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Jurnal Kimia Terapan Indonesia

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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 08532788 EISSN : 25277669 DOI : -

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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Articles 283 Documents

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EFFECTS OF TRACE METALS AND MEDIUM COMPOSITION ON THE GROWTH OF Aspergillus niger ATCC 11414, IN A SUBMERGED CULTURE Milono Poesponegoro; Oei Ban Liang
Jurnal Kimia Terapan Indonesia Vol 3, No 2 (1993)
Publisher : Research Center for Chemistry - LIPI

In an attempt to optimize citric acid fermentation, a study has been conducted to determine optimum nutritional conditions for the growth of Aspergillus niger ATCC 11414. The objective of the study was to obtain information on the growth of this strain in a submerged culture for the study of citric acid production The following article summarizes the results of study on the effects of trace metals and composition of chemically defined medium on mycelial growth of A.niger ATCC 11414. Chemically defined media containing glucose as the carbon source and energy were used throughout the work. Growth experiments were carried. Out by a submerged culture process, in a 300-ml Erlenmeyer flask which contained 50ml liquid medium. The process was conducted at 30°C for 4 days in an orbital shaker incubator operated at 200 rpm. The cultivation process was followed by monitoring the changes in the culture medium of the concentrations of biomass, total reducing sugars, citric acid, and pH of the medium. It was concluded that copper (II), iron (II), zinc (II), and manganese (II) ions had a remarkable effect on the growth of A. niger ATCC 11414. With 5% glucose, the study showed that 5 - 15 ppm copper, 0.5 - 25 ppm iron and 0.5 - 25 ppb manganese ions were optimal for the growth of the strain. The growth of the strain increased with the increase of Zn2+ added (0.5 - 25 ppm). The most optimal medium for the growth of A.niger ATCC 11414 was found to be able to produce more than 16 g of dry weight of biomass for 50 g glucose.

ADSORPSI ION MN(II) PADA ZEOLIT DARI ABU DASAR BATUBARA TERMODIFIKASI DITIZON Riandy Putra; Khamidinal Khamidinal; Didik Krisdiyanto; Irwan Nugraha
Jurnal Kimia Terapan Indonesia Vol 17, No 2 (2015)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jkti.v17i2.25

Adsorption characteristic of Mn(II) on zeolite synthesized from bottom ash with alkaline hydrothermal conditions loaded with dithizone have been examined. The parameters studied includes effect of pH, contact time, initial concentrations and temperature of Mn(II) by batch experiments. The raw and modified samples were characterized by XRF, XRD, FT-IR and SAA. The results of XRD and FTIR showed that hydrothermal product consists of a mixture of zeolite (zeolite Y, zeolite Na-P and zeolite X) and sodalite crystals. Hydrothermal product showed specific IR absorption zeolite at wavenumber 300-1250 cm-1 and zeolite modified dithizone showed absorption -NH and â€“SH at wavenumber 1496,76 and 2461,17 cm-1. The results SAA showed that with addition dithizone lowering the specific surface area of zeolite from 160,262 mÂ²/gÂ to 69,609 mÂ²/g. Results of study show that adsorption of Mn(II) reaches its maximum values at pH 6 for zeolite and pH 8 for zeolite modified dithizone, respectively. The adsorption kinetics based on the pseudo-second-order rate equation with adsorption rate constants for zeolite synthesis -0,014 g/mg.min-1and for the zeolite modified dithizone 0,0204 g/mg.min-1. Based on the Langmuir equation, the maximum adsorption capacity (qmax) synthetic zeolite of 14,493 mg/g and zeolite modified dhitizone 15,873 mg/g. Termodynamic parameter indicated thatadsorption using two adsorbents takes place spontaneously at a lower temperature.Keywords: Bottom ash, zeolite, hydrothermal, dithizone, adsorption

ISOLATION AND SCREENING OF FLOC-PRODUCING MICROORGANISMS Milono Poesponegoro; Roy Heru Trisnamurti
Jurnal Kimia Terapan Indonesia Vol 8, No 1-2 (1998)
Publisher : Research Center for Chemistry - LIPI

All of the aerobic biological wastewater treatmenr systems depend upon the flocculation of the microorganisms and their separation from the liquid phase for complete stabilization. Therefore, aside from the metabolic characteristics of the microorganisms, the most important characteristic is their ability to flocculate.A study on the sludge .flocculation, isolation and screening of floc-producing microorganisms for biological wastewater treatment processes has been carried out. Microrganisms were olated from activated sludges obtained from local food industries. It was found that biofl occul ation of sludge was affected by the type and concentration of carbonaceous substrate. The substrates used were glucose, acetate and starch, at four levels of concentration, i.e. 1.0, 2.5, 5.0 and 25.0g/l. The activated sludge (Sludge-I) found better in bioflocculation than ther two sludge tested (Sludge-C and Sludge-K) was used for this purpose. The results showed that the Sludge-I gave better bioflocculation either on glucose or starch at a concentration of 1.0g/l than on acetate and other concentrations tested.The isolates (27 strains) obtainedfrom the activated sludge were then screened for their ability to flocculate and their acitivity in organic degradation. It showed that some of them produced good biofloc and gave high percentage of COD removal (more than 80%). The screening was carried out using shake-flask culture technique at 30°C for 10 days. Out of 27 strains tested, Strain-Ivb and Strain-Ivk were found suitable as inoculum candidates for the biological wastewater treatment since they produced granular floc which flocculated readily and gave high percentage of COD removal, around 85%, after 10 days of incubation. Tentatively both lsolate-Ivb and Isolate-Ivk have been identified as Candida sp.It was revealed that Strain-lvb and Str ain-Ivk gave better bioflocculation and percentage of COD removal, compared to 3 lands commercial inoculum tested. The commercial inocula produced pin-point floc with percentage of COD removal of 86 - 92%, while Strain-Ivb and Strain-Ivk produced granular floc with percentage of COD removal of 94% and 93% respectively. The results of study shows that continuous biological treatment of artificial wastewater containing starch (400 mg/l) using strainlvb gave percentage of COD removal of 77 - 96% (average value = 87%) at hydraulic retention time of 13 - 25 hrS (average value= 18 hrs); while strain-lvk gave percentage of COD removal of 84 - 95% (average value= 92%) at hydraulic retention time of 8 - 16 hrs (average value= 12 hrs). Both strains formed biofloc which settled readily.

ISOLASI DAN IDENTIFIKASI SENYAWA FLAVON DARI EKSTRAK ETIL ASETAT BRUCEA JAVANICA Merrill Megawati Megawati; A Darmawan; M Angelina; S Fajriah; M Hanafi; P D Lotulung
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

Buah Makasar (Brucea javanica Merrill)is a wild ornamental plant and one of the traditional medicine plants. Isolation andpurification of ethyl acetate extract of B.javanica using silica gel column chromatography with ethyl acetatem-hexane as a mobile phase gradually obtained a pure compound as yellow crystals. It was identified by using infrared spectrophotometer (IR), LC-MS (liquid chromatography mass spectrometer) and ID- and 2D-NMR (nuclear magnetic resonance), compound a identified as 6-hydroxy-2-(3,4,5-trihydroxyphenyl}-4H-chromen-4-one, a flavones compound with molecular formula Cl5HI006 and molecular weight of 287.18 m/z.Keywords: Brucea javanica Merrill, buah makasar, flavones,6-hydroxy-2-(3,4,5-trihydroxyphenyl)-4H-chromen-4-one

PENINGKATAN NILAI DAYA CERNA DAN ENERGI METABOLISME NITROGEN TERKOREKSI BIJI KACANG Vicia faba var. Diana MELALUI PENYINARAN DENGAN SINAR INFRA MERAH Patuan L.P. Siagian
Jurnal Kimia Terapan Indonesia Vol 3, No 2 (1993)
Publisher : Research Center for Chemistry - LIPI

Determination of the apparent digestibility by the difference method has been performed in order to ascertain the influence of infra-red treatment on the nutritional effect of legume seeds Vicia faba L. var. Diana. For the test, a total 0f 96 five-week old male chicks of LSL-Brown were kept in a full automatically adjustable climate conditioned room. The digestion test consisted of eight rations in mash form (1 basal ration and 7 test rations). The water was offered ad libitum. The duration of adaptation period was 4 days, the preliminary period was 3 days, and the main experimental period was 4 days, repective/y. The results of this experiment indicated a significant increase (p<0.05) of nutrient digestibility of legume seeds treated by infrared level 2 (80 seconds, seeds temperature 105°C) for dry matter (+14.6%), organic matter (+17.6%), starch (+16.5%), nitrogen free extract (+11.6%), lysine (+4.2%), threonine (+5.3%), and cystine (+11.9%). The value of nitrogen balance increased from 479,7 mg Niday to 622,3 mg Niday. An increase 0f N corrected meta-bolizable energy of the seeds from 10.67 MJ/kg DM to 11.95 MJ/kg DM was observed.

PHYSICAL AND CHEMICAL PROPERTIES OF ORGANIC MINERAL ADDITIVE FOR RUMINANT THROUGH BIOLOGICALLY INCORPORATED BY SACCHAROMYCES CEREVISIAE IN DIFERENCE SUBSTRATES Ahmad Sofyan; Hendra Herdian; Awistaros Angger Sakti; Gumilang Khairulli; Anuraga Jayanegara
Jurnal Kimia Terapan Indonesia Vol 17, No 2 (2015)
Publisher : Research Center for Chemistry - LIPI

This research was conducted to evaluate physical and chemical properties of organic mineral additive incorporated by Saccharomyces cerevisiae which was cultivated on different growth media consisted of cassava flour and rice bran, respectively. Treatment was arranged on completely randomized design consisted of rice bran without inoculants (RBo), rice bran with inoculants (RBi), cassava flour without inoculants (CFo), cassava flour with inoculants (CFi). Substrates were fortified by microminerals contained of Cu (100 ppm), Mn (100 ppm), Zn (100 ppm), I (10 ppm), Fe (2.5 ppm) and Co (2 ppm). Inoculation of S. cerevisiae could reduce fungal contamination. Physical characteristic of cassava flour was better in flavour and texture than rice bran in which tends to rancidity. Nutrients composition was similar in cassava and rice bran however crude fibre content in rice bran tends to be higher after fermentation. Mineral content (Zn and Fe) relatively decreased and in substrate supplemented by inoculant and rice bran had higher than cassava. In summary, micromineral was incorporated in cassava flour with inoculated S. cerevisiae had better than rice bran.Keywords: Organic mineral, cassava flour meal, S. cerevisiae, rice bran, ruminant.

PREPARATION OF MgO-CeO2 MIXED OXIDE WITH IONIC LIQUID AS CATALYST FOR DIMETHYL CARBONATE SYNTHESIS VIA TRANSESTERIFICATION Haznan Abimanyu; Kye Sang Yoo; Byoung-Sung Ahn
Jurnal Kimia Terapan Indonesia Vol 12, No 2 (2010)
Publisher : Research Center for Chemistry - LIPI

The synthesis and application of dimethyl carbonate (DMC) are achieving increasing importance due to its low toxicity and versatile reactivity. The phosgenation-route has been losing attraction recently due to the use of virulent phosgene. In transesterification process, DMC is co-generated with ethylene glycol (EG). In this study, various ionic liquids were used as template in coprecipitation methods to prepare mesoporous MgO-CeO2 mixed oxides particles. Among the ionic liquids, [Bmim][BF6] displayed the best performance in terms of activity, while [Omim][PF6] obtained the best selectivity for this reaction. The addition of IL's in the coprecipitation method increased the surface areaand pore volume of the catalysts. Meanwhile, the crystallite size of the catalysts was reduced many times. However, there is no effect of the surface areaand particle size as well on the catalytic activity of the catalyst in this reaction. The activity and selectivity of the catalyst depend on the base strength distribution. The moderate basic site is responsible for the catalytic activity, while the selectivity is more dependableon the strong basic site. Keyword: preparation, MgO-CeO2, ionic liquid, dimethyl carbonate, coprecipitati

EKSTRAK TERSTANDAR SECARA KIMIA DAUN Brucea javanica Merrill Marissa Angelina; Abdul Mun'im; M Hanafi
Jurnal Kimia Terapan Indonesia Vol 13, No 2 (2011)
Publisher : Research Center for Chemistry - LIPI

The preparation of Brucea leaves have been done for specific parameters and non specific parameter laboratory worb refers to TheNational Agency of Drug and Food Control Regulation for extract quality control. The result for specific extract parameters were the rendement of extraction is 28%, the values of water- extractive and ethanol-extractive are 11.49% and 9.41%, respectively. The result of determination of the non specific parameters of the extract are; loss on drying is 18.95%, the water content is 15.06%, the total ashes is 16.12%. and the level of ashes not dissolved in acid is 11.14%. The analysis of chemical compound shows that the extract containedflavonoid, tannin, and glycoside. For the quantitative control has been measured the level of totalflavonoid wherethe result is 9.901%Keywords : Preparation, Brucea; Specific parameter, non specificparameter, extract

PHOTOREACTION OF a, B - ENONES IN METHANOL IN THE PRESENCE OF TITANIUM (IV) CHLORIDE P. Lotulung; T. Sato
Jurnal Kimia Terapan Indonesia Vol 2, No 1-2 (1992)
Publisher : Research Center for Chemistry - LIPI

When cyclic a, B-unsaturated carbonyl compounds were irradiated by ultraviolet from a high pressure mercury vapour lamp at 25 °C, in alcohols in the presence of titanium chloride, a coupling reaction between the carbonyl carbon atom and the a-carbon atom of the alcohol took place, producing dihydrofurans, monomethylether, acetals or aldehides, but in the case of a-methyl substituted enone it resulted in diol monomethylether due the two times occuring methanol substitution.

Isolasi Kandungan Senyawa Kimia dari Pakis Simpei (Cibotium barometz) serta Uji Bioaktivitas Antioksidan, Uji Toksisitas (BSLT) dan Antidiabetes Sri Hartati; Rugayah Rugayah; Titien Ng Praptosuwiryo
Jurnal Kimia Terapan Indonesia Vol 18, No 01 (2016)
Publisher : Research Center for Chemistry - LIPI

Cibotium barometz (L.) J. Sm. (Cibotiaceae) merupakan salah satu jenistumbuhan paku pohon komoditi ekspor yang memiliki nilai ekonomi tinggikarena kandungan kimianya yang dapat dimanfaatkan sebagai bahan obattradisional maupun modern. Penelitian ini ditujukan pada isolasi kandungankimia dan evaluasi anti oksidan, antidiabetes dan toksisitas ekstrak rimpangdan bulu C. barometz terhadap larva udang Arthemia salina L. (BSLT). Hasiluji antiokoksidan ekstrak metanol bulu dan rimpang serta hasil fraksionasidengan menggunakan metoda radical scavenger DPPH (Diphenyl PicrylHidrazyl), bahwa fraksi etil asetat dan butanol menunjukkan aktivitasberturut-turut dengan IC50 27,53 dan 46,24 ppm dan ekstrak metanol rimpangdan ekstrak metanol bulu dinyatakan kurang aktif dengan IC50 183,43 dan126,10 ppm. Hasil uji toksisitas dengan metoda Brine Shrimp Lethality Test(BSLT) dinyatakan tidak toksik dan hasil uji anti diabetes dengan metoda Î±-glukosidase juga tidak aktif. Hasil isolasi senyawa kimia ekstrak rimpang danbulu C. barometz dengan metoda kromatografi kolom gravitasi dandiidentifikasi berdasarkan data-data spektra IR dan RMI proton dan karbonserta LC-MS, ekstrak n-heksana rimpang dan ekstrak metanol bulu C.barometz, diperoleh senyawa metil dodekanoat (A), ÃŸ-sitosterol (B), ÃŸ-sitosterol-O- glukopiranosida (C) dan 2,3,4,5,6-pentahidroksi sikloheksanasam karboksilat (D).

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