosa:anhidrida suksinat = 1:10 menggunakan reaktor microwave padaberbagai kondisi reaksi (waktu, suhu, daya iradiasi microwave). Berdasarkanpengukuran spektrum FTIR terhadap produk diperoleh bahwa selulosasuksinat berhasil disintesis yang ditandai dengan munculnya puncak barupada bilangan gelombang 1722,43â€“1726,29 cm-1 yang merupakan pitaserapan untuk C=O ester dengan derajat substitusi pada rentang 0,66 â€“ 1,33.Kondisi pelarutan dan kondisi reaksi mempengaruhi derajat substitusiselulosa suksinat yang dihasilkan. Makin banyak [BMIM]Cl yang digunakan,makin tinggi derajat substitusi selulosa suksinat yang dihasilkan.Keywords : cellulose succinate, ionic liquid, [BMIM]Cl, MAOS method, degree of substitution

RADIONUCLIDIC IMPURITIES IN PERTECHNETATE SOLUTION ELUTED FROM 99mTc-CHROMATOGRAPHIC GENERATOR LOADED WITH 99Mo-FISSION PRODUCT Sunarhadijoso Soenarjo; Adang Hardi Gunawan
Jurnal Kimia Terapan Indonesia Vol 7, No 1-2 (1997)
Publisher : Research Center for Chemistry - LIPI

Medical radioisotope of 99mTc was firstly produced in Indonesia through the nuclear reaction of 98Mo (n;y) 99Mo -> 99mTc. The separation of the resulting 99mTc from the postirradiated natural MoO3 was carried out by solvent extraction using methyl ethyl ketone. Instead of this method, Radioisotope Production Center (RPC) BATAN has routinely produced 99mTc-chromatographic generator loaded with 235U-flSsion-produced 99Mo to provide 99mTc. By such generator, 99mTc can be easily and repeatedly liberated in the form of pertechnetate by vacuum elution using saline solution. Some fission- produced radionuclides, however, potentially contaminate the pertechnetate fraction. Gamma spectrometric determination was carried out to evaluate the level of radionuclidic impurities contaminating 99mTc-pertechnetate solution eluted from 99mTc- chromatographic generator produced over a 10-month period in 1993 - 1994. Radioactivity yield of the resulting 99mTc was independent to the origin of the loaded 99Mo. The 99mTc-pertechnetatate fractions were frequently contaminated with 99Mo, 131I and 103Ru, but the contamination did not exceed maximum permissible level. The fluctuation of contamination level may be influenced by irradiation parameters and separation techniques applied to the production of the loaded 99Mo.

ANTICANCER ACTIVITY OF ETHANOLIC EXTRACT OF Selaginella plana HIERON. ON T47D CELL LINE IN VITRO Chandra Risdian; Sri Handayani; Zalinar Udin; Rina Andriyani; Marissa Angelina
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

Selaginella sp belongs to the Selaginellaceae family. It has been used in China and Indonesia as a traditional medicine. It hasseveral medicinal properties including antibacterial, anticardiovascular, and anticancer agent. The aim of the present study was to access the anticancer property of the ethanolic extracts of Selaginella plana Hieron. on T47D breast cancer cell line. The proliferation of T47D cell line was detected by SRB (Sulforhodamine B) assay which was measured at a wavelength of 515 nM. The result showed that the IC50 of the ethanolic extract was determined at 7.03 pg/mL. This significant activity was assumed due to its high total flavonoid content. The totalflavonoid content of the ethanolic extract was 23.04%. Flow cytometry analysis indicated that the extract may undergo the cell death via apoptosis pathway. In conclusion, the ethanolic extract of Selaginella plana Hieron: has considerable activity in inhibiting T47D cell line proliferation.Key words: Selaginella plana Hieron., SulforhodamineB, T47Dcells, anticancer flow cytometry

PENENTUAN RUNUTAN Cu, Cr, Mn dan Fe DALAM MATRIKS AIR LAUT DENGAN SPEKTROFOTOMETRI SERAPAN ATOM DAN EKSTRAKSI PELARUT Ardeniswan Ardeniswan; Sumardi Sumardi
Jurnal Kimia Terapan Indonesia Vol 3, No 1 (1993)
Publisher : Research Center for Chemistry - LIPI

A method has been studied for the analysis of trace metals such as Cu, Cr, Mn, Fe in artificial seawater matrix samples using atomic absorption spectrophotometric (AAS). Concentration of theSe metals was carried out by extraction using methyl isobutyl ketone (MIBK) after being reacted with complexing agents, and transfered into aqueous solution by backextraction using 4 N nitric acid. The metal complexing agents used were ammonium pyrrolidinedithiocarbamate (APDC), sodium diethyldithlocarbamate (Na-DDC), mixture of equal ratio of APDC and Na-DDC. From these study it was found that use of APDC gave recovery for Cu, Cr, Mn and Fe 104.4; 25.4; 0; 83.0 % respectively at pH 5, while use of Na-DDC gave 83.5; 89.0; 79.4 and 81.3 % , respectively at the same pH. A mixture of equal ratio of APDC and Na-DDC was found to gave recovery for Cu, cr, Mn and Fe of 104.6; 103.9; 102.1 and 101.2 %, respectively at pH 5.

POKOK-POKOK DALAM ANALISA MIKROBIOLOGI PANGAN*) Sebuah Tinjauan Milono Poesponegoro
Jurnal Kimia Terapan Indonesia Vol 7, No 1-2 (1997)
Publisher : Research Center for Chemistry - LIPI

Microorganisms in foods not only can deteriorate the quality and shelf life of foods, but also capable of producing food poisoning. Therefore, microbiological analysis of foods is essentia, particularly to establish safety, to secure adequate microbiological quality and for standardization of food products.The existence of microbial pollution in foods needs standard microbiological analysis of foods to obtain accurate results of analysis. This is due to the fact that beside the complex nature of foods, the number of factors affecting the results of analysis are innumerable such as food age, sampling methods and treatment of the samples, and the methods of analysis used. In framing me/hods for the microbiological analysis of foods consideration must be given not only to the phycical and chemical properties of the food, but also to the form of processing they have recieved, the types of pathogens or spoilage organisms likely to be of most importance and the number likely to be present. This paper presents a review on the fundamentals of microbiological analysis of foods to clarify the various microbiological aspects, background and the principles outlay the works of microbiological analysis offoods and their problems.This paper presents a review on the fundamentals of microbiological analysis of foods to clarify the various microbiological aspects, background and the principles outlay the works of microbiological analysis of foods and their problems.

ANTI JAMUR HASIL FERMENTASI Streptomyces Isp. 192 PADA MEDIA PATI DAN GLUKOSA S Djajasupena; O Suprijana; S.A Desak Gede; A T.Karossi
Jurnal Kimia Terapan Indonesia Vol 11, No 2 (2009)
Publisher : Research Center for Chemistry - LIPI

Streptomyces ISP 192 was family of Actinomycetes which was high value microorganism that industrially important because it was capablefor producing ofbioactive compound that very useful in the field of medicine that is antibiotic. Recently antibiotic is still eligible medicine to overcome infection disease, but many more organism that cause of infection have been resistant against various antibiotics so need to look for a new antibiotic. This research cover cultivation of Streptomyces ISP 192 in potato dextrose agar (PDA) medium then fermentation by 250 mL shakeflash with 50 ml working volume. Microorganism was inoculated in fermentation medium MF1 and MF2 that contain (%)w/v variation of starch and glucose to bea source of carbon as soon as (%)v/v variation of inoculum. Fermentation process was conducted in 30°C temperature with 150 rpm shaking for seven days. Regularly for each 24 hour, sample was taken for analyzing ofpH, glucose and protein concentration, dry weight of cell and testing of anti fungal activity against Candida albicans, Microsporum gypseum, Tricophyton sp. dan Aspergillus niger. The result of research showed Streptomyces ISP 192 has high anti fungal activities against Candida albicans, Microsporum gypseum, Tricophyton sp., in usage of fementation media MF2 with 10 % glucose to be a carbonsource and 10% inoculum but not have activited against Aspergillus niger. Biosynthesis of antibiotic anti fungal start from 4th to 6th day that indication indecreasing of glucose and protein concentration as well aspH.Keywords: Streptomyses ISP 192, fermentation, testing ofantifungal activities.

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