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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Published by Universitas Jenderal Soedirman
ISSN : 08531629     EISSN : 08531629     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
Arjuna Subject : -
Articles 342 Documents
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Perubahan Lingkungan Ekosistem Sungai Belawan terhadap Kualitas Air dan Keanekaragaman Makrozoobenthos sebagai Bioindikator Mayang Sari Yeanny
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 1 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.1.190

Abstract

The sampling technique of this research was purposive random sampling with 4 stations. The results showed that there were 23 genera of macrozoobenthic, which were categorized into 3 phyla, 5 classes, 10 orders, 18 families. The highest density population index was shown by Terebralia 92,59  ind./m2 (station IV) and the lowest was by Littorinia, Natica, Telescopium, dan Oleacinar 3,70 ind/m2 (station IV). The diversity index of  macrozoobenthic was low, ranged from 0,86 to 1,85 and the evenness were high except station IV (0,40). Similarity index of stations I and II was 21,05, stations I and was III 42,85. This means that no resemblance was observed between those stations.. Stations II and III 53,33 were resemble. The pollution of diversity macrozoobenthic was mediumness, and station IV was heavyness of pollution. Macrozoobenthic as bioindicators was Planaria, physa, dan Sphaerium. Effected by quality of water such as; pH, DO, and COD
Aktivitas Antioksidan Ekstrak Tubuh Buah Jamur Paha Ayam (Coprinus comatus) dengan Pelarut Berbeda Agus Susanto; Nuniek Ina Nuniek; Nuraeni Ekowati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.075 KB) | DOI: 10.20884/1.mib.2018.35.2.566

Abstract

Coprinus comatus or with local name is  drum stick chiken mushroom that includes edible and medicinal mushroom. This mushroom contains bioactive compounds that have several pharmacological effects, like as antibacterial, anticancer, immunomodulator, anti-inflammatory, prevent diabetes and antioxidant. bioactive compounds includes in fruit body C. comatus can be obtained by extraction used solvent. The objective of this study was to determine whether the C. comatus fungus was potential as an antioxidant producer and to know which solvent produced the best antioxidant activity from the extract of the fruit body of C. comatus. The method used in this research is experimental with two treatments ie n-hexane and ethanol 70% solvent. Fruit body of C. comatus is extracted by maceration method, then the viscous extract obtained by phytochemical test and antioxidant activity test using DPPH method (1,1-diphenyl-2-picrihidazil). The results obtained are then analyzed using t-test for independent samples. The results showed that the average yield of ethanol extract 70% higher than n-heksan  were each 61.33% ± 9,21 and 14.8% ± 0,87. The test of total phenol extract ethanol was 70% higher than n-hexane , each with an average of 325,19 ppm ± 50,01 and 110,08 ppm ± 34,67. The result of antioxidant activity test using DPPH method of ethanol extract 70% and n-heksan has IC50 value 2,48 mg/ml and 3,86 mg/ml. Phytochemical test results for flavonoid test showed positive result for both samples extract, terpenoid test showed positive result in both samples , the tannin test showed negative results  in both samples.
Respon Pertumbuhan Spesies C3 dan C4 terhadap Cekaman Kekeringan dan Konsentrasi CO2 tinggi Hamim Hamim
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 3 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.3.174

Abstract

Growth response of two C3 (wheat and kale) and two C4 species (Echinochloa crusgallii and Amaranthus caudatus) were analyzed in response to drought stress under different CO2 concentrations, i.e. ambient (350 ppm) and double ambient (700 ppm).  Drought was given by withholding water for several days until the plants were wilted.  Drought stress reduced relative water content (RWC), water potential (Yw), osmotic potential (Ys), net photosynthesis (Pn) and plant growth of all species.  During moderate drought Pn of C3 species decreased considerably, while that of C4 species was still sustained, suggesting that C4 cycle is important to sustain Pn during the beginning of the drought.  Doubled [CO2] enhanced growth of all species indicated by increase of plant height and leaf area, but the growth of C3 species was higher than that of C4 one.  Increase of growth due to high [CO2] was associated with an increase of Pn in C3 species but not in C4 species.  High [CO2] caused C4 species had RWC, Yw and Ys higher than C3 species during drought stress.  Increase of growth in C4 species may be associated by an increase of plant water status due to high [CO2].    
Kinetika Enzim Nitrilase Dari Sel Utuh Rhodococcus spp Pada Biotransformasi Mandelonitril R. Haryo Bimo Setiarto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 2 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.2.266

Abstract

Rhodococcus spp can be used as mandelonitrile substrate for carbon and nitrogen source in life cycle of metabolism. They have potential of activity biotransformation for produced (R/S)-mandelic acid from mandelonitrile. (R/S)-mandelic acid is an important biotransformation product for production of pharmaceuticals such as semisynthetic penicillins, cephalosporins, antitumor agents, antiobesity agents and antiinflamation agents. This research was conducted to determine the enzyme kinetics (Km and Vmax) of nitrilase from Rhodococcus TPIK, LP3, GLB5 (induction acetonitrile 1000 mM - mandelonitrile 100 mM) with florometric methods, spectrophotometer analysis (λ = 413 nm). This research was carried out by assaying nitrilase enzyme activities in various concentration of mandelonitrile subtrates which were between 10 mM – 100 mM with 10 mM interval. The result showed that the enzyme kinetics of nitrilase from Rhodococcus TPIK (Km was 72.303 mM and Vmax was 2.075 mM/ml cell/minute), Rhodococcus LP3 (Km was 47.048 mM and Vmax was 1.942 mM/ml cell/minute), Rhodococcus GLB5 (Km was 34.375 mM and Vmax was 2.083 mM/ml cell/minute). Nitrilase enzyme from Rhodococcus GLB5 have smallest Km value. So we can interpretationed, this enzyme have good complexity Enzyme-Substrate, high affinity with substrate, and high speed reaction for forms product mandelic acid.
Profil RAPD Tanaman Kantung Semar Beberapa Koleksi Kebun Raya Baturraden Retno Mayangsari; Agus Hery Susanto; Alice Yuniaty
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.2.484

Abstract

Telah dilakukan penelitian tentang profil RAPD beberapa tanaman kantung semar (Nepenthes spp.) koleksi Kebun Raya Baturraden. Tujuh individu yang dipelajari merupakan spesies semiendemik Gunung Slamet dan endemik Jawa, yaitu N. adrianii dan N. gymnampora. Metode yang digunakan adalah survei dengan teknik pengambilan sampel secara acak. DNA genomik sampel diekstraksi untuk kemudian digunakan sebagai templat dalam amplifikasi marka RAPD menggunakan teknik PCR dengan 10 primer acak, yaitu ES10G23, ES10A26, ES10C24, ES10G33, OPA-2, OPA-9, OPA-13, OPB-3, OPB-5, dan OPB-7. Hasil penelitian menunjukkan bahwa ke-10 primer tersebut menghasilkan 43 (67,2%) pita polimorfik dan 21 (32,8%) pita monomorfik dengan ukuran berkisar dari 130 hingga 1.500 bp. OPA-2 and OPB-3 menghasilkan polimorfisme tertinggi, sedangkan ES10G23 dan OPA-13 menghasilkan pita monomorfik terbanyak. Dendogram yang dikonstruksi menggunakan  UPGMA dalam MEGA 6.06 memperlihatkan bahwa dua klaster terbentuk pada jarak genetik 18%. Kedua klaster tersebut cenderung dipisahkan oleh lokasi geografi.
Kandungan Bahan Aktif Fomesafen dalam Biji Kedelai dan Kacang Hijau dari Tanaman yang Ditumbuhkan pada Tanah Bekas Paparan Reflex Murni Dwiati; Iman Budisantoso
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 1 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.1.78

Abstract

A research has been conducted to understand the responses of soybean and mungbean to the persistence of Reflex residuals in soils referring to its active substance contents, i.e. fomesafen, in the plants grown in Reflex-exposed soils. The study was carried out experimentally in glass house from June to November 2003 employing Completely Randomized Design (CRD) with treatments arranged factorial. Two factors were used, the first of which was plant species (soybean and mungbean) while the second was Reflex concentrations (0, 25, 50, 100, and 200 ppm). Each treatment combination was replicated three times. The parameters examined comprised of fomesafen contents in the seeds, number of seeds per pod,  weight of 100 seeds, and weight of total seeds. To measure fomesafen contents in the seeds, Gas Liquid Chromatograph with ECD detector and column containing 10% OV-101 chromosorb WHF 80/100 mesh of 2m lengths and 3 mm diameter was applied. Fomesafen residuals in the soils of 5 month persistence had no harmful effect on both soybean and mungbean plants. As well, they had no significant effect on the production variables of both plants. Soybean seeds planted in the 5-month Reflex-exposed soils still contained fomesafen while the corresponding mungbean seeds did not.
Kebiasaan Makanan Ikan Beloso (Saurida undosquamis) di Perairan Laut Cina Selatan Bagian Selatan (LCS) Moh. Rasyid Ridho; Richardus F. Kaswadji; Indra Jaya; Subhat Nurhakim
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 1 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.1.181

Abstract

The objective of this research is to understand the food habit of the lizard fish (Saurida undosquamis) in Southern South China Sea.For this purpose, purposive random sampling was conducted over 19 stations in trawlable bottom waters using swept area method and sample of 250 of S. undosquamis were examined for their stomach content. The lengths of S. undosquamis under this observation were 15,0-26,8 cm. The result showed that the S. undosquamis is demersal fish that distributed diurnally. The food habit of the S. undosquamis in South China Sea was different between the day and the night. The food habit of S. undosquamis in the day are mainly small demersal fishes, especially Leiognathus leusiscus and Upeneus sulphureus that have the same diurnal distribution pattern as  S. undosquamis. Meanwhile, during the night time, the food habit of  S. undosquamis was dominated by shrimps.
Kajian Histologis Infeksi LD50 SLNPV terhadap Kerusakan Membran Peritrofik Larva Spodoptera litura Fabricius Yayan Sanjaya; Nanin Diah; Wardono Niloperbowo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 3 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.3.273

Abstract

The effect of SlNPV infection on  peritrophic membrane  of Spodoptera litura has been carried out. The damage of histological structure caused by SlNPV (0, 315, 390, 465, 540 and 615 PIB/ml) was investigated after 0, 12, 24 ,72 and 96 hours post infection. The histological material was prepared by using parafin method after fixation with Bouin Solution, then sliced into 7 mm and stained with Hematoxilin-Eosin.   The descriptive obsevation on structural intact of peritrophic membrane histology caused by SlNPV infection shows a tendency to decrease, while in control, there was no damage at all.  The longer the exposition of virion in the midgut lumen the more damage on peritrophic membrane occurred. The severest damage occurred 96 hour after infection. The result prove that haNPV virion can destroy histological structure of midgut
Status Kesehatan dan Uji Spesies Indikator Biologi Ekosistem Mangrove Teluk Yotefa Jayapura John Dominggus Kalor; Lisiard Dimara; Ottouw G. Swabra; Kalvin Paiki
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2018.35.1.495

Abstract

This research was conducted to test the mangrove crab of Sesarmidae family as bio-indicator to assess health status of mangrove ecosystem in Youtefa Bay of Jayapura. Conducted since March-August 2017. Conducted in 3 stations namely St. Enggros, St.Tobati, and St. Nafri. Using the quadratic transect method (1x 1 m) and the quadratic transect (10 x 10 m). Data were analyzed using index of diversity, dominance, evenness, abundance, density, simple linear regression and test criteria of indicator species. The result of the research found the level of mangrove diversity in Enggros Station with the value of index 1.9 then Tobati and Nafri Station of 1, 4 with abundant diversity level. The density of mangroves in the three stations is obtained by Enggors: 1455.6 trees / Ha, Naftri: 1477.8 trees / Ha, and Tobati 1033,5 trees / ha with moderate density categories and good ecosystem health conditions. The biological criteria indicator test shows that the Sesarmidae crab meets the six criteria of the indicator species, so that it can be designated as a species indicator of the health status of the ecosystem.
Peningkatan Kualitas Akuakultur Menggunakan Teknologi Biofilter Mikroalga Imobil Rini Riffiani
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.831 KB) | DOI: 10.20884/1.mib.2009.26.3.165

Abstract

Page 7 of 35 | Total Record : 342

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